Genetically matched human iPS cells reveal that propensity for cartilage and bone differentiation differs with clones, not cell type of origin
For regenerative therapy using induced pluripotent stem cell (iPSC) technology, cell type of origin to be reprogrammed should be chosen based on accessibility and reprogramming efficiency. Some studies report that iPSCs exhibited a preference for differentiation into their original cell lineages, wh...
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| creator | Nasu, Akira Ikeya, Makoto Yamamoto, Takuya Watanabe, Akira Jin, Yonghui Matsumoto, Yoshihisa Hayakawa, Kazuo Amano, Naoki Sato, Shingo Osafune, Kenji Aoyama, Tomoki Nakamura, Takashi Kato, Tomohisa Toguchida, Junya |
| description | For regenerative therapy using induced pluripotent stem cell (iPSC) technology, cell type of origin to be reprogrammed should be chosen based on accessibility and reprogramming efficiency. Some studies report that iPSCs exhibited a preference for differentiation into their original cell lineages, while others did not. Therefore, the type of cell which is most appropriate as a source for iPSCs needs to be clarified.
Genetically matched human iPSCs from different origins were generated using bone marrow stromal cells (BMSCs) and dermal fibroblasts (DFs) of the same donor, and global gene expression profile, DNA methylation status, and differentiation properties into the chondrogenic and osteogenic lineage of each clone were analyzed. Although genome-wide profiling of DNA methylation suggested tissue memory in iPSCs, genes expressed differentially in BMSCs and DFs were equally silenced in our bona fide iPSCs. After cell-autonomous and induced differentiation, each iPSC clone exhibited various differentiation properties, which did not correlate with cell-of-origin.
The reprogramming process may remove the difference between DFs and BMSCs at least for chondrogenic and osteogenic differentiation. Qualified and genetically matched human iPSC clone sets established in this study are valuable resources for further basic study of clonal differences. |
| doi_str_mv | 10.1371/journal.pone.0053771 |
| format | Article |
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Genetically matched human iPSCs from different origins were generated using bone marrow stromal cells (BMSCs) and dermal fibroblasts (DFs) of the same donor, and global gene expression profile, DNA methylation status, and differentiation properties into the chondrogenic and osteogenic lineage of each clone were analyzed. Although genome-wide profiling of DNA methylation suggested tissue memory in iPSCs, genes expressed differentially in BMSCs and DFs were equally silenced in our bona fide iPSCs. After cell-autonomous and induced differentiation, each iPSC clone exhibited various differentiation properties, which did not correlate with cell-of-origin.
The reprogramming process may remove the difference between DFs and BMSCs at least for chondrogenic and osteogenic differentiation. Qualified and genetically matched human iPSC clone sets established in this study are valuable resources for further basic study of clonal differences.</description><identifier>ISSN: 1932-6203</identifier><identifier>EISSN: 1932-6203</identifier><identifier>DOI: 10.1371/journal.pone.0053771</identifier><identifier>PMID: 23382851</identifier><language>eng</language><publisher>United States: Public Library of Science</publisher><subject>Analysis ; Animal behavior ; Biocompatibility ; Biology ; Biomedical materials ; Bone Development - genetics ; Bone marrow ; Bone marrow transplantation ; Cartilage ; Cartilage - cytology ; Cartilage - growth & development ; Cell Differentiation ; Cell growth ; Cell Lineage ; Clonal Evolution - genetics ; Cloning ; Deoxyribonucleic acid ; Differentiation ; DNA ; DNA fingerprinting ; DNA methylation ; DNA Methylation - genetics ; Efficiency ; Fibroblasts ; Fibroblasts - cytology ; Gene expression ; Gene Expression Regulation, Developmental ; Genes ; Genomes ; Genomics ; Humans ; Induced Pluripotent Stem Cells - cytology ; Medicine ; Mesenchymal stem cells ; Mesenchymal Stromal Cells - cytology ; Methylation ; Pluripotency ; Science ; Skin ; Stem cell transplantation ; Stem cells ; Stromal cells ; Surgery ; Tissue engineering</subject><ispartof>PloS one, 2013-01, Vol.8 (1), p.e53771-e53771</ispartof><rights>COPYRIGHT 2013 Public Library of Science</rights><rights>2013 Nasu et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License: https://creativecommons.org/licenses/by/4.0/ (the “License”), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><rights>2013 Nasu et al 2013 Nasu et al</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c692t-48dbd8cc50ea4bde2dc09075e758d1785bb4fd2e0be8ab278ac7e5948dbb0a6f3</citedby><cites>FETCH-LOGICAL-c692t-48dbd8cc50ea4bde2dc09075e758d1785bb4fd2e0be8ab278ac7e5948dbb0a6f3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC3561398/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC3561398/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,727,780,784,864,885,2102,2928,23866,27924,27925,53791,53793,79600,79601</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/23382851$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><contributor>Hu, Guang</contributor><creatorcontrib>Nasu, Akira</creatorcontrib><creatorcontrib>Ikeya, Makoto</creatorcontrib><creatorcontrib>Yamamoto, Takuya</creatorcontrib><creatorcontrib>Watanabe, Akira</creatorcontrib><creatorcontrib>Jin, Yonghui</creatorcontrib><creatorcontrib>Matsumoto, Yoshihisa</creatorcontrib><creatorcontrib>Hayakawa, Kazuo</creatorcontrib><creatorcontrib>Amano, Naoki</creatorcontrib><creatorcontrib>Sato, Shingo</creatorcontrib><creatorcontrib>Osafune, Kenji</creatorcontrib><creatorcontrib>Aoyama, Tomoki</creatorcontrib><creatorcontrib>Nakamura, Takashi</creatorcontrib><creatorcontrib>Kato, Tomohisa</creatorcontrib><creatorcontrib>Toguchida, Junya</creatorcontrib><title>Genetically