Pre-existing immunity with high neutralizing activity to 2009 pandemic H1N1 influenza virus in Shanghai population
Pre-existing immunity is an important factor countering the pandemic potential of an emerging influenza virus strain. Thus, studying of pre-existing immunity to the 2009 pandemic H1N1 virus (2009 H1N1) will advance our understanding of the pathogenesis and epidemiology of this emerging pathogen. In...
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| Veröffentlicht in: | PloS one 2013-03, Vol.8 (3), p.e58810-e58810 |
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| creator | Liu, Xiaoqing Liu, Yuan Zhang, Yanjun Chen, Zhihui Tang, Ziwei Xu, Qingqiang Wang, Yue Zhao, Ping Qi, Zhongtian |
| description | Pre-existing immunity is an important factor countering the pandemic potential of an emerging influenza virus strain. Thus, studying of pre-existing immunity to the 2009 pandemic H1N1 virus (2009 H1N1) will advance our understanding of the pathogenesis and epidemiology of this emerging pathogen. In the present study, sera were collected from 486 individuals in a hospital in Shanghai, China, before the 2009 H1N1 influenza pandemic. The serum anti-hemagglutinins (HA) antibody, hemagglutination inhibition (HI) antibody and neutralizing antibody against the 2009 H1N1 were assayed. Among this population, 84.2%, 14.61% and 26.5% subjects possessed anti-HA antibody, HI antibody and neutralizing antibody, respectively. Although neutralizing antibody only existed in those sera with detectable anti-HA antibody, there was no obvious correlation between the titers of anti-HA and neutralizing antibody. However, the titers of anti-HA and neutralizing antibody against seasonal H1N1 virus were highly correlated. In the same population, there was no correlation between titers of neutralizing antibody against 2009 H1N1 and seasonal H1N1. DNA immunization performed on mice demonstrated that antibodies to the HA of 2009 pandemic and seasonal H1N1 influenza viruses were strain-specific and had no cross-neutralizing activity. In addition, the predicted conserved epitope in the HA of 2009 H1N1 and recently circulating seasonal H1N1 virus, GLFGAIAGFIE, was not an immunologically valid B-cell epitope. The data in this report are valuable for advancing our understanding of 2009 H1N1 influenza virus infection. |
| doi_str_mv | 10.1371/journal.pone.0058810 |
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Thus, studying of pre-existing immunity to the 2009 pandemic H1N1 virus (2009 H1N1) will advance our understanding of the pathogenesis and epidemiology of this emerging pathogen. In the present study, sera were collected from 486 individuals in a hospital in Shanghai, China, before the 2009 H1N1 influenza pandemic. The serum anti-hemagglutinins (HA) antibody, hemagglutination inhibition (HI) antibody and neutralizing antibody against the 2009 H1N1 were assayed. Among this population, 84.2%, 14.61% and 26.5% subjects possessed anti-HA antibody, HI antibody and neutralizing antibody, respectively. Although neutralizing antibody only existed in those sera with detectable anti-HA antibody, there was no obvious correlation between the titers of anti-HA and neutralizing antibody. However, the titers of anti-HA and neutralizing antibody against seasonal H1N1 virus were highly correlated. In the same population, there was no correlation between titers of neutralizing antibody against 2009 H1N1 and seasonal H1N1. DNA immunization performed on mice demonstrated that antibodies to the HA of 2009 pandemic and seasonal H1N1 influenza viruses were strain-specific and had no cross-neutralizing activity. In addition, the predicted conserved epitope in the HA of 2009 H1N1 and recently circulating seasonal H1N1 virus, GLFGAIAGFIE, was not an immunologically valid B-cell epitope. The data in this report are valuable for advancing our understanding of 2009 H1N1 influenza virus infection.