Gene expression networks underlying ovarian development in wild largemouth bass (Micropterus salmoides)
Oocyte maturation in fish involves numerous cell signaling cascades that are activated or inhibited during specific stages of oocyte development. The objectives of this study were to characterize molecular pathways and temporal gene expression patterns throughout a complete breeding cycle in wild fe...
Gespeichert in:
| Veröffentlicht in: | PloS one 2013-03, Vol.8 (3), p.e59093 |
|---|---|
| Hauptverfasser: | , , , , , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | |
|---|---|
| container_issue | 3 |
| container_start_page | e59093 |
| container_title | PloS one |
| container_volume | 8 |
| creator | Martyniuk, Christopher J Prucha, Melinda S Doperalski, Nicholas J Antczak, Philipp Kroll, Kevin J Falciani, Francesco Barber, David S Denslow, Nancy D |
| description | Oocyte maturation in fish involves numerous cell signaling cascades that are activated or inhibited during specific stages of oocyte development. The objectives of this study were to characterize molecular pathways and temporal gene expression patterns throughout a complete breeding cycle in wild female largemouth bass to improve understanding of the molecular sequence of events underlying oocyte maturation.
Transcriptomic analysis was performed on eight morphologically diverse stages of the ovary, including primary and secondary stages of oocyte growth, ovulation, and atresia. Ovary histology, plasma vitellogenin, 17β-estradiol, and testosterone were also measured to correlate with gene networks.
Global expression patterns revealed dramatic differences across ovarian development, with 552 and 2070 genes being differentially expressed during both ovulation and atresia respectively. Gene set enrichment analysis (GSEA) revealed that early primary stages of oocyte growth involved increases in expression of genes involved in pathways of B-cell and T-cell receptor-mediated signaling cascades and fibronectin regulation. These pathways as well as pathways that included adrenergic receptor signaling, sphingolipid metabolism and natural killer cell activation were down-regulated at ovulation. At atresia, down-regulated pathways included gap junction and actin cytoskeleton regulation, gonadotrope and mast cell activation, and vasopressin receptor signaling and up-regulated pathways included oxidative phosphorylation and reactive oxygen species metabolism. Expression targets for luteinizing hormone signaling were low during vitellogenesis but increased 150% at ovulation. Other networks found to play a significant role in oocyte maturation included those with genes regulated by members of the TGF-beta superfamily (activins, inhibins, bone morphogenic protein 7 and growth differentiation factor 9), neuregulin 1, retinoid X receptor, and nerve growth factor family.
This study offers novel insight into the gene networks underlying vitellogenesis, ovulation and atresia and generates new hypotheses about the cellular pathways regulating oocyte maturation. |
| doi_str_mv | 10.1371/journal.pone.0059093 |
| format | Article |
