Advanced engineering of lipid metabolism in Nicotiana benthamiana using a draft genome and the V2 viral silencing-suppressor protein

The transient leaf assay in Nicotiana benthamiana is widely used in plant sciences, with one application being the rapid assembly of complex multigene pathways that produce new fatty acid profiles. This rapid and facile assay would be further improved if it were possible to simultaneously overexpres...

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Veröffentlicht in:	PloS one 2012-12, Vol.7 (12), p.e52717
Hauptverfasser:	Naim, Fatima, Nakasugi, Kenlee, Crowhurst, Ross N, Hilario, Elena, Zwart, Alexander B, Hellens, Roger P, Taylor, Jennifer M, Waterhouse, Peter M, Wood, Craig C
Format:	Artikel
Sprache:	eng
Schlagworte:	Agriculture Analysis Arabidopsis Assaying Begomovirus - genetics Biology Desaturation Elongation Engineering Enzymes Fatty Acid Desaturases - genetics Fatty Acid Desaturases - metabolism Fatty acids Gene expression Gene Expression Regulation, Plant Gene Knockdown Techniques Genes, Viral Genetic Engineering Genome, Plant Genomes Genomics High-Throughput Nucleotide Sequencing Industrial research Inverted Repeat Sequences Leaves Lipid metabolism Lipid Metabolism - genetics Lipids Metabolic engineering Metabolism Metabolites Nicotiana - enzymology Nicotiana - genetics Nicotiana benthamiana Physiology Plant diseases Plant Leaves - enzymology Plant Leaves - genetics Plant Oils - metabolism Plant Proteins - genetics Plant Proteins - metabolism Plants, Genetically Modified - enzymology Plants, Genetically Modified - genetics Polymerase chain reaction Proteins Ribonucleic acid RNA RNA, Small Interfering - genetics RNA-mediated interference Sequence Analysis, DNA siRNA Substrates Tomatoes Transgenes Viruses Yellow leaf
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