Detection of bacterial 16S rRNA and identification of four clinically important bacteria by real-time PCR

Within the paradigm of clinical infectious disease research, Acinetobacter baumannii, Escherichia coli, Klebsiella pneumoniae, and Pseudomonas aeruginosa represent the four most clinically relevant, and hence most extensively studied bacteria. Current culture-based methods for identifying these orga...

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Veröffentlicht in:	PloS one 2012-11, Vol.7 (11), p.e48558-e48558
Hauptverfasser:	Clifford, Robert J, Milillo, Michael, Prestwood, Jackson, Quintero, Reyes, Zurawski, Daniel V, Kwak, Yoon I, Waterman, Paige E, Lesho, Emil P, Mc Gann, Patrick
Format:	Artikel
Sprache:	eng
Schlagworte:	Bacteria Bacteria - classification Bacteria - genetics Bacteria - isolation & purification Biology Clinical isolates Communicable diseases Design DNA Primers - metabolism E coli Escherichia coli Gene sequencing Genes Genomes Genomics Gram-negative bacteria Homology Humans Identification methods Infectious diseases Klebsiella Klebsiella pneumoniae Laboratories Medicine Nucleic Acid Denaturation - genetics Organisms Pathogens Pneumonia Primers Proteins Pseudomonas aeruginosa Real time Real-Time Polymerase Chain Reaction - methods Ribosomal DNA RNA RNA, Bacterial - analysis RNA, Bacterial - genetics RNA, Ribosomal, 16S - analysis RNA, Ribosomal, 16S - genetics rRNA 16S Species Species Specificity Staphylococcus infections Surveillance UidA gene
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