Dermal mesenchymal stem cells (DMSCs) inhibit skin-homing CD8+ T cell activity, a determining factor of vitiligo patients' autologous melanocytes transplantation efficiency
We here investigated the efficiency of autologous melanocyte transplantation of 23 vitiligo patients by focusing on perilesional skin homing CD8+ T lymphocytes, and studied the potential effect of dermal mesenchymal stem cells (DMSCs) on CD8+ T cell activities in vitro. Out of 23 patients with the a...
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| description | We here investigated the efficiency of autologous melanocyte transplantation of 23 vitiligo patients by focusing on perilesional skin homing CD8+ T lymphocytes, and studied the potential effect of dermal mesenchymal stem cells (DMSCs) on CD8+ T cell activities in vitro. Out of 23 patients with the autologous melanocyte transplantation, 12 patients (52.17%) had an excellent re-pigmentation, 6 patients (26.09%) had a good re-pigmentation, 5 patients (21.74%) had a fair or poor re-pigmentation. CD8+ T cells infiltrating was observed in the perilesional vitiligo area of all patients. Importantly, the efficiency of the transplantation was closely associated with skin-homing CD8+ T cell activities. The patients with high number of perilesional CD8+ T cells or high level of cytokines/chemokines were associated with poor re-pigmentation efficiency. For in-vitro experiments, we successfully isolated and characterized human DMSCs and skin-homing CD8+ T cells. We established DMSCs and CD8+ T cell co-culture system, where DMSCs possessed significant inhibitory effects against skin homing CD8+ T lymphocytes. DMSCs inhibited CD8+ T cells proliferation, induced them apoptosis and regulated their cytokines/chemokines production. Our results suggest that vitiligo patients' autologous melanocytes transplantation efficiency might be predicted by perilesional skin-homing CD8+ T cell activities, and DMSCs might be used as auxiliary agent to improve transplantation efficacy. |
| doi_str_mv | 10.1371/journal.pone.0060254 |
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Out of 23 patients with the autologous melanocyte transplantation, 12 patients (52.17%) had an excellent re-pigmentation, 6 patients (26.09%) had a good re-pigmentation, 5 patients (21.74%) had a fair or poor re-pigmentation. CD8+ T cells infiltrating was observed in the perilesional vitiligo area of all patients. Importantly, the efficiency of the transplantation was closely associated with skin-homing CD8+ T cell activities. The patients with high number of perilesional CD8+ T cells or high level of cytokines/chemokines were associated with poor re-pigmentation efficiency. For in-vitro experiments, we successfully isolated and characterized human DMSCs and skin-homing CD8+ T cells. We established DMSCs and CD8+ T cell co-culture system, where DMSCs possessed significant inhibitory effects against skin homing CD8+ T lymphocytes. DMSCs inhibited CD8+ T cells proliferation, induced them apoptosis and regulated their cytokines/chemokines production. Our results suggest that vitiligo patients' autologous melanocytes transplantation efficiency might be predicted by perilesional skin-homing CD8+ T cell activities, and DMSCs might be used as auxiliary agent to improve transplantation efficacy.