Comparative transcriptome profiling of dairy goat microRNAs from dry period and peak lactation mammary gland tissues
MicroRNAs (miRNAs) are small noncoding RNA molecules that serve as important post-transcriptional gene expression regulators by targeting messenger RNAs for post-transcriptional endonucleolytic cleavage or translational inhibition. miRNAs play important roles in many biological processes. Extensive...
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| creator | Li, Zhuanjian Lan, Xianyong Guo, Wenjiao Sun, Jiajie Huang, Yongzhen Wang, Jing Huang, Tinghua Lei, Chuozhao Fang, Xingtang Chen, Hong |
| description | MicroRNAs (miRNAs) are small noncoding RNA molecules that serve as important post-transcriptional gene expression regulators by targeting messenger RNAs for post-transcriptional endonucleolytic cleavage or translational inhibition. miRNAs play important roles in many biological processes. Extensive high-throughput sequencing studies of miRNAs have been performed in several animal models. However, little is known about the diversity of these regulatory RNAs in goat (Capra hircus), which is one of the most important agricultural animals and the oldest domesticated species raised worldwide. Goats have long been used for their milk, meat, hair (including cashmere), and skins throughout much of the world.
In this study, two small RNA libraries were constructed based on dry period and peak lactation dairy goat mammary gland tissues and sequenced using the Illumina-Solexa high-throughput sequencing technology. A total of 346 conserved and 95 novel miRNAs were identified in the dairy goat. miRNAs expression was confirmed by qRT-PCR in nine tissues and in the mammary gland during different stages of lactation. In addition, several candidate miRNAs that may be involved in mammary gland development and lactation were found by comparing the miRNA expression profiles in different tissues and developmental stages of the mammary gland.
This study reveals the first miRNAs profile related to the biology of the mammary gland in the dairy goat. The characterization of these miRNAs could contribute to a better understanding of the molecular mechanisms of lactation physiology and mammary gland development in the dairy goat. |
| doi_str_mv | 10.1371/journal.pone.0052388 |
| format | Article |
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In this study, two small RNA libraries were constructed based on dry period and peak lactation dairy goat mammary gland tissues and sequenced using the Illumina-Solexa high-throughput sequencing technology. A total of 346 conserved and 95 novel miRNAs were identified in the dairy goat. miRNAs expression was confirmed by qRT-PCR in nine tissues and in the mammary gland during different stages of lactation. In addition, several candidate miRNAs that may be involved in mammary gland development and lactation were found by comparing the miRNA expression profiles in different tissues and developmental stages of the mammary gland.
This study reveals the first miRNAs profile related to the biology of the mammary gland in the dairy goat. The characterization of these miRNAs could contribute to a better understanding of the molecular mechanisms of lactation physiology and mammary gland development in the dairy goat.