| fullrecord | <record><control><sourceid>gale_plos_</sourceid><recordid>TN_cdi_plos_journals_1330889403</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><galeid>A478193021</galeid><doaj_id>oai_doaj_org_article_a7f72f3651394754a8c0540116ac11d9</doaj_id><sourcerecordid>A478193021</sourcerecordid><originalsourceid>FETCH-LOGICAL-c692t-569cac268dba1f37ff3549b1db3f37825d872f34c3324514ce015f7277b5e1c43</originalsourceid><addsrcrecordid>eNqNkl1rFDEUhgdRbK3-A9GAIPZi12SSzMeNUIrWhUrBr9uQyZyZzZpJpsnMtv33Zt1p2QEFyUW-nvPm8OZNkpcELwnNyfuNG72VZtk7C0uMeYlL-ig5JiVNF1mK6eOD9VHyLIRNhGiRZU-To5TyNMclP07aC7CA4Lb3EIJ2FlkYbpz_FdBoa_DmTtsWua30WlpUwxaM6zuwA9IW3WhTIyN9C50bhzWqZAjo3RetvOsH8GNAQZrO6RrC6fPkSSNNgBfTfJL8-PTx-_nnxeXVxer87HKhsjIdFjwrlVRpVtSVJA3Nm4ZyVlakrmjcFSmvizxtKFOUpowTpgAT3uRpnlcciGL0JHm91-2NC2LyKAhCKS6KkmEaidWeqJ3ciN7rTvo74aQWfw6cb4X0g1YGhMyb3WsZJ7RkOWeyUJgzTEgmFSF1GbU-TK-NVQe1isZ4aWai8xur16J1W0EzzAjetftmEvDueoQw_KPliWpl7ErbxkUx1emgxBnLi_jNOCWRWv6FiqOGTquYkkbH81nB6awgMgPcDq0cQxCrb1__n736OWffHrBrkGZYB2fGIeYrzEG2B2NiQvDQPDhHsNiF_N4NsQu5mEIey14duv5QdJ9q-hvgk_ea</addsrcrecordid><sourcetype>Open Website</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>1330889403</pqid></control><display><type>article</type><title>Gene expression networks underlying ovarian development in wild largemouth bass (Micropterus salmoides)</title><source>Public Library of Science (PLoS) Journals Open Access</source><source>MEDLINE</source><source>DOAJ Directory of Open Access Journals</source><source>Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals</source><source>PubMed Central</source><source>Free Full-Text Journals in Chemistry</source><creator>Martyniuk, Christopher J ; Prucha, Melinda S ; Doperalski, Nicholas J ; Antczak, Philipp ; Kroll, Kevin J ; Falciani, Francesco ; Barber, David S ; Denslow, Nancy D</creator><contributor>Foulkes, Nicholas S.</contributor><creatorcontrib>Martyniuk, Christopher J ; Prucha, Melinda S ; Doperalski, Nicholas J ; Antczak, Philipp ; Kroll, Kevin J ; Falciani, Francesco ; Barber, David S ; Denslow, Nancy D ; Foulkes, Nicholas S.</creatorcontrib><description>Oocyte maturation in fish involves numerous cell signaling cascades that are activated or inhibited during specific stages of oocyte development. The objectives of this study were to characterize molecular pathways and temporal gene expression patterns throughout a complete breeding cycle in wild female largemouth bass to improve understanding of the molecular sequence of events underlying oocyte maturation.
Transcriptomic analysis was performed on eight morphologically diverse stages of the ovary, including primary and secondary stages of oocyte growth, ovulation, and atresia. Ovary histology, plasma vitellogenin, 17β-estradiol, and testosterone were also measured to correlate with gene networks.
Global expression patterns revealed dramatic differences across ovarian development, with 552 and 2070 genes being differentially expressed during both ovulation and atresia respectively. Gene set enrichment analysis (GSEA) revealed that early primary stages of oocyte growth involved increases in expression of genes involved in pathways of B-cell and T-cell receptor-mediated signaling cascades and fibronectin regulation. These pathways as well as pathways that included adrenergic receptor signaling, sphingolipid metabolism and natural killer cell activation were down-regulated at ovulation. At atresia, down-regulated pathways included gap junction and actin cytoskeleton regulation, gonadotrope and mast cell activation, and vasopressin receptor signaling and up-regulated pathways included oxidative phosphorylation and reactive oxygen species metabolism. Expression targets for luteinizing hormone signaling were low during vitellogenesis but increased 150% at ovulation. Other networks found to play a significant role in oocyte maturation included those with genes regulated by members of the TGF-beta superfamily (activins, inhibins, bone morphogenic protein 7 and growth differentiation factor 9), neuregulin 1, retinoid X receptor, and nerve growth factor family.
