Engineering foot-and-mouth disease viruses with improved growth properties for vaccine development
No licensed vaccine is currently available against serotype A foot-and-mouth disease (FMD) in China, despite the isolation of A/WH/CHA/09 in 2009, partly because this strain does not replicate well in baby hamster kidney (BHK) cells. A novel plasmid-based reverse genetics system was used to construc...
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| Veröffentlicht in: | PloS one 2013-01, Vol.8 (1), p.e55228-e55228 |
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| creator | Zheng, Haixue Guo, Jianhong Jin, Ye Yang, Fan He, Jijun Lv, Lv Zhang, Kesan Wu, Qiong Liu, Xiangtao Cai, Xuepeng |
| description | No licensed vaccine is currently available against serotype A foot-and-mouth disease (FMD) in China, despite the isolation of A/WH/CHA/09 in 2009, partly because this strain does not replicate well in baby hamster kidney (BHK) cells.
A novel plasmid-based reverse genetics system was used to construct a chimeric strain by replacing the P1 gene in the vaccine strain O/CHA/99 with that from the epidemic stain A/WH/CHA/09. The chimeric virus displayed growth kinetics similar to those of O/CHA/99 and was selected for use as a candidate vaccine strain after 12 passages in BHK cells. Cattle were vaccinated with the inactivated vaccine and humoral immune responses were induced in most of the animals on day 7. A challenge infection with A/WH/CHA/09 on day 28 indicated that the group given a 4-µg dose was fully protected and neither developed viremia nor seroconverted to a 3ABC antigen.
Our data demonstrate that the chimeric virus not only propagates well in BHK cells and has excellent antigenic matching against serotype A FMD, but is also a potential marker vaccine to distinguish infection from vaccination. These results suggest that reverse genetics technology is a useful tool for engineering vaccines for the prevention and control of FMD. |
| doi_str_mv | 10.1371/journal.pone.0055228 |
| format | Article |
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A novel plasmid-based reverse genetics system was used to construct a chimeric strain by replacing the P1 gene in the vaccine strain O/CHA/99 with that from the epidemic stain A/WH/CHA/09. The chimeric virus displayed growth kinetics similar to those of O/CHA/99 and was selected for use as a candidate vaccine strain after 12 passages in BHK cells. Cattle were vaccinated with the inactivated vaccine and humoral immune responses were induced in most of the animals on day 7. A challenge infection with A/WH/CHA/09 on day 28 indicated that the group given a 4-µg dose was fully protected and neither developed viremia nor seroconverted to a 3ABC antigen.
Our data demonstrate that the chimeric virus not only propagates well in BHK cells and has excellent antigenic matching against serotype A FMD, but is also a potential marker vaccine to distinguish infection from vaccination. These results suggest that reverse genetics technology is a useful tool for engineering vaccines for the prevention and control of FMD.</description><identifier>ISSN: 1932-6203</identifier><identifier>EISSN: 1932-6203</identifier><identifier>DOI: 10.1371/journal.pone.0055228</identifier><identifier>PMID: 23372840</identifier><language>eng</language><publisher>United States: Public Library of Science</publisher><subject><![CDATA[Animals ; Antibodies, Neutralizing - blood ; Antibodies, Neutralizing - immunology ; Antibodies, Viral - blood ; Antibodies, Viral - immunology ; Antigens ; Beef cattle ; Biology ; Bovidae ; Cattle ; Cattle Diseases - immunology ; Cattle Diseases - prevention & control ; Cell Line ; Cricetinae ; Engineering ; Epidemics ; Foot & mouth disease ; Foot-and-Mouth Disease - immunology ; Foot-and-Mouth Disease - prevention & control ; Foot-and-Mouth Disease Virus - genetics ; Foot-and-Mouth Disease Virus - growth & development ; Foot-and-Mouth Disease Virus - immunology ; Gene Order ; Genetics ; Growth ; Growth kinetics ; Hamsters ; Health aspects ; Immune response (humoral) ; Infections ; Kinetics ; Medicine ; Mice ; p1 gene ; Plasmids - genetics ; RNA, Viral - genetics ; Vaccination ; Vaccine development ; Vaccines ; Vaccines, Inactivated - administration & dosage ; Vaccines, Inactivated - genetics ; Vaccines, Inactivated - immunology ; Viral Vaccines - administration & dosage ; Viral Vaccines - genetics ; Viral Vaccines - immunology ; Viremia ; Virus diseases ; Viruses]]></subject><ispartof>PloS one, 2013-01, Vol.8 (1), p.e55228-e55228</ispartof><rights>COPYRIGHT 2013 Public Library of Science</rights><rights>2013 Zheng et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License: https://creativecommons.org/licenses/by/4.0/ (the “License”), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><rights>2013 Zheng et al 2013 Zheng et al</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c692t-953dd5deb74770e33053c47ee27b721270070ef719ed6b696dd27dff7483569f3</citedby><cites>FETCH-LOGICAL-c692t-953dd5deb74770e33053c47ee27b721270070ef719ed6b696dd27dff7483569f3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC3555929/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC3555929/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,723,776,780,860,881,2095,2914,23846,27903,27904,53770,53772,79347,79348</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/23372840$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><contributor>Thiel, Volker</contributor><creatorcontrib>Zheng, Haixue</creatorcontrib><creatorcontrib>Guo, Jianhong</creatorcontrib><creatorcontrib>Jin, Ye</creatorcontrib><creatorcontrib>Yang, Fan</creatorcontrib><creatorcontrib>He, Jijun</creatorcontrib><creatorcontrib>Lv, Lv</creatorcontrib><creatorcontrib>Zhang, Kesan</creatorcontrib><creatorcontrib>Wu, Qiong</creatorcontrib><creatorcontrib>Liu, Xiangtao</creatorcontrib><creatorcontrib>Cai, Xuepeng</creatorcontrib><title>Engineering foot-and-mouth disease viruses with improved growth properties for vaccine development</title><title>PloS one</title><addtitle>PLoS One</addtitle><description>No licensed vaccine is currently available against serotype A foot-and-mouth disease (FMD) in China, despite the isolation of A/WH/CHA/09 in 2009, partly because this strain does not replicate well in baby hamster kidney (BHK) cells.
A novel plasmid-based reverse genetics system was used to construct a chimeric strain by replacing the P1 gene in the vaccine strain O/CHA/99 with that from the epidemic stain A/WH/CHA/09. The chimeric virus displayed growth kinetics similar to those of O/CHA/99 and was selected for use as a candidate vaccine strain after 12 passages in BHK cells. Cattle were vaccinated with the inactivated vaccine and humoral immune responses were induced in most of the animals on day 7. A challenge infection with A/WH/CHA/09 on day 28 indicated that the group given a 4-µg dose was fully protected and neither developed viremia nor seroconverted to a 3ABC antigen.
Our data demonstrate that the chimeric virus not only propagates well in BHK cells and has excellent antigenic matching against serotype A FMD, but is also a potential marker vaccine to distinguish infection from vaccination. These results suggest that reverse genetics technology is a useful tool for engineering vaccines for the prevention and control of FMD.</description><subject>Animals</subject><subject>Antibodies, Neutralizing - blood</subject><subject>Antibodies, Neutralizing - immunology</subject><subject>Antibodies, Viral - blood</subject><subject>Antibodies, Viral - immunology</subject><subject>Antigens</subject><subject>Beef cattle</subject><subject>Biology</subject><subject>Bovidae</subject><subject>Cattle</subject><subject>Cattle Diseases - immunology</subject><subject>Cattle Diseases - prevention & control</subject><subject>Cell Line</subject><subject>Cricetinae</subject><subject>Engineering</subject><subject>Epidemics</subject><subject>Foot & mouth disease</subject><subject>Foot-and-Mouth Disease - immunology</subject><subject>Foot-and-Mouth Disease - prevention & control</subject><subject>Foot-and-Mouth Disease Virus - genetics</subject><subject>Foot-and-Mouth Disease Virus - growth & development</subject><subject>Foot-and-Mouth Disease Virus - 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Academic</collection><collection>PubMed Central (Full Participant titles)</collection><collection>DOAJ Directory of Open Access Journals</collection><jtitle>PloS one</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Zheng, Haixue</au><au>Guo, Jianhong</au><au>Jin, Ye</au><au>Yang, Fan</au><au>He, Jijun</au><au>Lv, Lv</au><au>Zhang, Kesan</au><au>Wu, Qiong</au><au>Liu, Xiangtao</au><au>Cai, Xuepeng</au><au>Thiel, Volker</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Engineering foot-and-mouth disease viruses with improved growth properties for vaccine development</atitle><jtitle>PloS one</jtitle><addtitle>PLoS One</addtitle><date>2013-01-25</date><risdate>2013</risdate><volume>8</volume><issue>1</issue><spage>e55228</spage><epage>e55228</epage><pages>e55228-e55228</pages><issn>1932-6203</issn><eissn>1932-6203</eissn><abstract>No licensed vaccine is currently available against serotype A foot-and-mouth disease (FMD) in China, despite the isolation of A/WH/CHA/09 in 2009, partly because this strain does not replicate well in baby hamster kidney (BHK) cells.
