Ultra-bright and -stable red and near-infrared squaraine fluorophores for in vivo two-photon imaging
Fluorescent dyes that are bright, stable, small, and biocompatible are needed for high-sensitivity two-photon imaging, but the combination of these traits has been elusive. We identified a class of squaraine derivatives with large two-photon action cross-sections (up to 10,000 GM) at near-infrared w...
Gespeichert in:
| Veröffentlicht in: | PloS one 2012-12, Vol.7 (12), p.e51980-e51980 |
|---|---|
| Hauptverfasser: | , , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | e51980 |
|---|---|
| container_issue | 12 |
| container_start_page | e51980 |
| container_title | PloS one |
| container_volume | 7 |
| creator | Podgorski, Kaspar Terpetschnig, Ewald Klochko, Oleksii P Obukhova, Olena M Haas, Kurt |
| description | Fluorescent dyes that are bright, stable, small, and biocompatible are needed for high-sensitivity two-photon imaging, but the combination of these traits has been elusive. We identified a class of squaraine derivatives with large two-photon action cross-sections (up to 10,000 GM) at near-infrared wavelengths critical for in vivo imaging. We demonstrate the biocompatibility and stability of a red-emitting squaraine-rotaxane (SeTau-647) by imaging dye-filled neurons in vivo over 5 days, and utility for sensitive subcellular imaging by synthesizing a specific peptide-conjugate label for the synaptic protein PSD-95. |
| doi_str_mv | 10.1371/journal.pone.0051980 |
| format | Article |
| fullrecord | <record><control><sourceid>gale_plos_</sourceid><recordid>TN_cdi_plos_journals_1327145932</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><galeid>A477083579</galeid><doaj_id>oai_doaj_org_article_b19845a80d584e9e8be81ebffcd898b2</doaj_id><sourcerecordid>A477083579</sourcerecordid><originalsourceid>FETCH-LOGICAL-c758t-c3c7fbbfd274481d71aa005e23bfb1e82d9f60df0971dbe5bc7254477cc45a273</originalsourceid><addsrcrecordid>eNqNk12L1DAUhoso7rr6D0QLguhFxyZpm_RGWBY_BhYW1PU25LOTIZPMJumo_97MTneZyl5IL9qcPufNOW9yiuIlqBcAYfBh7cfgmF1svVOLum5BT-pHxSnoEaw6WKPHR98nxbMY1xlCpOueFicQwRZ0uD4t5LVNgVU8mGGVSuZkWcXEuFVlUPJ27RQLlXE6sH0k3owsMONUqe3og9-ufFCx1D6UxpU7s_Nl-uWrHE7elWbDBuOG58UTzWxUL6b3WXH9-dOPi6_V5dWX5cX5ZSVwS1IlkMCacy0hbhoCJAaM5ZoVRFxzoAiUve5qqeseA8lVywWGbdNgLETTMojRWfH6oLu1PtLJoEgBghg0bTYjE8sDIT1b023IBYY_1DNDbwM-DJSFZIRVlGdDsyypZUsa1SvCFQGKay0k6Qnfa32cdhv5RkmhXHbSzkTnf5xZ0cHvKGoh7FCTBd5NAsHfjComujFRKGuZU37MdeeWEIBdAzL65h_04e4mamC5gXxmPu8r9qL0PNtUE9TiPlOLB6j8SLUxIt8mbXJ8lvB-lpCZpH6ngY0x0uX3b__PXv2cs2-P2JViNq2it2My3sU52BxAEXyMQel7k0FN98Nw5wbdDwOdhiGnvTo-oPuku9uP_gK-HwWJ</addsrcrecordid><sourcetype>Open Website</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>1327145932</pqid></control><display><type>article</type><title>Ultra-bright and -stable red and near-infrared squaraine fluorophores for in vivo two-photon imaging</title><source>MEDLINE</source><source>DOAJ Directory of Open Access Journals</source><source>Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals</source><source>PubMed Central</source><source>Free Full-Text Journals in Chemistry</source><source>Public Library of Science (PLoS)</source><creator>Podgorski, Kaspar ; Terpetschnig, Ewald ; Klochko, Oleksii P ; Obukhova, Olena M ; Haas, Kurt</creator><creatorcontrib>Podgorski, Kaspar ; Terpetschnig, Ewald ; Klochko, Oleksii P ; Obukhova, Olena M ; Haas, Kurt</creatorcontrib><description>Fluorescent dyes that are bright, stable, small, and biocompatible are needed for high-sensitivity two-photon imaging, but the combination of these traits has been elusive. We identified a class of squaraine derivatives with large two-photon action cross-sections (up to 10,000 GM) at near-infrared wavelengths critical for in vivo imaging. We demonstrate the biocompatibility and stability of a red-emitting squaraine-rotaxane (SeTau-647) by imaging dye-filled neurons in vivo over 5 days, and utility for sensitive subcellular imaging by synthesizing a specific peptide-conjugate label for the synaptic protein PSD-95.