Method for quantitative study of airway functional microanatomy using micro-optical coherence tomography
We demonstrate the use of a high resolution form of optical coherence tomography, termed micro-OCT (μOCT), for investigating the functional microanatomy of airway epithelia. μOCT captures several key parameters governing the function of the airway surface (airway surface liquid depth, periciliary li...
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| Veröffentlicht in: | PloS one 2013-01, Vol.8 (1), p.e54473-e54473 |
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| creator | Liu, Linbo Chu, Kengyeh K Houser, Grace H Diephuis, Bradford J Li, Yao Wilsterman, Eric J Shastry, Suresh Dierksen, Gregory Birket, Susan E Mazur, Marina Byan-Parker, Suzanne Grizzle, William E Sorscher, Eric J Rowe, Steven M Tearney, Guillermo J |
| description | We demonstrate the use of a high resolution form of optical coherence tomography, termed micro-OCT (μOCT), for investigating the functional microanatomy of airway epithelia. μOCT captures several key parameters governing the function of the airway surface (airway surface liquid depth, periciliary liquid depth, ciliary function including beat frequency, and mucociliary transport rate) from the same series of images and without exogenous particles or labels, enabling non-invasive study of dynamic phenomena. Additionally, the high resolution of μOCT reveals distinguishable phases of the ciliary stroke pattern and glandular extrusion. Images and functional measurements from primary human bronchial epithelial cell cultures and excised tissue are presented and compared with measurements using existing gold standard methods. Active secretion from mucus glands in tissue, a key parameter of epithelial function, was also observed and quantified. |
| doi_str_mv | 10.1371/journal.pone.0054473 |
| format | Article |
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Additionally, the high resolution of μOCT reveals distinguishable phases of the ciliary stroke pattern and glandular extrusion. Images and functional measurements from primary human bronchial epithelial cell cultures and excised tissue are presented and compared with measurements using existing gold standard methods. Active secretion from mucus glands in tissue, a key parameter of epithelial function, was also observed and quantified.</description><identifier>ISSN: 1932-6203</identifier><identifier>EISSN: 1932-6203</identifier><identifier>DOI: 10.1371/journal.pone.0054473</identifier><identifier>PMID: 23372732</identifier><language>eng</language><publisher>United States: Public Library of Science</publisher><subject>Animals ; Biology ; Bronchi - physiology ; Bronchi - ultrastructure ; Chronic obstructive pulmonary disease ; Cilia - physiology ; Cilia - ultrastructure ; Coherence ; Cystic fibrosis ; Dermatology ; Engineering ; Epithelial cells ; Epithelial Cells - physiology ; Epithelial Cells - ultrastructure ; Extrusion ; Glands ; Health sciences ; High resolution ; Hospitals ; Humans ; Medical imaging ; Medical schools ; Medicine ; Methods ; Microscopy ; Mucociliary Clearance - physiology ; Mucus ; Mucus - metabolism ; Optical Coherence Tomography ; Pathology ; Physiology ; Primary Cell Culture ; Quantitative research ; Respiratory Mucosa - physiology ; Respiratory Mucosa - ultrastructure ; Respiratory tract ; Secretion ; Swine ; Tomography ; Tomography, Optical Coherence - instrumentation ; Tomography, Optical Coherence - methods</subject><ispartof>PloS one, 2013-01, Vol.8 (1), p.e54473-e54473</ispartof><rights>COPYRIGHT 2013 Public Library of Science</rights><rights>2013 Liu et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License: https://creativecommons.org/licenses/by/4.0/ (the “License”), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><rights>2013 Liu et al 2013 Liu et