Regulation of wound healing by granulocyte-macrophage colony-stimulating factor after vocal fold injury
Vocal fold (VF) scarring remains a therapeutic challenge. Granulocyte-macrophage colony-stimulating factor (GM-CSF) facilitates epithelial wound healing, and recently, growth factor therapy has been applied to promote tissue repair. This study was undertaken to investigate the effect of GM-CSF on VF...
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| description | Vocal fold (VF) scarring remains a therapeutic challenge. Granulocyte-macrophage colony-stimulating factor (GM-CSF) facilitates epithelial wound healing, and recently, growth factor therapy has been applied to promote tissue repair. This study was undertaken to investigate the effect of GM-CSF on VF wound healing in vivo and in vitro.
VF scarring was induced in New Zealand white rabbits by direct injury. Immediately thereafter, either GM-CSF or PBS was injected into the VFs of rabbits. Endoscopic, histopathological, immunohistochemical, and biomechanical evaluations of VFs were performed at 3 months post-injury. Human vocal fold fibroblasts (hVFFs) were cultured with GM-CSF. Production of type I and III collagen was examined immunocytochemically, and the synthesis of elastin and hyaluronic acids was evaluated by ELISA. The mRNA levels of genes related to ECM components and ECM production-related growth factors, such as HGF and TGF-ß1, were examined by real time RT-PCR.
The GM-CSF-treated VFs showed reduced collagen deposition in comparison to the PBS-injected controls (P |
| doi_str_mv | 10.1371/journal.pone.0054256 |
| format | Article |
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VF scarring was induced in New Zealand white rabbits by direct injury. Immediately thereafter, either GM-CSF or PBS was injected into the VFs of rabbits. Endoscopic, histopathological, immunohistochemical, and biomechanical evaluations of VFs were performed at 3 months post-injury. Human vocal fold fibroblasts (hVFFs) were cultured with GM-CSF. Production of type I and III collagen was examined immunocytochemically, and the synthesis of elastin and hyaluronic acids was evaluated by ELISA. The mRNA levels of genes related to ECM components and ECM production-related growth factors, such as HGF and TGF-ß1, were examined by real time RT-PCR.
The GM-CSF-treated VFs showed reduced collagen deposition in comparison to the PBS-injected controls (P<0.05). Immunohistochemical staining revealed lower amounts of type I collagen and fibronectin in the GM-CSF-treated VFs (P<0.05 and P<0.01, respectively). Viscous and elastic shear moduli of VF samples were significantly lower in the GM-CSF group than in the PBS-injected group (P<0.001 and P<0.01, respectively). Mucosal waves in the GM-CSF group showed significant improvement when compared to the PBS group (P = 0.0446). GM-CSF inhibited TGF-β1-induced collagen synthesis by hVFFs (P<0.05) and the production of hyaluronic acids increased at 72 hours post-treatment (P<0.05). The expressions of HAS-2, tropoelastin, MMP-1, HGF, and c-Met mRNA were significantly increased by GM-CSF, although at different time points (P<0.05).
