Generation of live offspring from vitrified mouse oocytes of C57BL/6J strain
In mammals, unfertilized oocytes are one of the most available stages for cryopreservation because the cryopreserved oocytes can be used for assisted reproductive technologies, including in vitro fertilization (IVF) and intracytoplasmic sperm injection. However, it has generally been reported that t...
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| creator | Kohaya, Natsuki Fujiwara, Katsuyoshi Ito, Junya Kashiwazaki, Naomi |
| description | In mammals, unfertilized oocytes are one of the most available stages for cryopreservation because the cryopreserved oocytes can be used for assisted reproductive technologies, including in vitro fertilization (IVF) and intracytoplasmic sperm injection. However, it has generally been reported that the fertility and developmental ability of the oocytes are reduced by cryopreservation. C57BL/6J mice, an inbred strain, are used extensively for the production of transgenic and knockout mice. If the oocytes from C57BL/6J mice can be successfully cryopreserved, the cryopreservation protocol used will contribute to the high-speed production of not only gene-modified mice but also hybrid mice. Very recently, we succeeded in the vitrification of mouse oocytes derived from ICR (outbred) mice. However, our protocol can be applied to the vitrification of oocytes from an inbred strain. The aim of the present study was to establish the vitrification of oocytes from C57BL/6J mice. First, the effect of cumulus cells on the ability of C57BL/6J mouse oocytes to fertilize and develop in vitro was examined. The fertility and developmental ability of oocyte-removed cumulus cells (i.e., denuded oocytes, or DOs) after IVF were reduced compared to cumulus oocyte complexes (COCs) in both fresh and cryopreserved groups. Vitrified COCs showed significantly (P |
| doi_str_mv | 10.1371/journal.pone.0058063 |
| format | Article |
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However, it has generally been reported that the fertility and developmental ability of the oocytes are reduced by cryopreservation. C57BL/6J mice, an inbred strain, are used extensively for the production of transgenic and knockout mice. If the oocytes from C57BL/6J mice can be successfully cryopreserved, the cryopreservation protocol used will contribute to the high-speed production of not only gene-modified mice but also hybrid mice. Very recently, we succeeded in the vitrification of mouse oocytes derived from ICR (outbred) mice. However, our protocol can be applied to the vitrification of oocytes from an inbred strain. The aim of the present study was to establish the vitrification of oocytes from C57BL/6J mice. First, the effect of cumulus cells on the ability of C57BL/6J mouse oocytes to fertilize and develop in vitro was examined. The fertility and developmental ability of oocyte-removed cumulus cells (i.e., denuded oocytes, or DOs) after IVF were reduced compared to cumulus oocyte complexes (COCs) in both fresh and cryopreserved groups. Vitrified COCs showed significantly (P<0.05) higher fertility and ability to develop into the 2-cell and blastocyst stages compared to the vitrified DOs with cumulus cells and vitrified DOs alone. The vitrified COCs developed to term at a high success rate, equivalent to the rate obtained with IVF using fresh COCs. Taken together, our results demonstrate that we succeeded for the first time in the vitrification of mouse oocytes from C57BL/6J mice. Our findings will also contribute to the improvement of oocyte vitrification not only in animals but also in clinical applications for human infertility.</description><identifier>ISSN: 1932-6203</identifier><identifier>EISSN: 1932-6203</identifier><identifier>DOI: 10.1371/journal.pone.0058063</identifier><identifier>PMID: 23516430</identifier><language>eng</language><publisher>United States: Public Library of Science</publisher><subject>Agriculture ; Animal reproduction ; Animals ; Biology ; Cooling ; Cryopreservation ; Cumulus Cells ; Deoxyribonucleic acid ; DNA ; Embryo Transfer ; Experiments ; Female ; Females ; Fertility ; Fertilization in Vitro ; Gene expression ; Genetic engineering ; House mouse ; In vitro fertilization ; Inbreeding ; Infertility ; Laboratories ; Male ; Mammals ; Medicine ; Mice ; Mice, Inbred C57BL ; Offspring ; Oocytes ; Reproductive technologies ; Reproductive technology ; Rodents ; Sperm ; Therapeutic applications ; Transgenic mice ; Veterinary colleges ; Veterinary medicine ; Vitrification</subject><ispartof>PloS one, 2013-03, Vol.8 (3), p.e58063-e58063</ispartof><rights>COPYRIGHT 2013 Public Library of Science</rights><rights>2013 Kohaya et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License: https://creativecommons.org/licenses/by/4.0/ (the “License”), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><rights>2013 Kohaya et al 2013 Kohaya et al</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c758t-bc45b29e7c8726a82e899a276cbf90f79c5ff57c1066a24075928504ca431d623</citedby><cites>FETCH-LOGICAL-c758t-bc45b29e7c8726a82e899a276cbf90f79c5ff57c1066a24075928504ca431d623</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC3596345/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC3596345/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,727,780,784,864,885,2102,2928,23866,27924,27925,53791,53793,79600,79601</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/23516430$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><contributor>Herault, Yann</contributor><creatorcontrib>Kohaya, Natsuki</creatorcontrib><creatorcontrib>Fujiwara, Katsuyoshi</creatorcontrib><creatorcontrib>Ito, Junya</creatorcontrib><creatorcontrib>Kashiwazaki, Naomi</creatorcontrib><title>Generation of live offspring from vitrified mouse oocytes of C57BL/6J strain</title><title>PloS one</title><addtitle>PLoS One</addtitle><description>In mammals, unfertilized oocytes are one of the most available stages for cryopreservation because the cryopreserved oocytes can be used for assisted reproductive technologies, including in vitro fertilization (IVF) and intracytoplasmic sperm