Preparation and efficacy of a live newcastle disease virus vaccine encapsulated in chitosan nanoparticles
Newcastle disease (ND) is a highly contagious viral disease of poultry caused by pathogenic strains of the Newcastle disease virus (NDV). Live NDV vaccines are administered by drinking water, eyedrops or coarse aerosol spray. To further enhance mucosal immune responses, chitosan nanoparticles were d...
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| Veröffentlicht in: | PloS one 2012-12, Vol.7 (12), p.e53314 |
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| creator | Zhao, Kai Chen, Gang Shi, Xing-Ming Gao, Ting-Ting Li, Wei Zhao, Yan Zhang, Feng-Qiang Wu, Jin Cui, Xianlan Wang, Yun-Feng |
| description | Newcastle disease (ND) is a highly contagious viral disease of poultry caused by pathogenic strains of the Newcastle disease virus (NDV). Live NDV vaccines are administered by drinking water, eyedrops or coarse aerosol spray. To further enhance mucosal immune responses, chitosan nanoparticles were developed for the mucosal delivery of a live NDV vaccine.
A lentogenic live-virus vaccine (strain LaSota) against NDV encapsulated in chitosan nanoparticles were developed using an ionic crosslinking method. Chitosan nanoparticles containing the lentogenic live-virus vaccine against NDV (NDV-CS-NPs) were produced with good morphology, high stability, a mean diameter of 371.1 nm, an encapsulation rate of 77% and a zeta potential of +2.84 mV. The Western blotting analysis showed that NDV structural proteins were detected in NDV-CS-NPs. The virus release assay results of NDV-CS-NPs indicated that NDV was released from NDV-CS-NPs. Chickens immunized orally or intranasally with NDV-CS-NPs were fully protected whereas one out of five chickens immunized with the LaSota live NDV vaccine and three out of five chickens immunized with the inactivated NDV vaccine were dead after challenge with the highly virulent NDV strain F48E9.
NDV-CS-NPs induced better protection of immunized specific pathogen free chickens compared to the live NDV vaccine strain LaSota and the inactivated NDV vaccine. This study lays a foundation for the further development of mucosal vaccines and drugs encapsulated in chitosan nanoparticles. |
| doi_str_mv | 10.1371/journal.pone.0053314 |
| format | Article |
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A lentogenic live-virus vaccine (strain LaSota) against NDV encapsulated in chitosan nanoparticles were developed using an ionic crosslinking method. Chitosan nanoparticles containing the lentogenic live-virus vaccine against NDV (NDV-CS-NPs) were produced with good morphology, high stability, a mean diameter of 371.1 nm, an encapsulation rate of 77% and a zeta potential of +2.84 mV. The Western blotting analysis showed that NDV structural proteins were detected in NDV-CS-NPs. The virus release assay results of NDV-CS-NPs indicated that NDV was released from NDV-CS-NPs. Chickens immunized orally or intranasally with NDV-CS-NPs were fully protected whereas one out of five chickens immunized with the LaSota live NDV vaccine and three out of five chickens immunized with the inactivated NDV vaccine were dead after challenge with the highly virulent NDV strain F48E9.
NDV-CS-NPs induced better protection of immunized specific pathogen free chickens compared to the live NDV vaccine strain LaSota and the inactivated NDV vaccine. This study lays a foundation for the further development of mucosal vaccines and drugs encapsulated in chitosan nanoparticles.</description><identifier>ISSN: 1932-6203</identifier><identifier>EISSN: 1932-6203</identifier><identifier>DOI: 10.1371/journal.pone.0053314</identifier><identifier>PMID: 23285276</identifier><language>eng</language><publisher>United States: Public Library of Science</publisher><subject>Analysis ; Animal vaccines ; Animals ; Antigens ; Biology ; Biotechnology ; Cells, Cultured ; Chick Embryo ; Chickens ; Chickens - immunology ; Chitosan ; Chitosan - adverse effects ; Chitosan - chemistry ; Chitosan - pharmacology ; Crosslinking ; Deoxyribonucleic acid ; DNA ; Drinking water ; Drug Compounding - methods ; Drug delivery systems ; Drug development ; Drugs ; Encapsulation ; Engineering ; Ethics ; Immune response ; Immunization ; Immunosuppressive agents ; Infections ; Infectious diseases ; Laboratory animals ; Life sciences ; Materials Science ; Medicine ; Mucosal immunity ; Nanoparticles ; Nanoparticles - adverse effects ; Nanoparticles - chemistry ; Newcastle disease ; Newcastle Disease - immunology ; Newcastle Disease - prevention & control ; Newcastle disease virus - immunology ; Peptides ; Polymer crosslinking ; Polymers ; Poultry ; Poultry Diseases - immunology ; Poultry Diseases - prevention & control ; Proteins ; Random Allocation ; Specific pathogen free ; Structural proteins ; Treatment Outcome ; Vaccination - adverse effects ; Vaccination - methods ; Vaccination - veterinary ; Vaccines ; Vaccines, Attenuated - adverse effects ; Vaccines, Attenuated - biosynthesis ; Vaccines, Attenuated - chemistry ; Vaccines, Attenuated - therapeutic use ; Veterinary Science ; Viral Vaccines - adverse effects ; Viral Vaccines - biosynthesis ; Viral Vaccines - chemistry ; Viral Vaccines - therapeutic use ; Virulence (Microbiology) ; Viruses ; Western blotting ; Zeta potential</subject><ispartof>PloS one, 2012-12, Vol.7 (12), p.e53314</ispartof><rights>COPYRIGHT 2012 Public Library of Science</rights><rights>2012 Zhao et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License: https://creativecommons.org/licenses/by/4.0/ (the “License”), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><rights>2012 Zhao et al 2012 Zhao et al</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c692t-6635ee4d53b0fe6cc6368c1361aea1e3eeefa77e30758c4b2d44e5403ca711973</citedby><cites>FETCH-LOGICAL-c692t-6635ee4d53b0fe6cc6368c1361aea1e3eeefa77e30758c4b2d44e5403ca711973</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC3532065/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC3532065/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,723,776,780,860,881,2096,2915,23845,27901,27902,53766,53768,79569,79570</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/23285276$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Zhao, Kai</creatorcontrib><creatorcontrib>Chen, Gang</creatorcontrib><creatorcontrib>Shi, Xing-Ming</creatorcontrib><creatorcontrib>Gao, Ting-Ting</creatorcontrib><creatorcontrib>Li, Wei</creatorcontrib><creatorcontrib>Zhao, Yan</creatorcontrib><creatorcontrib>Zhang, Feng-Qiang</creatorcontrib><creatorcontrib>Wu, Jin</creatorcontrib><creatorcontrib>Cui, Xianlan</creatorcontrib><creatorcontrib>Wang, Yun-Feng</creatorcontrib><title>Preparation and efficacy of a live newcastle disease virus vaccine encapsulated in chitosan nanoparticles</title><title>PloS one</title><addtitle>PLoS One</addtitle><description>Newcastle disease (ND) is a highly contagious viral disease of poultry caused by pathogenic strains of the Newcastle disease virus (NDV). Live NDV vaccines are administered by drinking water, eyedrops or coarse aerosol spray. To further enhance mucosal immune responses, chitosan nanoparticles were developed for the mucosal delivery of a live NDV vaccine.
A lentogenic live-virus vaccine (strain LaSota) against NDV encapsulated in chitosan nanoparticles were developed using an ionic crosslinking method. Chitosan nanoparticles containing the lentogenic live-virus vaccine against NDV (NDV-CS-NPs) were produced with good morphology, high stability, a mean diameter of 371.1 nm, an encapsulation rate of 77% and a zeta potential of +2.84 mV. The Western blotting analysis showed that NDV structural proteins were detected in NDV-CS-NPs. The virus release assay results of NDV-CS-NPs indicated that NDV was released from NDV-CS-NPs. Chickens immunized orally or intranasally with NDV-CS-NPs were fully protected whereas one out of five chickens immunized with the LaSota live NDV vaccine and three out of five chickens immunized with the inactivated NDV vaccine were dead after challenge with the highly virulent NDV strain F48E9.
NDV-CS-NPs induced better protection of immunized specific pathogen free chickens compared to the live NDV vaccine strain LaSota and the inactivated NDV vaccine. 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adverse effects</subject><subject>Nanoparticles - chemistry</subject><subject>Newcastle disease</subject><subject>Newcastle Disease - immunology</subject><subject>Newcastle Disease - prevention & control</subject><subject>Newcastle disease virus - immunology</subject><subject>Peptides</subject><subject>Polymer crosslinking</subject><subject>Polymers</subject><subject>Poultry</subject><subject>Poultry Diseases - immunology</subject><subject>Poultry Diseases - prevention & control</subject><subject>Proteins</subject><subject>Random Allocation</subject><subject>Specific pathogen free</subject><subject>Structural proteins</subject><subject>Treatment Outcome</subject><subject>Vaccination - adverse effects</subject><subject>Vaccination - methods</subject><subject>Vaccination - veterinary</subject><subject>Vaccines</subject><subject>Vaccines, Attenuated - adverse effects</subject><subject>Vaccines, Attenuated - biosynthesis</subject><subject>Vaccines, Attenuated - chemistry</subject><subject>Vaccines, Attenuated - 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Live NDV vaccines are administered by drinking water, eyedrops or coarse aerosol spray. To further enhance mucosal immune responses, chitosan nanoparticles were developed for the mucosal delivery of a live NDV vaccine.