</description><identifier>ISSN: 1932-6203</identifier><identifier>EISSN: 1932-6203</identifier><identifier>DOI: 10.1371/journal.pone.0060254</identifier><identifier>PMID: 23577097</identifier><language>eng</language><publisher>United States: Public Library of Science</publisher><subject>Adult ; Apoptosis ; Autografts ; Biology ; Bone marrow ; CD8 antigen ; CD8-Positive T-Lymphocytes - immunology ; CD8-Positive T-Lymphocytes - pathology ; Cell culture ; Cell growth ; Cell Proliferation ; Cell Transplantation ; Chemokines ; Chemokines - biosynthesis ; Chemokines - metabolism ; Cytokines ; Cytotoxicity ; Dermatology ; Efficiency ; Epidermis - immunology ; Epidermis - metabolism ; Epidermis - pathology ; Female ; Gene Expression Regulation - immunology ; Homing ; Hospitals ; Humans ; Lymphocytes ; Lymphocytes T ; Male ; Medical research ; Medicine ; Melanocytes ; Melanocytes - transplantation ; Mesenchymal stem cells ; Mesenchymal Stromal Cells - cytology ; Mesenchyme ; Nitric oxide ; Patients ; Pigmentation ; Pigmentation - immunology ; Skin ; Skin - cytology ; Skin - immunology ; Skin - metabolism ; Skin - pathology ; Stem cells ; Studies ; T cells ; Transplantation ; Transplantation, Autologous ; Transplants & implants ; Vitiligo ; Vitiligo - immunology ; Vitiligo - metabolism ; Vitiligo - surgery</subject><ispartof>PloS one, 2013-04, Vol.8 (4), p.e60254-e60254</ispartof><rights>COPYRIGHT 2013 Public Library of Science</rights><rights>2013 Zhou et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License: https://creativecommons.org/licenses/by/4.0/ (the “License”), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><rights>2013 Zhou et al 2013 Zhou et al</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c593t-f360d028a4e049710f7b81550b0064b85e5839615a866ae741ade331362e55703</citedby><cites>FETCH-LOGICAL-c593t-f360d028a4e049710f7b81550b0064b85e5839615a866ae741ade331362e55703</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC3618417/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC3618417/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,723,776,780,860,881,2096,2915,23845,27901,27902,53766,53768,79342,79343</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/23577097$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Zhou, Miao-ni</creatorcontrib><creatorcontrib>Zhang, Zhi-qing</creatorcontrib><creatorcontrib>Wu, Ji-long</creatorcontrib><creatorcontrib>Lin, Fu-quan</creatorcontrib><creatorcontrib>Fu, Li-fang</creatorcontrib><creatorcontrib>Wang, Sui-qan</creatorcontrib><creatorcontrib>Guan, Cui-ping</creatorcontrib><creatorcontrib>Wang, Hong-lin</creatorcontrib><creatorcontrib>Xu, Aie</creatorcontrib><title>Dermal mesenchymal stem cells (DMSCs) inhibit skin-homing CD8+ T cell activity, a determining factor of vitiligo patients' autologous melanocytes transplantation efficiency</title><title>PloS one</title><addtitle>PLoS One</addtitle><description>We here investigated the efficiency of autologous melanocyte transplantation of 23 vitiligo patients by focusing on perilesional skin homing CD8+ T lymphocytes, and studied the potential effect of dermal mesenchymal stem cells (DMSCs) on CD8+ T cell activities in vitro. Out of 23 patients with the autologous melanocyte transplantation, 12 patients (52.17%) had an excellent re-pigmentation, 6 patients (26.09%) had a good re-pigmentation, 5 patients (21.74%) had a fair or poor re-pigmentation. CD8+ T cells infiltrating was observed in the perilesional vitiligo area of all patients. Importantly, the efficiency of the transplantation was closely associated with skin-homing CD8+ T cell activities. The patients with high number of perilesional CD8+ T cells or high level of cytokines/chemokines were associated with poor re-pigmentation efficiency. For in-vitro experiments, we successfully isolated and