The fecal microbiome in dogs with acute diarrhea and idiopathic inflammatory bowel disease
Recent molecular studies have revealed a highly complex bacterial assembly in the canine intestinal tract. There is mounting evidence that microbes play an important role in the pathogenesis of acute and chronic enteropathies of dogs, including idiopathic inflammatory bowel disease (IBD). The aim of...
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| Veröffentlicht in: | PloS one 2012-12, Vol.7 (12), p.e51907-e51907 |
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| creator | Suchodolski, Jan S Markel, Melissa E Garcia-Mazcorro, Jose F Unterer, Stefan Heilmann, Romy M Dowd, Scot E Kachroo, Priyanka Ivanov, Ivan Minamoto, Yasushi Dillman, Enricka M Steiner, Jörg M Cook, Audrey K Toresson, Linda |
| description | Recent molecular studies have revealed a highly complex bacterial assembly in the canine intestinal tract. There is mounting evidence that microbes play an important role in the pathogenesis of acute and chronic enteropathies of dogs, including idiopathic inflammatory bowel disease (IBD). The aim of this study was to characterize the bacterial microbiota in dogs with various gastrointestinal disorders.
Fecal samples from healthy dogs (n = 32), dogs with acute non-hemorrhagic diarrhea (NHD; n = 12), dogs with acute hemorrhagic diarrhea (AHD; n = 13), and dogs with active (n = 9) and therapeutically controlled idiopathic IBD (n = 10) were analyzed by 454-pyrosequencing of the 16S rRNA gene and qPCR assays. Dogs with acute diarrhea, especially those with AHD, had the most profound alterations in their microbiome, as significant separations were observed on PCoA plots of unweighted Unifrac distances. Dogs with AHD had significant decreases in Blautia, Ruminococcaceae including Faecalibacterium, and Turicibacter spp., and significant increases in genus Sutterella and Clostridium perfringens when compared to healthy dogs. No significant separation on PCoA plots was observed for the dogs with IBD. Faecalibacterium spp. and Fusobacteria were, however, decreased in the dogs with clinically active IBD, but increased during time periods of clinically insignificant IBD, as defined by a clinical IBD activity index (CIBDAI).
Results of this study revealed a bacterial dysbiosis in fecal samples of dogs with various GI disorders. The observed changes in the microbiome differed between acute and chronic disease states. The bacterial groups that were commonly decreased during diarrhea are considered to be important short-chain fatty acid producers and may be important for canine intestinal health. Future studies should correlate these observed phylogenetic differences with functional changes in the intestinal microbiome of dogs with defined disease phenotypes. |
| doi_str_mv | 10.1371/journal.pone.0051907 |
| format | Article |
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Fecal samples from healthy dogs (n = 32), dogs with acute non-hemorrhagic diarrhea (NHD; n = 12), dogs with acute hemorrhagic diarrhea (AHD; n = 13), and dogs with active (n = 9) and therapeutically controlled idiopathic IBD (n = 10) were analyzed by 454-pyrosequencing of the 16S rRNA gene and qPCR assays. Dogs with acute diarrhea, especially those with AHD, had the most profound alterations in their microbiome, as significant separations were observed on PCoA plots of unweighted Unifrac distances. Dogs with AHD had significant decreases in Blautia, Ruminococcaceae including Faecalibacterium, and Turicibacter spp., and significant increases in genus Sutterella and Clostridium perfringens when compared to healthy dogs. No significant separation on PCoA plots was observed for the dogs with IBD. Faecalibacterium spp. and Fusobacteria were, however, decreased in the dogs with clinically active IBD, but increased during time periods of clinically insignificant IBD, as defined by a clinical IBD activity index (CIBDAI).