</description><identifier>ISSN: 1932-6203</identifier><identifier>EISSN: 1932-6203</identifier><identifier>DOI: 10.1371/journal.pone.0052388</identifier><identifier>PMID: 23300659</identifier><language>eng</language><publisher>United States: Public Library of Science</publisher><subject>Agriculture ; Animal models ; Animal sciences ; Animals ; Antisense RNA ; Base Sequence ; Biological activity ; Biology ; Breast cancer ; Breasts ; Capra hircus ; Cashmere ; Cattle ; Comparative analysis ; Conserved Sequence ; Dairying ; Developmental stages ; Female ; Gene expression ; Gene Expression Profiling ; Gene Expression Regulation ; Genes ; Genetic aspects ; Goats ; Goats - genetics ; Goats - physiology ; High-Throughput Nucleotide Sequencing ; Immune system ; Immunology ; Inverted Repeat Sequences - genetics ; Laboratories ; Lactation ; Lactation - genetics ; Mammary gland ; Mammary Glands, Animal - metabolism ; Mammary Glands, Animal - physiology ; Meat ; MicroRNA ; MicroRNAs ; MicroRNAs - genetics ; Milk ; miRNA ; Molecular biology ; Molecular modelling ; Molecular Sequence Data ; Musculoskeletal system ; Next-generation sequencing ; Post-transcription ; Regulators ; Reproducibility of Results ; Ribonucleic acid ; RNA ; Rodents ; Sequence Analysis, RNA ; Sheep ; Skin ; Stem cells ; Studies ; Tissues ; Transcription (Genetics) ; Zoology</subject><ispartof>PloS one, 2012-12, Vol.7 (12), p.e52388-e52388</ispartof><rights>COPYRIGHT 2012 Public Library of Science</rights><rights>2012 Li et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License: https://creativecommons.org/licenses/by/4.0/ (the “License”), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><rights>2012 Li et al 2012 Li et al</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c758t-63db7e907db47be69dc3d6b4c70c6c749a10614e9c7eb0a68dc251982330e3d13</citedby><cites>FETCH-LOGICAL-c758t-63db7e907db47be69dc3d6b4c70c6c749a10614e9c7eb0a68dc251982330e3d13</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC3530564/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC3530564/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,723,776,780,860,881,2096,2915,23845,27901,27902,53766,53768,79569,79570</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/23300659$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Li, Zhuanjian</creatorcontrib><creatorcontrib>Lan, Xianyong</creatorcontrib><creatorcontrib>Guo, Wenjiao</creatorcontrib><creatorcontrib>Sun, Jiajie</creatorcontrib><creatorcontrib>Huang, Yongzhen</creatorcontrib><creatorcontrib>Wang, Jing</creatorcontrib><creatorcontrib>Huang, Tinghua</creatorcontrib><creatorcontrib>Lei, Chuozhao</creatorcontrib><creatorcontrib>Fang, Xingtang</creatorcontrib><creatorcontrib>Chen, Hong</creatorcontrib><title>Comparative transcriptome profiling of dairy goat microRNAs from dry period and peak lactation mammary gland tissues</title><title>PloS one</title><addtitle>PLoS One</addtitle><description>MicroRNAs (miRNAs) are small noncoding RNA molecules that serve as important post-transcriptional gene expression regulators by targeting messenger RNAs for post-transcriptional endonucleolytic cleavage or translational inhibition. miRNAs play important roles in many biological processes. Extensive high-throughput sequencing studies of miRNAs have been performed in several animal models. However, little is known about the diversity of these regulatory RNAs in goat (Capra hircus), which is one of the most important agricultural animals and the oldest domesticated species raised worldwide. Goats have long been used for their milk, meat, hair (including cashmere), and skins throughout much of the world.
In this study, two small RNA libraries were constructed based on dry period and peak lactation dairy goat mammary gland tissues and sequenced using the Illumina-Solexa high-throughput sequencing technology. A total of 346 conserved and 95 novel miRNAs were identified in the dairy goat. miRNAs expression was confirmed by qRT-PCR in nine tissues and in the mammary gland during different stages of lactation. In addition, several candidate miRNAs that may be involved in mammary gland development and lactation were found by comparing the miRNA expression profiles in different tissues and developmental stages of the mammary gland.