</description><identifier>ISSN: 1932-6203</identifier><identifier>EISSN: 1932-6203</identifier><identifier>DOI: 10.1371/journal.pone.0051980</identifier><identifier>PMID: 23251670</identifier><language>eng</language><publisher>United States: Public Library of Science</publisher><subject>Animals ; Biocompatibility ; Biology ; Brain research ; Chemical compounds ; Cyclobutanes - chemistry ; Cyclobutanes - metabolism ; Dyes ; Fluorescence ; Fluorescent dyes ; Fluorescent Dyes - chemistry ; Fluorescent Dyes - metabolism ; Fluorescent indicators ; Fluorophores ; I.R. radiation ; Imaging ; Light ; Materials Science ; Medicine ; Microscopy ; Microscopy, Fluorescence, Multiphoton - methods ; Nerve Tissue Proteins - chemistry ; Nerve Tissue Proteins - metabolism ; Neurons ; Neurons - chemistry ; Neurons - metabolism ; Peptides ; Phenols - chemistry ; Phenols - metabolism ; Photons ; Physiology ; Postsynaptic density proteins ; Quantum dots ; Rotaxanes - chemistry ; Rotaxanes - metabolism ; SAP90-PSD95 Associated Proteins ; Single crystals ; Synapses - chemistry ; Synapses - metabolism ; Wavelengths ; Xenopus laevis</subject><ispartof>PloS one, 2012-12, Vol.7 (12), p.e51980-e51980</ispartof><rights>COPYRIGHT 2012 Public Library of Science</rights><rights>2012 Podgorski et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License: https://creativecommons.org/licenses/by/4.0/ (the “License”), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><rights>2012 Podgorski et al 2012 Podgorski et al</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c758t-c3c7fbbfd274481d71aa005e23bfb1e82d9f60df0971dbe5bc7254477cc45a273</citedby><cites>FETCH-LOGICAL-c758t-c3c7fbbfd274481d71aa005e23bfb1e82d9f60df0971dbe5bc7254477cc45a273</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC3522634/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC3522634/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,723,776,780,860,881,2096,2915,23845,27901,27902,53766,53768,79342,79343</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/23251670$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Podgorski, Kaspar</creatorcontrib><creatorcontrib>Terpetschnig, Ewald</creatorcontrib><creatorcontrib>Klochko, Oleksii P</creatorcontrib><creatorcontrib>Obukhova, Olena M</creatorcontrib><creatorcontrib>Haas, Kurt</creatorcontrib><title>Ultra-bright and -stable red and near-infrared squaraine fluorophores for in vivo two-photon imaging</title><title>PloS one</title><addtitle>PLoS One</addtitle><description>Fluorescent dyes that are bright, stable, small, and biocompatible are needed for high-sensitivity two-photon imaging, but the combination of these traits has been elusive. We identified a class of squaraine derivatives with large two-photon action cross-sections (up to 10,000 GM) at near-infrared wavelengths critical for in vivo imaging. We demonstrate the biocompatibility and stability of a red-emitting squaraine-rotaxane (SeTau-647) by imaging dye-filled neurons in vivo over 5 days, and utility for sensitive subcellular imaging by synthesizing a specific peptide-conjugate label for the synaptic protein PSD-95.</description><subject>Animals</subject><subject>Biocompatibility</subject><subject>Biology</subject><subject>Brain research</subject><subject>Chemical compounds</subject><subject>Cyclobutanes - chemistry</subject><subject>Cyclobutanes - metabolism</subject><subject>Dyes</subject><subject>Fluorescence</subject><subject>Fluorescent dyes</subject><subject>Fluorescent Dyes - chemistry</subject><subject>Fluorescent Dyes - metabolism</subject><subject>Fluorescent indicators</subject><subject>Fluorophores</subject><subject>I.R. radiation</subject><subject>Imaging</subject><subject>Light</subject><subject>Materials Science</subject><subject>Medicine</subject><subject>Microscopy</subject><subject>Microscopy, Fluorescence, Multiphoton - methods</subject><subject>Nerve Tissue Proteins - chemistry</subject><subject>Nerve Tissue Proteins - metabolism</subject><subject>Neurons</subject><subject>Neurons - chemistry</subject><subject>Neurons - metabolism</subject><subject>Peptides</subject><subject>Phenols - chemistry</subject><subject>Phenols - metabolism</subject><subject>Photons</subject><subject>Physiology</subject><subject>Postsynaptic density proteins</subject><subject>Quantum dots</subject><subject>Rotaxanes - chemistry</subject><subject>Rotaxanes - metabolism</subject><subject>SAP90-PSD95 Associated Proteins</subject><subject>Single crystals</subject><subject>Synapses - chemistry</subject><subject>Synapses - metabolism</subject><subject>Wavelengths</subject><subject>Xenopus laevis</subject><issn>1932-6203</issn><issn>1932-6203</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2012</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>BENPR</sourceid><sourceid>DOA</sourceid><recordid>eNqNk12L1DAUhoso7rr6D0QLguhFxyZpm_RGWBY_BhYW1PU25LOTIZPMJumo_97MTneZyl5IL9qcPufNOW9yiuIlqBcAYfBh7cfgmF1svVOLum5BT-pHxSnoEaw6WKPHR98nxbMY1xlCpOueFicQwRZ0uD4t5LVNgVU8mGGVSuZkWcXEuFVlUPJ27RQLlXE6sH0k3owsMONUqe3og9-ufFCx1D6UxpU7s_Nl-uWrHE7elWbDBuOG58UTzWxUL6b3WXH9-dOPi6_V5dWX5cX5ZSVwS1IlkMCacy0hbhoCJAaM5ZoVRFxzoAiUve5qqeseA8lVywWGbdNgLETTMojRWfH6oLu1PtLJoEgBghg0bTYjE8sDIT1b023IBYY_1DNDbwM-DJSFZIRVlGdDsyypZUsa1SvCFQGKay0k6Qnfa32cdhv5RkmhXHbSzkTnf5xZ0cHvKGoh7FCTBd5NAsHfjComujFRKGuZU37MdeeWEIBdAzL65h_04e4mamC5gXxmPu8r9qL0PNtUE9TiPlOLB6j8SLUxIt8mbXJ8lvB-lpCZpH6ngY0x0uX3b__PXv2cs2-P2JViNq2it2My3sU52BxAEXyMQel7k0FN98Nw5wbdDwOdhiGnvTo-oPuku9uP_gK-HwWJ</recordid><startdate>20121214</startdate><enddate>20121214</enddate><creator>Podgorski, Kaspar</creator><creator>Terpetschnig, Ewald</creator><creator>Klochko, Oleksii P</creator><creator>Obukhova, Olena M</creator><creator>Haas, Kurt</creator><general>Public Library of Science</general><general>Public Library of Science (PLoS)</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>IOV</scope><scope>ISR</scope><scope>3V.</scope><scope>7QG</scope><scope>7QL</scope><scope>7QO</scope><scope>7RV</scope><scope>7SN</scope><scope>7SS</scope><scope>7T5</scope><scope>7TG</scope><scope>7TM</scope><scope>7U9</scope><scope>7X2</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>8AO</scope><scope>8C1</scope><scope>8FD</scope><scope>8FE</scope><scope>8FG</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABJCF</scope><scope>ABUWG</scope><scope>AEUYN</scope><scope>AFKRA</scope><scope>ARAPS</scope><scope>ATCPS</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BGLVJ</scope><scope>BHPHI</scope><scope>C1K</scope><scope>CCPQU</scope><scope>D1I</scope><scope>DWQXO</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>H94</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>KB.</scope><scope>KB0</scope><scope>KL.</scope><scope>L6V</scope><scope>LK8</scope><scope>M0K</scope><scope>M0S</scope><scope>M1P</scope><scope>M7N</scope><scope>M7P</scope><scope>M7S</scope><scope>NAPCQ</scope><scope>P5Z</scope><scope>P62</scope><scope>P64</scope><scope>PATMY</scope><scope>PDBOC</scope><scope>PIMPY</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>PTHSS</scope><scope>PYCSY</scope><scope>RC3</scope><scope>7X8</scope><scope>5PM</scope><scope>DOA</scope></search><sort><creationdate>20121214</creationdate><title>Ultra-bright and -stable red and near-infrared squaraine fluorophores for in vivo two-photon imaging</title><author>Podgorski, Kaspar ; Terpetschnig, Ewald ; Klochko, Oleksii P ; Obukhova, Olena M ; Haas, Kurt</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c758t-c3c7fbbfd274481d71aa005e23bfb1e82d9f60df0971dbe5bc7254477cc45a273</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2012</creationdate><topic>Animals</topic><topic>Biocompatibility</topic><topic>Biology</topic><topic>Brain research</topic><topic>Chemical compounds</topic><topic>Cyclobutanes - chemistry</topic><topic>Cyclobutanes - metabolism</topic><topic>Dyes</topic><topic>Fluorescence</topic><topic>Fluorescent dyes</topic><topic>Fluorescent Dyes - chemistry</topic><topic>Fluorescent Dyes - metabolism</topic><topic>Fluorescent indicators</topic><topic>Fluorophores</topic><topic>I.R. radiation</topic><topic>Imaging</topic><topic>Light</topic><topic>Materials Science</topic><topic>Medicine</topic><topic>Microscopy</topic><topic>Microscopy, Fluorescence, Multiphoton - methods</topic><topic>Nerve Tissue Proteins - chemistry</topic><topic>Nerve Tissue Proteins - metabolism</topic><topic>Neurons</topic><topic>Neurons - chemistry</topic><topic>Neurons - metabolism</topic><topic>Peptides</topic><topic>Phenols - chemistry</topic><topic>Phenols - metabolism</topic><topic>Photons</topic><topic>Physiology</topic><topic>Postsynaptic density proteins</topic><topic>Quantum dots</topic><topic>Rotaxanes - chemistry</topic><topic>Rotaxanes - metabolism</topic><topic>SAP90-PSD95 Associated Proteins</topic><topic>Single crystals</topic><topic>Synapses - chemistry</topic><topic>Synapses - metabolism</topic><topic>Wavelengths</topic><topic>Xenopus laevis</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Podgorski, Kaspar</creatorcontrib><creatorcontrib>Terpetschnig, Ewald</creatorcontrib><creatorcontrib>Klochko, Oleksii P</creatorcontrib><creatorcontrib>Obukhova, Olena M</creatorcontrib><creatorcontrib>Haas, Kurt</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Gale In Context: Opposing Viewpoints</collection><collection>Gale In Context: Science</collection><collection>ProQuest Central (Corporate)</collection><collection>Animal Behavior Abstracts</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Biotechnology Research Abstracts</collection><collection>Nursing & Allied Health Database</collection><collection>Ecology Abstracts</collection><collection>Entomology Abstracts (Full archive)</collection><collection>Immunology Abstracts</collection><collection>Meteorological & Geoastrophysical Abstracts</collection><collection>Nucleic Acids Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>Agricultural Science Collection</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Medical Database (Alumni Edition)</collection><collection>ProQuest Pharma Collection</collection><collection>Public Health Database</collection><collection>Technology Research Database</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Technology Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>Materials Science & Engineering Collection</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest One Sustainability</collection><collection>ProQuest Central UK/Ireland</collection><collection>Advanced Technologies & Aerospace Collection</collection><collection>Agricultural & Environmental Science Collection</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Technology Collection</collection><collection>Natural Science Collection</collection><collection>Environmental Sciences and Pollution Management</collection><collection>ProQuest One Community College</collection><collection>ProQuest Materials Science Collection</collection><collection>ProQuest Central Korea</collection><collection>Engineering Research Database</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Materials Science Database</collection><collection>Nursing & Allied Health Database (Alumni Edition)</collection><collection>Meteorological & Geoastrophysical Abstracts - Academic</collection><collection>ProQuest Engineering Collection</collection><collection>ProQuest Biological Science Collection</collection><collection>Agricultural Science Database</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biological Science Database</collection><collection>Engineering Database</collection><collection>Nursing & Allied Health Premium</collection><collection>Advanced Technologies & Aerospace Database</collection><collection>ProQuest Advanced Technologies & Aerospace