A complex set of sex pheromones identified in the cuttlefish Sepia officinalis
The cephalopod mollusk Sepia officinalis can be considered as a relevant model for studying reproduction strategies associated to seasonal migrations. Using transcriptomic and peptidomic approaches, we aim to identify peptide sex pheromones that are thought to induce the aggregation of mature cuttle...
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| Veröffentlicht in: | PloS one 2012-10, Vol.7 (10), p.e46531-e46531 |
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| creator | Enault, Jérémy Zatylny-Gaudin, Céline Bernay, Benoît Lefranc, Benjamin Leprince, Jérôme Baudy-Floc'h, Michèle Henry, Joël |
| description | The cephalopod mollusk Sepia officinalis can be considered as a relevant model for studying reproduction strategies associated to seasonal migrations. Using transcriptomic and peptidomic approaches, we aim to identify peptide sex pheromones that are thought to induce the aggregation of mature cuttlefish in their egg-laying areas.
To facilitate the identification of sex pheromones, 576 5'-expressed sequence tags (ESTs) were sequenced from a single cDNA library generated from accessory sex glands of female cuttlefish. Our analysis yielded 223 unique sequences composed of 186 singletons and 37 contigs. Three major redundant ESTs called SPα, SPα' and SPβ were identified as good candidates for putative sex pheromone transcripts and are part of the 87 unique sequences classified as unknown. The alignment of translated SPα and SPα' revealed a high level of conservation, with 98.4% identity. Translation led to a 248-amino acid precursor containing six peptides with multiple putative disulfide bonds. The alignment of SPα-α' with SPβ revealed a partial structural conservation, with 37.3% identity. Translation of SPβ led to a 252-amino acid precursor containing five peptides. The occurrence of a signal peptide on SPα, SPα' and SPβ showed that the peptides were secreted. RT-PCR and mass spectrometry analyses revealed a co-localization of transcripts and expression products in the oviduct gland. Preliminary in vitro experiments performed on gills and penises revealed target organs involved in mating and ventilation.
The analysis of the accessory sex gland transcriptome of Sepia officinalis led to the identification of peptidic sex pheromones. Although preliminary functional tests suggested the involvement of the α3 and β2 peptides in ventilation and mating stimulation, further functional investigations will make it possible to identify the complete set of biological activities expected from waterborne pheromones. |
| doi_str_mv | 10.1371/journal.pone.0046531 |
| format | Article |
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To facilitate the identification of sex pheromones, 576 5'-expressed sequence tags (ESTs) were sequenced from a single cDNA library generated from accessory sex glands of female cuttlefish. Our analysis yielded 223 unique sequences composed of 186 singletons and 37 contigs. Three major redundant ESTs called SPα, SPα' and SPβ were identified as good candidates for putative sex pheromone transcripts and are part of the 87 unique sequences classified as unknown. The alignment of translated SPα and SPα' revealed a high level of conservation, with 98.4% identity. Translation led to a 248-amino acid precursor containing six peptides with multiple putative disulfide bonds. The alignment of SPα-α' with SPβ revealed a partial structural conservation, with 37.3% identity. Translation of SPβ led to a 252-amino acid precursor containing five peptides. The occurrence of a signal peptide on SPα, SPα' and SPβ showed that the peptides were secreted. RT-PCR and mass spectrometry analyses revealed a co-localization of transcripts and expression products in the oviduct gland. Preliminary in vitro experiments performed on gills and penises revealed target organs involved in mating and ventilation.