This study reveals the first miRNAs profile related to the biology of the mammary gland in the dairy goat. The characterization of these miRNAs could contribute to a better understanding of the molecular mechanisms of lactation physiology and mammary gland development in the dairy goat.</description><subject>Agriculture</subject><subject>Animal models</subject><subject>Animal sciences</subject><subject>Animals</subject><subject>Antisense RNA</subject><subject>Base Sequence</subject><subject>Biological activity</subject><subject>Biology</subject><subject>Breast cancer</subject><subject>Breasts</subject><subject>Capra hircus</subject><subject>Cashmere</subject><subject>Cattle</subject><subject>Comparative analysis</subject><subject>Conserved Sequence</subject><subject>Dairying</subject><subject>Developmental stages</subject><subject>Female</subject><subject>Gene expression</subject><subject>Gene Expression Profiling</subject><subject>Gene Expression Regulation</subject><subject>Genes</subject><subject>Genetic aspects</subject><subject>Goats</subject><subject>Goats - genetics</subject><subject>Goats - physiology</subject><subject>High-Throughput Nucleotide Sequencing</subject><subject>Immune system</subject><subject>Immunology</subject><subject>Inverted Repeat Sequences - genetics</subject><subject>Laboratories</subject><subject>Lactation</subject><subject>Lactation - genetics</subject><subject>Mammary gland</subject><subject>Mammary Glands, Animal - metabolism</subject><subject>Mammary Glands, Animal - physiology</subject><subject>Meat</subject><subject>MicroRNA</subject><subject>MicroRNAs</subject><subject>MicroRNAs - genetics</subject><subject>Milk</subject><subject>miRNA</subject><subject>Molecular biology</subject><subject>Molecular modelling</subject><subject>Molecular Sequence Data</subject><subject>Musculoskeletal system</subject><subject>Next-generation sequencing</subject><subject>Post-transcription</subject><subject>Regulators</subject><subject>Reproducibility of Results</subject><subject>Ribonucleic acid</subject><subject>RNA</subject><subject>Rodents</subject><subject>Sequence Analysis, RNA</subject><subject>Sheep</subject><subject>Skin</subject><subject>Stem cells</subject><subject>Studies</subject><subject>Tissues</subject><subject>Transcription 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transcriptome profiling of dairy goat microRNAs from dry period and peak lactation mammary gland tissues</title><author>Li, Zhuanjian ; Lan, Xianyong ; Guo, Wenjiao ; Sun, Jiajie ; Huang, Yongzhen ; Wang, Jing ; Huang, Tinghua ; Lei, Chuozhao ; Fang, Xingtang ; Chen, Hong</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c758t-63db7e907db47be69dc3d6b4c70c6c749a10614e9c7eb0a68dc251982330e3d13</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2012</creationdate><topic>Agriculture</topic><topic>Animal models</topic><topic>Animal sciences</topic><topic>Animals</topic><topic>Antisense RNA</topic><topic>Base Sequence</topic><topic>Biological activity</topic><topic>Biology</topic><topic>Breast cancer</topic><topic>Breasts</topic><topic>Capra hircus</topic><topic>Cashmere</topic><topic>Cattle</topic><topic>Comparative analysis</topic><topic>Conserved Sequence</topic><topic>Dairying</topic><topic>Developmental stages</topic><topic>Female</topic><topic>Gene expression</topic><topic>Gene Expression Profiling</topic><topic>Gene Expression Regulation</topic><topic>Genes</topic><topic>Genetic aspects</topic><topic>Goats</topic><topic>Goats - genetics</topic><topic>Goats - physiology</topic><topic>High-Throughput Nucleotide Sequencing</topic><topic>Immune system</topic><topic>Immunology</topic><topic>Inverted Repeat Sequences - genetics</topic><topic>Laboratories</topic><topic>Lactation</topic><topic>Lactation - genetics</topic><topic>Mammary gland</topic><topic>Mammary Glands, Animal - metabolism</topic><topic>Mammary Glands, Animal - physiology</topic><topic>Meat</topic><topic>MicroRNA</topic><topic>MicroRNAs</topic><topic>MicroRNAs - genetics</topic><topic>Milk</topic><topic>miRNA</topic><topic>Molecular biology</topic><topic>Molecular modelling</topic><topic>Molecular Sequence Data</topic><topic>Musculoskeletal 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Access Journals</collection><jtitle>PloS one</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Li, Zhuanjian</au><au>Lan, Xianyong</au><au>Guo, Wenjiao</au><au>Sun, Jiajie</au><au>Huang, Yongzhen</au><au>Wang, Jing</au><au>Huang, Tinghua</au><au>Lei, Chuozhao</au><au>Fang, Xingtang</au><au>Chen, Hong</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Comparative transcriptome profiling of dairy goat microRNAs from dry period and peak lactation mammary gland tissues</atitle><jtitle>PloS one</jtitle><addtitle>PLoS One</addtitle><date>2012-12-26</date><risdate>2012</risdate><volume>7</volume><issue>12</issue><spage>e52388</spage><epage>e52388</epage><pages>e52388-e52388</pages><issn>1932-6203</issn><eissn>1932-6203</eissn><abstract>MicroRNAs (miRNAs) are small noncoding RNA molecules that serve as important post-transcriptional gene expression regulators by targeting messenger RNAs for post-transcriptional endonucleolytic cleavage or translational inhibition. miRNAs play important roles in many biological processes. Extensive high-throughput sequencing studies of miRNAs have been performed in several animal models. However, little is known about the diversity of these regulatory RNAs in goat (Capra hircus), which is one of the most important agricultural animals and the oldest domesticated species raised worldwide. Goats have long been used for their milk, meat, hair (including cashmere), and skins throughout much of the world.