This study offers novel insight into the gene networks underlying vitellogenesis, ovulation and atresia and generates new hypotheses about the cellular pathways regulating oocyte maturation.</description><identifier>ISSN: 1932-6203</identifier><identifier>EISSN: 1932-6203</identifier><identifier>DOI: 10.1371/journal.pone.0059093</identifier><identifier>PMID: 23527095</identifier><language>eng</language><publisher>United States: Public Library of Science</publisher><subject>17β-Estradiol ; Actin ; Activation ; Adrenergic receptors ; Analysis ; Animal breeding ; Animals ; Bass ; Bass - genetics ; Bass - metabolism ; beta Catenin - metabolism ; Biology ; Breeding ; Cascades ; Catfish ; Cell activation ; Cloning ; Cluster Analysis ; Computational Biology ; Correlation analysis ; Cytoskeleton ; Developmental stages ; Eggs ; Estradiol ; Estrogens - metabolism ; Female ; Females ; Fibronectin ; Fish ; Gene expression ; Gene Expression Profiling ; Gene Expression Regulation, Developmental ; Gene Regulatory Networks ; Gene set enrichment analysis ; Genes ; Growth differentiation factor 9 ; Histology ; Ictalurus punctatus ; Insulin-like growth factors ; Integrin alpha5beta1 - metabolism ; Lipid metabolism ; Luteinizing hormone ; Lymphocytes B ; Lymphocytes T ; Male ; Maturation ; Metabolism ; Micropterus salmoides ; Morphology ; Natural killer cells ; Nerve growth factor ; Networks ; Neuregulin ; Neuregulin 1 ; Oncorhynchus mykiss ; Oocytes ; Oogenesis - genetics ; Oreochromis niloticus ; Ovary - anatomy & histology ; Ovary - cytology ; Ovary - metabolism ; Ovulation ; Oxidative phosphorylation ; Oxygen ; Pathways ; Phosphorylation ; Physiology ; Pituitary (anterior) ; Reactive oxygen species ; Reproduction - genetics ; Salvelinus fontinalis ; Sex hormones ; Signal Transduction ; Signaling ; T cell receptors ; T-cell receptor ; Testosterone ; Toxicology ; Transforming growth factor-b ; Trout ; Vasopressin ; Vitellogenins - metabolism ; Zebrafish</subject><ispartof>PloS one, 2013-03, Vol.8 (3), p.e59093</ispartof><rights>COPYRIGHT 2013 Public Library of Science</rights><rights>2013 Martyniuk et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License: https://creativecommons.org/licenses/by/4.0/ (the “License”), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><rights>2013 Martyniuk et al 2013 Martyniuk et al</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c692t-569cac268dba1f37ff3549b1db3f37825d872f34c3324514ce015f7277b5e1c43</citedby><cites>FETCH-LOGICAL-c692t-569cac268dba1f37ff3549b1db3f37825d872f34c3324514ce015f7277b5e1c43</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC3604104/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC3604104/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,723,776,780,860,881,2096,2915,23845,27901,27902,53766,53768,79343,79344</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/23527095$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><contributor>Foulkes, Nicholas S.</contributor><creatorcontrib>Martyniuk, Christopher J</creatorcontrib><creatorcontrib>Prucha, Melinda S</creatorcontrib><creatorcontrib>Doperalski, Nicholas J</creatorcontrib><creatorcontrib>Antczak, Philipp</creatorcontrib><creatorcontrib>Kroll, Kevin J</creatorcontrib><creatorcontrib>Falciani, Francesco</creatorcontrib><creatorcontrib>Barber, David S</creatorcontrib><creatorcontrib>Denslow, Nancy D</creatorcontrib><title>Gene expression networks underlying ovarian development in wild largemouth bass (Micropterus salmoides)</title><title>PloS one</title><addtitle>PLoS One</addtitle><description>Oocyte maturation in fish involves numerous cell signaling cascades that are activated or inhibited during specific stages of oocyte development. The objectives of this study were to characterize molecular pathways and temporal gene expression patterns throughout a complete breeding cycle in wild female largemouth bass to improve understanding of the molecular sequence of events underlying oocyte maturation.
