Lack of liver X receptors leads to cell proliferation in a model of mouse dorsal prostate epithelial cell
Recent studies underline the implication of Liver X Receptors (LXRs) in several prostate diseases such as benign prostatic hyperplasia (BPH) and prostate cancer. In order to understand the molecular mechanisms involved, we derived epithelial cells from dorsal prostate (MPECs) of wild type (WT) or Lx...
Gespeichert in:
| Veröffentlicht in: | PloS one 2013-03, Vol.8 (3), p.e58876-e58876 |
|---|---|
| Hauptverfasser: | , , , , , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | e58876 |
|---|---|
| container_issue | 3 |
| container_start_page | e58876 |
| container_title | PloS one |
| container_volume | 8 |
| creator | Dufour, Julie Pommier, Aurélien Alves, Georges De Boussac, Hugues Lours-Calet, Corinne Volle, David H Lobaccaro, Jean-Marc A Baron, Silvère |
| description | Recent studies underline the implication of Liver X Receptors (LXRs) in several prostate diseases such as benign prostatic hyperplasia (BPH) and prostate cancer. In order to understand the molecular mechanisms involved, we derived epithelial cells from dorsal prostate (MPECs) of wild type (WT) or Lxrαβ-/- mice. In the WT MPECs, our results show that LXR activation reduces proliferation and correlates with the modification of the AKT-survival pathway. Moreover, LXRs regulate lipid homeostasis with the regulation of Abca1, Abcg1 and Idol, and, in a lesser extent, Srebp1, Fas and Acc. Conversely cells derived from Lxrαβ-/- mice show a higher basal phosphorylation and consequently activation of the survival/proliferation transduction pathways AKT and MAPK. Altogether, our data point out that the cell model we developed allows deciphering the molecular mechanisms inducing the cell cycle arrest. Besides, we show that activated LXRs regulate AKT and MAPK transduction pathways and demonstrate that LXRs could be good pharmacological targets in prostate disease such as cancer. |
| doi_str_mv | 10.1371/journal.pone.0058876 |
| format | Article |
| fullrecord | <record><control><sourceid>gale_plos_</sourceid><recordid>TN_cdi_plos_journals_1330898395</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><galeid>A478301015</galeid><doaj_id>oai_doaj_org_article_0259e1cb4484432bbe8117343c1bab1d</doaj_id><sourcerecordid>A478301015</sourcerecordid><originalsourceid>FETCH-LOGICAL-c692t-c687101212f9b97f50c2b76d8c1f76c5cf6445047a7adf4faab81407be916ae13</originalsourceid><addsrcrecordid>eNqNk12L1DAUhoso7jr6D0QDgujFjEmTtumNsCx-DAws-IV3IU1PZrJmmm6SLvrvTXe6y1T2QgptOX3eN82bc7LsOcErQivy7tINvpN21bsOVhgXnFflg-yU1DRfljmmD4_eT7InIVwmiPKyfJyd5LQoWM2q08xspPqFnEbWXINHP5EHBX10PiALsg0oOqTAWtR7Z40GL6NxHTIdkmjvWrCjdu-GAKhNInkDhigjIOhN3IE1qTY6PM0eaWkDPJuei-z7xw_fzj8vNxef1udnm6Uq6zymO68IJjnJdd3UlS6wypuqbLkiuipVoXTJWIFZJSvZaqalbDhhuGqgJqUEQhfZy4Nvb10QU0pBEEoxrzmti0SsD0Tr5KXovdlL_0c4acRNwfmtkD4aZUHgvKiBqIYxzhjNmwY4IRVlVJFGNqRNXu-n1YZmD62CLnppZ6bzL53Zia27FrSoizxZLbI3k4F3VwOEKPYmjHnJDlKs6b9zlk6N1zShr_5B79_dRG1l2oDptEvrqtFUnLGKU5zSHanVPVS6WtgblVpKm1SfCd7OBImJ8Dtu5RCCWH_98v_sxY85-_qI3YG0cRecHcY2C3OQHUCVGix40HchEyzGibhNQ4wTIaaJSLIXxwd0J7odAfoX3NYFmA</addsrcrecordid><sourcetype>Open Website</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>1330898395</pqid></control><display><type>article</type><title>Lack of liver X receptors leads to cell proliferation in a model of mouse dorsal prostate epithelial cell</title><source>MEDLINE</source><source>DOAJ Directory of Open Access Journals</source><source>Public Library of Science (PLoS) Journals Open Access</source><source>EZB-FREE-00999 freely available EZB journals</source><source>PubMed Central</source><source>Free Full-Text Journals in Chemistry</source><creator>Dufour, Julie ; Pommier, Aurélien ; Alves, Georges ; De Boussac, Hugues ; Lours-Calet, Corinne ; Volle, David H ; Lobaccaro, Jean-Marc