characterized human DMSCs and skin-homing CD8+ T cells. We established DMSCs and CD8+ T cell co-culture system, where DMSCs possessed significant inhibitory effects against skin homing CD8+ T lymphocytes. DMSCs inhibited CD8+ T cells proliferation, induced them apoptosis and regulated their cytokines/chemokines production. Our results suggest that vitiligo patients' autologous melanocytes transplantation efficiency might be predicted by perilesional skin-homing CD8+ T cell activities, and DMSCs might be used as auxiliary agent to improve transplantation efficacy.</description><subject>Adult</subject><subject>Apoptosis</subject><subject>Autografts</subject><subject>Biology</subject><subject>Bone marrow</subject><subject>CD8 antigen</subject><subject>CD8-Positive T-Lymphocytes - immunology</subject><subject>CD8-Positive T-Lymphocytes - pathology</subject><subject>Cell culture</subject><subject>Cell growth</subject><subject>Cell Proliferation</subject><subject>Cell Transplantation</subject><subject>Chemokines</subject><subject>Chemokines - biosynthesis</subject><subject>Chemokines - metabolism</subject><subject>Cytokines</subject><subject>Cytotoxicity</subject><subject>Dermatology</subject><subject>Efficiency</subject><subject>Epidermis - immunology</subject><subject>Epidermis - metabolism</subject><subject>Epidermis - pathology</subject><subject>Female</subject><subject>Gene Expression Regulation - immunology</subject><subject>Homing</subject><subject>Hospitals</subject><subject>Humans</subject><subject>Lymphocytes</subject><subject>Lymphocytes T</subject><subject>Male</subject><subject>Medical research</subject><subject>Medicine</subject><subject>Melanocytes</subject><subject>Melanocytes - transplantation</subject><subject>Mesenchymal stem cells</subject><subject>Mesenchymal Stromal Cells - cytology</subject><subject>Mesenchyme</subject><subject>Nitric oxide</subject><subject>Patients</subject><subject>Pigmentation</subject><subject>Pigmentation - immunology</subject><subject>Skin</subject><subject>Skin - cytology</subject><subject>Skin - immunology</subject><subject>Skin - metabolism</subject><subject>Skin - pathology</subject><subject>Stem cells</subject><subject>Studies</subject><subject>T cells</subject><subject>Transplantation</subject><subject>Transplantation, Autologous</subject><subject>Transplants & implants</subject><subject>Vitiligo</subject><subject>Vitiligo - immunology</subject><subject>Vitiligo - metabolism</subject><subject>Vitiligo - surgery</subject><issn>1932-6203</issn><issn>1932-6203</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2013</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>BENPR</sourceid><sourceid>DOA</sourceid><recordid>eNptUl1v0zAUjRCIjcE_QGCJB4agxY4_84I0tXxMGuKB8Ww5jtN6JHaJ3Un9T_xIbtZsWtGUhzi-55x778kpipcEzwmV5ONV3A7BdPNNDG6OscAlZ4-KY1LRciZKTB_fOx8Vz1K6wphTJcTT4qikXEpcyePi79INvelQ75ILdr0bzym7HlnXdQmdLr__XKR3yIe1r31G6bcPs3XsfVihxVK9R5c3QGRs9tc-7z4ggxqXQdOHEdNCIQ4otgiqvvOriDYmexdyeovMNscuruI2QfvOhGh32SWUBxPSBr4zIGNArm29BYrdPS-etKZL7sX0Pil-ffl8ufg2u_jx9XxxdjGzvKJ51lKBG1wqwxxmlSS4lbUinOMabGK14o4rWgnCDbhhnGTENI5SQkXpOJeYnhSv97qbLiY9GZ00oRRXhGFZAeJ8j2iiudKbwfdm2OlovL65iMNKmyF72zktmoa3qmqqUjomVaMEM6wqrWIKN9zWoPVp6rate9dYMGcw3YHoYSX4tV7Fa00FUYxIEDidBIb4Z-tS1r1P428xwYG5MHcpJKSjHOd-8x_04e0m1MrAAj60EfraUVSfwQqMVJIKQM0fQMHTuN5bSGXr4f6AwPYEO8SUBtfe7UiwHjN9O4weM62nTAPt1X1_7ki3Iab_AIJM9Qk</recordid><startdate>20130405</startdate><enddate>20130405</enddate><creator>Zhou, Miao-ni</creator><creator>Zhang, Zhi-qing</creator><creator>Wu, Ji-long</creator><creator>Lin, Fu-quan</creator><creator>Fu, Li-fang</creator><creator>Wang, Sui-qan</creator><creator>Guan, Cui-ping</creator><creator>Wang, Hong-lin</creator><creator>Xu, Aie</creator><general>Public