In this study, two small RNA libraries were constructed based on dry period and peak lactation dairy goat mammary gland tissues and sequenced using the Illumina-Solexa high-throughput sequencing technology. A total of 346 conserved and 95 novel miRNAs were identified in the dairy goat. miRNAs expression was confirmed by qRT-PCR in nine tissues and in the mammary gland during different stages of lactation. In addition, several candidate miRNAs that may be involved in mammary gland development and lactation were found by comparing the miRNA expression profiles in different tissues and developmental stages of the mammary gland.
This study reveals the first miRNAs profile related to the biology of the mammary gland in the dairy goat. The characterization of these miRNAs could contribute to a better understanding of the molecular mechanisms of lactation physiology and mammary gland development in the dairy goat.</abstract><cop>United States</cop><pub>Public Library of Science</pub><pmid>23300659</pmid><doi>10.1371/journal.pone.0052388</doi><tpages>e52388</tpages><oa>free_for_read</oa></addata></record> |
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| identifier | ISSN: 1932-6203 |
| ispartof | PloS one, 2012-12, Vol.7 (12), p.e52388-e52388 |
| issn | 1932-6203 1932-6203 |
| language | eng |
| recordid | cdi_plos_journals_1327218364 |
| source | MEDLINE; DOAJ Directory of Open Access Journals; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; PubMed Central; Free Full-Text Journals in Chemistry; Public Library of Science (PLoS) |
| subjects | Agriculture Animal models Animal sciences Animals Antisense RNA Base Sequence Biological activity Biology Breast cancer Breasts Capra hircus Cashmere Cattle Comparative analysis Conserved Sequence Dairying Developmental stages Female Gene expression Gene Expression Profiling Gene Expression Regulation Genes Genetic aspects Goats Goats - genetics Goats - physiology High-Throughput Nucleotide Sequencing Immune system Immunology Inverted Repeat Sequences - genetics Laboratories Lactation Lactation - genetics Mammary gland Mammary Glands, Animal - metabolism Mammary Glands, Animal - physiology Meat MicroRNA MicroRNAs MicroRNAs - genetics Milk miRNA Molecular biology Molecular modelling Molecular Sequence Data Musculoskeletal system Next-generation sequencing Post-transcription Regulators Reproducibility of Results Ribonucleic acid RNA Rodents Sequence Analysis, RNA Sheep Skin Stem cells Studies Tissues Transcription (Genetics) Zoology |
| title | Comparative transcriptome profiling of dairy goat microRNAs from dry period and peak lactation mammary gland tissues |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-02-15T10%3A23%3A19IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-gale_plos_&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Comparative%20transcriptome%20profiling%20of%20dairy%20goat%20microRNAs%20from%20dry%20period%20and%20peak%20lactation%20mammary%20gland%20tissues&rft.jtitle=PloS%20one&rft.au=Li,%20Zhuanjian&rft.date=2012-12-26&rft.volume=7&rft.issue=12&rft.spage=e52388&rft.epage=e52388&rft.pages=e52388-e52388&rft.issn=1932-6203&rft.eissn=1932-6203&rft_id=info:doi/10.1371/journal.pone.0052388&rft_dat=%3Cgale_plos_%3EA477041325%3C/gale_plos_%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=1327218364&rft_id=info:pmid/23300659&rft_galeid=A477041325&rft_doaj_id=oai_doaj_org_article_d367d7d243ed405c9d9cdb5f9ad50643&rfr_iscdi=true |