A ; Baron, Silvère</creator><contributor>Makishima, Makoto</contributor><creatorcontrib>Dufour, Julie ; Pommier, Aurélien ; Alves, Georges ; De Boussac, Hugues ; Lours-Calet, Corinne ; Volle, David H ; Lobaccaro, Jean-Marc A ; Baron, Silvère ; Makishima, Makoto</creatorcontrib><description>Recent studies underline the implication of Liver X Receptors (LXRs) in several prostate diseases such as benign prostatic hyperplasia (BPH) and prostate cancer. In order to understand the molecular mechanisms involved, we derived epithelial cells from dorsal prostate (MPECs) of wild type (WT) or Lxrαβ-/- mice. In the WT MPECs, our results show that LXR activation reduces proliferation and correlates with the modification of the AKT-survival pathway. Moreover, LXRs regulate lipid homeostasis with the regulation of Abca1, Abcg1 and Idol, and, in a lesser extent, Srebp1, Fas and Acc. Conversely cells derived from Lxrαβ-/- mice show a higher basal phosphorylation and consequently activation of the survival/proliferation transduction pathways AKT and MAPK. Altogether, our data point out that the cell model we developed allows deciphering the molecular mechanisms inducing the cell cycle arrest. Besides, we show that activated LXRs regulate AKT and MAPK transduction pathways and demonstrate that LXRs could be good pharmacological targets in prostate disease such as cancer.</description><identifier>ISSN: 1932-6203</identifier><identifier>EISSN: 1932-6203</identifier><identifier>DOI: 10.1371/journal.pone.0058876</identifier><identifier>PMID: 23554947</identifier><language>eng</language><publisher>United States: Public Library of Science</publisher><subject>ABCA1 protein ; Activation ; AKT protein ; Animals ; Apoptosis ; ATP-binding protein ; Biology ; Cancer ; Cell culture ; Cell cycle ; Cell Line ; Cell Proliferation ; Cholesterol ; Epithelial cells ; Epithelial Cells - metabolism ; Extracellular matrix ; Fatty acids ; Gene Order ; Gene Targeting ; Genotype ; Homeostasis ; Homeostasis - genetics ; Hyperplasia ; Kinases ; Ligands ; Lipid Metabolism - genetics ; Liver ; Liver X Receptors ; Male ; MAP kinase ; Medicine ; Mice ; Mice, Knockout ; Mitogen-Activated Protein Kinases - metabolism ; Molecular modelling ; Nutrition ; Orphan Nuclear Receptors - genetics ; Orphan Nuclear Receptors - metabolism ; Pascal, Blaise (1623-1662) ; Pathways ; Pharmacology ; Phosphorylation ; Prostate - metabolism ; Prostate cancer ; Proto-Oncogene Proteins c-akt - metabolism ; Receptors ; Regulation ; Rodents ; Signal Transduction ; Stromal Cells - metabolism ; Studies ; Survival</subject><ispartof>PloS one, 2013-03, Vol.8 (3), p.e58876-e58876</ispartof><rights>COPYRIGHT 2013 Public Library of Science</rights><rights>2013 Dufour et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License: https://creativecommons.org/licenses/by/4.0/ (the “License”), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><rights>2013 Dufour et al 2013 Dufour et al</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c692t-c687101212f9b97f50c2b76d8c1f76c5cf6445047a7adf4faab81407be916ae13</citedby><cites>FETCH-LOGICAL-c692t-c687101212f9b97f50c2b76d8c1f76c5cf6445047a7adf4faab81407be916ae13</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC3595217/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC3595217/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,315,728,781,785,865,886,2103,2929,23871,27929,27930,53796,53798</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/23554947$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><contributor>Makishima, Makoto</contributor><creatorcontrib>Dufour, Julie</creatorcontrib><creatorcontrib>Pommier, Aurélien</creatorcontrib><creatorcontrib>Alves, Georges</creatorcontrib><creatorcontrib>De Boussac, Hugues</creatorcontrib><creatorcontrib>Lours-Calet, Corinne</creatorcontrib><creatorcontrib>Volle, David