The present study shows that GM-CSF offers therapeutic potential for the remodeling of VF wounds and the promotion of VF regeneration.]]></description><identifier>ISSN: 1932-6203</identifier><identifier>EISSN: 1932-6203</identifier><identifier>DOI: 10.1371/journal.pone.0054256</identifier><identifier>PMID: 23372696</identifier><language>eng</language><publisher>United States: Public Library of Science</publisher><subject>Acids ; Animals ; Biology ; Biomechanics ; c-Met protein ; Cells, Cultured ; Collagen ; Collagen (type I) ; Collagen Type I - antagonists & inhibitors ; Collagen Type I - genetics ; Collagen Type I - metabolism ; Collagen Type III - genetics ; Collagen Type III - metabolism ; Colonies ; Colony-stimulating factor ; Cytokines ; Elastin ; Elastin - genetics ; Elastin - metabolism ; Enzyme-linked immunosorbent assay ; Experiments ; Extracellular matrix ; Extracellular Matrix - drug effects ; Extracellular Matrix - genetics ; Extracellular Matrix - metabolism ; Fibroblasts ; Fibroblasts - cytology ; Fibroblasts - drug effects ; Fibroblasts - metabolism ; Fibronectin ; Fibronectins ; Fibronectins - antagonists & inhibitors ; Fibronectins - genetics ; Fibronectins - metabolism ; Gene expression ; Gene Expression - drug effects ; Granulocyte-macrophage colony-stimulating factor ; Granulocyte-Macrophage Colony-Stimulating Factor - metabolism ; Granulocyte-Macrophage Colony-Stimulating Factor - pharmacology ; Granulocytes ; Growth factors ; Health aspects ; Hepatocyte Growth Factor - genetics ; Hepatocyte Growth Factor - metabolism ; Humans ; Hyaluronic Acid - biosynthesis ; Immunohistochemistry ; In vitro methods and tests ; In vivo methods and tests ; Injections, Intralesional ; Injuries ; Interstitial collagenase ; Macrophage colony stimulating factor ; Macrophages ; Matrix metalloproteinase ; Medicine ; mRNA ; Mucosa ; Otolaryngology ; Polymerase chain reaction ; Rabbits ; Regeneration ; RNA ; Scars ; Studies ; Surgery ; Synthesis ; Tissue engineering ; Transforming Growth Factor beta1 - genetics ; Transforming Growth Factor beta1 - metabolism ; Transforming growth factor-b1 ; Transforming growth factors ; Tropoelastin ; Vocal Cords - drug effects ; Vocal Cords - injuries ; Vocal Cords - metabolism ; Wound care ; Wound healing ; Wound Healing - drug effects ; Wound Healing - physiology</subject><ispartof>PloS one, 2013-01, Vol.8 (1), p.e54256</ispartof><rights>COPYRIGHT 2013 Public Library of Science</rights><rights>2013 Lim et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License: https://creativecommons.org/licenses/by/4.0/ (the “License”), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><rights>2013 Lim et al 2013 Lim et al</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c692t-e544a24640d1975adbda9676c9e6d3d7beff0b842bd9cf43818e03b1ecb0ab273</citedby><cites>FETCH-LOGICAL-c692t-e544a24640d1975adbda9676c9e6d3d7beff0b842bd9cf43818e03b1ecb0ab273</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC3556034/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC3556034/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,723,776,780,860,881,2096,2915,23845,27901,27902,53766,53768,79343,79344</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/23372696$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><contributor>Zhang, Luo</contributor><creatorcontrib>Lim, Jae-Yol</creatorcontrib><creatorcontrib>Choi, Byung Hyune</creatorcontrib><creatorcontrib>Lee, Songyi</creatorcontrib><creatorcontrib>Jang, Yun Ho</creatorcontrib><creatorcontrib>Choi, Jeong-Seok</creatorcontrib><creatorcontrib>Kim, Young-Mo</creatorcontrib><title>Regulation of wound healing by granulocyte-macrophage colony-stimulating factor after vocal fold injury</title><title>PloS one</title><addtitle>PLoS One</addtitle><description><![CDATA[Vocal fold (VF) scarring remains a therapeutic challenge. Granulocyte-macrophage colony-stimulating factor (GM-CSF) facilitates epithelial wound healing, and recently, growth factor therapy has been applied to promote tissue repair. This study was undertaken to investigate the effect of GM-CSF on VF wound healing in vivo and in vitro.
VF scarring was induced in New Zealand white rabbits by direct injury. Immediately thereafter, either GM-CSF or PBS was injected into the VFs of rabbits. Endoscopic, histopathological, immunohistochemical, and biomechanical evaluations of VFs were performed at 3 months post-injury. Human vocal fold fibroblasts (hVFFs) were cultured with GM-CSF. Production of type I and III collagen was examined immunocytochemically, and the synthesis of elastin and hyaluronic acids was evaluated by ELISA. The mRNA levels of genes related to ECM components and ECM production-related growth factors, such as HGF and TGF-ß1, were examined by real time RT-PCR.