matched human iPS cells reveal that propensity for cartilage and bone differentiation differs with clones, not cell type of origin</title><title>PloS one</title><addtitle>PLoS One</addtitle><description>For regenerative therapy using induced pluripotent stem cell (iPSC) technology, cell type of origin to be reprogrammed should be chosen based on accessibility and reprogramming efficiency. Some studies report that iPSCs exhibited a preference for differentiation into their original cell lineages, while others did not. Therefore, the type of cell which is most appropriate as a source for iPSCs needs to be clarified.
Genetically matched human iPSCs from different origins were generated using bone marrow stromal cells (BMSCs) and dermal fibroblasts (DFs) of the same donor, and global gene expression profile, DNA methylation status, and differentiation properties into the chondrogenic and osteogenic lineage of each clone were analyzed. Although genome-wide profiling of DNA methylation suggested tissue memory in iPSCs, genes expressed differentially in BMSCs and DFs were equally silenced in our bona fide iPSCs. After cell-autonomous and induced differentiation, each iPSC clone exhibited various differentiation properties, which did not correlate with cell-of-origin.
The reprogramming process may remove the difference between DFs and BMSCs at least for chondrogenic and osteogenic differentiation. Qualified and genetically matched human iPSC clone sets established in this study are valuable resources for further basic study of clonal differences.</description><subject>Analysis</subject><subject>Animal behavior</subject><subject>Biocompatibility</subject><subject>Biology</subject><subject>Biomedical materials</subject><subject>Bone Development - genetics</subject><subject>Bone marrow</subject><subject>Bone marrow transplantation</subject><subject>Cartilage</subject><subject>Cartilage - cytology</subject><subject>Cartilage - growth & development</subject><subject>Cell Differentiation</subject><subject>Cell growth</subject><subject>Cell Lineage</subject><subject>Clonal Evolution - genetics</subject><subject>Cloning</subject><subject>Deoxyribonucleic acid</subject><subject>Differentiation</subject><subject>DNA</subject><subject>DNA fingerprinting</subject><subject>DNA methylation</subject><subject>DNA Methylation - genetics</subject><subject>Efficiency</subject><subject>Fibroblasts</subject><subject>Fibroblasts - cytology</subject><subject>Gene expression</subject><subject>Gene Expression Regulation, Developmental</subject><subject>Genes</subject><subject>Genomes</subject><subject>Genomics</subject><subject>Humans</subject><subject>Induced Pluripotent Stem Cells - cytology</subject><subject>Medicine</subject><subject>Mesenchymal stem cells</subject><subject>Mesenchymal Stromal Cells - cytology</subject><subject>Methylation</subject><subject>Pluripotency</subject><subject>Science</subject><subject>Skin</subject><subject>Stem cell transplantation</subject><subject>Stem cells</subject><subject>Stromal cells</subject><subject>Surgery</subject><subject>Tissue 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matched human iPS cells reveal that propensity for cartilage and bone differentiation differs with clones, not cell type of origin</title><author>Nasu, Akira ; Ikeya, Makoto ; Yamamoto, Takuya ; Watanabe, Akira ; Jin, Yonghui ; Matsumoto, Yoshihisa ; Hayakawa, Kazuo ; Amano, Naoki ; Sato, Shingo ; Osafune, Kenji ; Aoyama, Tomoki ; Nakamura, Takashi ; Kato, Tomohisa ; Toguchida, Junya</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c692t-48dbd8cc50ea4bde2dc09075e758d1785bb4fd2e0be8ab278ac7e5948dbb0a6f3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2013</creationdate><topic>Analysis</topic><topic>Animal behavior</topic><topic>Biocompatibility</topic><topic>Biology</topic><topic>Biomedical materials</topic><topic>Bone Development - genetics</topic><topic>Bone marrow</topic><topic>Bone marrow transplantation</topic><topic>Cartilage</topic><topic>Cartilage - cytology</topic><topic>Cartilage - growth 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Tomohisa</au><au>Toguchida, Junya</au><au>Hu, Guang</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Genetically matched human iPS cells reveal that propensity for cartilage and bone differentiation differs with clones, not cell type of origin</atitle><jtitle>PloS one</jtitle><addtitle>PLoS One</addtitle><date>2013-01-31</date><risdate>2013</risdate><volume>8</volume><issue>1</issue><spage>e53771</spage><epage>e53771</epage><pages>e53771-e53771</pages><issn>1932-6203</issn><eissn>1932-6203</eissn><abstract>For regenerative therapy using induced pluripotent stem cell (iPSC) technology, cell type of origin to be reprogrammed should be chosen based on accessibility and reprogramming efficiency. Some studies report that iPSCs exhibited a preference for differentiation into their original cell lineages, while others did not. Therefore, the type of cell which is most appropriate as a source for iPSCs needs to be clarified.