H</creatorcontrib><creatorcontrib>Lobaccaro, Jean-Marc A</creatorcontrib><creatorcontrib>Baron, Silvère</creatorcontrib><title>Lack of liver X receptors leads to cell proliferation in a model of mouse dorsal prostate epithelial cell</title><title>PloS one</title><addtitle>PLoS One</addtitle><description>Recent studies underline the implication of Liver X Receptors (LXRs) in several prostate diseases such as benign prostatic hyperplasia (BPH) and prostate cancer. In order to understand the molecular mechanisms involved, we derived epithelial cells from dorsal prostate (MPECs) of wild type (WT) or Lxrαβ-/- mice. In the WT MPECs, our results show that LXR activation reduces proliferation and correlates with the modification of the AKT-survival pathway. Moreover, LXRs regulate lipid homeostasis with the regulation of Abca1, Abcg1 and Idol, and, in a lesser extent, Srebp1, Fas and Acc. Conversely cells derived from Lxrαβ-/- mice show a higher basal phosphorylation and consequently activation of the survival/proliferation transduction pathways AKT and MAPK. Altogether, our data point out that the cell model we developed allows deciphering the molecular mechanisms inducing the cell cycle arrest. Besides, we show that activated LXRs regulate AKT and MAPK transduction pathways and demonstrate that LXRs could be good pharmacological targets in prostate disease such as cancer.</description><subject>ABCA1 protein</subject><subject>Activation</subject><subject>AKT protein</subject><subject>Animals</subject><subject>Apoptosis</subject><subject>ATP-binding protein</subject><subject>Biology</subject><subject>Cancer</subject><subject>Cell culture</subject><subject>Cell cycle</subject><subject>Cell Line</subject><subject>Cell Proliferation</subject><subject>Cholesterol</subject><subject>Epithelial cells</subject><subject>Epithelial Cells - metabolism</subject><subject>Extracellular matrix</subject><subject>Fatty acids</subject><subject>Gene Order</subject><subject>Gene Targeting</subject><subject>Genotype</subject><subject>Homeostasis</subject><subject>Homeostasis - genetics</subject><subject>Hyperplasia</subject><subject>Kinases</subject><subject>Ligands</subject><subject>Lipid Metabolism - genetics</subject><subject>Liver</subject><subject>Liver X Receptors</subject><subject>Male</subject><subject>MAP kinase</subject><subject>Medicine</subject><subject>Mice</subject><subject>Mice, Knockout</subject><subject>Mitogen-Activated Protein Kinases - metabolism</subject><subject>Molecular modelling</subject><subject>Nutrition</subject><subject>Orphan Nuclear Receptors - genetics</subject><subject>Orphan Nuclear Receptors - metabolism</subject><subject>Pascal, Blaise (1623-1662)</subject><subject>Pathways</subject><subject>Pharmacology</subject><subject>Phosphorylation</subject><subject>Prostate - metabolism</subject><subject>Prostate cancer</subject><subject>Proto-Oncogene Proteins c-akt - metabolism</subject><subject>Receptors</subject><subject>Regulation</subject><subject>Rodents</subject><subject>Signal Transduction</subject><subject>Stromal Cells - metabolism</subject><subject>Studies</subject><subject>Survival</subject><issn>1932-6203</issn><issn>1932-6203</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2013</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><sourceid>DOA</sourceid><recordid>eNqNk12L1DAUhoso7jr6D0QDgujFjEmTtumNsCx-DAws-IV3IU1PZrJmmm6SLvrvTXe6y1T2QgptOX3eN82bc7LsOcErQivy7tINvpN21bsOVhgXnFflg-yU1DRfljmmD4_eT7InIVwmiPKyfJyd5LQoWM2q08xspPqFnEbWXINHP5EHBX10PiALsg0oOqTAWtR7Z40GL6NxHTIdkmjvWrCjdu-GAKhNInkDhigjIOhN3IE1qTY6PM0eaWkDPJuei-z7xw_fzj8vNxef1udnm6Uq6zymO68IJjnJdd3UlS6wypuqbLkiuipVoXTJWIFZJSvZaqalbDhhuGqgJqUEQhfZy4Nvb10QU0pBEEoxrzmti0SsD0Tr5KXovdlL_0c4acRNwfmtkD4aZUHgvKiBqIYxzhjNmwY4IRVlVJFGNqRNXu-n1YZmD62CLnppZ6bzL53Zia27FrSoizxZLbI3k4F3VwOEKPYmjHnJDlKs6b9zlk6N1zShr_5B79_dRG1l2oDptEvrqtFUnLGKU5zSHanVPVS6WtgblVpKm1SfCd7OBImJ8Dtu5RCCWH_98v_sxY85-_qI3YG0cRecHcY2C3OQHUCVGix40HchEyzGibhNQ4wTIaaJSLIXxwd0J7odAfoX3NYFmA</recordid><startdate>20130312</startdate><enddate>20130312</enddate><creator>Dufour, Julie</creator><creator>Pommier, Aurélien</creator><creator>Alves, Georges</creator><creator>De