Transcriptomic analysis was performed on eight morphologically diverse stages of the ovary, including primary and secondary stages of oocyte growth, ovulation, and atresia. Ovary histology, plasma vitellogenin, 17β-estradiol, and testosterone were also measured to correlate with gene networks.
Global expression patterns revealed dramatic differences across ovarian development, with 552 and 2070 genes being differentially expressed during both ovulation and atresia respectively. Gene set enrichment analysis (GSEA) revealed that early primary stages of oocyte growth involved increases in expression of genes involved in pathways of B-cell and T-cell receptor-mediated signaling cascades and fibronectin regulation. These pathways as well as pathways that included adrenergic receptor signaling, sphingolipid metabolism and natural killer cell activation were down-regulated at ovulation. At atresia, down-regulated pathways included gap junction and actin cytoskeleton regulation, gonadotrope and mast cell activation, and vasopressin receptor signaling and up-regulated pathways included oxidative phosphorylation and reactive oxygen species metabolism. Expression targets for luteinizing hormone signaling were low during vitellogenesis but increased 150% at ovulation. Other networks found to play a significant role in oocyte maturation included those with genes regulated by members of the TGF-beta superfamily (activins, inhibins, bone morphogenic protein 7 and growth differentiation factor 9), neuregulin 1, retinoid X receptor, and nerve growth factor family.
This study offers novel insight into the gene networks underlying vitellogenesis, ovulation and atresia and generates new hypotheses about the cellular pathways regulating oocyte maturation.</description><subject>17β-Estradiol</subject><subject>Actin</subject><subject>Activation</subject><subject>Adrenergic receptors</subject><subject>Analysis</subject><subject>Animal breeding</subject><subject>Animals</subject><subject>Bass</subject><subject>Bass - genetics</subject><subject>Bass - metabolism</subject><subject>beta Catenin - metabolism</subject><subject>Biology</subject><subject>Breeding</subject><subject>Cascades</subject><subject>Catfish</subject><subject>Cell activation</subject><subject>Cloning</subject><subject>Cluster Analysis</subject><subject>Computational Biology</subject><subject>Correlation analysis</subject><subject>Cytoskeleton</subject><subject>Developmental stages</subject><subject>Eggs</subject><subject>Estradiol</subject><subject>Estrogens - metabolism</subject><subject>Female</subject><subject>Females</subject><subject>Fibronectin</subject><subject>Fish</subject><subject>Gene expression</subject><subject>Gene Expression Profiling</subject><subject>Gene Expression Regulation, Developmental</subject><subject>Gene Regulatory Networks</subject><subject>Gene set enrichment analysis</subject><subject>Genes</subject><subject>Growth differentiation factor 9</subject><subject>Histology</subject><subject>Ictalurus punctatus</subject><subject>Insulin-like growth factors</subject><subject>Integrin alpha5beta1 - metabolism</subject><subject>Lipid metabolism</subject><subject>Luteinizing hormone</subject><subject>Lymphocytes B</subject><subject>Lymphocytes T</subject><subject>Male</subject><subject>Maturation</subject><subject>Metabolism</subject><subject>Micropterus salmoides</subject><subject>Morphology</subject><subject>Natural killer cells</subject><subject>Nerve growth factor</subject><subject>Networks</subject><subject>Neuregulin</subject><subject>Neuregulin 1</subject><subject>Oncorhynchus mykiss</subject><subject>Oocytes</subject><subject>Oogenesis - genetics</subject><subject>Oreochromis niloticus</subject><subject>Ovary - anatomy & histology</subject><subject>Ovary - cytology</subject><subject>Ovary - metabolism</subject><subject>Ovulation</subject><subject>Oxidative phosphorylation</subject><subject>Oxygen</subject><subject>Pathways</subject><subject>Phosphorylation</subject><subject>Physiology</subject><subject>Pituitary (anterior)</subject><subject>Reactive oxygen species</subject><subject>Reproduction - genetics</subject><subject>Salvelinus fontinalis</subject><subject>Sex hormones</subject><subject>Signal Transduction</subject><subject>Signaling</subject><subject>T cell receptors</subject><subject>T-cell receptor</subject><subject>Testosterone</subject><subject>Toxicology</subject><subject>Transforming growth factor-b</subject><subject>Trout</subject><subject>Vasopressin</subject><subject>Vitellogenins - metabolism</subject><subject>Zebrafish</subject><issn>1932-6203</issn><issn>1932-6203</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2013</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>BENPR</sourceid><sourceid>DOA</sourceid><recordid>eNqNkl1rFDEUhgdRbK3-A9GAIPZi12SSzMeNUIrWhUrBr9uQyZyZzZpJpsnMtv33Zt1p2QEFyUW-nvPm8OZNkpcELwnNyfuNG72VZtk7C0uMeYlL-ig5JiVNF1mK6eOD9VHyLIRNhGiRZU-To5TyNMclP07aC7CA4Lb3EIJ2FlkYbpz_FdBoa_DmTtsWua30WlpUwxaM6zuwA9IW3WhTIyN9C50bhzWqZAjo3RetvOsH8GNAQZrO6RrC6fPkSSNNgBfTfJL8-PTx-_nnxeXVxer87HKhsjIdFjwrlVRpVtSVJA3Nm4ZyVlakrmjcFSmvizxtKFOUpowTpgAT3uRpnlcciGL0JHm91-2NC2LyKAhCKS6KkmEaidWeqJ3ciN7rTvo74aQWfw6cb4X0g1YGhMyb3WsZJ7RkOWeyUJgzTEgmFSF1GbU-TK-NVQe1isZ4aWai8xur16J1W0EzzAjetftmEvDueoQw_KPliWpl7ErbxkUx1emgxBnLi_jNOCWRWv6FiqOGTquYkkbH81nB6awgMgPcDq0cQxCrb1__n736OWffHrBrkGZYB2fGIeYrzEG2B2NiQvDQPDhHsNiF_N4NsQu5mEIey14duv5QdJ9q-hvgk_ea</recordid><startdate>20130320</startdate><enddate>20130320</enddate><creator>Martyniuk, Christopher J</creator><creator>Prucha, Melinda S</creator><creator>Doperalski, Nicholas J</creator><creator>Antczak, Philipp</creator><creator>Kroll, Kevin J</creator><creator>Falciani, Francesco</creator><creator>Barber, David S</creator><creator>Denslow, Nancy D</creator><general>Public Library of Science</general><general>Public Library of Science (PLoS)</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>IOV</scope><scope>ISR</scope><scope>3V.</scope><scope>7QG</scope><scope>7QL</scope><scope>7QO</scope><scope>7RV</scope><scope>7SN</scope><scope>7SS</scope><scope>7T5</scope><scope>7TG</scope><scope>7TM</scope><scope>7U9</scope><scope>7X2</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>8AO</scope><scope>8C1</scope><scope>8FD</scope><scope>8FE</scope><scope>8FG</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABJCF</scope><scope>ABUWG</scope><scope>AEUYN</scope><scope>AFKRA</scope><scope>ARAPS</scope><scope>ATCPS</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BGLVJ</scope><scope>BHPHI</scope><scope>C1K</scope><scope>CCPQU</scope><scope>D1I</scope><scope>DWQXO</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>H94</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>KB.</scope><scope>KB0</scope><scope>KL.</scope><scope>L6V</scope><scope>LK8</scope><scope>M0K</scope><scope>M0S</scope><scope>M1P</scope><scope>M7N</scope><scope>M7P</scope><scope>M7S</scope><scope>NAPCQ</scope><scope>P5Z</scope><scope>P62</scope><scope>P64</scope><scope>PATMY</scope><scope>PDBOC</scope><scope>PIMPY</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>PTHSS</scope><scope>PYCSY</scope><scope>RC3</scope><scope>5PM</scope><scope>DOA</scope></search><sort><creationdate>20130320</creationdate><title>Gene expression networks underlying ovarian development in wild largemouth bass (Micropterus salmoides)</title><author>Martyniuk, Christopher J ; Prucha, Melinda S ; Doperalski, Nicholas J ; Antczak, Philipp ; Kroll, Kevin J ; Falciani, Francesco ; Barber, David S ; Denslow, Nancy