Genetically matched human iPSCs from different origins were generated using bone marrow stromal cells (BMSCs) and dermal fibroblasts (DFs) of the same donor, and global gene expression profile, DNA methylation status, and differentiation properties into the chondrogenic and osteogenic lineage of each clone were analyzed. Although genome-wide profiling of DNA methylation suggested tissue memory in iPSCs, genes expressed differentially in BMSCs and DFs were equally silenced in our bona fide iPSCs. After cell-autonomous and induced differentiation, each iPSC clone exhibited various differentiation properties, which did not correlate with cell-of-origin.
The reprogramming process may remove the difference between DFs and BMSCs at least for chondrogenic and osteogenic differentiation. Qualified and genetically matched human iPSC clone sets established in this study are valuable resources for further basic study of clonal differences.</abstract><cop>United States</cop><pub>Public Library of Science</pub><pmid>23382851</pmid><doi>10.1371/journal.pone.0053771</doi><tpages>e53771</tpages><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 1932-6203 |
| ispartof | PloS one, 2013-01, Vol.8 (1), p.e53771-e53771 |
| issn | 1932-6203 1932-6203 |
| language | eng |
| recordid | cdi_plos_journals_1327941529 |
| source | MEDLINE; DOAJ Directory of Open Access Journals; Public Library of Science (PLoS) Journals Open Access; EZB-FREE-00999 freely available EZB journals; PubMed Central; Free Full-Text Journals in Chemistry |
| subjects | Analysis Animal behavior Biocompatibility Biology Biomedical materials Bone Development - genetics Bone marrow Bone marrow transplantation Cartilage Cartilage - cytology Cartilage - growth & development Cell Differentiation Cell growth Cell Lineage Clonal Evolution - genetics Cloning Deoxyribonucleic acid Differentiation DNA DNA fingerprinting DNA methylation DNA Methylation - genetics Efficiency Fibroblasts Fibroblasts - cytology Gene expression Gene Expression Regulation, Developmental Genes Genomes Genomics Humans Induced Pluripotent Stem Cells - cytology Medicine Mesenchymal stem cells Mesenchymal Stromal Cells - cytology Methylation Pluripotency Science Skin Stem cell transplantation Stem cells Stromal cells Surgery Tissue engineering |
| title | Genetically matched human iPS cells reveal that propensity for cartilage and bone differentiation differs with clones, not cell type of origin |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-07T10%3A56%3A49IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-gale_plos_&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Genetically%20matched%20human%20iPS%20cells%20reveal%20that%20propensity%20for%20cartilage%20and%20bone%20differentiation%20differs%20with%20clones,%20not%20cell%20type%20of%20origin&rft.jtitle=PloS%20one&rft.au=Nasu,%20Akira&rft.date=2013-01-31&rft.volume=8&rft.issue=1&rft.spage=e53771&rft.epage=e53771&rft.pages=e53771-e53771&rft.issn=1932-6203&rft.eissn=1932-6203&rft_id=info:doi/10.1371/journal.pone.0053771&rft_dat=%3Cgale_plos_%3EA477926888%3C/gale_plos_%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=1327941529&rft_id=info:pmid/23382851&rft_galeid=A477926888&rft_doaj_id=oai_doaj_org_article_df60f45ec31e4744a543588258eb64f3&rfr_iscdi=true |