CRABP2 promotes myoblast differentiation and is modulated by the transcription factors MyoD and Sp1 in C2C12 cells
Cellular retinoic acid binding protein 2 (CRABP2), a member of a family of specific carrier proteins for Vitamin A, belongs to a family of small cytosolic lipid binding proteins. Our previous study suggested that CRABP2 was involved in skeletal muscle development; however, the molecular function and...
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| description | Cellular retinoic acid binding protein 2 (CRABP2), a member of a family of specific carrier proteins for Vitamin A, belongs to a family of small cytosolic lipid binding proteins. Our previous study suggested that CRABP2 was involved in skeletal muscle development; however, the molecular function and regulatory mechanism of CRABP2 in myogenesis remained unclear. In this study, we found that the expression of the CRABP2 gene was upregulated during C2C12 differentiation. An over-expression assay revealed that CRABP2 promotes myogenic transformation by regulating the cell cycle during C2C12 differentiation. The region from -459 to -4 bp was identified as the core promoter and contains a TATA box, a GC box and binding sites for the transcription factors MyoD and Sp1. Over-expression, site-directed mutagenesis and EMSA assays indicated that the transcription factors MyoD and Sp1 regulate CRABP2 expression and promote myoblast differentiation in C2C12 cells. |
| doi_str_mv | 10.1371/journal.pone.0055479 |
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Our previous study suggested that CRABP2 was involved in skeletal muscle development; however, the molecular function and regulatory mechanism of CRABP2 in myogenesis remained unclear. In this study, we found that the expression of the CRABP2 gene was upregulated during C2C12 differentiation. An over-expression assay revealed that CRABP2 promotes myogenic transformation by regulating the cell cycle during C2C12 differentiation. The region from -459 to -4 bp was identified as the core promoter and contains a TATA box, a GC box and binding sites for the transcription factors MyoD and Sp1. Over-expression, site-directed mutagenesis and EMSA assays indicated that the transcription factors MyoD and Sp1 regulate CRABP2 expression and promote myoblast differentiation in C2C12 cells.</description><identifier>ISSN: 1932-6203</identifier><identifier>EISSN: 1932-6203</identifier><identifier>DOI: 10.1371/journal.pone.0055479</identifier><identifier>PMID: 23383201</identifier><language>eng</language><publisher>United States: Public Library of Science</publisher><subject>Animal sciences ; Animals ; Binding sites ; Biology ; Cell cycle ; Cell differentiation ; Cell Differentiation - genetics ; Cell Line ; CRABP2 gene ; Deoxyribonucleic acid ; Differentiation ; DNA ; DNA binding proteins ; Gene expression ; Gene Expression Regulation ; Genes ; Genetic research ; Genetic transformation ; Genetic Vectors - genetics ; Humans ; Kinases ; Laboratories ; Lentivirus - genetics ; Mice ; Morphogenesis ; Morphology ; Muscles ; Musculoskeletal system ; Myoblasts - cytology ; Myoblasts - metabolism ; MyoD protein ; MyoD Protein - metabolism ; Myogenesis ; Nutrition ; Overexpression ; Phosphorylation ; Promoter Regions, Genetic ; Promoters (Genetics) ; Protein binding ; Proteins ; Receptors, Retinoic Acid - genetics ; Receptors, Retinoic Acid - metabolism ; Regulatory mechanisms (biology) ; Reproducibility of Results ; Retinoic acid ; RNA polymerase ; Rodents ; Site-directed mutagenesis ; Skeletal muscle ; Sp1 protein ; Sp1 Transcription Factor - metabolism ; Tata box ; Transcription factors ; Transcriptional Activation ; Transformation ; Vitamin A ; Zoology</subject><ispartof>PloS one, 2013-01, Vol.8 (1), p.e55479</ispartof><rights>COPYRIGHT 2013 Public Library of Science</rights><rights>2013 Yuan et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License: https://creativecommons.org/licenses/by/4.0/ (the “License”), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><rights>2013 Yuan et al 2013 Yuan et al</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c6079-a9c7b4bb268b47479db874e2bcfa266fbd2d28114a8acc44b03b4c6921dc5d683</citedby><cites>FETCH-LOGICAL-c6079-a9c7b4bb268b47479db874e2bcfa266fbd2d28114a8acc44b03b4c6921dc5d683</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC3561243/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC3561243/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,727,780,784,864,885,2102,2928,23866,27924,27925,53791,53793</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/23383201$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><contributor>Tang, Yao