Ischemic preconditioning increases endothelial progenitor cell number to attenuate partial nephrectomy-induced ischemia/reperfusion injury
The objective of this study was to investigate the role of endothelial progenitor cells (EPCs) in the modulation of ischemia-reperfusion injury (IRI) in a partial nephrectomy (PN) rat model using early-phase ischemic preconditioning (IPC). Ninety male Sprague-Dawley rats were randomly divided into t...
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| Veröffentlicht in: | PloS one 2013-01, Vol.8 (1), p.e55389-e55389 |
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| creator | Liu, Hao Wu, Ran Jia, Rui-Peng Zhong, Bing Zhu, Jia-Geng Yu, Peng Zhao, Yan Ge, Yu-Zheng Wu, Jian-Ping |
| description | The objective of this study was to investigate the role of endothelial progenitor cells (EPCs) in the modulation of ischemia-reperfusion injury (IRI) in a partial nephrectomy (PN) rat model using early-phase ischemic preconditioning (IPC).
Ninety male Sprague-Dawley rats were randomly divided into three groups following right-side nephrectomy: Sham-operated rats (surgery without vascular clamping); PN rats (renal blood vessels were clamped for 40 min and PN was performed); and IPC rats (pretreated with 15 min ischemia and 10 min reperfusion). At 1, 3, 6, 12, 24 h, and 3 days after reperfusion, the pool of circulating EPCs and kidneys were harvested. The extent of renal injury was assessed, along with EPC number, cell proliferation, angiogenesis, and vascular growth factor expression.
Pretreated rats exhibited significant improvements in renal function and morphology. EPC numbers in the kidneys were increased at 12 h following reperfusion in the IPC group as compared to the PN or Sham groups. Cell proliferation (including endothelial and tubular epithelial cells) and angiogenesis in peritubular capillaries were markedly increased in kidneys treated with IPC. In addition, vascular endothelial growth factor-A (VEGF-A) and stromal cell-derived factor-1α (SDF-1α) expression in the kidneys of pretreated rats was increased compared to rats subjected to PN.
OUR INVESTIGATION SUGGESTED THAT: (1) the early phase of IPC may attenuate renal IRI induced by PN; (2) EPCs play an important role in renal protection, involving promotion of cell proliferation and angiogenesis through release of several angiogenic factors. |
| doi_str_mv | 10.1371/journal.pone.0055389 |
| format | Article |
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Ninety male Sprague-Dawley rats were randomly divided into three groups following right-side nephrectomy: Sham-operated rats (surgery without vascular clamping); PN rats (renal blood vessels were clamped for 40 min and PN was performed); and IPC rats (pretreated with 15 min ischemia and 10 min reperfusion). At 1, 3, 6, 12, 24 h, and 3 days after reperfusion, the pool of circulating EPCs and kidneys were harvested. The extent of renal injury was assessed, along with EPC number, cell proliferation, angiogenesis, and vascular growth factor expression.
Pretreated rats exhibited significant improvements in renal function and morphology. EPC numbers in the kidneys were increased at 12 h following reperfusion in the IPC group as compared to the PN or Sham groups. Cell proliferation (including endothelial and tubular epithelial cells) and angiogenesis in peritubular capillaries were markedly increased in kidneys treated with IPC. In addition, vascular endothelial growth factor-A (VEGF-A) and stromal cell-derived factor-1α (SDF-1α) expression in the kidneys of pretreated rats was increased compared to rats subjected to PN.
