Proteomic analysis of the increased stress tolerance of saccharomyces cerevisiae encapsulated in liquid core alginate-chitosan capsules
Saccharomyces cerevisiae CBS8066 encapsulated in semi-permeable alginate or alginate-chitosan liquid core capsules have been shown to have an enhanced tolerance towards complex dilute-acid lignocellulose hydrolysates and the lignocellulose-derived inhibitor furfural, as well as towards high temperat...
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| Veröffentlicht in: | PloS one 2012-11, Vol.7 (11), p.e49335 |
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| description | Saccharomyces cerevisiae CBS8066 encapsulated in semi-permeable alginate or alginate-chitosan liquid core capsules have been shown to have an enhanced tolerance towards complex dilute-acid lignocellulose hydrolysates and the lignocellulose-derived inhibitor furfural, as well as towards high temperatures. The underlying molecular reasons for these effects have however not been elucidated. In this study we have investigated the response of the encapsulation on the proteome level in the yeast cells, in comparison with cells grown freely in suspension under otherwise similar conditions. The proteomic analysis was performed on whole cell protein extracts using nLC-MS/MS with TMT® labelling and 2-D DIGE. 842 and 52 proteins were identified using each method, respectively. The abundances of 213 proteins were significantly different between encapsulated and suspended cells, with good correlation between the fold change ratios obtained by the two methods for proteins identified in both. Encapsulation of the yeast caused an up-regulation of glucose-repressed proteins and of both general and starvation-specific stress responses, such as the trehalose biosynthesis pathway, and down-regulation of proteins linked to growth and protein synthesis. The encapsulation leads to a lack of nutrients for cells close to the core of the capsule due to mass transfer limitations. The triggering of the stress response may be beneficial for the cells in certain conditions, for example leading to the increased tolerance towards high temperatures and certain inhibitors. |
| doi_str_mv | 10.1371/journal.pone.0049335 |
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The underlying molecular reasons for these effects have however not been elucidated. In this study we have investigated the response of the encapsulation on the proteome level in the yeast cells, in comparison with cells grown freely in suspension under otherwise similar conditions. The proteomic analysis was performed on whole cell protein extracts using nLC-MS/MS with TMT® labelling and 2-D DIGE. 842 and 52 proteins were identified using each method, respectively. The abundances of 213 proteins were significantly different between encapsulated and suspended cells, with good correlation between the fold change ratios obtained by the two methods for proteins identified in both. Encapsulation of the yeast caused an up-regulation of glucose-repressed proteins and of both general and starvation-specific stress responses, such as the trehalose biosynthesis pathway, and down-regulation of proteins linked to growth and protein synthesis. 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The underlying molecular reasons for these effects have however not been elucidated. In this study we have investigated the response of the encapsulation on the proteome level in the yeast cells, in comparison with cells grown freely in suspension under otherwise similar conditions. The proteomic analysis was performed on whole cell protein extracts using nLC-MS/MS with TMT® labelling and 2-D DIGE. 842 and 52 proteins were identified using each method, respectively. The abundances of 213 proteins were significantly different between encapsulated and suspended cells, with good correlation between the fold change ratios obtained by the two methods for proteins identified in both. Encapsulation of the yeast caused an up-regulation of glucose-repressed proteins and of both general and starvation-specific stress responses, such as the trehalose biosynthesis pathway, and down-regulation of proteins linked to growth and protein synthesis. The encapsulation leads to a lack of nutrients for cells close to the core of the capsule due to mass transfer limitations. The triggering of the stress response may be beneficial for the cells in certain conditions, for example leading to the increased tolerance towards high temperatures and certain inhibitors.</abstract><cop>United States</cop><pub>Public Library of Science</pub><pmid>23152898</pmid><doi>10.1371/journal.pone.0049335</doi><tpages>e49335</tpages><oa>free_for_read</oa></addata></record> |
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| recordid | cdi_plos_journals_1326739755 |
| source | MEDLINE; DOAJ Directory of Open Access Journals; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; PubMed Central; SWEPUB Freely available online; Free Full-Text Journals in Chemistry; Public Library of Science (PLoS) |
| subjects | Adaptation, Physiological - drug effects Alginates - pharmacology Alginic acid Anaerobiosis - drug effects Analysis Baking yeast Batch Cell Culture Techniques Biology Biosynthesis Capsules Carbon - metabolism Cells, Immobilized - drug effects Cells, Immobilized - metabolism Chitosan Chitosan - pharmacology Chromatography, Liquid Dilution Down-Regulation - drug effects Electrophoresis, Gel, Two-Dimensional Encapsulation Engineering Engineering schools Enzymes Fermentation Fermentation - drug effects Furaldehyde - pharmacology Furfural Gene expression Genomes Glucose Glucuronic Acid - pharmacology Hexuronic Acids - pharmacology High temperature Hydrolysates Identification methods Labeling Labelling Lignocellulose Mass Spectrometry Mass transfer Nutrients Physiological aspects Physiology Protein biosynthesis Protein Biosynthesis - drug effects Protein synthesis Proteins Proteome - metabolism Proteomics Proteomics - methods Resource Recovery Resursåtervinning Saccharomyces cerevisiae Saccharomyces cerevisiae - cytology Saccharomyces cerevisiae - drug effects Saccharomyces cerevisiae - physiology Saccharomyces cerevisiae Proteins - metabolism Stress Stress, Physiological - drug effects Stresses Trehalose Up-Regulation - drug effects Yeast Yeasts |
| title | Proteomic analysis of the increased stress tolerance of saccharomyces cerevisiae encapsulated in liquid core alginate-chitosan capsules |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-02-12T17%3A18%3A35IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-gale_plos_&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Proteomic%20analysis%20of%20the%20increased%20stress%20tolerance%20of%20saccharomyces%20cerevisiae%20encapsulated%20in%20liquid%20core%20alginate-chitosan%20capsules&rft.jtitle=PloS%20one&rft.au=Westman,%20Johan%20O&rft.date=2012-11-09&rft.volume=7&rft.issue=11&rft.spage=e49335&rft.pages=e49335-&rft.issn=1932-6203&rft.eissn=1932-6203&rft_id=info:doi/10.1371/journal.pone.0049335&rft_dat=%3Cgale_plos_%3EA477091524%3C/gale_plos_%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=1326739755&rft_id=info:pmid/23152898&rft_galeid=A477091524&rft_doaj_id=oai_doaj_org_article_412a907f087d40adb66a3e6af92470ba&rfr_iscdi=true |