D</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c692t-569cac268dba1f37ff3549b1db3f37825d872f34c3324514ce015f7277b5e1c43</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2013</creationdate><topic>17β-Estradiol</topic><topic>Actin</topic><topic>Activation</topic><topic>Adrenergic receptors</topic><topic>Analysis</topic><topic>Animal breeding</topic><topic>Animals</topic><topic>Bass</topic><topic>Bass - genetics</topic><topic>Bass - metabolism</topic><topic>beta Catenin - metabolism</topic><topic>Biology</topic><topic>Breeding</topic><topic>Cascades</topic><topic>Catfish</topic><topic>Cell activation</topic><topic>Cloning</topic><topic>Cluster Analysis</topic><topic>Computational Biology</topic><topic>Correlation analysis</topic><topic>Cytoskeleton</topic><topic>Developmental stages</topic><topic>Eggs</topic><topic>Estradiol</topic><topic>Estrogens - metabolism</topic><topic>Female</topic><topic>Females</topic><topic>Fibronectin</topic><topic>Fish</topic><topic>Gene expression</topic><topic>Gene Expression Profiling</topic><topic>Gene Expression Regulation, Developmental</topic><topic>Gene Regulatory Networks</topic><topic>Gene set enrichment analysis</topic><topic>Genes</topic><topic>Growth differentiation factor 9</topic><topic>Histology</topic><topic>Ictalurus punctatus</topic><topic>Insulin-like growth factors</topic><topic>Integrin alpha5beta1 - metabolism</topic><topic>Lipid metabolism</topic><topic>Luteinizing hormone</topic><topic>Lymphocytes B</topic><topic>Lymphocytes T</topic><topic>Male</topic><topic>Maturation</topic><topic>Metabolism</topic><topic>Micropterus salmoides</topic><topic>Morphology</topic><topic>Natural killer cells</topic><topic>Nerve growth factor</topic><topic>Networks</topic><topic>Neuregulin</topic><topic>Neuregulin 1</topic><topic>Oncorhynchus mykiss</topic><topic>Oocytes</topic><topic>Oogenesis - genetics</topic><topic>Oreochromis niloticus</topic><topic>Ovary - anatomy & histology</topic><topic>Ovary - cytology</topic><topic>Ovary - metabolism</topic><topic>Ovulation</topic><topic>Oxidative phosphorylation</topic><topic>Oxygen</topic><topic>Pathways</topic><topic>Phosphorylation</topic><topic>Physiology</topic><topic>Pituitary (anterior)</topic><topic>Reactive oxygen species</topic><topic>Reproduction - genetics</topic><topic>Salvelinus fontinalis</topic><topic>Sex hormones</topic><topic>Signal Transduction</topic><topic>Signaling</topic><topic>T cell receptors</topic><topic>T-cell receptor</topic><topic>Testosterone</topic><topic>Toxicology</topic><topic>Transforming growth factor-b</topic><topic>Trout</topic><topic>Vasopressin</topic><topic>Vitellogenins - metabolism</topic><topic>Zebrafish</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Martyniuk, Christopher J</creatorcontrib><creatorcontrib>Prucha, Melinda S</creatorcontrib><creatorcontrib>Doperalski, Nicholas J</creatorcontrib><creatorcontrib>Antczak, Philipp</creatorcontrib><creatorcontrib>Kroll, Kevin J</creatorcontrib><creatorcontrib>Falciani, Francesco</creatorcontrib><creatorcontrib>Barber, David S</creatorcontrib><creatorcontrib>Denslow, Nancy D</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Gale In Context: Opposing Viewpoints</collection><collection>Gale In Context: Science</collection><collection>ProQuest Central (Corporate)</collection><collection>Animal Behavior Abstracts</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Biotechnology Research Abstracts</collection><collection>Proquest Nursing & Allied Health Source</collection><collection>Ecology Abstracts</collection><collection>Entomology Abstracts (Full archive)</collection><collection>Immunology Abstracts</collection><collection>Meteorological & Geoastrophysical Abstracts</collection><collection>Nucleic Acids Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>Agricultural