Results of this study revealed a bacterial dysbiosis in fecal samples of dogs with various GI disorders. The observed changes in the microbiome differed between acute and chronic disease states. The bacterial groups that were commonly decreased during diarrhea are considered to be important short-chain fatty acid producers and may be important for canine intestinal health. Future studies should correlate these observed phylogenetic differences with functional changes in the intestinal microbiome of dogs with defined disease phenotypes.</description><identifier>ISSN: 1932-6203</identifier><identifier>EISSN: 1932-6203</identifier><identifier>DOI: 10.1371/journal.pone.0051907</identifier><identifier>PMID: 23300577</identifier><language>eng</language><publisher>United States: Public Library of Science</publisher><subject>Active control ; Analysis ; Animals ; Bacteria ; Biology ; Biomarkers - metabolism ; Case-Control Studies ; Chronic diseases ; Chronic illnesses ; Clostridium perfringens ; Correlation analysis ; Diarrhea ; Diarrhea - genetics ; Diarrhea - microbiology ; Diarrhea - veterinary ; Disorders ; Dog Diseases - genetics ; Dog Diseases - microbiology ; Dogs ; Dysbacteriosis ; Faecalibacterium ; Fatty acids ; Fecal microflora ; Feces ; Feces - chemistry ; Feces - microbiology ; Female ; Gastrointestinal diseases ; Gene Expression Profiling ; Hemorrhage ; Inflammatory bowel disease ; Inflammatory bowel diseases ; Inflammatory Bowel Diseases - genetics ; Inflammatory Bowel Diseases - microbiology ; Inflammatory Bowel Diseases - veterinary ; Intestine ; Laboratories ; Male ; Medical research ; Medicine ; Metagenome - physiology ; Microbiota ; Microbiota (Symbiotic organisms) ; Oligonucleotide Array Sequence Analysis ; Pathogenesis ; Phylogenetics ; Phylogeny ; Real-Time Polymerase Chain Reaction ; Reverse Transcriptase Polymerase Chain Reaction ; RNA ; RNA, Messenger - genetics ; rRNA 16S ; Studies ; Veterinarians ; Veterinary colleges ; Veterinary medicine ; Veterinary Science</subject><ispartof>PloS one, 2012-12, Vol.7 (12), p.e51907-e51907</ispartof><rights>COPYRIGHT 2012 Public Library of Science</rights><rights>2012 Suchodolski et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License: https://creativecommons.org/licenses/by/4.0/ (the “License”), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><rights>2012 Suchodolski et al 2012 Suchodolski et al</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c692t-dd2d52e223c0fbc9b0ddeecfef2948fb621959bdc2c163e81abb970864cd78cd3</citedby><cites>FETCH-LOGICAL-c692t-dd2d52e223c0fbc9b0ddeecfef2948fb621959bdc2c163e81abb970864cd78cd3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC3530590/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC3530590/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,723,776,780,860,881,2096,2915,23845,27901,27902,53766,53768,79342,79343</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/23300577$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Suchodolski, Jan S</creatorcontrib><creatorcontrib>Markel, Melissa E</creatorcontrib><creatorcontrib>Garcia-Mazcorro, Jose F</creatorcontrib><creatorcontrib>Unterer, Stefan</creatorcontrib><creatorcontrib>Heilmann, Romy M</creatorcontrib><creatorcontrib>Dowd, Scot E</creatorcontrib><creatorcontrib>Kachroo, Priyanka</creatorcontrib><creatorcontrib>Ivanov, Ivan</creatorcontrib><creatorcontrib>Minamoto, Yasushi</creatorcontrib><creatorcontrib>Dillman, Enricka M</creatorcontrib><creatorcontrib>Steiner, Jörg M</creatorcontrib><creatorcontrib>Cook, Audrey K</creatorcontrib><creatorcontrib>Toresson, Linda</creatorcontrib><title>The fecal microbiome in dogs with acute diarrhea and idiopathic inflammatory bowel disease</title><title>PloS one</title><addtitle>PLoS One</addtitle><description>Recent molecular studies have revealed a highly complex bacterial assembly in the canine intestinal tract. There is mounting evidence that microbes play an important role in the pathogenesis of acute and chronic enteropathies of dogs, including idiopathic inflammatory bowel disease (IBD). The aim of this study was to characterize the bacterial microbiota in dogs with various gastrointestinal disorders.
Fecal samples from healthy dogs (n = 32), dogs with acute non-hemorrhagic diarrhea (NHD; n = 12), dogs with acute hemorrhagic diarrhea (AHD; n = 13), and dogs with active (n = 9) and therapeutically controlled idiopathic IBD (n = 10) were analyzed by 454-pyrosequencing of the 16S rRNA gene and qPCR assays. Dogs with acute diarrhea, especially those with AHD, had the most profound alterations in their microbiome, as significant separations were observed on PCoA plots of unweighted Unifrac distances. Dogs with AHD had significant decreases in Blautia, Ruminococcaceae including Faecalibacterium, and Turicibacter spp., and significant increases in genus Sutterella and Clostridium perfringens when compared to healthy dogs. No significant separation on PCoA plots was observed for the dogs with IBD. Faecalibacterium spp. and Fusobacteria were, however, decreased in the dogs with clinically active IBD, but increased during time periods of clinically insignificant IBD, as defined by a clinical IBD activity index (CIBDAI).
