Establishment of immortalized human erythroid progenitor cell lines able to produce enucleated red blood cells
Transfusion of red blood cells (RBCs) is a standard and indispensable therapy in current clinical practice. In vitro production of RBCs offers a potential means to overcome a shortage of transfusable RBCs in some clinical situations and also to provide a source of cells free from possible infection...
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| Veröffentlicht in: | PloS one 2013-03, Vol.8 (3), p.e59890-e59890 |
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| creator | Kurita, Ryo Suda, Noriko Sudo, Kazuhiro Miharada, Kenichi Hiroyama, Takashi Miyoshi, Hiroyuki Tani, Kenzaburo Nakamura, Yukio |
| description | Transfusion of red blood cells (RBCs) is a standard and indispensable therapy in current clinical practice. In vitro production of RBCs offers a potential means to overcome a shortage of transfusable RBCs in some clinical situations and also to provide a source of cells free from possible infection or contamination by microorganisms. Thus, in vitro production of RBCs may become a standard procedure in the future. We previously reported the successful establishment of immortalized mouse erythroid progenitor cell lines that were able to produce mature RBCs very efficiently. Here, we have developed a reliable protocol for establishing immortalized human erythroid progenitor cell lines that are able to produce enucleated RBCs. These immortalized cell lines produce functional hemoglobin and express erythroid-specific markers, and these markers are upregulated following induction of differentiation in vitro. Most importantly, these immortalized cell lines all produce enucleated RBCs after induction of differentiation in vitro, although the efficiency of producing enucleated RBCs remains to be improved further. To the best of our knowledge, this is the first demonstration of the feasibility of using immortalized human erythroid progenitor cell lines as an ex vivo source for production of enucleated RBCs. |
| doi_str_mv | 10.1371/journal.pone.0059890 |
| format | Article |
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In vitro production of RBCs offers a potential means to overcome a shortage of transfusable RBCs in some clinical situations and also to provide a source of cells free from possible infection or contamination by microorganisms. Thus, in vitro production of RBCs may become a standard procedure in the future. We previously reported the successful establishment of immortalized mouse erythroid progenitor cell lines that were able to produce mature RBCs very efficiently. Here, we have developed a reliable protocol for establishing immortalized human erythroid progenitor cell lines that are able to produce enucleated RBCs. These immortalized cell lines produce functional hemoglobin and express erythroid-specific markers, and these markers are upregulated following induction of differentiation in vitro. Most importantly, these immortalized cell lines all produce enucleated RBCs after induction of differentiation in vitro, although the efficiency of producing enucleated RBCs remains to be improved further. To the best of our knowledge, this is the first demonstration of the feasibility of using immortalized human erythroid progenitor cell lines as an ex vivo source for production of enucleated RBCs.</description><identifier>ISSN: 1932-6203</identifier><identifier>EISSN: 1932-6203</identifier><identifier>DOI: 10.1371/journal.pone.0059890</identifier><identifier>PMID: 23533656</identifier><language>eng</language><publisher>United States: Public Library of Science</publisher><subject>Biology ; Biotechnology ; Blood ; Blood cells ; Blood transfusion ; Cell lines ; Cell Nucleus ; Contamination ; Deoxyribonucleic acid ; Differentiation ; DNA ; Engineering ; Erythrocyte Transfusion - methods ; Erythrocytes ; Erythrocytes - cytology ; Erythroid Precursor Cells - cytology ; Erythropoiesis - physiology ; Feasibility studies ; Gene expression ; Genetic engineering ; Health aspects ; Hemoglobin ; Hemoglobins ; Humans ; Insulin-like growth factors ; Markers ; Medicine ; Microorganisms ; Progenitor cells ; Rodents ; Stem cells ; Transfusion ; Vascular endothelial growth factor</subject><ispartof>PloS one, 2013-03, Vol.8 (3), p.e59890-e59890</ispartof><rights>COPYRIGHT 2013 Public Library of Science</rights><rights>2013 Kurita et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License: https://creativecommons.org/licenses/by/4.0/ (the “License”), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. 