Glycomic analysis of human respiratory tract tissues and correlation with influenza virus infection
The first step in influenza infection of the human respiratory tract is binding of the virus to sialic (Sia) acid terminated receptors. The binding of different strains of virus for the receptor is determined by the α linkage of the sialic acid to galactose and the adjacent glycan structure. In this...
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| creator | Walther, Trevenan Karamanska, Rositsa Chan, Renee W Y Chan, Michael C W Jia, Nan Air, Gillian Hopton, Clark Wong, Maria P Dell, Anne Malik Peiris, J S Haslam, Stuart M Nicholls, John M |
| description | The first step in influenza infection of the human respiratory tract is binding of the virus to sialic (Sia) acid terminated receptors. The binding of different strains of virus for the receptor is determined by the α linkage of the sialic acid to galactose and the adjacent glycan structure. In this study the N- and O-glycan composition of the human lung, bronchus and nasopharynx was characterized by mass spectrometry. Analysis showed that there was a wide spectrum of both Sia α2-3 and α2-6 glycans in the lung and bronchus. This glycan structural data was then utilized in combination with binding data from 4 of the published glycan arrays to assess whether these current glycan arrays were able to predict replication of human, avian and swine viruses in human ex vivo respiratory tract tissues. The most comprehensive array from the Consortium for Functional Glycomics contained the greatest diversity of sialylated glycans, but was not predictive of productive replication in the bronchus and lung. Our findings indicate that more comprehensive but focused arrays need to be developed to investigate influenza virus binding in an assessment of newly emerging influenza viruses. |
| doi_str_mv | 10.1371/journal.ppat.1003223 |
| format | Article |
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The binding of different strains of virus for the receptor is determined by the α linkage of the sialic acid to galactose and the adjacent glycan structure. In this study the N- and O-glycan composition of the human lung, bronchus and nasopharynx was characterized by mass spectrometry. Analysis showed that there was a wide spectrum of both Sia α2-3 and α2-6 glycans in the lung and bronchus. This glycan structural data was then utilized in combination with binding data from 4 of the published glycan arrays to assess whether these current glycan arrays were able to predict replication of human, avian and swine viruses in human ex vivo respiratory tract tissues. The most comprehensive array from the Consortium for Functional Glycomics contained the greatest diversity of sialylated glycans, but was not predictive of productive replication in the bronchus and lung. Our findings indicate that more comprehensive but focused arrays need to be developed to investigate influenza virus binding in an assessment of newly emerging influenza viruses.</description><identifier>ISSN: 1553-7374</identifier><identifier>ISSN: 1553-7366</identifier><identifier>EISSN: 1553-7374</identifier><identifier>DOI: 10.1371/journal.ppat.1003223</identifier><identifier>PMID: 23516363</identifier><language>eng</language><publisher>United States: Public Library of Science</publisher><subject>Adult ; Animals ; Arrays ; Avian flu ; Birds ; Bronchi - chemistry ; Bronchi - virology ; Cardiopulmonary system ; Cell Line ; Dogs ; Experiments ; Galactose - metabolism ; Glycomics ; Grants ; Health aspects ; Host-parasite relationships ; Humans ; Influenza A virus - physiology ; Influenza viruses ; Influenza, Human - metabolism ; Influenza, Human - virology ; Lung - chemistry ; Lung - virology ; Medicine ; Microarray Analysis ; Mortality ; N-Acetylneuraminic Acid - metabolism ; Pandemics ; Physiological aspects ; Polysaccharides - chemistry ; Polysaccharides - isolation & purification ; Receptors, Cell Surface - metabolism ; Respiratory organs ; Respiratory System - chemistry ; Respiratory System - virology ; Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization ; Studies ; Swine ; Viral Tropism ; Virus Attachment ; Virus Replication ; Viruses</subject><ispartof>PLoS pathogens, 2013-03, Vol.9 (3), p.e1003223-e1003223</ispartof><rights>COPYRIGHT 2013 Public Library of Science</rights><rights>2013 Walther et al 2013 Walther et al</rights><rights>2013 Walther et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited: Walther T, Karamanska R, Chan RWY, Chan MCW, Jia N, et al. (2013) Glycomic Analysis of Human Respiratory Tract Tissues and Correlation with Influenza Virus Infection. 