injection. However, it has generally been reported that the fertility and developmental ability of the oocytes are reduced by cryopreservation. C57BL/6J mice, an inbred strain, are used extensively for the production of transgenic and knockout mice. If the oocytes from C57BL/6J mice can be successfully cryopreserved, the cryopreservation protocol used will contribute to the high-speed production of not only gene-modified mice but also hybrid mice. Very recently, we succeeded in the vitrification of mouse oocytes derived from ICR (outbred) mice. However, our protocol can be applied to the vitrification of oocytes from an inbred strain. The aim of the present study was to establish the vitrification of oocytes from C57BL/6J mice. First, the effect of cumulus cells on the ability of C57BL/6J mouse oocytes to fertilize and develop in vitro was examined. The fertility and developmental ability of oocyte-removed cumulus cells (i.e., denuded oocytes, or DOs) after IVF were reduced compared to cumulus oocyte complexes (COCs) in both fresh and cryopreserved groups. Vitrified COCs showed significantly (P<0.05) higher fertility and ability to develop into the 2-cell and blastocyst stages compared to the vitrified DOs with cumulus cells and vitrified DOs alone. The vitrified COCs developed to term at a high success rate, equivalent to the rate obtained with IVF using fresh COCs. Taken together, our results demonstrate that we succeeded for the first time in the vitrification of mouse oocytes from C57BL/6J mice. Our findings will also contribute to the improvement of oocyte vitrification not only in animals but also in clinical applications for human infertility.</description><subject>Agriculture</subject><subject>Animal reproduction</subject><subject>Animals</subject><subject>Biology</subject><subject>Cooling</subject><subject>Cryopreservation</subject><subject>Cumulus Cells</subject><subject>Deoxyribonucleic acid</subject><subject>DNA</subject><subject>Embryo Transfer</subject><subject>Experiments</subject><subject>Female</subject><subject>Females</subject><subject>Fertility</subject><subject>Fertilization in Vitro</subject><subject>Gene expression</subject><subject>Genetic engineering</subject><subject>House mouse</subject><subject>In vitro fertilization</subject><subject>Inbreeding</subject><subject>Infertility</subject><subject>Laboratories</subject><subject>Male</subject><subject>Mammals</subject><subject>Medicine</subject><subject>Mice</subject><subject>Mice, Inbred C57BL</subject><subject>Offspring</subject><subject>Oocytes</subject><subject>Reproductive technologies</subject><subject>Reproductive technology</subject><subject>Rodents</subject><subject>Sperm</subject><subject>Therapeutic applications</subject><subject>Transgenic mice</subject><subject>Veterinary colleges</subject><subject>Veterinary 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One</addtitle><date>2013-03-13</date><risdate>2013</risdate><volume>8</volume><issue>3</issue><spage>e58063</spage><epage>e58063</epage><pages>e58063-e58063</pages><issn>1932-6203</issn><eissn>1932-6203</eissn><abstract>In mammals, unfertilized oocytes are one of the most available stages for cryopreservation because the cryopreserved oocytes can be used for assisted reproductive technologies, including in vitro fertilization (IVF) and intracytoplasmic sperm injection. However, it has generally been reported that the fertility and developmental ability of the oocytes are reduced by cryopreservation. C57BL/6J mice, an inbred strain, are used extensively for the production of transgenic and knockout mice. If the oocytes from C57BL/6J mice can be successfully cryopreserved, the cryopreservation protocol used will contribute to the high-speed production of not only gene-modified mice but also hybrid mice. Very recently, we succeeded in the vitrification of mouse oocytes derived from ICR (outbred) mice. However, our protocol can be applied to the vitrification of oocytes from an inbred strain. The aim of the present study was to establish the vitrification of oocytes from C57BL/6J mice. First, the effect of cumulus cells on the ability of C57BL/6J mouse oocytes to fertilize and develop in vitro was examined. The fertility and developmental ability of oocyte-removed cumulus cells (i.e., denuded oocytes, or DOs) after IVF were reduced compared to cumulus oocyte complexes (COCs) in both fresh and cryopreserved groups. Vitrified COCs showed significantly (P<0.05) higher fertility and ability to develop into the 2-cell and blastocyst stages compared to the vitrified DOs with cumulus cells and vitrified DOs alone. The vitrified COCs developed to term at a high success rate, equivalent to the rate obtained with IVF using fresh COCs. Taken together, our results demonstrate that we succeeded for the first time in the vitrification of mouse oocytes from C57BL/6J mice. Our findings will also contribute to the improvement of oocyte vitrification not only in animals but also in clinical applications for human infertility.</abstract><cop>United States</cop><pub>Public Library of Science</pub><pmid>23516430</pmid><doi>10.1371/journal.pone.0058063</doi><tpages>e58063</tpages><oa>free_for_read</oa></addata></record> |
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| source | MEDLINE; DOAJ Directory of Open Access Journals; Public Library of Science (PLoS) Journals Open Access; EZB-FREE-00999 freely available EZB journals; PubMed Central; Free Full-Text Journals in Chemistry |
| subjects | Agriculture Animal reproduction Animals Biology Cooling Cryopreservation Cumulus Cells Deoxyribonucleic acid DNA Embryo Transfer Experiments Female Females Fertility Fertilization in Vitro Gene expression Genetic engineering House mouse In vitro fertilization Inbreeding Infertility Laboratories Male Mammals Medicine Mice Mice, Inbred C57BL Offspring Oocytes Reproductive technologies Reproductive technology Rodents Sperm Therapeutic applications Transgenic mice Veterinary colleges Veterinary medicine Vitrification |
| title | Generation of live offspring from vitrified mouse oocytes of C57BL/6J strain |
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