Boussac, Hugues</creator><creator>Lours-Calet, Corinne</creator><creator>Volle, David H</creator><creator>Lobaccaro, Jean-Marc A</creator><creator>Baron, Silvère</creator><general>Public Library of Science</general><general>Public Library of Science (PLoS)</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>IOV</scope><scope>ISR</scope><scope>3V.</scope><scope>7QG</scope><scope>7QL</scope><scope>7QO</scope><scope>7RV</scope><scope>7SN</scope><scope>7SS</scope><scope>7T5</scope><scope>7TG</scope><scope>7TM</scope><scope>7U9</scope><scope>7X2</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>8AO</scope><scope>8C1</scope><scope>8FD</scope><scope>8FE</scope><scope>8FG</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABJCF</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>ARAPS</scope><scope>ATCPS</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BGLVJ</scope><scope>BHPHI</scope><scope>C1K</scope><scope>CCPQU</scope><scope>D1I</scope><scope>DWQXO</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>H94</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>KB.</scope><scope>KB0</scope><scope>KL.</scope><scope>L6V</scope><scope>LK8</scope><scope>M0K</scope><scope>M0S</scope><scope>M1P</scope><scope>M7N</scope><scope>M7P</scope><scope>M7S</scope><scope>NAPCQ</scope><scope>P5Z</scope><scope>P62</scope><scope>P64</scope><scope>PATMY</scope><scope>PDBOC</scope><scope>PIMPY</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PTHSS</scope><scope>PYCSY</scope><scope>RC3</scope><scope>7X8</scope><scope>5PM</scope><scope>DOA</scope></search><sort><creationdate>20130312</creationdate><title>Lack of liver X receptors leads to cell proliferation in a model of mouse dorsal prostate epithelial cell</title><author>Dufour, Julie ; Pommier, Aurélien ; Alves, Georges ; De Boussac, Hugues ; Lours-Calet, Corinne ; Volle, David H ; Lobaccaro, Jean-Marc A ; Baron, Silvère</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c692t-c687101212f9b97f50c2b76d8c1f76c5cf6445047a7adf4faab81407be916ae13</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2013</creationdate><topic>ABCA1 protein</topic><topic>Activation</topic><topic>AKT protein</topic><topic>Animals</topic><topic>Apoptosis</topic><topic>ATP-binding protein</topic><topic>Biology</topic><topic>Cancer</topic><topic>Cell culture</topic><topic>Cell cycle</topic><topic>Cell Line</topic><topic>Cell Proliferation</topic><topic>Cholesterol</topic><topic>Epithelial cells</topic><topic>Epithelial Cells - metabolism</topic><topic>Extracellular matrix</topic><topic>Fatty acids</topic><topic>Gene Order</topic><topic>Gene Targeting</topic><topic>Genotype</topic><topic>Homeostasis</topic><topic>Homeostasis - genetics</topic><topic>Hyperplasia</topic><topic>Kinases</topic><topic>Ligands</topic><topic>Lipid Metabolism - genetics</topic><topic>Liver</topic><topic>Liver X Receptors</topic><topic>Male</topic><topic>MAP kinase</topic><topic>Medicine</topic><topic>Mice</topic><topic>Mice, Knockout</topic><topic>Mitogen-Activated Protein Kinases - metabolism</topic><topic>Molecular modelling</topic><topic>Nutrition</topic><topic>Orphan Nuclear Receptors - genetics</topic><topic>Orphan Nuclear Receptors - metabolism</topic><topic>Pascal, Blaise (1623-1662)</topic><topic>Pathways</topic><topic>Pharmacology</topic><topic>Phosphorylation</topic><topic>Prostate - metabolism</topic><topic>Prostate cancer</topic><topic>Proto-Oncogene Proteins c-akt - metabolism</topic><topic>Receptors</topic><topic>Regulation</topic><topic>Rodents</topic><topic>Signal Transduction</topic><topic>Stromal Cells - metabolism</topic><topic>Studies</topic><topic>Survival</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Dufour, Julie</creatorcontrib><creatorcontrib>Pommier, Aurélien</creatorcontrib><creatorcontrib>Alves, Georges</creatorcontrib><creatorcontrib>De Boussac, Hugues</creatorcontrib><creatorcontrib>Lours-Calet, Corinne</creatorcontrib><creatorcontrib>Volle, David H</creatorcontrib><creatorcontrib>Lobaccaro, Jean-Marc A</creatorcontrib><creatorcontrib>Baron, Silvère</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Gale In Context: Opposing Viewpoints</collection><collection>Gale In Context: Science</collection><collection>ProQuest Central (Corporate)</collection><collection>Animal Behavior Abstracts</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Biotechnology Research Abstracts</collection><collection>Nursing & Allied Health Database</collection><collection>Ecology Abstracts</collection><collection>Entomology Abstracts (Full archive)</collection><collection>Immunology Abstracts</collection><collection>Meteorological & Geoastrophysical Abstracts</collection><collection>Nucleic Acids Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>Agricultural Science Collection</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Medical Database (Alumni Edition)</collection><collection>ProQuest Pharma Collection</collection><collection>Public Health Database</collection><collection>Technology Research Database</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Technology Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>Materials Science & Engineering Collection</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest Central UK/Ireland</collection><collection>Advanced Technologies & Aerospace Collection</collection><collection>Agricultural & Environmental Science Collection</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Technology Collection</collection><collection>Natural Science Collection (ProQuest)</collection><collection>Environmental Sciences and Pollution Management</collection><collection>ProQuest One Community College</collection><collection>ProQuest Materials Science Collection</collection><collection>ProQuest Central Korea</collection><collection>Engineering Research Database</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Materials Science Database</collection><collection>Nursing & Allied Health Database (Alumni Edition)</collection><collection>Meteorological & Geoastrophysical Abstracts - Academic</collection><collection>ProQuest Engineering Collection</collection><collection>ProQuest Biological Science Collection</collection><collection>Agricultural Science Database</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biological Science Database</collection><collection>Engineering Database</collection><collection>Nursing & Allied Health Premium</collection><collection>Advanced Technologies & Aerospace Database</collection><collection>ProQuest Advanced Technologies & Aerospace Collection</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Environmental Science Database</collection><collection>Materials Science Collection</collection><collection>Access via ProQuest (Open Access)</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>Engineering Collection</collection><collection>Environmental Science Collection</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><collection>DOAJ Directory of Open Access Journals</collection><jtitle>PloS one</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Dufour, Julie</au><au>Pommier, Aurélien</au><au>Alves, Georges</au><au>De Boussac, Hugues</au><au>Lours-Calet, Corinne</au><au>Volle, David H</au><au>Lobaccaro, Jean-Marc A</au><au>Baron, Silvère</au><au>Makishima, Makoto</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Lack