A novel plasmid-based reverse genetics system was used to construct a chimeric strain by replacing the P1 gene in the vaccine strain O/CHA/99 with that from the epidemic stain A/WH/CHA/09. The chimeric virus displayed growth kinetics similar to those of O/CHA/99 and was selected for use as a candidate vaccine strain after 12 passages in BHK cells. Cattle were vaccinated with the inactivated vaccine and humoral immune responses were induced in most of the animals on day 7. A challenge infection with A/WH/CHA/09 on day 28 indicated that the group given a 4-µg dose was fully protected and neither developed viremia nor seroconverted to a 3ABC antigen.
Our data demonstrate that the chimeric virus not only propagates well in BHK cells and has excellent antigenic matching against serotype A FMD, but is also a potential marker vaccine to distinguish infection from vaccination. These results suggest that reverse genetics technology is a useful tool for engineering vaccines for the prevention and control of FMD.</abstract><cop>United States</cop><pub>Public Library of Science</pub><pmid>23372840</pmid><doi>10.1371/journal.pone.0055228</doi><tpages>e55228</tpages><oa>free_for_read</oa></addata></record> |
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| issn | 1932-6203 1932-6203 |
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| source | MEDLINE; DOAJ Directory of Open Access Journals; Public Library of Science (PLoS); EZB-FREE-00999 freely available EZB journals; PubMed Central; Free Full-Text Journals in Chemistry |
| subjects | Animals Antibodies, Neutralizing - blood Antibodies, Neutralizing - immunology Antibodies, Viral - blood Antibodies, Viral - immunology Antigens Beef cattle Biology Bovidae Cattle Cattle Diseases - immunology Cattle Diseases - prevention & control Cell Line Cricetinae Engineering Epidemics Foot & mouth disease Foot-and-Mouth Disease - immunology Foot-and-Mouth Disease - prevention & control Foot-and-Mouth Disease Virus - genetics Foot-and-Mouth Disease Virus - growth & development Foot-and-Mouth Disease Virus - immunology Gene Order Genetics Growth Growth kinetics Hamsters Health aspects Immune response (humoral) Infections Kinetics Medicine Mice p1 gene Plasmids - genetics RNA, Viral - genetics Vaccination Vaccine development Vaccines Vaccines, Inactivated - administration & dosage Vaccines, Inactivated - genetics Vaccines, Inactivated - immunology Viral Vaccines - administration & dosage Viral Vaccines - genetics Viral Vaccines - immunology Viremia Virus diseases Viruses |
| title | Engineering foot-and-mouth disease viruses with improved growth properties for vaccine development |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-22T08%3A43%3A31IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-gale_plos_&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Engineering%20foot-and-mouth%20disease%20viruses%20with%20improved%20growth%20properties%20for%20vaccine%20development&rft.jtitle=PloS%20one&rft.au=Zheng,%20Haixue&rft.date=2013-01-25&rft.volume=8&rft.issue=1&rft.spage=e55228&rft.epage=e55228&rft.pages=e55228-e55228&rft.issn=1932-6203&rft.eissn=1932-6203&rft_id=info:doi/10.1371/journal.pone.0055228&rft_dat=%3Cgale_plos_%3EA478883660%3C/gale_plos_%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=1327941232&rft_id=info:pmid/23372840&rft_galeid=A478883660&rft_doaj_id=oai_doaj_org_article_9f19cfc07a6f45e48aee562b4ec372aa&rfr_iscdi=true |