Liang</contributor><creatorcontrib>Yuan, Jing</creatorcontrib><creatorcontrib>Tang, Zhonglin</creatorcontrib><creatorcontrib>Yang, Shulin</creatorcontrib><creatorcontrib>Li, Kui</creatorcontrib><title>CRABP2 promotes myoblast differentiation and is modulated by the transcription factors MyoD and Sp1 in C2C12 cells</title><title>PloS one</title><addtitle>PLoS One</addtitle><description>Cellular retinoic acid binding protein 2 (CRABP2), a member of a family of specific carrier proteins for Vitamin A, belongs to a family of small cytosolic lipid binding proteins. Our previous study suggested that CRABP2 was involved in skeletal muscle development; however, the molecular function and regulatory mechanism of CRABP2 in myogenesis remained unclear. In this study, we found that the expression of the CRABP2 gene was upregulated during C2C12 differentiation. An over-expression assay revealed that CRABP2 promotes myogenic transformation by regulating the cell cycle during C2C12 differentiation. The region from -459 to -4 bp was identified as the core promoter and contains a TATA box, a GC box and binding sites for the transcription factors MyoD and Sp1. Over-expression, site-directed mutagenesis and EMSA assays indicated that the transcription factors MyoD and Sp1 regulate CRABP2 expression and promote myoblast differentiation in C2C12 cells.</description><subject>Animal sciences</subject><subject>Animals</subject><subject>Binding sites</subject><subject>Biology</subject><subject>Cell cycle</subject><subject>Cell differentiation</subject><subject>Cell Differentiation - genetics</subject><subject>Cell Line</subject><subject>CRABP2 gene</subject><subject>Deoxyribonucleic acid</subject><subject>Differentiation</subject><subject>DNA</subject><subject>DNA binding proteins</subject><subject>Gene expression</subject><subject>Gene Expression Regulation</subject><subject>Genes</subject><subject>Genetic research</subject><subject>Genetic transformation</subject><subject>Genetic Vectors - genetics</subject><subject>Humans</subject><subject>Kinases</subject><subject>Laboratories</subject><subject>Lentivirus - genetics</subject><subject>Mice</subject><subject>Morphogenesis</subject><subject>Morphology</subject><subject>Muscles</subject><subject>Musculoskeletal system</subject><subject>Myoblasts - 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Our previous study suggested that CRABP2 was involved in skeletal muscle development; however, the molecular function and regulatory mechanism of CRABP2 in myogenesis remained unclear. In this study, we found that the expression of the CRABP2 gene was upregulated during C2C12 differentiation. An over-expression assay revealed that CRABP2 promotes myogenic transformation by regulating the cell cycle during C2C12 differentiation. The region from -459 to -4 bp was identified as the core promoter and contains a TATA box, a GC box and binding sites for the transcription factors MyoD and Sp1. Over-expression, site-directed mutagenesis and EMSA assays indicated that the transcription factors MyoD and Sp1 regulate CRABP2 expression and promote myoblast differentiation in C2C12 cells.</abstract><cop>United States</cop><pub>Public Library of Science</pub><pmid>23383201</pmid><doi>10.1371/journal.pone.0055479</doi><tpages>e55479</tpages><oa>free_for_read</oa></addata></record> |
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| subjects | Animal sciences Animals Binding sites Biology Cell cycle Cell differentiation Cell Differentiation - genetics Cell Line CRABP2 gene Deoxyribonucleic acid Differentiation DNA DNA binding proteins Gene expression Gene Expression Regulation Genes Genetic research Genetic transformation Genetic Vectors - genetics Humans Kinases Laboratories Lentivirus - genetics Mice Morphogenesis Morphology Muscles Musculoskeletal system Myoblasts - cytology Myoblasts - metabolism MyoD protein MyoD Protein - metabolism Myogenesis Nutrition Overexpression Phosphorylation Promoter Regions, Genetic Promoters (Genetics) Protein binding Proteins Receptors, Retinoic Acid - genetics Receptors, Retinoic Acid - metabolism Regulatory mechanisms (biology) Reproducibility of Results Retinoic acid RNA polymerase Rodents Site-directed mutagenesis Skeletal muscle Sp1 protein Sp1 Transcription Factor - metabolism Tata box Transcription factors Transcriptional Activation Transformation Vitamin A Zoology |
| title | CRABP2 promotes myoblast differentiation and is modulated by the transcription factors MyoD and Sp1 in C2C12 cells |
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