Library of Science</general><general>Public Library of Science (PLoS)</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7QG</scope><scope>7QL</scope><scope>7QO</scope><scope>7RV</scope><scope>7SN</scope><scope>7SS</scope><scope>7T5</scope><scope>7TG</scope><scope>7TM</scope><scope>7U9</scope><scope>7X2</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>8AO</scope><scope>8C1</scope><scope>8FD</scope><scope>8FE</scope><scope>8FG</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABJCF</scope><scope>ABUWG</scope><scope>AEUYN</scope><scope>AFKRA</scope><scope>ARAPS</scope><scope>ATCPS</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BGLVJ</scope><scope>BHPHI</scope><scope>C1K</scope><scope>CCPQU</scope><scope>D1I</scope><scope>DWQXO</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>H94</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>KB.</scope><scope>KB0</scope><scope>KL.</scope><scope>L6V</scope><scope>LK8</scope><scope>M0K</scope><scope>M0S</scope><scope>M1P</scope><scope>M7N</scope><scope>M7P</scope><scope>M7S</scope><scope>NAPCQ</scope><scope>P5Z</scope><scope>P62</scope><scope>P64</scope><scope>PATMY</scope><scope>PDBOC</scope><scope>PIMPY</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>PTHSS</scope><scope>PYCSY</scope><scope>RC3</scope><scope>7X8</scope><scope>5PM</scope><scope>DOA</scope></search><sort><creationdate>20130405</creationdate><title>Dermal mesenchymal stem cells (DMSCs) inhibit skin-homing CD8+ T cell activity, a determining factor of vitiligo patients' autologous melanocytes transplantation efficiency</title><author>Zhou, Miao-ni ; Zhang, Zhi-qing ; Wu, Ji-long ; Lin, Fu-quan ; Fu, Li-fang ; Wang, Sui-qan ; Guan, Cui-ping ; Wang, Hong-lin ; Xu, Aie</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c593t-f360d028a4e049710f7b81550b0064b85e5839615a866ae741ade331362e55703</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2013</creationdate><topic>Adult</topic><topic>Apoptosis</topic><topic>Autografts</topic><topic>Biology</topic><topic>Bone marrow</topic><topic>CD8 antigen</topic><topic>CD8-Positive T-Lymphocytes - immunology</topic><topic>CD8-Positive T-Lymphocytes - pathology</topic><topic>Cell culture</topic><topic>Cell growth</topic><topic>Cell Proliferation</topic><topic>Cell Transplantation</topic><topic>Chemokines</topic><topic>Chemokines - biosynthesis</topic><topic>Chemokines - metabolism</topic><topic>Cytokines</topic><topic>Cytotoxicity</topic><topic>Dermatology</topic><topic>Efficiency</topic><topic>Epidermis - immunology</topic><topic>Epidermis - metabolism</topic><topic>Epidermis - pathology</topic><topic>Female</topic><topic>Gene Expression Regulation - immunology</topic><topic>Homing</topic><topic>Hospitals</topic><topic>Humans</topic><topic>Lymphocytes</topic><topic>Lymphocytes T</topic><topic>Male</topic><topic>Medical research</topic><topic>Medicine</topic><topic>Melanocytes</topic><topic>Melanocytes - transplantation</topic><topic>Mesenchymal stem cells</topic><topic>Mesenchymal Stromal Cells - cytology</topic><topic>Mesenchyme</topic><topic>Nitric oxide</topic><topic>Patients</topic><topic>Pigmentation</topic><topic>Pigmentation - 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Out of 23 patients with the autologous melanocyte transplantation, 12 patients (52.17%) had an excellent re-pigmentation, 6 patients (26.09%) had a good re-pigmentation, 5 patients (21.74%) had a fair or poor