Results of this study revealed a bacterial dysbiosis in fecal samples of dogs with various GI disorders. The observed changes in the microbiome differed between acute and chronic disease states. The bacterial groups that were commonly decreased during diarrhea are considered to be important short-chain fatty acid producers and may be important for canine intestinal health. Future studies should correlate these observed phylogenetic differences with functional changes in the intestinal microbiome of dogs with defined disease phenotypes.</description><subject>Active control</subject><subject>Analysis</subject><subject>Animals</subject><subject>Bacteria</subject><subject>Biology</subject><subject>Biomarkers - metabolism</subject><subject>Case-Control Studies</subject><subject>Chronic diseases</subject><subject>Chronic illnesses</subject><subject>Clostridium perfringens</subject><subject>Correlation analysis</subject><subject>Diarrhea</subject><subject>Diarrhea - genetics</subject><subject>Diarrhea - microbiology</subject><subject>Diarrhea - veterinary</subject><subject>Disorders</subject><subject>Dog Diseases - genetics</subject><subject>Dog Diseases - microbiology</subject><subject>Dogs</subject><subject>Dysbacteriosis</subject><subject>Faecalibacterium</subject><subject>Fatty acids</subject><subject>Fecal microflora</subject><subject>Feces</subject><subject>Feces - 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Academic</collection><collection>PubMed Central (Full Participant titles)</collection><collection>DOAJ Directory of Open Access Journals</collection><jtitle>PloS one</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Suchodolski, Jan S</au><au>Markel, Melissa E</au><au>Garcia-Mazcorro, Jose F</au><au>Unterer, Stefan</au><au>Heilmann, Romy M</au><au>Dowd, Scot E</au><au>Kachroo, Priyanka</au><au>Ivanov, Ivan</au><au>Minamoto, Yasushi</au><au>Dillman, Enricka M</au><au>Steiner, Jörg M</au><au>Cook, Audrey K</au><au>Toresson, Linda</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>The fecal microbiome in dogs with acute diarrhea and idiopathic inflammatory bowel disease</atitle><jtitle>PloS one</jtitle><addtitle>PLoS One</addtitle><date>2012-12-26</date><risdate>2012</risdate><volume>7</volume><issue>12</issue><spage>e51907</spage><epage>e51907</epage><pages>e51907-e51907</pages><issn>1932-6203</issn><eissn>1932-6203</eissn><abstract>Recent molecular studies have revealed a highly complex bacterial assembly in the canine intestinal tract. There is mounting evidence that microbes play an important role in the pathogenesis of acute and chronic enteropathies of dogs, including idiopathic inflammatory bowel disease (IBD). The aim of this study was to characterize the bacterial microbiota in dogs with various gastrointestinal disorders.
Fecal samples from healthy dogs (n = 32), dogs with acute non-hemorrhagic diarrhea (NHD; n = 12), dogs with acute hemorrhagic diarrhea (AHD; n = 13), and dogs with active (n = 9) and therapeutically controlled idiopathic IBD (n = 10) were analyzed by 454-pyrosequencing of the 16S rRNA gene and qPCR assays. Dogs with acute diarrhea, especially those with AHD, had the most profound alterations in their microbiome, as significant separations were observed on PCoA plots of unweighted Unifrac distances. Dogs with AHD had significant decreases in Blautia, Ruminococcaceae including Faecalibacterium, and Turicibacter spp., and significant increases in genus Sutterella and Clostridium perfringens when compared to healthy dogs. No significant separation on PCoA plots was observed for the dogs with IBD. Faecalibacterium spp. and Fusobacteria were, however, decreased in the dogs with clinically active IBD, but increased during time periods of clinically insignificant IBD, as defined by a clinical IBD activity index (CIBDAI).
Results of this study revealed a bacterial dysbiosis in fecal samples of dogs with various GI disorders. The observed changes in the microbiome differed between acute and chronic disease states. The bacterial groups that were commonly decreased during diarrhea are considered to be important short-chain fatty acid producers and may be important for canine intestinal health. Future studies should correlate these observed phylogenetic differences with functional changes in the intestinal microbiome of dogs with defined disease phenotypes.</abstract><cop>United States</cop><pub>Public Library of Science</pub><pmid>23300577</pmid><doi>10.1371/journal.pone.0051907</doi><tpages>e51907</tpages><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 1932-6203 |
| ispartof | PloS one, 2012-12, Vol.7 (12), p.e51907-e51907 |
| issn | 1932-6203 1932-6203 |
| language | eng |
| recordid | cdi_plos_journals_1327215872 |
| source | MEDLINE; DOAJ Directory of Open Access Journals; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; PubMed Central; Free Full-Text Journals in Chemistry; Public Library of Science (PLoS) |
| subjects | Active control Analysis Animals Bacteria Biology Biomarkers - metabolism Case-Control Studies Chronic diseases Chronic illnesses Clostridium perfringens Correlation analysis Diarrhea Diarrhea - genetics Diarrhea - microbiology Diarrhea - veterinary Disorders Dog Diseases - genetics Dog Diseases - microbiology Dogs Dysbacteriosis Faecalibacterium Fatty acids Fecal microflora Feces Feces - chemistry Feces - microbiology Female Gastrointestinal diseases Gene Expression Profiling Hemorrhage Inflammatory bowel disease Inflammatory bowel diseases Inflammatory Bowel Diseases - genetics Inflammatory Bowel Diseases - microbiology Inflammatory Bowel Diseases - veterinary Intestine Laboratories Male Medical research Medicine Metagenome - physiology Microbiota Microbiota (Symbiotic organisms) Oligonucleotide Array Sequence Analysis Pathogenesis Phylogenetics Phylogeny Real-Time Polymerase Chain Reaction Reverse Transcriptase Polymerase Chain Reaction RNA RNA, Messenger - genetics rRNA 16S Studies Veterinarians Veterinary colleges Veterinary medicine Veterinary Science |
| title | The fecal microbiome in dogs with acute diarrhea and idiopathic inflammatory bowel disease |
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