The analysis of the accessory sex gland transcriptome of Sepia officinalis led to the identification of peptidic sex pheromones. Although preliminary functional tests suggested the involvement of the α3 and β2 peptides in ventilation and mating stimulation, further functional investigations will make it possible to identify the complete set of biological activities expected from waterborne pheromones.</description><identifier>ISSN: 1932-6203</identifier><identifier>EISSN: 1932-6203</identifier><identifier>DOI: 10.1371/journal.pone.0046531</identifier><identifier>PMID: 23118854</identifier><language>eng</language><publisher>United States: Public Library of Science</publisher><subject>Acids ; Alignment ; Amino Acid Sequence ; Amino acids ; Animal behavior ; Animal Migration - physiology ; Animals ; Aplysia ; Bioinformatics ; Biology ; Chemical bonds ; Chemical Sciences ; Communication ; Conservation ; Decapodiformes - genetics ; Decapodiformes - metabolism ; Decapodiformes - physiology ; Disulfide bonds ; Egg laying ; Eggs ; Expressed sequence tags ; Expressed Sequence Tags - metabolism ; Female ; Gene expression ; Gene Expression Profiling ; Gene Library ; Genomic libraries ; Genomics ; Gills ; Glands ; Localization ; Mass Spectrometry ; Mass spectroscopy ; Mating ; Molecular Sequence Data ; Ontology ; Organic chemistry ; Organs ; Oviduct ; Peptides ; Peptides - genetics ; Peptides - metabolism ; Pheromones ; Polymerase chain reaction ; Proteins ; Reproduction - physiology ; Seasons ; Sepia officinalis ; Sequence Analysis, DNA ; Sex ; Sex Attractants - genetics ; Sex Attractants - metabolism ; Sex Attractants - physiology ; Sex glands ; Sex pheromone ; Translation ; Ventilation ; Veterinary Science</subject><ispartof>PloS one, 2012-10, Vol.7 (10), p.e46531-e46531</ispartof><rights>COPYRIGHT 2012 Public Library of Science</rights><rights>2012 Enault et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License: https://creativecommons.org/licenses/by/4.0/ (the “License”), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><rights>Distributed under a Creative Commons Attribution 4.0 International License</rights><rights>2012 Enault et al 2012 Enault et al</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c726t-7d358d705f0cc9be368a185d6a5b92282f6ae5b9ed3454400a2b25bec349e9063</citedby><cites>FETCH-LOGICAL-c726t-7d358d705f0cc9be368a185d6a5b92282f6ae5b9ed3454400a2b25bec349e9063</cites><orcidid>0000-0002-0367-2414 ; 0000-0002-7814-9927 ; 0000-0001-9258-9876 ; 0000-0003-0100-4866</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC3484142/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC3484142/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,727,780,784,864,885,2102,2928,23866,27924,27925,53791,53793,79600,79601</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/23118854$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink><backlink>$$Uhttps://hal.science/hal-00870790$$DView record in HAL$$Hfree_for_read</backlink></links><search><contributor>Ranganathan, Shoba</contributor><creatorcontrib>Enault, Jérémy</creatorcontrib><creatorcontrib>Zatylny-Gaudin, Céline</creatorcontrib><creatorcontrib>Bernay, Benoît</creatorcontrib><creatorcontrib>Lefranc, Benjamin</creatorcontrib><creatorcontrib>Leprince, Jérôme</creatorcontrib><creatorcontrib>Baudy-Floc'h, Michèle</creatorcontrib><creatorcontrib>Henry, Joël</creatorcontrib><title>A complex set of sex pheromones identified in the cuttlefish Sepia officinalis</title><title>PloS one</title><addtitle>PLoS One</addtitle><description>The cephalopod mollusk Sepia officinalis can be considered as a relevant model for studying reproduction strategies associated to seasonal migrations. Using transcriptomic and peptidomic approaches, we aim to identify peptide sex pheromones that are thought to induce the aggregation of mature cuttlefish in their egg-laying areas.
To facilitate the identification of sex pheromones, 576 5'-expressed sequence tags (ESTs) were sequenced from a single cDNA library generated from accessory sex glands of female cuttlefish. Our analysis yielded 223 unique sequences composed of 186 singletons and 37 contigs. Three major redundant ESTs called SPα, SPα' and SPβ were identified as good candidates for putative sex pheromone transcripts and are part of the 87 unique sequences classified as unknown. The alignment of translated SPα and SPα' revealed a high level of conservation, with 98.4% identity. Translation led to a 248-amino acid precursor containing six peptides with multiple putative disulfide bonds. The alignment of SPα-α' with SPβ revealed a partial structural conservation, with 37.3% identity. Translation of SPβ led to a 252-amino acid precursor containing five peptides. The occurrence of a signal peptide on SPα, SPα' and SPβ showed that the peptides were secreted. RT-PCR and mass spectrometry analyses revealed a co-localization of transcripts and expression products in the oviduct gland. Preliminary in vitro experiments performed on gills and penises revealed target organs involved in mating and ventilation.