The GM-CSF-treated VFs showed reduced collagen deposition in comparison to the PBS-injected controls (P<0.05). Immunohistochemical staining revealed lower amounts of type I collagen and fibronectin in the GM-CSF-treated VFs (P<0.05 and P<0.01, respectively). Viscous and elastic shear moduli of VF samples were significantly lower in the GM-CSF group than in the PBS-injected group (P<0.001 and P<0.01, respectively). Mucosal waves in the GM-CSF group showed significant improvement when compared to the PBS group (P = 0.0446). GM-CSF inhibited TGF-β1-induced collagen synthesis by hVFFs (P<0.05) and the production of hyaluronic acids increased at 72 hours post-treatment (P<0.05). The expressions of HAS-2, tropoelastin, MMP-1, HGF, and c-Met mRNA were significantly increased by GM-CSF, although at different time points (P<0.05).
The present study shows that GM-CSF offers therapeutic potential for the remodeling of VF wounds and the promotion of VF regeneration.]]></description><subject>Acids</subject><subject>Animals</subject><subject>Biology</subject><subject>Biomechanics</subject><subject>c-Met protein</subject><subject>Cells, Cultured</subject><subject>Collagen</subject><subject>Collagen (type I)</subject><subject>Collagen Type I - antagonists & inhibitors</subject><subject>Collagen Type I - genetics</subject><subject>Collagen Type I - metabolism</subject><subject>Collagen Type III - genetics</subject><subject>Collagen Type III - metabolism</subject><subject>Colonies</subject><subject>Colony-stimulating factor</subject><subject>Cytokines</subject><subject>Elastin</subject><subject>Elastin - genetics</subject><subject>Elastin - metabolism</subject><subject>Enzyme-linked immunosorbent assay</subject><subject>Experiments</subject><subject>Extracellular matrix</subject><subject>Extracellular Matrix - drug effects</subject><subject>Extracellular Matrix - genetics</subject><subject>Extracellular Matrix - metabolism</subject><subject>Fibroblasts</subject><subject>Fibroblasts - cytology</subject><subject>Fibroblasts - drug effects</subject><subject>Fibroblasts - metabolism</subject><subject>Fibronectin</subject><subject>Fibronectins</subject><subject>Fibronectins - antagonists & inhibitors</subject><subject>Fibronectins - genetics</subject><subject>Fibronectins - metabolism</subject><subject>Gene expression</subject><subject>Gene Expression - drug effects</subject><subject>Granulocyte-macrophage colony-stimulating factor</subject><subject>Granulocyte-Macrophage Colony-Stimulating Factor - metabolism</subject><subject>Granulocyte-Macrophage Colony-Stimulating Factor - pharmacology</subject><subject>Granulocytes</subject><subject>Growth factors</subject><subject>Health aspects</subject><subject>Hepatocyte Growth Factor - genetics</subject><subject>Hepatocyte Growth Factor - metabolism</subject><subject>Humans</subject><subject>Hyaluronic Acid - biosynthesis</subject><subject>Immunohistochemistry</subject><subject>In vitro methods and tests</subject><subject>In vivo methods and tests</subject><subject>Injections, Intralesional</subject><subject>Injuries</subject><subject>Interstitial collagenase</subject><subject>Macrophage colony stimulating factor</subject><subject>Macrophages</subject><subject>Matrix metalloproteinase</subject><subject>Medicine</subject><subject>mRNA</subject><subject>Mucosa</subject><subject>Otolaryngology</subject><subject>Polymerase chain reaction</subject><subject>Rabbits</subject><subject>Regeneration</subject><subject>RNA</subject><subject>Scars</subject><subject>Studies</subject><subject>Surgery</subject><subject>Synthesis</subject><subject>Tissue engineering</subject><subject>Transforming Growth Factor beta1 - genetics</subject><subject>Transforming Growth Factor beta1 - metabolism</subject><subject>Transforming growth factor-b1</subject><subject>Transforming growth