Science Collection</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Medical Database (Alumni Edition)</collection><collection>ProQuest Pharma Collection</collection><collection>Public Health Database</collection><collection>Technology Research Database</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Technology Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>Materials Science & Engineering Collection</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest One Sustainability</collection><collection>ProQuest Central UK/Ireland</collection><collection>Advanced Technologies & Aerospace Collection</collection><collection>Agricultural & Environmental Science Collection</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Technology Collection</collection><collection>Natural Science Collection</collection><collection>Environmental Sciences and Pollution Management</collection><collection>ProQuest One Community College</collection><collection>ProQuest Materials Science Collection</collection><collection>ProQuest Central Korea</collection><collection>Engineering Research Database</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Materials Science Database</collection><collection>Nursing & Allied Health Database (Alumni Edition)</collection><collection>Meteorological & Geoastrophysical Abstracts - Academic</collection><collection>ProQuest Engineering Collection</collection><collection>ProQuest Biological Science Collection</collection><collection>Agricultural Science Database</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biological Science Database</collection><collection>Engineering Database</collection><collection>Nursing & Allied Health Premium</collection><collection>Advanced Technologies & Aerospace Database</collection><collection>ProQuest Advanced Technologies & Aerospace Collection</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Environmental Science Database</collection><collection>Materials Science Collection</collection><collection>Publicly Available Content Database</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><collection>Engineering Collection</collection><collection>Environmental Science Collection</collection><collection>Genetics Abstracts</collection><collection>PubMed Central (Full Participant titles)</collection><collection>DOAJ Directory of Open Access Journals</collection><jtitle>PloS one</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Martyniuk, Christopher J</au><au>Prucha, Melinda S</au><au>Doperalski, Nicholas J</au><au>Antczak, Philipp</au><au>Kroll, Kevin J</au><au>Falciani, Francesco</au><au>Barber, David S</au><au>Denslow, Nancy D</au><au>Foulkes, Nicholas S.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Gene expression networks underlying ovarian development in wild largemouth bass (Micropterus salmoides)</atitle><jtitle>PloS one</jtitle><addtitle>PLoS One</addtitle><date>2013-03-20</date><risdate>2013</risdate><volume>8</volume><issue>3</issue><spage>e59093</spage><pages>e59093-</pages><issn>1932-6203</issn><eissn>1932-6203</eissn><abstract>Oocyte maturation in fish involves numerous cell signaling cascades that are activated or inhibited during specific stages of oocyte development. The objectives of this study were to characterize molecular pathways and temporal gene expression patterns throughout a complete breeding cycle in wild female largemouth bass to improve understanding of the molecular sequence of events underlying oocyte maturation.
Transcriptomic analysis was performed on eight morphologically diverse stages of the ovary, including primary and secondary stages of oocyte growth, ovulation, and atresia. Ovary histology, plasma vitellogenin, 17β-estradiol, and testosterone were also measured to correlate with gene networks.