</description><identifier>ISSN: 1932-6203</identifier><identifier>EISSN: 1932-6203</identifier><identifier>DOI: 10.1371/journal.pone.0058810</identifier><identifier>PMID: 23527030</identifier><language>eng</language><publisher>United States: Public Library of Science</publisher><subject>Adolescent ; Adult ; Aged ; Aged, 80 and over ; Amino Acid Sequence ; Animals ; Antibodies ; Antibodies, Neutralizing ; Antibodies, Viral - blood ; Antibodies, Viral - immunology ; Antibody Specificity ; Antigenic determinants ; Binding sites ; Biology ; Cell Line ; China ; Correlation ; Cross Reactions - immunology ; Deoxyribonucleic acid ; Disease control ; Disease prevention ; DNA ; Epidemiology ; Epitopes ; Epitopes, B-Lymphocyte - chemistry ; Epitopes, B-Lymphocyte - immunology ; Evolution & development ; Female ; Health aspects ; Hemagglutination inhibition ; Hemagglutinin Glycoproteins, Influenza Virus - immunology ; Hemagglutinins ; Humans ; Immunity ; Immunization ; Immunoglobulin G - blood ; Immunoglobulin G - immunology ; Immunoglobulins ; Infections ; Influenza ; Influenza A Virus, H1N1 Subtype - immunology ; Influenza viruses ; Influenza, Human - epidemiology ; Influenza, Human - immunology ; Influenza, Human - prevention & control ; Laboratories ; Lymphocytes B ; Male ; Medicine ; Mice ; Middle Aged ; Neutralization Tests ; Neutralizing ; Orthomyxoviridae ; Pandemics ; Pathogenesis ; Plasmids ; Population ; Swine flu ; Swine influenza ; Vaccines ; Viruses ; Young Adult</subject><ispartof>PloS one, 2013-03, Vol.8 (3), p.e58810-e58810</ispartof><rights>COPYRIGHT 2013 Public Library of Science</rights><rights>2013 Liu et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License: https://creativecommons.org/licenses/by/4.0/ (the “License”), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><rights>2013 Liu et al 2013 Liu et al</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c692t-11721a7e2565dae1a9499a71047e065bf4e3b443a7f4bf37a25336e98ffc83733</citedby><cites>FETCH-LOGICAL-c692t-11721a7e2565dae1a9499a71047e065bf4e3b443a7f4bf37a25336e98ffc83733</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC3602581/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC3602581/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,727,780,784,864,885,2102,2928,23866,27924,27925,53791,53793,79600,79601</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/23527030$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><contributor>Khudyakov, Yury E.</contributor><creatorcontrib>Liu, Xiaoqing</creatorcontrib><creatorcontrib>Liu, Yuan</creatorcontrib><creatorcontrib>Zhang, Yanjun</creatorcontrib><creatorcontrib>Chen, Zhihui</creatorcontrib><creatorcontrib>Tang, Ziwei</creatorcontrib><creatorcontrib>Xu, Qingqiang</creatorcontrib><creatorcontrib>Wang, Yue</creatorcontrib><creatorcontrib>Zhao, Ping</creatorcontrib><creatorcontrib>Qi, Zhongtian</creatorcontrib><title>Pre-existing immunity with high neutralizing activity to 2009 pandemic H1N1 influenza virus in Shanghai population</title><title>PloS one</title><addtitle>PLoS One</addtitle><description>Pre-existing immunity is an important factor countering the pandemic potential of an emerging influenza virus strain. Thus, studying of pre-existing immunity to the 2009 pandemic H1N1 virus (2009 H1N1) will advance our understanding of the pathogenesis and epidemiology of this emerging pathogen. In the present study, sera were collected from 486 individuals in a hospital in Shanghai, China, before the 2009 H1N1 influenza pandemic. The serum anti-hemagglutinins (HA) antibody, hemagglutination inhibition (HI) antibody and neutralizing antibody against the 2009 H1N1 were assayed. Among this population, 84.2%, 14.61% and 26.5% subjects possessed anti-HA antibody, HI antibody and neutralizing antibody, respectively. Although