factors</subject><subject>Tropoelastin</subject><subject>Vocal Cords - drug effects</subject><subject>Vocal Cords - injuries</subject><subject>Vocal Cords - metabolism</subject><subject>Wound care</subject><subject>Wound healing</subject><subject>Wound Healing - drug effects</subject><subject>Wound Healing - physiology</subject><issn>1932-6203</issn><issn>1932-6203</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2013</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>BENPR</sourceid><sourceid>DOA</sourceid><recordid>eNqNkl2L1DAUhoso7rr6D0QLguBFx6RJ0_ZGWBY_BhYW1o_bcJImnQxpMybp6vx7MzvdZQsKkouEk-d9z-HwZtlLjFaY1Pj91k1-BLvauVGtEKpoWbFH2SluSVmwEpHHD94n2bMQtgkiDWNPs5OSkLpkLTvN-mvVTxaicWPudP7LTWOXbxRYM_a52Oe9h3GyTu6jKgaQ3u020KtcOuvGfRGiGW7VCdYgo_M56Kh8fuMk2Fw72-Vm3E5-_zx7osEG9WK-z7Lvnz5-u_hSXF59Xl-cXxaStWUsVEUplJRR1OG2rqATHbSsZrJVrCNdLZTWSDS0FF0rNSUNbhQiAispEIiyJmfZ66PvzrrA5x0FjklZ123bVGUi1keic7DlO28G8HvuwPDbgvM9Bx-NtIoLCQgJQgDTlkrcQKNppXQrKGiC2kO3D3O3SQyqk2qMHuzCdPkzmg3v3Q0nVcUQocngzWzg3c9JhfiPkWeqhzSVGbVLZnIwQfJzWjdNQxhmiVr9hUqnU4ORKSXapPpC8G4hSExUv2MPUwh8_fX6_9mrH0v27QP2EKa4Cc5Oh5CFJUiPYMpVCF7p-81hxA8hv9sGP4SczyFPslcPt34vuks1-QPJyPn9</recordid><startdate>20130125</startdate><enddate>20130125</enddate><creator>Lim, Jae-Yol</creator><creator>Choi, Byung Hyune</creator><creator>Lee, Songyi</creator><creator>Jang, Yun Ho</creator><creator>Choi, Jeong-Seok</creator><creator>Kim, Young-Mo</creator><general>Public Library of Science</general><general>Public Library of Science (PLoS)</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>IOV</scope><scope>ISR</scope><scope>3V.</scope><scope>7QG</scope><scope>7QL</scope><scope>7QO</scope><scope>7RV</scope><scope>7SN</scope><scope>7SS</scope><scope>7T5</scope><scope>7TG</scope><scope>7TM</scope><scope>7U9</scope><scope>7X2</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>8AO</scope><scope>8C1</scope><scope>8FD</scope><scope>8FE</scope><scope>8FG</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABJCF</scope><scope>ABUWG</scope><scope>AEUYN</scope><scope>AFKRA</scope><scope>ARAPS</scope><scope>ATCPS</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BGLVJ</scope><scope>BHPHI</scope><scope>C1K</scope><scope>CCPQU</scope><scope>D1I</scope><scope>DWQXO</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>H94</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>KB.</scope><scope>KB0</scope><scope>KL.</scope><scope>L6V</scope><scope>LK8</scope><scope>M0K</scope><scope>M0S</scope><scope>M1P</scope><scope>M7N</scope><scope>M7P</scope><scope>M7S</scope><scope>NAPCQ</scope><scope>P5Z</scope><scope>P62</scope><scope>P64</scope><scope>PATMY</scope><scope>PDBOC</scope><scope>PIMPY</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PTHSS</scope><scope>PYCSY</scope><scope>RC3</scope><scope>5PM</scope><scope>DOA</scope></search><sort><creationdate>20130125</creationdate><title>Regulation of wound healing by granulocyte-macrophage colony-stimulating factor after vocal fold injury</title><author>Lim, Jae-Yol ; Choi, Byung Hyune ; Lee, Songyi ; Jang, Yun Ho ; Choi, Jeong-Seok ; Kim, Young-Mo</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c692t-e544a24640d1975adbda9676c9e6d3d7beff0b842bd9cf43818e03b1ecb0ab273</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2013</creationdate><topic>Acids</topic><topic>Animals</topic><topic>Biology</topic><topic>Biomechanics</topic><topic>c-Met protein</topic><topic>Cells, Cultured</topic><topic>Collagen</topic><topic>Collagen (type I)</topic><topic>Collagen Type I - antagonists & inhibitors</topic><topic>Collagen Type I - genetics</topic><topic>Collagen Type I - metabolism</topic><topic>Collagen Type III - genetics</topic><topic>Collagen Type III - metabolism</topic><topic>Colonies</topic><topic>Colony-stimulating factor</topic><topic>Cytokines</topic><topic>Elastin</topic><topic>Elastin - genetics</topic><topic>Elastin - metabolism</topic><topic>Enzyme-linked immunosorbent assay</topic><topic>Experiments</topic><topic>Extracellular matrix</topic><topic>Extracellular Matrix - drug effects</topic><topic>Extracellular Matrix - genetics</topic><topic>Extracellular Matrix - metabolism</topic><topic>Fibroblasts</topic><topic>Fibroblasts - cytology</topic><topic>Fibroblasts - drug effects</topic><topic>Fibroblasts - metabolism</topic><topic>Fibronectin</topic><topic>Fibronectins</topic><topic>Fibronectins - antagonists & inhibitors</topic><topic>Fibronectins - genetics</topic><topic>Fibronectins - metabolism</topic><topic>Gene expression</topic><topic>Gene Expression - drug effects</topic><topic>Granulocyte-macrophage colony-stimulating factor</topic><topic>Granulocyte-Macrophage Colony-Stimulating Factor - metabolism</topic><topic>Granulocyte-Macrophage Colony-Stimulating Factor - pharmacology</topic><topic>Granulocytes</topic><topic>Growth factors</topic><topic>Health aspects</topic><topic>Hepatocyte Growth Factor - genetics</topic><topic>Hepatocyte Growth Factor - metabolism</topic><topic>Humans</topic><topic>Hyaluronic Acid - biosynthesis</topic><topic>Immunohistochemistry</topic><topic>In vitro methods and tests</topic><topic>In vivo methods and tests</topic><topic>Injections, Intralesional</topic><topic>Injuries</topic><topic>Interstitial collagenase</topic><topic>Macrophage colony stimulating factor</topic><topic>Macrophages</topic><topic>Matrix metalloproteinase</topic><topic>Medicine</topic><topic>mRNA</topic><topic>Mucosa</topic><topic>Otolaryngology</topic><topic>Polymerase chain reaction</topic><topic>Rabbits</topic><topic>Regeneration</topic><topic>RNA</topic><topic>Scars</topic><topic>Studies</topic><topic>Surgery</topic><topic>Synthesis</topic><topic>Tissue engineering</topic><topic>Transforming Growth Factor beta1 - 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Academic</collection><collection>ProQuest Engineering Collection</collection><collection>ProQuest Biological Science Collection</collection><collection>Agricultural Science Database</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biological Science Database</collection><collection>Engineering Database</collection><collection>Nursing & Allied Health Premium</collection><collection>Advanced Technologies & Aerospace Database</collection><collection>ProQuest Advanced Technologies & Aerospace Collection</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Environmental Science Database</collection><collection>Materials Science Collection</collection><collection>Publicly Available Content Database</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>Engineering Collection</collection><collection>Environmental Science Collection</collection><collection>Genetics Abstracts</collection><collection>PubMed Central (Full Participant titles)</collection><collection>DOAJ Directory of Open Access Journals</collection><jtitle>PloS one</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Lim, Jae-Yol</au><au>Choi, Byung Hyune</au><au>Lee, Songyi</au><au>Jang, Yun Ho</au><au>Choi, Jeong-Seok</au><au>Kim, Young-Mo</au><au>Zhang, Luo</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Regulation of wound healing by granulocyte-macrophage colony-stimulating factor after vocal fold injury</atitle><jtitle>PloS one</jtitle><addtitle>PLoS One</addtitle><date>2013-01-25</date><risdate>2013</risdate><volume>8</volume><issue>1</issue><spage>e54256</spage><pages>e54256-</pages><issn>1932-6203</issn><eissn>1932-6203</eissn><abstract><![CDATA[Vocal fold (VF) scarring remains a therapeutic challenge. Granulocyte-macrophage colony-stimulating factor (GM-CSF) facilitates epithelial wound healing, and recently, growth factor therapy has been applied to promote tissue repair. This study was undertaken to investigate the effect of GM-CSF on VF wound healing in vivo and in vitro.