Global expression patterns revealed dramatic differences across ovarian development, with 552 and 2070 genes being differentially expressed during both ovulation and atresia respectively. Gene set enrichment analysis (GSEA) revealed that early primary stages of oocyte growth involved increases in expression of genes involved in pathways of B-cell and T-cell receptor-mediated signaling cascades and fibronectin regulation. These pathways as well as pathways that included adrenergic receptor signaling, sphingolipid metabolism and natural killer cell activation were down-regulated at ovulation. At atresia, down-regulated pathways included gap junction and actin cytoskeleton regulation, gonadotrope and mast cell activation, and vasopressin receptor signaling and up-regulated pathways included oxidative phosphorylation and reactive oxygen species metabolism. Expression targets for luteinizing hormone signaling were low during vitellogenesis but increased 150% at ovulation. Other networks found to play a significant role in oocyte maturation included those with genes regulated by members of the TGF-beta superfamily (activins, inhibins, bone morphogenic protein 7 and growth differentiation factor 9), neuregulin 1, retinoid X receptor, and nerve growth factor family.
This study offers novel insight into the gene networks underlying vitellogenesis, ovulation and atresia and generates new hypotheses about the cellular pathways regulating oocyte maturation.</abstract><cop>United States</cop><pub>Public Library of Science</pub><pmid>23527095</pmid><doi>10.1371/journal.pone.0059093</doi><tpages>e59093</tpages><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 1932-6203 |
| ispartof | PloS one, 2013-03, Vol.8 (3), p.e59093 |
| issn | 1932-6203 1932-6203 |
| language | eng |
| recordid | cdi_plos_journals_1330889403 |
| source | Public Library of Science (PLoS) Journals Open Access; MEDLINE; DOAJ Directory of Open Access Journals; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; PubMed Central; Free Full-Text Journals in Chemistry |
| subjects | 17β-Estradiol Actin Activation Adrenergic receptors Analysis Animal breeding Animals Bass Bass - genetics Bass - metabolism beta Catenin - metabolism Biology Breeding Cascades Catfish Cell activation Cloning Cluster Analysis Computational Biology Correlation analysis Cytoskeleton Developmental stages Eggs Estradiol Estrogens - metabolism Female Females Fibronectin Fish Gene expression Gene Expression Profiling Gene Expression Regulation, Developmental Gene Regulatory Networks Gene set enrichment analysis Genes Growth differentiation factor 9 Histology Ictalurus punctatus Insulin-like growth factors Integrin alpha5beta1 - metabolism Lipid metabolism Luteinizing hormone Lymphocytes B Lymphocytes T Male Maturation Metabolism Micropterus salmoides Morphology Natural killer cells Nerve growth factor Networks Neuregulin Neuregulin 1 Oncorhynchus mykiss Oocytes Oogenesis - genetics Oreochromis niloticus Ovary - anatomy & histology Ovary - cytology Ovary - metabolism Ovulation Oxidative phosphorylation Oxygen Pathways Phosphorylation Physiology Pituitary (anterior) Reactive oxygen species Reproduction - genetics Salvelinus fontinalis Sex hormones Signal Transduction Signaling T cell receptors T-cell receptor Testosterone Toxicology Transforming growth factor-b Trout Vasopressin Vitellogenins - metabolism Zebrafish |
| title | Gene expression networks underlying ovarian development in wild largemouth bass (Micropterus salmoides) |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-30T16%3A27%3A52IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-gale_plos_&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Gene%20expression%20networks%20underlying%20ovarian%20development%20in%20wild%20largemouth%20bass%20(Micropterus%20salmoides)&rft.jtitle=PloS%20one&rft.au=Martyniuk,%20Christopher%20J&rft.date=2013-03-20&rft.volume=8&rft.issue=3&rft.spage=e59093&rft.pages=e59093-&rft.issn=1932-6203&rft.eissn=1932-6203&rft_id=info:doi/10.1371/journal.pone.0059093&rft_dat=%3Cgale_plos_%3EA478193021%3C/gale_plos_%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=1330889403&rft_id=info:pmid/23527095&rft_galeid=A478193021&rft_doaj_id=oai_doaj_org_article_a7f72f3651394754a8c0540116ac11d9&rfr_iscdi=true |