OUR INVESTIGATION SUGGESTED THAT: (1) the early phase of IPC may attenuate renal IRI induced by PN; (2) EPCs play an important role in renal protection, involving promotion of cell proliferation and angiogenesis through release of several angiogenic factors.</description><identifier>ISSN: 1932-6203</identifier><identifier>EISSN: 1932-6203</identifier><identifier>DOI: 10.1371/journal.pone.0055389</identifier><identifier>PMID: 23383174</identifier><language>eng</language><publisher>United States: Public Library of Science</publisher><subject>Analysis of Variance ; Angiogenesis ; Animals ; Biology ; Blood vessels ; Blotting, Western ; Bone marrow ; Capillaries ; Cell number ; Cell proliferation ; Cells (biology) ; Chemokine CXCL12 - metabolism ; Clamping ; DNA Primers - genetics ; Endothelial Cells - physiology ; Endothelium ; Epithelial cells ; Flow Cytometry ; Fluorescent Antibody Technique ; Histological Techniques ; Hospitals ; Immunohistochemistry ; Injuries ; Ischemia ; Ischemic Preconditioning - methods ; Kidneys ; Male ; Medicine ; Neovascularization, Physiologic - physiology ; Nephrectomy ; Nephrectomy - adverse effects ; Polymerase Chain Reaction ; Preconditioning ; Progenitor cells ; Rats ; Rats, Sprague-Dawley ; Renal function ; Reperfusion ; Reperfusion Injury - etiology ; Reperfusion Injury - prevention & control ; Rodents ; Stem cells ; Stem Cells - physiology ; Studies ; Surgery ; Urology ; Vascular endothelial growth factor ; Vascular Endothelial Growth Factor A - metabolism ; Veins & arteries</subject><ispartof>PloS one, 2013-01, Vol.8 (1), p.e55389-e55389</ispartof><rights>COPYRIGHT 2013 Public Library of Science</rights><rights>2013 Liu et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License: https://creativecommons.org/licenses/by/4.0/ (the “License”), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><rights>2013 Liu et al 2013 Liu et al</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c758t-d98b27df50c5eab51b3719bc19679b21ac16ffb610f215445c1aac96ba3188ab3</citedby><cites>FETCH-LOGICAL-c758t-d98b27df50c5eab51b3719bc19679b21ac16ffb610f215445c1aac96ba3188ab3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC3561290/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC3561290/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,727,780,784,864,885,2100,2926,23865,27923,27924,53790,53792,79371,79372</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/23383174$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><contributor>Long, David</contributor><creatorcontrib>Liu, Hao</creatorcontrib><creatorcontrib>Wu, Ran</creatorcontrib><creatorcontrib>Jia, Rui-Peng</creatorcontrib><creatorcontrib>Zhong, Bing</creatorcontrib><creatorcontrib>Zhu, Jia-Geng</creatorcontrib><creatorcontrib>Yu, Peng</creatorcontrib><creatorcontrib>Zhao, Yan</creatorcontrib><creatorcontrib>Ge, Yu-Zheng</creatorcontrib><creatorcontrib>Wu, Jian-Ping</creatorcontrib><title>Ischemic preconditioning increases endothelial progenitor cell number to attenuate partial nephrectomy-induced ischemia/reperfusion injury</title><title>PloS one</title><addtitle>PLoS One</addtitle><description>The objective of this study was to investigate the role of endothelial progenitor cells (EPCs) in the modulation of ischemia-reperfusion injury (IRI) in a partial nephrectomy (PN) rat model using early-phase ischemic preconditioning (IPC).
Ninety male Sprague-Dawley rats were randomly divided into three groups following right-side nephrectomy: Sham-operated rats (surgery without vascular clamping); PN rats (renal blood vessels were clamped for 40 min and PN was performed); and IPC rats (pretreated with 15 min ischemia and 10 min reperfusion). At 1, 3, 6, 12, 24 h, and 3 days after reperfusion, the pool of circulating EPCs and kidneys were harvested. The extent of renal injury was assessed, along with EPC number, cell proliferation, angiogenesis, and vascular growth factor expression.
Pretreated rats exhibited significant improvements in renal function and morphology. EPC numbers in the kidneys were increased at 12 h following reperfusion in the IPC group as compared to the PN or Sham groups. Cell proliferation (including endothelial and tubular epithelial cells) and angiogenesis in peritubular capillaries were markedly increased in kidneys treated with IPC. In addition, vascular endothelial growth factor-A (VEGF-A) and stromal cell-derived factor-1α (SDF-1α) expression in the kidneys of pretreated rats was increased compared to rats subjected to PN.