al</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c758t-68fbd01201ea915e73ab57eac1fc3b105895b3e6fd26063c8ce8db01004d9f6e3</citedby><cites>FETCH-LOGICAL-c758t-68fbd01201ea915e73ab57eac1fc3b105895b3e6fd26063c8ce8db01004d9f6e3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC3553101/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC3553101/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,724,777,781,861,882,2096,2915,23847,27905,27906,53772,53774,79349,79350</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/23372732$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><contributor>Myerburg, Michael</contributor><creatorcontrib>Liu, Linbo</creatorcontrib><creatorcontrib>Chu, Kengyeh K</creatorcontrib><creatorcontrib>Houser, Grace H</creatorcontrib><creatorcontrib>Diephuis, Bradford J</creatorcontrib><creatorcontrib>Li, Yao</creatorcontrib><creatorcontrib>Wilsterman, Eric J</creatorcontrib><creatorcontrib>Shastry, Suresh</creatorcontrib><creatorcontrib>Dierksen, Gregory</creatorcontrib><creatorcontrib>Birket, Susan E</creatorcontrib><creatorcontrib>Mazur, Marina</creatorcontrib><creatorcontrib>Byan-Parker, Suzanne</creatorcontrib><creatorcontrib>Grizzle, William E</creatorcontrib><creatorcontrib>Sorscher, Eric J</creatorcontrib><creatorcontrib>Rowe, Steven M</creatorcontrib><creatorcontrib>Tearney, Guillermo J</creatorcontrib><title>Method for quantitative study of airway functional microanatomy using micro-optical coherence tomography</title><title>PloS one</title><addtitle>PLoS One</addtitle><description>We demonstrate the use of a high resolution form of optical coherence tomography, termed micro-OCT (μOCT), for investigating the functional microanatomy of airway epithelia. μOCT captures several key parameters governing the function of the airway surface (airway surface liquid depth, periciliary liquid depth, ciliary function including beat frequency, and mucociliary transport rate) from the same series of images and without exogenous particles or labels, enabling non-invasive study of dynamic phenomena. Additionally, the high resolution of μOCT reveals distinguishable phases of the ciliary stroke pattern and glandular extrusion. Images and functional measurements from primary human bronchial epithelial cell cultures and excised tissue are presented and compared with measurements using existing gold standard methods. Active secretion from mucus glands in tissue, a key parameter of epithelial function, was also observed and quantified.</description><subject>Animals</subject><subject>Biology</subject><subject>Bronchi - physiology</subject><subject>Bronchi - ultrastructure</subject><subject>Chronic obstructive pulmonary disease</subject><subject>Cilia - physiology</subject><subject>Cilia - ultrastructure</subject><subject>Coherence</subject><subject>Cystic fibrosis</subject><subject>Dermatology</subject><subject>Engineering</subject><subject>Epithelial cells</subject><subject>Epithelial Cells - physiology</subject><subject>Epithelial Cells - ultrastructure</subject><subject>Extrusion</subject><subject>Glands</subject><subject>Health sciences</subject><subject>High resolution</subject><subject>Hospitals</subject><subject>Humans</subject><subject>Medical imaging</subject><subject>Medical schools</subject><subject>Medicine</subject><subject>Methods</subject><subject>Microscopy</subject><subject>Mucociliary Clearance - physiology</subject><subject>Mucus</subject><subject>Mucus - metabolism</subject><subject>Optical Coherence Tomography</subject><subject>Pathology</subject><subject>Physiology</subject><subject>Primary Cell Culture</subject><subject>Quantitative research</subject><subject>Respiratory Mucosa - physiology</subject><subject>Respiratory Mucosa - ultrastructure</subject><subject>Respiratory tract</subject><subject>Secretion</subject><subject>Swine</subject><subject>Tomography</subject><subject>Tomography, Optical Coherence - instrumentation</subject><subject>Tomography, Optical Coherence - methods</subject><issn>1932-6203</issn><issn>1932-6203</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2013</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><sourceid>DOA</sourceid><recordid>eNqNk0tv1DAQxyMEoqXwDRBEQkJw2MWPxHYuSFXFY6WiSryuluOME6-y8dZ2Cvvt8bJptUE9IB9sjX_zH894JsueY7TElON3azf6QfXLrRtgiVBZFJw-yE5xRcmCEUQfHp1PsichrBNEBWOPsxNCKSecktOs-wKxc01unM-vRzVEG1W0N5CHODa73JlcWf9L7XIzDjpalyLmG6u9U4OKbrPLx2CH9mBauG20OgHadeBh0JAnxLVebbvd0-yRUX2AZ9N-lv34-OH7xefF5dWn1cX55ULzUsQFE6ZuECYIg6pwCZyquuSgNDaa1hiVoiprCsw0hCFGtdAgmhphhIqmMgzoWfbyoLvtXZBTkYLElHBBSi5oIlYHonFqLbfebpTfSaes_GtwvpXKp0R6kAwDYlVDKqTKohRFVSGkgDS6MICrAiet91O0sd5Ao2GIXvUz0fnNYDvZuhtJy5JitBd4Mwl4dz1CiHJjg4a-VwO4Mb2bCMopRYQl9NU_6P3ZTVSrUgJ2MC7F1XtReV5wgQvBC5Go5T1UWg2kr0wdZWyyzxzezhwSE-F3bNUYglx9-_r_7NXPOfv6iO1A9bELrh_3rRbmYHEAU6OF4MHcFRkjuR-I22rI_UDIaSCS24vjD7pzup0A-gdJjAdV</recordid><startdate>20130123</startdate><enddate>20130123</enddate><creator>Liu, Linbo</creator><creator>Chu, Kengyeh K</creator><creator>Houser, Grace H</creator><creator>Diephuis, Bradford J</creator><creator>Li, Yao</creator><creator>Wilsterman, Eric J</creator><creator>Shastry, Suresh</creator><creator>Dierksen, Gregory</creator><creator>Birket, Susan E</creator><creator>Mazur, Marina</creator><creator>Byan-Parker, Suzanne</creator><creator>Grizzle, William E</creator><creator>Sorscher, Eric J</creator><creator>Rowe, Steven M</creator><creator>Tearney, Guillermo J</creator><general>Public Library of Science</general><general>Public Library of Science (PLoS)</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>IOV</scope><scope>ISR</scope><scope>3V.</scope><scope>7QG</scope><scope>7QL</scope><scope>7QO</scope><scope>7RV</scope><scope>7SN</scope><scope>7SS</scope><scope>7T5</scope><scope>7TG</scope><scope>7TM</scope><scope>7U9</scope><scope>7X2</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>8AO</scope><scope>8C1</scope><scope>8FD</scope><scope>8FE</scope><scope>8FG</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABJCF</scope><scope>ABUWG</scope><scope>AEUYN</scope><scope>AFKRA</scope><scope>ARAPS</scope><scope>ATCPS</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BGLVJ</scope><scope>BHPHI</scope><scope>C1K</scope><scope>CCPQU</scope><scope>D1I</scope><scope>DWQXO</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>H94</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>KB.</scope><scope>KB0</scope><scope>KL.</scope><scope>L6V</scope><scope>LK8</scope><scope>M0K</scope><scope>M0S</scope><scope>M1P</scope><scope>M7N</scope><scope>M7P</scope><scope>M7S</scope><scope>NAPCQ</scope><scope>P5Z</scope><scope>P62</scope><scope>P64</scope><scope>PATMY</scope><scope>PDBOC</scope><scope>PIMPY</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>PTHSS</scope><scope>PYCSY</scope><scope>RC3</scope><scope>7X8</scope><scope>5PM</scope><scope>DOA</scope></search><sort><creationdate>20130123</creationdate><title>Method for quantitative study of airway functional microanatomy using micro-optical coherence tomography</title><author>Liu, Linbo ; Chu, Kengyeh K ; Houser, Grace H ; Diephuis, Bradford J ; Li, Yao ; Wilsterman, Eric J ; Shastry, Suresh ; Dierksen, Gregory ; Birket, Susan E ; Mazur, Marina ; Byan-Parker, Suzanne ; Grizzle, William E ; Sorscher, Eric J ; Rowe, Steven M ; Tearney, Guillermo J</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c758t-68fbd01201ea915e73ab57eac1fc3b105895b3e6fd26063c8ce8db01004d9f6e3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2013</creationdate><topic>Animals</topic><topic>Biology</topic><topic>Bronchi - 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| subjects | Animals Biology Bronchi - physiology Bronchi - ultrastructure Chronic obstructive pulmonary disease Cilia - physiology Cilia - ultrastructure Coherence Cystic fibrosis Dermatology Engineering Epithelial cells Epithelial Cells - physiology Epithelial Cells - ultrastructure Extrusion Glands Health sciences High resolution Hospitals Humans Medical imaging Medical schools Medicine Methods Microscopy Mucociliary Clearance - physiology Mucus Mucus - metabolism Optical Coherence Tomography Pathology Physiology Primary Cell Culture Quantitative research Respiratory Mucosa - physiology Respiratory Mucosa - ultrastructure Respiratory tract Secretion Swine Tomography Tomography, Optical Coherence - instrumentation Tomography, Optical Coherence - methods |
| title | Method for quantitative study of airway functional microanatomy using micro-optical coherence tomography |
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