neutralizing antibody only existed in those sera with detectable anti-HA antibody, there was no obvious correlation between the titers of anti-HA and neutralizing antibody. However, the titers of anti-HA and neutralizing antibody against seasonal H1N1 virus were highly correlated. In the same population, there was no correlation between titers of neutralizing antibody against 2009 H1N1 and seasonal H1N1. DNA immunization performed on mice demonstrated that antibodies to the HA of 2009 pandemic and seasonal H1N1 influenza viruses were strain-specific and had no cross-neutralizing activity. In addition, the predicted conserved epitope in the HA of 2009 H1N1 and recently circulating seasonal H1N1 virus, GLFGAIAGFIE, was not an immunologically valid B-cell epitope. The data in this report are valuable for advancing our understanding of 2009 H1N1 influenza virus infection.</description><subject>Adolescent</subject><subject>Adult</subject><subject>Aged</subject><subject>Aged, 80 and over</subject><subject>Amino Acid Sequence</subject><subject>Animals</subject><subject>Antibodies</subject><subject>Antibodies, Neutralizing</subject><subject>Antibodies, Viral - blood</subject><subject>Antibodies, Viral - immunology</subject><subject>Antibody Specificity</subject><subject>Antigenic determinants</subject><subject>Binding sites</subject><subject>Biology</subject><subject>Cell Line</subject><subject>China</subject><subject>Correlation</subject><subject>Cross Reactions - immunology</subject><subject>Deoxyribonucleic acid</subject><subject>Disease control</subject><subject>Disease prevention</subject><subject>DNA</subject><subject>Epidemiology</subject><subject>Epitopes</subject><subject>Epitopes, B-Lymphocyte - chemistry</subject><subject>Epitopes, B-Lymphocyte - immunology</subject><subject>Evolution & development</subject><subject>Female</subject><subject>Health aspects</subject><subject>Hemagglutination inhibition</subject><subject>Hemagglutinin Glycoproteins, Influenza Virus - immunology</subject><subject>Hemagglutinins</subject><subject>Humans</subject><subject>Immunity</subject><subject>Immunization</subject><subject>Immunoglobulin G - blood</subject><subject>Immunoglobulin G - immunology</subject><subject>Immunoglobulins</subject><subject>Infections</subject><subject>Influenza</subject><subject>Influenza A Virus, H1N1 Subtype - immunology</subject><subject>Influenza viruses</subject><subject>Influenza, Human - epidemiology</subject><subject>Influenza, Human - immunology</subject><subject>Influenza, Human - prevention & control</subject><subject>Laboratories</subject><subject>Lymphocytes B</subject><subject>Male</subject><subject>Medicine</subject><subject>Mice</subject><subject>Middle Aged</subject><subject>Neutralization Tests</subject><subject>Neutralizing</subject><subject>Orthomyxoviridae</subject><subject>Pandemics</subject><subject>Pathogenesis</subject><subject>Plasmids</subject><subject>Population</subject><subject>Swine flu</subject><subject>Swine influenza</subject><subject>Vaccines</subject><subject>Viruses</subject><subject>Young 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E.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Pre-existing immunity with high neutralizing activity to 2009 pandemic H1N1 influenza virus in Shanghai population</atitle><jtitle>PloS one</jtitle><addtitle>PLoS One</addtitle><date>2013-03-19</date><risdate>2013</risdate><volume>8</volume><issue>3</issue><spage>e58810</spage><epage>e58810</epage><pages>e58810-e58810</pages><issn>1932-6203</issn><eissn>1932-6203</eissn><abstract>Pre-existing immunity is an important factor countering the pandemic potential of an emerging influenza virus strain. Thus, studying of pre-existing immunity to the 2009 pandemic H1N1 virus (2009 H1N1) will advance our understanding of the pathogenesis and epidemiology of this emerging pathogen. In