Amplicon DNA melting analysis for the simultaneous detection of Brucella spp and Mycobacterium tuberculosis complex. Potential use in rapid differential diagnosis between extrapulmonary tuberculosis and focal complications of brucellosis

Some sites of extrapulmonary tuberculosis and focal complications of brucellosis are very difficult to differentiate clinically, radiologically, and even histopathologically. Conventional microbiological methods for the diagnosis of extrapulmonary tuberculosis and complicated brucellosis not only la...

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Veröffentlicht in:	PloS one 2013-03, Vol.8 (3), p.e58353-e58353
Hauptverfasser:	Sanjuan-Jimenez, Rocio, Colmenero, Juan D, Bermúdez, Pilar, Alonso, Antonio, Morata, Pilar
Format:	Artikel
Sprache:	eng
Schlagworte:	Analysis Analytical methods Assaying Biochemistry Biology Brucella Brucella - genetics Brucella abortus Brucellosis Brucellosis - diagnosis Brucellosis - genetics Brucellosis - microbiology Complications Deoxyribonucleic acid Design Detection limits Diagnosis Diagnosis, Differential Differential diagnosis DNA DNA, Bacterial - chemistry DNA, Bacterial - isolation & purification DNA, Bacterial - metabolism Equivalence Genes Genes, Bacterial Genetic analysis Genetic testing Genomes Genomics Humans Immunology Laboratories Medical diagnosis Medicine Melting Microorganisms Molecular biology Multiplexing Mycobacterium tuberculosis Mycobacterium tuberculosis - genetics Outer membrane proteins Pathogens Polymerase chain reaction Polymerase Chain Reaction - methods Primers Product differentiation Prognosis Real time Sensitivity and Specificity Tuberculosis Tuberculosis - diagnosis Tuberculosis - genetics Tuberculosis - microbiology
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