The analysis of the accessory sex gland transcriptome of Sepia officinalis led to the identification of peptidic sex pheromones. Although preliminary functional tests suggested the involvement of the α3 and β2 peptides in ventilation and mating stimulation, further functional investigations will make it possible to identify the complete set of biological activities expected from waterborne pheromones.</description><subject>Acids</subject><subject>Alignment</subject><subject>Amino Acid Sequence</subject><subject>Amino acids</subject><subject>Animal behavior</subject><subject>Animal Migration - physiology</subject><subject>Animals</subject><subject>Aplysia</subject><subject>Bioinformatics</subject><subject>Biology</subject><subject>Chemical bonds</subject><subject>Chemical Sciences</subject><subject>Communication</subject><subject>Conservation</subject><subject>Decapodiformes - genetics</subject><subject>Decapodiformes - metabolism</subject><subject>Decapodiformes - physiology</subject><subject>Disulfide bonds</subject><subject>Egg laying</subject><subject>Eggs</subject><subject>Expressed sequence tags</subject><subject>Expressed Sequence Tags - metabolism</subject><subject>Female</subject><subject>Gene expression</subject><subject>Gene Expression Profiling</subject><subject>Gene Library</subject><subject>Genomic libraries</subject><subject>Genomics</subject><subject>Gills</subject><subject>Glands</subject><subject>Localization</subject><subject>Mass Spectrometry</subject><subject>Mass spectroscopy</subject><subject>Mating</subject><subject>Molecular Sequence Data</subject><subject>Ontology</subject><subject>Organic chemistry</subject><subject>Organs</subject><subject>Oviduct</subject><subject>Peptides</subject><subject>Peptides - genetics</subject><subject>Peptides - metabolism</subject><subject>Pheromones</subject><subject>Polymerase chain reaction</subject><subject>Proteins</subject><subject>Reproduction - physiology</subject><subject>Seasons</subject><subject>Sepia officinalis</subject><subject>Sequence Analysis, DNA</subject><subject>Sex</subject><subject>Sex Attractants - genetics</subject><subject>Sex Attractants - metabolism</subject><subject>Sex Attractants - physiology</subject><subject>Sex glands</subject><subject>Sex pheromone</subject><subject>Translation</subject><subject>Ventilation</subject><subject>Veterinary Science</subject><issn>1932-6203</issn><issn>1932-6203</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2012</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><sourceid>DOA</sourceid><recordid>eNqNk01v1DAQhiMEoqXwDxBEQkL0sIu_4jgXpFVV6EorKlHgajnOZONVEofYqZZ_X4dNq03VA_LB1uR53xlPPFH0FqMlpin-vLND36p62dkWlggxnlD8LDrFGSULThB9fnQ-iV45t0MooYLzl9EJoRgLkbDT6Psq1rbpatjHDnxsy7Dt466C3jbB2MWmgNab0kARmzb2FcR68L6G0rgqvoHOqCAqjTahFuNeRy9KVTt4M-1n0a-vlz8vrhab62_ri9VmoVPC_SItaCKKFCUl0jrLgXKhsEgKrpI8I0SQkisIRygoSxhDSJGcJDloyjLIEKdn0fuDb1dbJ6dWOIkp4QnHKCOBWB-Iwqqd7HrTqP6vtMrIfwHbb6XqvdE1SEIFwjlPM1EQBjrJixxlWOdYsJLRdMz2Zco25A0UOnSkV_XMdP6lNZXc2ltJmWCYjcWcHwyqR7Kr1UaOMYREitIM3eLAfpqS9fbPAM7LxjgNda1asEO4IyacY07EWNeHR-jTnZiorQqXNW1pQ416NJUrlvIsowzRQC2foMIqoDE6PIXShPhMcD4TBMbD3m_V4Jxc3_z4f_b695z9eMRWoGpfOVsP3tjWzUF2AHVvneuhfOgsRnIckftuyHFE5DQiQfbu-Gc-iO5ngt4Bt0IJLw</recordid><startdate>20121030</startdate><enddate>20121030</enddate><creator>Enault, Jérémy</creator><creator>Zatylny-Gaudin, Céline</creator><creator>Bernay, Benoît</creator><creator>Lefranc, Benjamin</creator><creator>Leprince, Jérôme</creator><creator>Baudy-Floc'h, Michèle</creator><creator>Henry, Joël</creator><general>Public Library of Science</general><general>Public Library of Science (PLoS)</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>IOV</scope><scope>ISR</scope><scope>3V.