A lentogenic live-virus vaccine (strain LaSota) against NDV encapsulated in chitosan nanoparticles were developed using an ionic crosslinking method. Chitosan nanoparticles containing the lentogenic live-virus vaccine against NDV (NDV-CS-NPs) were produced with good morphology, high stability, a mean diameter of 371.1 nm, an encapsulation rate of 77% and a zeta potential of +2.84 mV. The Western blotting analysis showed that NDV structural proteins were detected in NDV-CS-NPs. The virus release assay results of NDV-CS-NPs indicated that NDV was released from NDV-CS-NPs. Chickens immunized orally or intranasally with NDV-CS-NPs were fully protected whereas one out of five chickens immunized with the LaSota live NDV vaccine and three out of five chickens immunized with the inactivated NDV vaccine were dead after challenge with the highly virulent NDV strain F48E9.
NDV-CS-NPs induced better protection of immunized specific pathogen free chickens compared to the live NDV vaccine strain LaSota and the inactivated NDV vaccine. This study lays a foundation for the further development of mucosal vaccines and drugs encapsulated in chitosan nanoparticles.</abstract><cop>United States</cop><pub>Public Library of Science</pub><pmid>23285276</pmid><doi>10.1371/journal.pone.0053314</doi><tpages>e53314</tpages><oa>free_for_read</oa></addata></record> |
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| recordid | cdi_plos_journals_1327232028 |
| source | MEDLINE; DOAJ Directory of Open Access Journals; EZB-FREE-00999 freely available EZB journals; PubMed Central; Free Full-Text Journals in Chemistry; Public Library of Science (PLoS) |
| subjects | Analysis Animal vaccines Animals Antigens Biology Biotechnology Cells, Cultured Chick Embryo Chickens Chickens - immunology Chitosan Chitosan - adverse effects Chitosan - chemistry Chitosan - pharmacology Crosslinking Deoxyribonucleic acid DNA Drinking water Drug Compounding - methods Drug delivery systems Drug development Drugs Encapsulation Engineering Ethics Immune response Immunization Immunosuppressive agents Infections Infectious diseases Laboratory animals Life sciences Materials Science Medicine Mucosal immunity Nanoparticles Nanoparticles - adverse effects Nanoparticles - chemistry Newcastle disease Newcastle Disease - immunology Newcastle Disease - prevention & control Newcastle disease virus - immunology Peptides Polymer crosslinking Polymers Poultry Poultry Diseases - immunology Poultry Diseases - prevention & control Proteins Random Allocation Specific pathogen free Structural proteins Treatment Outcome Vaccination - adverse effects Vaccination - methods Vaccination - veterinary Vaccines Vaccines, Attenuated - adverse effects Vaccines, Attenuated - biosynthesis Vaccines, Attenuated - chemistry Vaccines, Attenuated - therapeutic use Veterinary Science Viral Vaccines - adverse effects Viral Vaccines - biosynthesis Viral Vaccines - chemistry Viral Vaccines - therapeutic use Virulence (Microbiology) Viruses Western blotting Zeta potential |
| title | Preparation and efficacy of a live newcastle disease virus vaccine encapsulated in chitosan nanoparticles |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-02-15T17%3A09%3A11IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-gale_plos_&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Preparation%20and%20efficacy%20of%20a%20live%20newcastle%20disease%20virus%20vaccine%20encapsulated%20in%20chitosan%20nanoparticles&rft.jtitle=PloS%20one&rft.au=Zhao,%20Kai&rft.date=2012-12-28&rft.volume=7&rft.issue=12&rft.spage=e53314&rft.pages=e53314-&rft.issn=1932-6203&rft.eissn=1932-6203&rft_id=info:doi/10.1371/journal.pone.0053314&rft_dat=%3Cgale_plos_%3EA477013588%3C/gale_plos_%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=1327232028&rft_id=info:pmid/23285276&rft_galeid=A477013588&rft_doaj_id=oai_doaj_org_article_fdbab9beebd447f1978ee745ecfcdb7a&rfr_iscdi=true |