Collection</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Environmental Science Database</collection><collection>Materials Science Collection</collection><collection>Publicly Available Content Database</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><collection>Engineering Collection</collection><collection>Environmental Science Collection</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><collection>DOAJ Directory of Open Access Journals</collection><jtitle>PloS one</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Podgorski, Kaspar</au><au>Terpetschnig, Ewald</au><au>Klochko, Oleksii P</au><au>Obukhova, Olena M</au><au>Haas, Kurt</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Ultra-bright and -stable red and near-infrared squaraine fluorophores for in vivo two-photon imaging</atitle><jtitle>PloS one</jtitle><addtitle>PLoS One</addtitle><date>2012-12-14</date><risdate>2012</risdate><volume>7</volume><issue>12</issue><spage>e51980</spage><epage>e51980</epage><pages>e51980-e51980</pages><issn>1932-6203</issn><eissn>1932-6203</eissn><abstract>Fluorescent dyes that are bright, stable, small, and biocompatible are needed for high-sensitivity two-photon imaging, but the combination of these traits has been elusive. We identified a class of squaraine derivatives with large two-photon action cross-sections (up to 10,000 GM) at near-infrared wavelengths critical for in vivo imaging. We demonstrate the biocompatibility and stability of a red-emitting squaraine-rotaxane (SeTau-647) by imaging dye-filled neurons in vivo over 5 days, and utility for sensitive subcellular imaging by synthesizing a specific peptide-conjugate label for the synaptic protein PSD-95.</abstract><cop>United States</cop><pub>Public Library of Science</pub><pmid>23251670</pmid><doi>10.1371/journal.pone.0051980</doi><tpages>e51980</tpages><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 1932-6203 |
| ispartof | PloS one, 2012-12, Vol.7 (12), p.e51980-e51980 |
| issn | 1932-6203 1932-6203 |
| language | eng |
| recordid | cdi_plos_journals_1327145932 |
| source | MEDLINE; DOAJ Directory of Open Access Journals; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; PubMed Central; Free Full-Text Journals in Chemistry; Public Library of Science (PLoS) |
| subjects | Animals Biocompatibility Biology Brain research Chemical compounds Cyclobutanes - chemistry Cyclobutanes - metabolism Dyes Fluorescence Fluorescent dyes Fluorescent Dyes - chemistry Fluorescent Dyes - metabolism Fluorescent indicators Fluorophores I.R. radiation Imaging Light Materials Science Medicine Microscopy Microscopy, Fluorescence, Multiphoton - methods Nerve Tissue Proteins - chemistry Nerve Tissue Proteins - metabolism Neurons Neurons - chemistry Neurons - metabolism Peptides Phenols - chemistry Phenols - metabolism Photons Physiology Postsynaptic density proteins Quantum dots Rotaxanes - chemistry Rotaxanes - metabolism SAP90-PSD95 Associated Proteins Single crystals Synapses - chemistry Synapses - metabolism Wavelengths Xenopus laevis |
| title | Ultra-bright and -stable red and near-infrared squaraine fluorophores for in vivo two-photon imaging |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-02-09T01%3A01%3A50IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-gale_plos_&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Ultra-bright%20and%20-stable%20red%20and%20near-infrared%20squaraine%20fluorophores%20for%20in%20vivo%20two-photon%20imaging&rft.jtitle=PloS%20one&rft.au=Podgorski,%20Kaspar&rft.date=2012-12-14&rft.volume=7&rft.issue=12&rft.spage=e51980&rft.epage=e51980&rft.pages=e51980-e51980&rft.issn=1932-6203&rft.eissn=1932-6203&rft_id=info:doi/10.1371/journal.pone.0051980&rft_dat=%3Cgale_plos_%3EA477083579%3C/gale_plos_%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=1327145932&rft_id=info:pmid/23251670&rft_galeid=A477083579&rft_doaj_id=oai_doaj_org_article_b19845a80d584e9e8be81ebffcd898b2&rfr_iscdi=true |