re-pigmentation. CD8+ T cells infiltrating was observed in the perilesional vitiligo area of all patients. Importantly, the efficiency of the transplantation was closely associated with skin-homing CD8+ T cell activities. The patients with high number of perilesional CD8+ T cells or high level of cytokines/chemokines were associated with poor re-pigmentation efficiency. For in-vitro experiments, we successfully isolated and characterized human DMSCs and skin-homing CD8+ T cells. We established DMSCs and CD8+ T cell co-culture system, where DMSCs possessed significant inhibitory effects against skin homing CD8+ T lymphocytes. DMSCs inhibited CD8+ T cells proliferation, induced them apoptosis and regulated their cytokines/chemokines production. Our results suggest that vitiligo patients' autologous melanocytes transplantation efficiency might be predicted by perilesional skin-homing CD8+ T cell activities, and DMSCs might be used as auxiliary agent to improve transplantation efficacy.</abstract><cop>United States</cop><pub>Public Library of Science</pub><pmid>23577097</pmid><doi>10.1371/journal.pone.0060254</doi><oa>free_for_read</oa></addata></record> |
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| identifier | ISSN: 1932-6203 |
| ispartof | PloS one, 2013-04, Vol.8 (4), p.e60254-e60254 |
| issn | 1932-6203 1932-6203 |
| language | eng |
| recordid | cdi_plos_journals_1330914079 |
| source | MEDLINE; Public Library of Science; DOAJ Directory of Open Access Journals; PubMed Central; Free Full-Text Journals in Chemistry; EZB Electronic Journals Library |
| subjects | Adult Apoptosis Autografts Biology Bone marrow CD8 antigen CD8-Positive T-Lymphocytes - immunology CD8-Positive T-Lymphocytes - pathology Cell culture Cell growth Cell Proliferation Cell Transplantation Chemokines Chemokines - biosynthesis Chemokines - metabolism Cytokines Cytotoxicity Dermatology Efficiency Epidermis - immunology Epidermis - metabolism Epidermis - pathology Female Gene Expression Regulation - immunology Homing Hospitals Humans Lymphocytes Lymphocytes T Male Medical research Medicine Melanocytes Melanocytes - transplantation Mesenchymal stem cells Mesenchymal Stromal Cells - cytology Mesenchyme Nitric oxide Patients Pigmentation Pigmentation - immunology Skin Skin - cytology Skin - immunology Skin - metabolism Skin - pathology Stem cells Studies T cells Transplantation Transplantation, Autologous Transplants & implants Vitiligo Vitiligo - immunology Vitiligo - metabolism Vitiligo - surgery |
| title | Dermal mesenchymal stem cells (DMSCs) inhibit skin-homing CD8+ T cell activity, a determining factor of vitiligo patients' autologous melanocytes transplantation efficiency |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-02-07T19%3A11%3A32IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-gale_plos_&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Dermal%20mesenchymal%20stem%20cells%20(DMSCs)%20inhibit%20skin-homing%20CD8+%20T%20cell%20activity,%20a%20determining%20factor%20of%20vitiligo%20patients'%20autologous%20melanocytes%20transplantation%20efficiency&rft.jtitle=PloS%20one&rft.au=Zhou,%20Miao-ni&rft.date=2013-04-05&rft.volume=8&rft.issue=4&rft.spage=e60254&rft.epage=e60254&rft.pages=e60254-e60254&rft.issn=1932-6203&rft.eissn=1932-6203&rft_id=info:doi/10.1371/journal.pone.0060254&rft_dat=%3Cgale_plos_%3EA478419736%3C/gale_plos_%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=1330914079&rft_id=info:pmid/23577097&rft_galeid=A478419736&rft_doaj_id=oai_doaj_org_article_6dd5f89d927e478d864a492c8480d5cb&rfr_iscdi=true |