OUR INVESTIGATION SUGGESTED THAT: (1) the early phase of IPC may attenuate renal IRI induced by PN; (2) EPCs play an important role in renal protection, involving promotion of cell proliferation and angiogenesis through release of several angiogenic factors.</description><subject>Analysis of Variance</subject><subject>Angiogenesis</subject><subject>Animals</subject><subject>Biology</subject><subject>Blood vessels</subject><subject>Blotting, Western</subject><subject>Bone marrow</subject><subject>Capillaries</subject><subject>Cell number</subject><subject>Cell proliferation</subject><subject>Cells (biology)</subject><subject>Chemokine CXCL12 - metabolism</subject><subject>Clamping</subject><subject>DNA Primers - genetics</subject><subject>Endothelial Cells - physiology</subject><subject>Endothelium</subject><subject>Epithelial cells</subject><subject>Flow Cytometry</subject><subject>Fluorescent Antibody Technique</subject><subject>Histological Techniques</subject><subject>Hospitals</subject><subject>Immunohistochemistry</subject><subject>Injuries</subject><subject>Ischemia</subject><subject>Ischemic Preconditioning - methods</subject><subject>Kidneys</subject><subject>Male</subject><subject>Medicine</subject><subject>Neovascularization, Physiologic - physiology</subject><subject>Nephrectomy</subject><subject>Nephrectomy - adverse effects</subject><subject>Polymerase Chain Reaction</subject><subject>Preconditioning</subject><subject>Progenitor cells</subject><subject>Rats</subject><subject>Rats, Sprague-Dawley</subject><subject>Renal function</subject><subject>Reperfusion</subject><subject>Reperfusion Injury - etiology</subject><subject>Reperfusion Injury - prevention & control</subject><subject>Rodents</subject><subject>Stem cells</subject><subject>Stem Cells - physiology</subject><subject>Studies</subject><subject>Surgery</subject><subject>Urology</subject><subject>Vascular endothelial growth factor</subject><subject>Vascular Endothelial Growth Factor A - metabolism</subject><subject>Veins & arteries</subject><issn>1932-6203</issn><issn>1932-6203</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2013</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><sourceid>DOA</sourceid><recordid>eNqNk92K1TAUhYsozjj6BqIFQfTinGmSJk1vhGHw58DAgH-3IU132xzapCapeF7Bpzb1dIapzIX0oiX99lrdq3snyXOUbREp0PneTs7IfjtaA9sso5Tw8kFyikqCNwxn5OGd55Pkiff7CBHO2OPkBBPCCSry0-T3zqsOBq3S0YGyptZBW6NNm2qjHEgPPgVT29BBr2UfKduC0cG6VEHfp2YaKnBpsKkMAcwkA6SjdGFmDYxdFA12OGy0qScFdaqPdvLcwQiumXx0i1b7yR2eJo8a2Xt4ttzPkm8f3n-9_LS5uv64u7y42qiC8rCpS17hom5opijIiqIqplFWCpWsKCuMpEKsaSqGsgYjmudUISlVySpJEOeyImfJy6Pu2Fsvlhi9QATzDGesoJHYHYnayr0YnR6kOwgrtfh7YF0r5hZVDwIrhBVHUNQY5YSSimVlAVSxHCmOaxa13i1uUzVArcAEJ_uV6PqN0Z1o7U9BKEO4zKLAm0XA2R8T-CCGGGLMXhqwU_xuzHOGMSV5RF_9g97f3UK1MjagTWOjr5pFxUVeFCVmvMCR2t5DxauepyXOXKPj-arg7aogMgF-hVZO3ovdl8__z15_X7Ov77AdyD503vbTPKd-DeZHUDnrvYPmNmSUiXllbtIQ88qIZWVi2Yu7P-i26GZHyB8HuxTg</recordid><startdate>20130131</startdate><enddate>20130131</enddate><creator>Liu, Hao</creator><creator>Wu, Ran</creator><creator>Jia, Rui-Peng</creator><creator>Zhong, Bing</creator><creator>Zhu, Jia-Geng</creator><creator>Yu, Peng</creator><creator>Zhao, Yan</creator><creator>Ge, Yu-Zheng</creator><creator>Wu, Jian-Ping</creator><general>Public Library of Science</general><general>Public Library of Science (PLoS)</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>IOV</scope><scope>ISR</scope><scope>3V.</scope><scope>7QG</scope><scope>7QL</scope><scope>7QO</scope><scope>7RV</scope><scope>7SN</scope><scope>7SS</scope><scope>7T5</scope><scope>7TG</scope><scope>7TM</scope><scope>7U9</scope><scope>7X2</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>8AO</scope><scope>8C1</scope><scope>8FD</scope><scope>8FE</scope><scope>8FG</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABJCF</scope><scope>ABUWG</scope><scope>AEUYN</scope><scope>AFKRA</scope><scope>ARAPS</scope><scope>ATCPS</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BGLVJ</scope><scope>BHPHI</scope><scope>C1K</scope><scope>CCPQU</scope><scope>D1I</scope><scope>DWQXO</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>H94</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>KB.