of liver X receptors leads to cell proliferation in a model of mouse dorsal prostate epithelial cell</atitle><jtitle>PloS one</jtitle><addtitle>PLoS One</addtitle><date>2013-03-12</date><risdate>2013</risdate><volume>8</volume><issue>3</issue><spage>e58876</spage><epage>e58876</epage><pages>e58876-e58876</pages><issn>1932-6203</issn><eissn>1932-6203</eissn><abstract>Recent studies underline the implication of Liver X Receptors (LXRs) in several prostate diseases such as benign prostatic hyperplasia (BPH) and prostate cancer. In order to understand the molecular mechanisms involved, we derived epithelial cells from dorsal prostate (MPECs) of wild type (WT) or Lxrαβ-/- mice. In the WT MPECs, our results show that LXR activation reduces proliferation and correlates with the modification of the AKT-survival pathway. Moreover, LXRs regulate lipid homeostasis with the regulation of Abca1, Abcg1 and Idol, and, in a lesser extent, Srebp1, Fas and Acc. Conversely cells derived from Lxrαβ-/- mice show a higher basal phosphorylation and consequently activation of the survival/proliferation transduction pathways AKT and MAPK. Altogether, our data point out that the cell model we developed allows deciphering the molecular mechanisms inducing the cell cycle arrest. Besides, we show that activated LXRs regulate AKT and MAPK transduction pathways and demonstrate that LXRs could be good pharmacological targets in prostate disease such as cancer.</abstract><cop>United States</cop><pub>Public Library of Science</pub><pmid>23554947</pmid><doi>10.1371/journal.pone.0058876</doi><tpages>e58876</tpages><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 1932-6203 |
| ispartof | PloS one, 2013-03, Vol.8 (3), p.e58876-e58876 |
| issn | 1932-6203 1932-6203 |
| language | eng |
| recordid | cdi_plos_journals_1330898395 |
| source | MEDLINE; DOAJ Directory of Open Access Journals; Public Library of Science (PLoS) Journals Open Access; EZB-FREE-00999 freely available EZB journals; PubMed Central; Free Full-Text Journals in Chemistry |
| subjects | ABCA1 protein Activation AKT protein Animals Apoptosis ATP-binding protein Biology Cancer Cell culture Cell cycle Cell Line Cell Proliferation Cholesterol Epithelial cells Epithelial Cells - metabolism Extracellular matrix Fatty acids Gene Order Gene Targeting Genotype Homeostasis Homeostasis - genetics Hyperplasia Kinases Ligands Lipid Metabolism - genetics Liver Liver X Receptors Male MAP kinase Medicine Mice Mice, Knockout Mitogen-Activated Protein Kinases - metabolism Molecular modelling Nutrition Orphan Nuclear Receptors - genetics Orphan Nuclear Receptors - metabolism Pascal, Blaise (1623-1662) Pathways Pharmacology Phosphorylation Prostate - metabolism Prostate cancer Proto-Oncogene Proteins c-akt - metabolism Receptors Regulation Rodents Signal Transduction Stromal Cells - metabolism Studies Survival |
| title | Lack of liver X receptors leads to cell proliferation in a model of mouse dorsal prostate epithelial cell |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2024-12-11T16%3A19%3A47IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-gale_plos_&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Lack%20of%20liver%20X%20receptors%20leads%20to%20cell%20proliferation%20in%20a%20model%20of%20mouse%20dorsal%20prostate%20epithelial%20cell&rft.jtitle=PloS%20one&rft.au=Dufour,%20Julie&rft.date=2013-03-12&rft.volume=8&rft.issue=3&rft.spage=e58876&rft.epage=e58876&rft.pages=e58876-e58876&rft.issn=1932-6203&rft.eissn=1932-6203&rft_id=info:doi/10.1371/journal.pone.0058876&rft_dat=%3Cgale_plos_%3EA478301015%3C/gale_plos_%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=1330898395&rft_id=info:pmid/23554947&rft_galeid=A478301015&rft_doaj_id=oai_doaj_org_article_0259e1cb4484432bbe8117343c1bab1d&rfr_iscdi=true |