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In vitro production of RBCs offers a potential means to overcome a shortage of transfusable RBCs in some clinical situations and also to provide a source of cells free from possible infection or contamination by microorganisms. Thus, in vitro production of RBCs may become a standard procedure in the future. We previously reported the successful establishment of immortalized mouse erythroid progenitor cell lines that were able to produce mature RBCs very efficiently. Here, we have developed a reliable protocol for establishing immortalized human erythroid progenitor cell lines that are able to produce enucleated RBCs. These immortalized cell lines produce functional hemoglobin and express erythroid-specific markers, and these markers are upregulated following induction of differentiation in vitro. Most importantly, these immortalized cell lines all produce enucleated RBCs after induction of differentiation in vitro, although the efficiency of producing enucleated RBCs remains to be improved further. To the best of our knowledge, this is the first demonstration of the feasibility of using immortalized human erythroid progenitor cell lines as an ex vivo source for production of enucleated RBCs.</description><subject>Biology</subject><subject>Biotechnology</subject><subject>Blood</subject><subject>Blood cells</subject><subject>Blood transfusion</subject><subject>Cell lines</subject><subject>Cell Nucleus</subject><subject>Contamination</subject><subject>Deoxyribonucleic acid</subject><subject>Differentiation</subject><subject>DNA</subject><subject>Engineering</subject><subject>Erythrocyte Transfusion - methods</subject><subject>Erythrocytes</subject><subject>Erythrocytes - cytology</subject><subject>Erythroid Precursor Cells - cytology</subject><subject>Erythropoiesis - physiology</subject><subject>Feasibility studies</subject><subject>Gene expression</subject><subject>Genetic engineering</subject><subject>Health 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In vitro production of RBCs offers a potential means to overcome a shortage of transfusable RBCs in some clinical situations and also to provide a source of cells free from possible infection or contamination by microorganisms. Thus, in vitro production of RBCs may become a standard procedure in the future. We previously reported the successful establishment of immortalized mouse erythroid progenitor cell lines that were able to produce mature RBCs very efficiently. Here, we have developed a reliable protocol for establishing immortalized human erythroid progenitor cell lines that are able to produce enucleated RBCs. These immortalized cell lines produce functional hemoglobin and express erythroid-specific markers, and these markers are upregulated following induction of differentiation in vitro. Most importantly, these immortalized cell lines all produce enucleated RBCs after induction of differentiation in vitro, although the efficiency of producing enucleated RBCs remains to be improved further. To the best of our knowledge, this is the first demonstration of the feasibility of using immortalized human erythroid progenitor cell lines as an ex vivo source for production of enucleated RBCs.</abstract><cop>United States</cop><pub>Public Library of Science</pub><pmid>23533656</pmid><doi>10.1371/journal.pone.0059890</doi><tpages>e59890</tpages><oa>free_for_read</oa></addata></record> |
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| source | Public Library of Science (PLoS) Journals Open Access; MEDLINE; DOAJ Directory of Open Access Journals; EZB-FREE-00999 freely available EZB journals; PubMed Central; Free Full-Text Journals in Chemistry |
| subjects | Biology Biotechnology Blood Blood cells Blood transfusion Cell lines Cell Nucleus Contamination Deoxyribonucleic acid Differentiation DNA Engineering Erythrocyte Transfusion - methods Erythrocytes Erythrocytes - cytology Erythroid Precursor Cells - cytology Erythropoiesis - physiology Feasibility studies Gene expression Genetic engineering Health aspects Hemoglobin Hemoglobins Humans Insulin-like growth factors Markers Medicine Microorganisms Progenitor cells Rodents Stem cells Transfusion Vascular endothelial growth factor |
| title | Establishment of immortalized human erythroid progenitor cell lines able to produce enucleated red blood cells |
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