VF scarring was induced in New Zealand white rabbits by direct injury. Immediately thereafter, either GM-CSF or PBS was injected into the VFs of rabbits. Endoscopic, histopathological, immunohistochemical, and biomechanical evaluations of VFs were performed at 3 months post-injury. Human vocal fold fibroblasts (hVFFs) were cultured with GM-CSF. Production of type I and III collagen was examined immunocytochemically, and the synthesis of elastin and hyaluronic acids was evaluated by ELISA. The mRNA levels of genes related to ECM components and ECM production-related growth factors, such as HGF and TGF-ß1, were examined by real time RT-PCR.
The GM-CSF-treated VFs showed reduced collagen deposition in comparison to the PBS-injected controls (P<0.05). Immunohistochemical staining revealed lower amounts of type I collagen and fibronectin in the GM-CSF-treated VFs (P<0.05 and P<0.01, respectively). Viscous and elastic shear moduli of VF samples were significantly lower in the GM-CSF group than in the PBS-injected group (P<0.001 and P<0.01, respectively). Mucosal waves in the GM-CSF group showed significant improvement when compared to the PBS group (P = 0.0446). GM-CSF inhibited TGF-β1-induced collagen synthesis by hVFFs (P<0.05) and the production of hyaluronic acids increased at 72 hours post-treatment (P<0.05). The expressions of HAS-2, tropoelastin, MMP-1, HGF, and c-Met mRNA were significantly increased by GM-CSF, although at different time points (P<0.05).
The present study shows that GM-CSF offers therapeutic potential for the remodeling of VF wounds and the promotion of VF regeneration.]]></abstract><cop>United States</cop><pub>Public Library of Science</pub><pmid>23372696</pmid><doi>10.1371/journal.pone.0054256</doi><tpages>e54256</tpages><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 1932-6203 |
| ispartof | PloS one, 2013-01, Vol.8 (1), p.e54256 |
| issn | 1932-6203 1932-6203 |
| language | eng |
| recordid | cdi_plos_journals_1327799852 |
| source | Public Library of Science (PLoS) Journals Open Access; MEDLINE; DOAJ Directory of Open Access Journals; EZB-FREE-00999 freely available EZB journals; PubMed Central; Free Full-Text Journals in Chemistry |
| subjects | Acids Animals Biology Biomechanics c-Met protein Cells, Cultured Collagen Collagen (type I) Collagen Type I - antagonists & inhibitors Collagen Type I - genetics Collagen Type I - metabolism Collagen Type III - genetics Collagen Type III - metabolism Colonies Colony-stimulating factor Cytokines Elastin Elastin - genetics Elastin - metabolism Enzyme-linked immunosorbent assay Experiments Extracellular matrix Extracellular Matrix - drug effects Extracellular Matrix - genetics Extracellular Matrix - metabolism Fibroblasts Fibroblasts - cytology Fibroblasts - drug effects Fibroblasts - metabolism Fibronectin Fibronectins Fibronectins - antagonists & inhibitors Fibronectins - genetics Fibronectins - metabolism Gene expression Gene Expression - drug effects Granulocyte-macrophage colony-stimulating factor Granulocyte-Macrophage Colony-Stimulating Factor - metabolism Granulocyte-Macrophage Colony-Stimulating Factor - pharmacology Granulocytes Growth factors Health aspects Hepatocyte Growth Factor - genetics Hepatocyte Growth Factor - metabolism Humans Hyaluronic Acid - biosynthesis Immunohistochemistry In vitro methods and tests In vivo methods and tests Injections, Intralesional Injuries Interstitial collagenase Macrophage colony stimulating factor Macrophages Matrix metalloproteinase Medicine mRNA Mucosa Otolaryngology Polymerase chain reaction Rabbits Regeneration RNA Scars Studies Surgery Synthesis Tissue engineering Transforming Growth Factor beta1 - genetics Transforming Growth Factor beta1 - metabolism Transforming growth factor-b1 Transforming growth factors Tropoelastin Vocal Cords - drug effects Vocal Cords - injuries Vocal Cords - metabolism Wound care Wound healing Wound Healing - drug effects Wound Healing - physiology |
| title | Regulation of wound healing by granulocyte-macrophage colony-stimulating factor after vocal fold injury |
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