Porcine induced pluripotent stem cells require LIF and maintain their developmental potential in early stage of embryos
Porcine induced pluripotent stem (piPS) cell lines have been generated recently by using a cocktail of defined transcription factors, however, the features of authentic piPS cells have not been agreed upon and most of published iPS clones did not meet the stringent requirements of pluripotency. Here...
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| Veröffentlicht in: | PloS one 2012-12, Vol.7 (12), p.e51778 |
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| creator | Cheng, De Guo, Yanjie Li, Zhenzhen Liu, Yajun Gao, Xing Gao, Yi Cheng, Xiang Hu, Junhe Wang, Huayan |
| description | Porcine induced pluripotent stem (piPS) cell lines have been generated recently by using a cocktail of defined transcription factors, however, the features of authentic piPS cells have not been agreed upon and most of published iPS clones did not meet the stringent requirements of pluripotency. Here, we report the generation of piPS cells from fibroblasts using retrovirus carrying four mouse transcription factors (mOct4, mSox2, mKlf4 and mc-Myc, 4F). Multiple LIF-dependent piPS cell lines were generated and these cells showed the morphology similar to mouse embryonic stem cells and other pluripotent stem cells. In addition to the routine characterization, piPS cells were injected into porcine pre-compacted embryos to generate chimera embryos and nuclear transfer (NT) embryos. The results showed that piPS cells retain the ability to integrate into inner and outer layers of the blastocysts, and support the NT embryos development to blastocysts. The generations of chimera embryos and NT embryos derived from piPS clones are a practical means to determine the quality of iPS cells ex vivo. |
| doi_str_mv | 10.1371/journal.pone.0051778 |
| format | Article |
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Here, we report the generation of piPS cells from fibroblasts using retrovirus carrying four mouse transcription factors (mOct4, mSox2, mKlf4 and mc-Myc, 4F). Multiple LIF-dependent piPS cell lines were generated and these cells showed the morphology similar to mouse embryonic stem cells and other pluripotent stem cells. In addition to the routine characterization, piPS cells were injected into porcine pre-compacted embryos to generate chimera embryos and nuclear transfer (NT) embryos. The results showed that piPS cells retain the ability to integrate into inner and outer layers of the blastocysts, and support the NT embryos development to blastocysts. The generations of chimera embryos and NT embryos derived from piPS clones are a practical means to determine the quality of iPS cells ex vivo.</description><identifier>ISSN: 1932-6203</identifier><identifier>EISSN: 1932-6203</identifier><identifier>DOI: 10.1371/journal.pone.0051778</identifier><identifier>PMID: 23251622</identifier><language>eng</language><publisher>United States: Public Library of Science</publisher><subject>Animals ; Biology ; Biotechnology ; Blastocyst - cytology ; Blastocyst - drug effects ; Blastocysts ; Cell Differentiation - drug effects ; Cell lines ; Cell Proliferation - drug effects ; Cellular Reprogramming - drug effects ; Chimera ; Chimeras ; Cloning ; Cytology ; DNA binding proteins ; Embryo cells ; Embryo, Mammalian - cytology ; Embryo, Mammalian - drug effects ; Embryonic stem cells ; Embryos ; Fibroblasts ; Fibroblasts - cytology ; Fibroblasts - drug effects ; Fibroblasts - metabolism ; Genetic engineering ; Hogs ; In vitro fertilization ; Induced Pluripotent Stem Cells - cytology ; Induced Pluripotent Stem Cells - drug effects ; Induced Pluripotent Stem Cells - metabolism ; Leukemia Inhibitory Factor - pharmacology ; Mice ; Morphology ; Myc protein ; Nuclear transfer ; Nuclear Transfer Techniques ; Penicillin ; Pluripotency ; Retroviridae ; Stage-Specific Embryonic Antigens - metabolism ; Stem cell transplantation ; Stem cells ; Sus scrofa - embryology ; Transcription factors ; Transduction, Genetic ; Veterinary colleges ; Veterinary medicine</subject><ispartof>PloS one, 2012-12, Vol.7 (12), p.e51778</ispartof><rights>COPYRIGHT 2012 Public Library of Science</rights><rights>2012 Cheng et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License: https://creativecommons.org/licenses/by/4.0/ (the “License”), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. 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The generations of chimera embryos and NT embryos derived from piPS clones are a practical means to determine the quality of iPS cells ex vivo.</abstract><cop>United States</cop><pub>Public Library of Science</pub><pmid>23251622</pmid><doi>10.1371/journal.pone.0051778</doi><tpages>e51778</tpages><oa>free_for_read</oa></addata></record> |
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| subjects | Animals Biology Biotechnology Blastocyst - cytology Blastocyst - drug effects Blastocysts Cell Differentiation - drug effects Cell lines Cell Proliferation - drug effects Cellular Reprogramming - drug effects Chimera Chimeras Cloning Cytology DNA binding proteins Embryo cells Embryo, Mammalian - cytology Embryo, Mammalian - drug effects Embryonic stem cells Embryos Fibroblasts Fibroblasts - cytology Fibroblasts - drug effects Fibroblasts - metabolism Genetic engineering Hogs In vitro fertilization Induced Pluripotent Stem Cells - cytology Induced Pluripotent Stem Cells - drug effects Induced Pluripotent Stem Cells - metabolism Leukemia Inhibitory Factor - pharmacology Mice Morphology Myc protein Nuclear transfer Nuclear Transfer Techniques Penicillin Pluripotency Retroviridae Stage-Specific Embryonic Antigens - metabolism Stem cell transplantation Stem cells Sus scrofa - embryology Transcription factors Transduction, Genetic Veterinary colleges Veterinary medicine |
| title | Porcine induced pluripotent stem cells require LIF and maintain their developmental potential in early stage of embryos |
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