Establishment of inducible wild type and mutant myocilin-GFP-expressing RGC5 cell lines

Myocilin is a gene linked directly to juvenile- and adult-onset open angle glaucoma. Mutations including Gln368stop (Q368X) and Pro370Leu (P370L) have been identified in patients. The exact role of myocilin and its functional association with glaucoma are still unclear. In the present study, we esta...

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Veröffentlicht in:	PloS one 2012-10, Vol.7 (10), p.e47307-e47307
Hauptverfasser:	Ying, Hongyu, Shen, Xiang, Yue, Beatrice Y J T
Format:	Artikel
Sprache:	eng
Schlagworte:	Aberration Actin Actins - metabolism Analysis Animals Biology Biotechnology Blotting, Western Care and treatment Cell Culture Techniques - methods Cell Line Cell lines Cell migration Cell Movement - drug effects Cloning Cytoskeletal Proteins - metabolism Doxycycline Doxycycline - pharmacology Endothelium Eye Proteins - metabolism Fibers Fluorescence Genes Glaucoma Glycoproteins - metabolism Green Fluorescent Proteins - metabolism Health aspects Humans Immunoblotting Immunoglobulins Kinases Medicine Mutant Proteins - metabolism Mutants Mutation Pathogenesis Permeability Phenotypes Plasmids - genetics Proteins Proteomics Rats Recombinant Fusion Proteins - metabolism rhoA GTP-Binding Protein - metabolism RhoA protein Risk factors Staining and Labeling Stress Fibers - drug effects Stress Fibers - metabolism Tetracycline - pharmacology Tetracyclines Trypsin - metabolism Western blotting
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