</scope><scope>KB0</scope><scope>KL.</scope><scope>L6V</scope><scope>LK8</scope><scope>M0K</scope><scope>M0S</scope><scope>M1P</scope><scope>M7N</scope><scope>M7P</scope><scope>M7S</scope><scope>NAPCQ</scope><scope>P5Z</scope><scope>P62</scope><scope>P64</scope><scope>PATMY</scope><scope>PDBOC</scope><scope>PIMPY</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>PTHSS</scope><scope>PYCSY</scope><scope>RC3</scope><scope>7X8</scope><scope>5PM</scope><scope>DOA</scope></search><sort><creationdate>20130131</creationdate><title>Ischemic preconditioning increases endothelial progenitor cell number to attenuate partial nephrectomy-induced ischemia/reperfusion injury</title><author>Liu, Hao ; Wu, Ran ; Jia, Rui-Peng ; Zhong, Bing ; Zhu, Jia-Geng ; Yu, Peng ; Zhao, Yan ; Ge, Yu-Zheng ; Wu, Jian-Ping</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c758t-d98b27df50c5eab51b3719bc19679b21ac16ffb610f215445c1aac96ba3188ab3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2013</creationdate><topic>Analysis of Variance</topic><topic>Angiogenesis</topic><topic>Animals</topic><topic>Biology</topic><topic>Blood vessels</topic><topic>Blotting, Western</topic><topic>Bone marrow</topic><topic>Capillaries</topic><topic>Cell number</topic><topic>Cell proliferation</topic><topic>Cells (biology)</topic><topic>Chemokine CXCL12 - metabolism</topic><topic>Clamping</topic><topic>DNA Primers - genetics</topic><topic>Endothelial Cells - physiology</topic><topic>Endothelium</topic><topic>Epithelial cells</topic><topic>Flow Cytometry</topic><topic>Fluorescent Antibody Technique</topic><topic>Histological Techniques</topic><topic>Hospitals</topic><topic>Immunohistochemistry</topic><topic>Injuries</topic><topic>Ischemia</topic><topic>Ischemic Preconditioning - methods</topic><topic>Kidneys</topic><topic>Male</topic><topic>Medicine</topic><topic>Neovascularization, Physiologic - physiology</topic><topic>Nephrectomy</topic><topic>Nephrectomy - adverse effects</topic><topic>Polymerase Chain Reaction</topic><topic>Preconditioning</topic><topic>Progenitor cells</topic><topic>Rats</topic><topic>Rats, Sprague-Dawley</topic><topic>Renal function</topic><topic>Reperfusion</topic><topic>Reperfusion Injury - etiology</topic><topic>Reperfusion Injury - prevention & control</topic><topic>Rodents</topic><topic>Stem cells</topic><topic>Stem Cells - 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Academic</collection><collection>PubMed Central (Full Participant titles)</collection><collection>DOAJ Directory of Open Access Journals</collection><jtitle>PloS one</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Liu, Hao</au><au>Wu, Ran</au><au>Jia, Rui-Peng</au><au>Zhong, Bing</au><au>Zhu, Jia-Geng</au><au>Yu, Peng</au><au>Zhao, Yan</au><au>Ge, Yu-Zheng</au><au>Wu, Jian-Ping</au><au>Long, David</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Ischemic preconditioning increases endothelial progenitor cell number to attenuate partial nephrectomy-induced ischemia/reperfusion injury</atitle><jtitle>PloS one</jtitle><addtitle>PLoS One</addtitle><date>2013-01-31</date><risdate>2013</risdate><volume>8</volume><issue>1</issue><spage>e55389</spage><epage>e55389</epage><pages>e55389-e55389</pages><issn>1932-6203</issn><eissn>1932-6203</eissn><abstract>The objective of this study was to investigate the role of endothelial progenitor cells (EPCs) in the modulation of ischemia-reperfusion injury (IRI) in a partial nephrectomy (PN) rat model using early-phase ischemic preconditioning (IPC).