</scope><scope>7QG</scope><scope>7QL</scope><scope>7QO</scope><scope>7RV</scope><scope>7SN</scope><scope>7SS</scope><scope>7T5</scope><scope>7TG</scope><scope>7TM</scope><scope>7U9</scope><scope>7X2</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>8AO</scope><scope>8C1</scope><scope>8FD</scope><scope>8FE</scope><scope>8FG</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABJCF</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>ARAPS</scope><scope>ATCPS</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BGLVJ</scope><scope>BHPHI</scope><scope>C1K</scope><scope>CCPQU</scope><scope>D1I</scope><scope>DWQXO</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>H94</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>KB.</scope><scope>KB0</scope><scope>KL.</scope><scope>L6V</scope><scope>LK8</scope><scope>M0K</scope><scope>M0S</scope><scope>M1P</scope><scope>M7N</scope><scope>M7P</scope><scope>M7S</scope><scope>NAPCQ</scope><scope>P5Z</scope><scope>P62</scope><scope>P64</scope><scope>PATMY</scope><scope>PDBOC</scope><scope>PIMPY</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>PTHSS</scope><scope>PYCSY</scope><scope>RC3</scope><scope>7X8</scope><scope>1XC</scope><scope>VOOES</scope><scope>5PM</scope><scope>DOA</scope><orcidid>https://orcid.org/0000-0002-0367-2414</orcidid><orcidid>https://orcid.org/0000-0002-7814-9927</orcidid><orcidid>https://orcid.org/0000-0001-9258-9876</orcidid><orcidid>https://orcid.org/0000-0003-0100-4866</orcidid></search><sort><creationdate>20121030</creationdate><title>A complex set of sex pheromones identified in the cuttlefish Sepia officinalis</title><author>Enault, Jérémy ; Zatylny-Gaudin, Céline ; Bernay, Benoît ; Lefranc, Benjamin ; Leprince, Jérôme ; Baudy-Floc'h, Michèle ; Henry, Joël</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c726t-7d358d705f0cc9be368a185d6a5b92282f6ae5b9ed3454400a2b25bec349e9063</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2012</creationdate><topic>Acids</topic><topic>Alignment</topic><topic>Amino Acid Sequence</topic><topic>Amino acids</topic><topic>Animal behavior</topic><topic>Animal Migration - physiology</topic><topic>Animals</topic><topic>Aplysia</topic><topic>Bioinformatics</topic><topic>Biology</topic><topic>Chemical bonds</topic><topic>Chemical Sciences</topic><topic>Communication</topic><topic>Conservation</topic><topic>Decapodiformes - genetics</topic><topic>Decapodiformes - metabolism</topic><topic>Decapodiformes - physiology</topic><topic>Disulfide bonds</topic><topic>Egg laying</topic><topic>Eggs</topic><topic>Expressed sequence tags</topic><topic>Expressed Sequence Tags - metabolism</topic><topic>Female</topic><topic>Gene expression</topic><topic>Gene Expression Profiling</topic><topic>Gene Library</topic><topic>Genomic libraries</topic><topic>Genomics</topic><topic>Gills</topic><topic>Glands</topic><topic>Localization</topic><topic>Mass Spectrometry</topic><topic>Mass spectroscopy</topic><topic>Mating</topic><topic>Molecular Sequence Data</topic><topic>Ontology</topic><topic>Organic chemistry</topic><topic>Organs</topic><topic>Oviduct</topic><topic>Peptides</topic><topic>Peptides - genetics</topic><topic>Peptides - metabolism</topic><topic>Pheromones</topic><topic>Polymerase chain reaction</topic><topic>Proteins</topic><topic>Reproduction - physiology</topic><topic>Seasons</topic><topic>Sepia officinalis</topic><topic>Sequence Analysis, DNA</topic><topic>Sex</topic><topic>Sex Attractants - genetics</topic><topic>Sex Attractants - metabolism</topic><topic>Sex Attractants - physiology</topic><topic>Sex glands</topic><topic>Sex pheromone</topic><topic>Translation</topic><topic>Ventilation</topic><topic>Veterinary