Molecular viability testing of bacterial pathogens from a complex human sample matrix

Assays for bacterial ribosomal RNA precursors (pre-rRNA) have been shown to distinguish viable from inactivated bacterial cells in drinking water samples. Because the synthesis of pre-rRNA is rapidly induced by nutritional stimulation, viable bacteria can be distinguished from inactivated cells and...

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Veröffentlicht in:	PloS one 2013-01, Vol.8 (1), p.e54886
Hauptverfasser:	Weigel, Kris M, Jones, Kelly L, Do, Julie S, Melton Witt, Jody, Chung, Jae-Hyun, Valcke, Christian, Cangelosi, Gerard A
Format:	Artikel
Sprache:	eng
Schlagworte:	Acinetobacter Acinetobacter baumannii Acinetobacter baumannii - genetics Acinetobacter baumannii - isolation & purification Automation Bacillus subtilis Bacteria Biodiversity Biology Cell culture Colony Count, Microbial Culture Media Deoxyribonucleic acid DNA DNA, Bacterial - blood Drinking water E coli Escherichia coli Genetic aspects Health sciences Humans Laboratories Medicine Microbial Viability Microbiological analysis Microbiology Mycobacterium tuberculosis - genetics Mycobacterium tuberculosis - isolation & purification Nucleic acids Nutrients Occupational health Pathogenic bacteria Pathogens Phylogeny Polymerase chain reaction Properties Pseudomonas aeruginosa Pseudomonas aeruginosa - genetics Pseudomonas aeruginosa - isolation & purification Replenishment Resolution Ribonucleic acid Ribosomal RNA RNA RNA Precursors - blood RNA, Bacterial - blood RNA-directed DNA polymerase rRNA Sampling methods Serum - microbiology Species Species diversity Staphylococcus aureus Staphylococcus aureus - genetics Staphylococcus aureus - isolation & purification Staphylococcus infections Stimulation Tuberculosis Viability Virulence (Microbiology) Water analysis Water sampling
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