Ninety male Sprague-Dawley rats were randomly divided into three groups following right-side nephrectomy: Sham-operated rats (surgery without vascular clamping); PN rats (renal blood vessels were clamped for 40 min and PN was performed); and IPC rats (pretreated with 15 min ischemia and 10 min reperfusion). At 1, 3, 6, 12, 24 h, and 3 days after reperfusion, the pool of circulating EPCs and kidneys were harvested. The extent of renal injury was assessed, along with EPC number, cell proliferation, angiogenesis, and vascular growth factor expression.
Pretreated rats exhibited significant improvements in renal function and morphology. EPC numbers in the kidneys were increased at 12 h following reperfusion in the IPC group as compared to the PN or Sham groups. Cell proliferation (including endothelial and tubular epithelial cells) and angiogenesis in peritubular capillaries were markedly increased in kidneys treated with IPC. In addition, vascular endothelial growth factor-A (VEGF-A) and stromal cell-derived factor-1α (SDF-1α) expression in the kidneys of pretreated rats was increased compared to rats subjected to PN.
OUR INVESTIGATION SUGGESTED THAT: (1) the early phase of IPC may attenuate renal IRI induced by PN; (2) EPCs play an important role in renal protection, involving promotion of cell proliferation and angiogenesis through release of several angiogenic factors.</abstract><cop>United States</cop><pub>Public Library of Science</pub><pmid>23383174</pmid><doi>10.1371/journal.pone.0055389</doi><tpages>e55389</tpages><oa>free_for_read</oa></addata></record> |
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| identifier | ISSN: 1932-6203 |
| ispartof | PloS one, 2013-01, Vol.8 (1), p.e55389-e55389 |
| issn | 1932-6203 1932-6203 |
| language | eng |
| recordid | cdi_plos_journals_1328020675 |
| source | MEDLINE; DOAJ Directory of Open Access Journals; Public Library of Science (PLoS) Journals Open Access; EZB-FREE-00999 freely available EZB journals; PubMed Central; Free Full-Text Journals in Chemistry |
| subjects | Analysis of Variance Angiogenesis Animals Biology Blood vessels Blotting, Western Bone marrow Capillaries Cell number Cell proliferation Cells (biology) Chemokine CXCL12 - metabolism Clamping DNA Primers - genetics Endothelial Cells - physiology Endothelium Epithelial cells Flow Cytometry Fluorescent Antibody Technique Histological Techniques Hospitals Immunohistochemistry Injuries Ischemia Ischemic Preconditioning - methods Kidneys Male Medicine Neovascularization, Physiologic - physiology Nephrectomy Nephrectomy - adverse effects Polymerase Chain Reaction Preconditioning Progenitor cells Rats Rats, Sprague-Dawley Renal function Reperfusion Reperfusion Injury - etiology Reperfusion Injury - prevention & control Rodents Stem cells Stem Cells - physiology Studies Surgery Urology Vascular endothelial growth factor Vascular Endothelial Growth Factor A - metabolism Veins & arteries |
| title | Ischemic preconditioning increases endothelial progenitor cell number to attenuate partial nephrectomy-induced ischemia/reperfusion injury |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-10T23%3A13%3A01IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-gale_plos_&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Ischemic%20preconditioning%20increases%20endothelial%20progenitor%20cell%20number%20to%20attenuate%20partial%20nephrectomy-induced%20ischemia/reperfusion%20injury&rft.jtitle=PloS%20one&rft.au=Liu,%20Hao&rft.date=2013-01-31&rft.volume=8&rft.issue=1&rft.spage=e55389&rft.epage=e55389&rft.pages=e55389-e55389&rft.issn=1932-6203&rft.eissn=1932-6203&rft_id=info:doi/10.1371/journal.pone.0055389&rft_dat=%3Cgale_plos_%3EA477926872%3C/gale_plos_%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=1328020675&rft_id=info:pmid/23383174&rft_galeid=A477926872&rft_doaj_id=oai_doaj_org_article_2c12c81e7d214353b6097e5c641c82d6&rfr_iscdi=true |