Science</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Enault, Jérémy</creatorcontrib><creatorcontrib>Zatylny-Gaudin, Céline</creatorcontrib><creatorcontrib>Bernay, Benoît</creatorcontrib><creatorcontrib>Lefranc, Benjamin</creatorcontrib><creatorcontrib>Leprince, Jérôme</creatorcontrib><creatorcontrib>Baudy-Floc'h, Michèle</creatorcontrib><creatorcontrib>Henry, Joël</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Gale In Context: Opposing Viewpoints</collection><collection>Gale In Context: Science</collection><collection>ProQuest Central (Corporate)</collection><collection>Animal Behavior Abstracts</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Biotechnology Research Abstracts</collection><collection>Nursing & Allied Health Database</collection><collection>Ecology Abstracts</collection><collection>Entomology Abstracts (Full archive)</collection><collection>Immunology Abstracts</collection><collection>Meteorological & Geoastrophysical Abstracts</collection><collection>Nucleic Acids Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>Agricultural Science Collection</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Medical Database (Alumni Edition)</collection><collection>ProQuest Pharma Collection</collection><collection>Public Health Database</collection><collection>Technology Research Database</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Technology Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>Materials Science & Engineering Collection</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest Central UK/Ireland</collection><collection>Advanced Technologies & Aerospace Collection</collection><collection>Agricultural & Environmental Science Collection</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Technology Collection</collection><collection>Natural Science Collection</collection><collection>Environmental Sciences and Pollution Management</collection><collection>ProQuest One Community College</collection><collection>ProQuest Materials Science Collection</collection><collection>ProQuest Central Korea</collection><collection>Engineering Research Database</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Materials Science Database</collection><collection>Nursing & Allied Health Database (Alumni Edition)</collection><collection>Meteorological & Geoastrophysical Abstracts - 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Academic</collection><collection>Hyper Article en Ligne (HAL)</collection><collection>Hyper Article en Ligne (HAL) (Open Access)</collection><collection>PubMed Central (Full Participant titles)</collection><collection>DOAJ Directory of Open Access Journals</collection><jtitle>PloS one</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Enault, Jérémy</au><au>Zatylny-Gaudin, Céline</au><au>Bernay, Benoît</au><au>Lefranc, Benjamin</au><au>Leprince, Jérôme</au><au>Baudy-Floc'h, Michèle</au><au>Henry, Joël</au><au>Ranganathan, Shoba</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>A complex set of sex pheromones identified in the cuttlefish Sepia officinalis</atitle><jtitle>PloS one</jtitle><addtitle>PLoS One</addtitle><date>2012-10-30</date><risdate>2012</risdate><volume>7</volume><issue>10</issue><spage>e46531</spage><epage>e46531</epage><pages>e46531-e46531</pages><issn>1932-6203</issn><eissn>1932-6203</eissn><abstract>The cephalopod mollusk Sepia officinalis can be considered as a relevant model for studying reproduction strategies associated to seasonal migrations. Using transcriptomic and peptidomic approaches, we aim to identify peptide sex pheromones that are thought to induce the aggregation of mature cuttlefish in their egg-laying areas.