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Karamanska, Rositsa ; Chan, Renee W Y ; Chan, Michael C W ; Jia, Nan ; Air, Gillian ; Hopton, Clark ; Wong, Maria P ; Dell, Anne ; Malik Peiris, J S ; Haslam, Stuart M ; Nicholls, John M</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c684t-dc3ed9d68e4a43c34bf21f512513476b36f998d886bbfad2de9774a3fffb518a3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2013</creationdate><topic>Adult</topic><topic>Animals</topic><topic>Arrays</topic><topic>Avian flu</topic><topic>Birds</topic><topic>Bronchi - chemistry</topic><topic>Bronchi - virology</topic><topic>Cardiopulmonary system</topic><topic>Cell Line</topic><topic>Dogs</topic><topic>Experiments</topic><topic>Galactose - metabolism</topic><topic>Glycomics</topic><topic>Grants</topic><topic>Health aspects</topic><topic>Host-parasite relationships</topic><topic>Humans</topic><topic>Influenza A virus - physiology</topic><topic>Influenza viruses</topic><topic>Influenza, Human - metabolism</topic><topic>Influenza, Human - virology</topic><topic>Lung - chemistry</topic><topic>Lung - virology</topic><topic>Medicine</topic><topic>Microarray Analysis</topic><topic>Mortality</topic><topic>N-Acetylneuraminic Acid - metabolism</topic><topic>Pandemics</topic><topic>Physiological aspects</topic><topic>Polysaccharides - chemistry</topic><topic>Polysaccharides - isolation & purification</topic><topic>Receptors, Cell Surface - metabolism</topic><topic>Respiratory organs</topic><topic>Respiratory System - chemistry</topic><topic>Respiratory System - virology</topic><topic>Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization</topic><topic>Studies</topic><topic>Swine</topic><topic>Viral Tropism</topic><topic>Virus Attachment</topic><topic>Virus Replication</topic><topic>Viruses</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Walther, Trevenan</creatorcontrib><creatorcontrib>Karamanska, Rositsa</creatorcontrib><creatorcontrib>Chan, Renee W Y</creatorcontrib><creatorcontrib>Chan, Michael C W</creatorcontrib><creatorcontrib>Jia, Nan</creatorcontrib><creatorcontrib>Air, Gillian</creatorcontrib><creatorcontrib>Hopton, Clark</creatorcontrib><creatorcontrib>Wong, Maria P</creatorcontrib><creatorcontrib>Dell, Anne</creatorcontrib><creatorcontrib>Malik Peiris, J S</creatorcontrib><creatorcontrib>Haslam, Stuart M</creatorcontrib><creatorcontrib>Nicholls, John M</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Gale In Context: Canada</collection><collection>Gale In Context: Science</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><collection>DOAJ Directory of Open Access Journals</collection><jtitle>PLoS pathogens</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Walther, Trevenan</au><au>Karamanska, Rositsa</au><au>Chan, Renee W Y</au><au>Chan, Michael C W</au><au>Jia, Nan</au><au>Air, Gillian</au><au>Hopton, Clark</au><au>Wong, Maria P</au><au>Dell, Anne</au><au>Malik Peiris, J S</au><au>Haslam, Stuart M</au><au>Nicholls, John M</au><au>Pekosz, Andrew</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Glycomic analysis of human respiratory tract tissues and correlation with influenza virus infection</atitle><jtitle>PLoS pathogens</jtitle><addtitle>PLoS Pathog</addtitle><date>2013-03-01</date><risdate>2013</risdate><volume>9</volume><issue>3</issue><spage>e1003223</spage><epage>e1003223</epage><pages>e1003223-e1003223</pages><issn>1553-7374</issn><issn>1553-7366</issn><eissn>1553-7374</eissn><abstract>The first step in influenza infection of the human respiratory tract is binding of the virus to sialic (Sia) acid terminated receptors. The binding of different strains of virus for the receptor is determined by the α linkage of the sialic acid to galactose and the adjacent glycan structure. In this study the N- and O-glycan composition of the human lung, bronchus and nasopharynx was characterized by mass spectrometry. Analysis showed that there was a wide spectrum of both Sia α2-3 and α2-6 glycans in the lung and bronchus. This glycan structural data was then utilized in combination with binding data from 4 of the published glycan arrays to assess whether these current glycan arrays were able to predict replication of human, avian and swine viruses in human ex vivo respiratory tract tissues. The most comprehensive array from the Consortium for Functional Glycomics contained the greatest diversity of sialylated glycans, but was not predictive of productive replication in the bronchus and lung. Our findings indicate that more comprehensive but focused arrays need to be developed to investigate influenza virus binding in an assessment of newly emerging influenza viruses.</abstract><cop>United States</cop><pub>Public Library of Science</pub><pmid>23516363</pmid><doi>10.1371/journal.ppat.1003223</doi><oa>free_for_read</oa></addata></record> |
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| subjects | Adult Animals Arrays Avian flu Birds Bronchi - chemistry Bronchi - virology Cardiopulmonary system Cell Line Dogs Experiments Galactose - metabolism Glycomics Grants Health aspects Host-parasite relationships Humans Influenza A virus - physiology Influenza viruses Influenza, Human - metabolism Influenza, Human - virology Lung - chemistry Lung - virology Medicine Microarray Analysis Mortality N-Acetylneuraminic Acid - metabolism Pandemics Physiological aspects Polysaccharides - chemistry Polysaccharides - isolation & purification Receptors, Cell Surface - metabolism Respiratory organs Respiratory System - chemistry Respiratory System - virology Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization Studies Swine Viral Tropism Virus Attachment Virus Replication Viruses |
| title | Glycomic analysis of human respiratory tract tissues and correlation with influenza virus infection |
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