Stain-free quantification of chromosomes in live cells using regularized tomographic phase microscopy
Refractive index imaging is a label-free technique that enables long-term monitoring of the internal structures and molecular composition in living cells with minimal perturbation. Existing tomographic methods for the refractive index imaging lack 3-D resolution and result in artifacts that prevent...
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| Veröffentlicht in: | PloS one 2012-11, Vol.7 (11), p.e49502-e49502 |
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| creator | Sung, Yongjin Choi, Wonshik Lue, Niyom Dasari, Ramachandra R Yaqoob, Zahid |
| description | Refractive index imaging is a label-free technique that enables long-term monitoring of the internal structures and molecular composition in living cells with minimal perturbation. Existing tomographic methods for the refractive index imaging lack 3-D resolution and result in artifacts that prevent accurate refractive index quantification. To overcome these limitations without compromising the capability to observe a sample in its most native condition, we have developed a regularized tomographic phase microscope (RTPM) enabling accurate refractive index imaging of organelles inside intact cells. With the enhanced accuracy, we quantify the mass of chromosomes in intact living cells, and differentiate two human colon cancer lines, HT-29 and T84 cells, solely based on the non-aqueous (dry) mass of chromosomes. In addition, we demonstrate chromosomal imaging using a dual-wavelength RTPM, which shows its potential to determine the molecular composition of cellular organelles in live cells. |
| doi_str_mv | 10.1371/journal.pone.0049502 |
| format | Article |
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Existing tomographic methods for the refractive index imaging lack 3-D resolution and result in artifacts that prevent accurate refractive index quantification. To overcome these limitations without compromising the capability to observe a sample in its most native condition, we have developed a regularized tomographic phase microscope (RTPM) enabling accurate refractive index imaging of organelles inside intact cells. With the enhanced accuracy, we quantify the mass of chromosomes in intact living cells, and differentiate two human colon cancer lines, HT-29 and T84 cells, solely based on the non-aqueous (dry) mass of chromosomes. In addition, we demonstrate chromosomal imaging using a dual-wavelength RTPM, which shows its potential to determine the molecular composition of cellular organelles in live cells.</description><identifier>ISSN: 1932-6203</identifier><identifier>EISSN: 1932-6203</identifier><identifier>DOI: 10.1371/journal.pone.0049502</identifier><identifier>PMID: 23166689</identifier><language>eng</language><publisher>United States: Public Library of Science</publisher><subject>Biology ; Cell Line, Tumor ; Cells (biology) ; Chromosomes ; Colon ; Colon cancer ; Colorectal cancer ; Engineering ; Fourier transforms ; Humans ; Image Processing, Computer-Assisted ; Imaging ; Imaging, Three-Dimensional ; Information theory ; Interferometry ; Inverse problems ; Laboratories ; Light ; Medical imaging ; Medicine ; Microscopy ; Microscopy, Phase-Contrast - methods ; Optics ; Organelles ; Physics ; Refractive index ; Refractivity ; Refractometry ; Reproducibility of Results ; Spectrum analysis ; Stains ; Tomography, Optical - methods</subject><ispartof>PloS one, 2012-11, Vol.7 (11), p.e49502-e49502</ispartof><rights>COPYRIGHT 2012 Public Library of Science</rights><rights>2012 Sung et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License: https://creativecommons.org/licenses/by/4.0/ (the “License”), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><rights>2012 Sung et al 2012 Sung et al</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c692t-e4b2d8bde05f9a7ad7a5bcfeee122f2779e3e64d3affc1629ab8895bf069c873</citedby><cites>FETCH-LOGICAL-c692t-e4b2d8bde05f9a7ad7a5bcfeee122f2779e3e64d3affc1629ab8895bf069c873</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC3500303/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC3500303/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,723,776,780,860,881,2096,2915,23845,27901,27902,53766,53768,79343,79344</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/23166689$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><contributor>Coles, Jonathan A.</contributor><creatorcontrib>Sung, Yongjin</creatorcontrib><creatorcontrib>Choi, Wonshik</creatorcontrib><creatorcontrib>Lue, Niyom</creatorcontrib><creatorcontrib>Dasari, Ramachandra R</creatorcontrib><creatorcontrib>Yaqoob, Zahid</creatorcontrib><title>Stain-free quantification of chromosomes in live cells using regularized tomographic phase microscopy</title><title>PloS one</title><addtitle>PLoS One</addtitle><description>Refractive index imaging is a label-free technique that enables long-term monitoring of the internal structures and molecular composition in living cells with minimal perturbation. Existing tomographic methods for the refractive index imaging lack 3-D resolution and result in artifacts that prevent accurate refractive index quantification. To overcome these limitations without compromising the capability to observe a sample in its most native condition, we have developed a regularized tomographic phase microscope (RTPM) enabling accurate refractive index imaging of organelles inside intact cells. With the enhanced accuracy, we quantify the mass of chromosomes in intact living cells, and differentiate two human colon cancer lines, HT-29 and T84 cells, solely based on the non-aqueous (dry) mass of chromosomes. 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Existing tomographic methods for the refractive index imaging lack 3-D resolution and result in artifacts that prevent accurate refractive index quantification. To overcome these limitations without compromising the capability to observe a sample in its most native condition, we have developed a regularized tomographic phase microscope (RTPM) enabling accurate refractive index imaging of organelles inside intact cells. With the enhanced accuracy, we quantify the mass of chromosomes in intact living cells, and differentiate two human colon cancer lines, HT-29 and T84 cells, solely based on the non-aqueous (dry) mass of chromosomes. 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| subjects | Biology Cell Line, Tumor Cells (biology) Chromosomes Colon Colon cancer Colorectal cancer Engineering Fourier transforms Humans Image Processing, Computer-Assisted Imaging Imaging, Three-Dimensional Information theory Interferometry Inverse problems Laboratories Light Medical imaging Medicine Microscopy Microscopy, Phase-Contrast - methods Optics Organelles Physics Refractive index Refractivity Refractometry Reproducibility of Results Spectrum analysis Stains Tomography, Optical - methods |
| title | Stain-free quantification of chromosomes in live cells using regularized tomographic phase microscopy |
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