To facilitate the identification of sex pheromones, 576 5'-expressed sequence tags (ESTs) were sequenced from a single cDNA library generated from accessory sex glands of female cuttlefish. Our analysis yielded 223 unique sequences composed of 186 singletons and 37 contigs. Three major redundant ESTs called SPα, SPα' and SPβ were identified as good candidates for putative sex pheromone transcripts and are part of the 87 unique sequences classified as unknown. The alignment of translated SPα and SPα' revealed a high level of conservation, with 98.4% identity. Translation led to a 248-amino acid precursor containing six peptides with multiple putative disulfide bonds. The alignment of SPα-α' with SPβ revealed a partial structural conservation, with 37.3% identity. Translation of SPβ led to a 252-amino acid precursor containing five peptides. The occurrence of a signal peptide on SPα, SPα' and SPβ showed that the peptides were secreted. RT-PCR and mass spectrometry analyses revealed a co-localization of transcripts and expression products in the oviduct gland. Preliminary in vitro experiments performed on gills and penises revealed target organs involved in mating and ventilation.
The analysis of the accessory sex gland transcriptome of Sepia officinalis led to the identification of peptidic sex pheromones. Although preliminary functional tests suggested the involvement of the α3 and β2 peptides in ventilation and mating stimulation, further functional investigations will make it possible to identify the complete set of biological activities expected from waterborne pheromones.</abstract><cop>United States</cop><pub>Public Library of Science</pub><pmid>23118854</pmid><doi>10.1371/journal.pone.0046531</doi><tpages>e46531</tpages><orcidid>https://orcid.org/0000-0002-0367-2414</orcidid><orcidid>https://orcid.org/0000-0002-7814-9927</orcidid><orcidid>https://orcid.org/0000-0001-9258-9876</orcidid><orcidid>https://orcid.org/0000-0003-0100-4866</orcidid><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 1932-6203 |
| ispartof | PloS one, 2012-10, Vol.7 (10), p.e46531-e46531 |
| issn | 1932-6203 1932-6203 |
| language | eng |
| recordid | cdi_plos_journals_1326561092 |
| source | MEDLINE; DOAJ Directory of Open Access Journals; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; Public Library of Science (PLoS); PubMed Central; Free Full-Text Journals in Chemistry |
| subjects | Acids Alignment Amino Acid Sequence Amino acids Animal behavior Animal Migration - physiology Animals Aplysia Bioinformatics Biology Chemical bonds Chemical Sciences Communication Conservation Decapodiformes - genetics Decapodiformes - metabolism Decapodiformes - physiology Disulfide bonds Egg laying Eggs Expressed sequence tags Expressed Sequence Tags - metabolism Female Gene expression Gene Expression Profiling Gene Library Genomic libraries Genomics Gills Glands Localization Mass Spectrometry Mass spectroscopy Mating Molecular Sequence Data Ontology Organic chemistry Organs Oviduct Peptides Peptides - genetics Peptides - metabolism Pheromones Polymerase chain reaction Proteins Reproduction - physiology Seasons Sepia officinalis Sequence Analysis, DNA Sex Sex Attractants - genetics Sex Attractants - metabolism Sex Attractants - physiology Sex glands Sex pheromone Translation Ventilation Veterinary Science |
| title | A complex set of sex pheromones identified in the cuttlefish Sepia officinalis |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-05T12%3A34%3A25IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-gale_plos_&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=A%20complex%20set%20of%20sex%20pheromones%20identified%20in%20the%20cuttlefish%20Sepia%20officinalis&rft.jtitle=PloS%20one&rft.au=Enault,%20J%C3%A9r%C3%A9my&rft.date=2012-10-30&rft.volume=7&rft.issue=10&rft.spage=e46531&rft.epage=e46531&rft.pages=e46531-e46531&rft.issn=1932-6203&rft.eissn=1932-6203&rft_id=info:doi/10.1371/journal.pone.0046531&rft_dat=%3Cgale_plos_%3EA476993403%3C/gale_plos_%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=1326561092&rft_id=info:pmid/23118854&rft_galeid=A476993403&rft_doaj_id=oai_doaj_org_article_23801b6798d24ec5bdb091cb184f4376&rfr_iscdi=true |