the present study, sera were collected from 486 individuals in a hospital in Shanghai, China, before the 2009 H1N1 influenza pandemic. The serum anti-hemagglutinins (HA) antibody, hemagglutination inhibition (HI) antibody and neutralizing antibody against the 2009 H1N1 were assayed. Among this population, 84.2%, 14.61% and 26.5% subjects possessed anti-HA antibody, HI antibody and neutralizing antibody, respectively. Although neutralizing antibody only existed in those sera with detectable anti-HA antibody, there was no obvious correlation between the titers of anti-HA and neutralizing antibody. However, the titers of anti-HA and neutralizing antibody against seasonal H1N1 virus were highly correlated. In the same population, there was no correlation between titers of neutralizing antibody against 2009 H1N1 and seasonal H1N1. DNA immunization performed on mice demonstrated that antibodies to the HA of 2009 pandemic and seasonal H1N1 influenza viruses were strain-specific and had no cross-neutralizing activity. In addition, the predicted conserved epitope in the HA of 2009 H1N1 and recently circulating seasonal H1N1 virus, GLFGAIAGFIE, was not an immunologically valid B-cell epitope. The data in this report are valuable for advancing our understanding of 2009 H1N1 influenza virus infection.</abstract><cop>United States</cop><pub>Public Library of Science</pub><pmid>23527030</pmid><doi>10.1371/journal.pone.0058810</doi><tpages>e58810</tpages><oa>free_for_read</oa></addata></record> |
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| issn | 1932-6203 1932-6203 |
| language | eng |
| recordid | cdi_plos_journals_1330889433 |
| source | MEDLINE; DOAJ Directory of Open Access Journals; Public Library of Science (PLoS); EZB-FREE-00999 freely available EZB journals; PubMed Central; Free Full-Text Journals in Chemistry |
| subjects | Adolescent Adult Aged Aged, 80 and over Amino Acid Sequence Animals Antibodies Antibodies, Neutralizing Antibodies, Viral - blood Antibodies, Viral - immunology Antibody Specificity Antigenic determinants Binding sites Biology Cell Line China Correlation Cross Reactions - immunology Deoxyribonucleic acid Disease control Disease prevention DNA Epidemiology Epitopes Epitopes, B-Lymphocyte - chemistry Epitopes, B-Lymphocyte - immunology Evolution & development Female Health aspects Hemagglutination inhibition Hemagglutinin Glycoproteins, Influenza Virus - immunology Hemagglutinins Humans Immunity Immunization Immunoglobulin G - blood Immunoglobulin G - immunology Immunoglobulins Infections Influenza Influenza A Virus, H1N1 Subtype - immunology Influenza viruses Influenza, Human - epidemiology Influenza, Human - immunology Influenza, Human - prevention & control Laboratories Lymphocytes B Male Medicine Mice Middle Aged Neutralization Tests Neutralizing Orthomyxoviridae Pandemics Pathogenesis Plasmids Population Swine flu Swine influenza Vaccines Viruses Young Adult |
| title | Pre-existing immunity with high neutralizing activity to 2009 pandemic H1N1 influenza virus in Shanghai population |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-05T22%3A03%3A22IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-gale_plos_&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Pre-existing%20immunity%20with%20high%20neutralizing%20activity%20to%202009%20pandemic%20H1N1%20influenza%20virus%20in%20Shanghai%20population&rft.jtitle=PloS%20one&rft.au=Liu,%20Xiaoqing&rft.date=2013-03-19&rft.volume=8&rft.issue=3&rft.spage=e58810&rft.epage=e58810&rft.pages=e58810-e58810&rft.issn=1932-6203&rft.eissn=1932-6203&rft_id=info:doi/10.1371/journal.pone.0058810&rft_dat=%3Cgale_plos_%3EA478207619%3C/gale_plos_%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=1330889433&rft_id=info:pmid/23527030&rft_galeid=A478207619&rft_doaj_id=oai_doaj_org_article_b58157b73d924b5ebddd23f992510b2a&rfr_iscdi=true |