High bias gas flows increase lung injury in the ventilated preterm lamb
Mechanical ventilation of preterm babies increases survival but can also cause ventilator-induced lung injury (VILI), leading to the development of bronchopulmonary dysplasia (BPD). It is not known whether shear stress injury from gases flowing into the preterm lung during ventilation contributes to...
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| Veröffentlicht in: | PloS one 2012-10, Vol.7 (10), p.e47044 |
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| creator | Bach, Katinka P Kuschel, Carl A Hooper, Stuart B Bertram, Jean McKnight, Sue Peachey, Shirley E Zahra, Valerie A Flecknoe, Sharon J Oliver, Mark H Wallace, Megan J Bloomfield, Frank H |
| description | Mechanical ventilation of preterm babies increases survival but can also cause ventilator-induced lung injury (VILI), leading to the development of bronchopulmonary dysplasia (BPD). It is not known whether shear stress injury from gases flowing into the preterm lung during ventilation contributes to VILI.
Preterm lambs of 131 days' gestation (term = 147 d) were ventilated for 2 hours with a bias gas flow of 8 L/min (n = 13), 18 L/min (n = 12) or 28 L/min (n = 14). Physiological parameters were measured continuously and lung injury was assessed by measuring mRNA expression of early injury response genes and by histological analysis. Control lung tissue was collected from unventilated age-matched fetuses. Data were analysed by ANOVA with a Tukey post-hoc test when appropriate.
High bias gas flows resulted in higher ventilator pressures, shorter inflation times and decreased ventilator efficiency. The rate of rise of inspiratory gas flow was greatest, and pulmonary mRNA levels of the injury markers, EGR1 and CTGF, were highest in lambs ventilated with bias gas flows of 18 L/min. High bias gas flows resulted in increased cellular proliferation and abnormal deposition of elastin, collagen and myofibroblasts in the lung.
High ventilator bias gas flows resulted in increased lung injury, with up-regulation of acute early response genes and increased histological lung injury. Bias gas flows may, therefore, contribute to VILI and BPD. |
| doi_str_mv | 10.1371/journal.pone.0047044 |
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Preterm lambs of 131 days' gestation (term = 147 d) were ventilated for 2 hours with a bias gas flow of 8 L/min (n = 13), 18 L/min (n = 12) or 28 L/min (n = 14). Physiological parameters were measured continuously and lung injury was assessed by measuring mRNA expression of early injury response genes and by histological analysis. Control lung tissue was collected from unventilated age-matched fetuses. Data were analysed by ANOVA with a Tukey post-hoc test when appropriate.
High bias gas flows resulted in higher ventilator pressures, shorter inflation times and decreased ventilator efficiency. The rate of rise of inspiratory gas flow was greatest, and pulmonary mRNA levels of the injury markers, EGR1 and CTGF, were highest in lambs ventilated with bias gas flows of 18 L/min. High bias gas flows resulted in increased cellular proliferation and abnormal deposition of elastin, collagen and myofibroblasts in the lung.
High ventilator bias gas flows resulted in increased lung injury, with up-regulation of acute early response genes and increased histological lung injury. Bias gas flows may, therefore, contribute to VILI and BPD.</description><identifier>ISSN: 1932-6203</identifier><identifier>EISSN: 1932-6203</identifier><identifier>DOI: 10.1371/journal.pone.0047044</identifier><identifier>PMID: 23056572</identifier><language>eng</language><publisher>United States: Public Library of Science</publisher><subject>Analysis ; Angiogenesis ; Animals ; Animals, Newborn ; Babies ; Bias ; Biology ; Bronchopulmonary Dysplasia - etiology ; Bronchopulmonary Dysplasia - metabolism ; Collagen ; Connective tissue growth factor ; Data processing ; Dysplasia ; EGR-1 protein ; Elastin ; Fetuses ; Gas flow ; Gases ; Gene expression ; Genes ; Genomics ; Gestation ; Hospitals ; Humans ; Infant, Newborn ; Injuries ; Injury analysis ; Lamb ; Lung diseases ; Lungs ; Mechanical stimuli ; Mechanical ventilation ; Medical research ; Medicine ; Natural gas ; Neonates ; Newborn babies ; Physiological aspects ; Premature birth ; Premature infants ; Respiration ; Respiratory system ; RNA ; Shear stress ; Sheep ; Stress, Mechanical ; Variance analysis ; Ventilation ; Ventilator-Induced Lung Injury - etiology ; Ventilator-Induced Lung Injury - metabolism ; Ventilators</subject><ispartof>PloS one, 2012-10, Vol.7 (10), p.e47044</ispartof><rights>COPYRIGHT 2012 Public Library of Science</rights><rights>Bach et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License: https://creativecommons.org/licenses/by/4.0/ (the “License”), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><rights>2012 Bach et al 2012 Bach et al</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c692t-89c337b8da1419fae19734e083e072901bd65cc0c94c700cdb94d272a4daa3ec3</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC3466239/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC3466239/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,723,776,780,860,881,2096,2915,23845,27901,27902,53766,53768,79342,79343</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/23056572$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Bach, Katinka P</creatorcontrib><creatorcontrib>Kuschel, Carl A</creatorcontrib><creatorcontrib>Hooper, Stuart B</creatorcontrib><creatorcontrib>Bertram, Jean</creatorcontrib><creatorcontrib>McKnight, Sue</creatorcontrib><creatorcontrib>Peachey, Shirley E</creatorcontrib><creatorcontrib>Zahra, Valerie A</creatorcontrib><creatorcontrib>Flecknoe, Sharon J</creatorcontrib><creatorcontrib>Oliver, Mark H</creatorcontrib><creatorcontrib>Wallace, Megan J</creatorcontrib><creatorcontrib>Bloomfield, Frank H</creatorcontrib><title>High bias gas flows increase lung injury in the ventilated preterm lamb</title><title>PloS one</title><addtitle>PLoS One</addtitle><description>Mechanical ventilation of preterm babies increases survival but can also cause ventilator-induced lung injury (VILI), leading to the development of bronchopulmonary dysplasia (BPD). It is not known whether shear stress injury from gases flowing into the preterm lung during ventilation contributes to VILI.
Preterm lambs of 131 days' gestation (term = 147 d) were ventilated for 2 hours with a bias gas flow of 8 L/min (n = 13), 18 L/min (n = 12) or 28 L/min (n = 14). Physiological parameters were measured continuously and lung injury was assessed by measuring mRNA expression of early injury response genes and by histological analysis. Control lung tissue was collected from unventilated age-matched fetuses. Data were analysed by ANOVA with a Tukey post-hoc test when appropriate.
High bias gas flows resulted in higher ventilator pressures, shorter inflation times and decreased ventilator efficiency. The rate of rise of inspiratory gas flow was greatest, and pulmonary mRNA levels of the injury markers, EGR1 and CTGF, were highest in lambs ventilated with bias gas flows of 18 L/min. High bias gas flows resulted in increased cellular proliferation and abnormal deposition of elastin, collagen and myofibroblasts in the lung.
High ventilator bias gas flows resulted in increased lung injury, with up-regulation of acute early response genes and increased histological lung injury. Bias gas flows may, therefore, contribute to VILI and BPD.</description><subject>Analysis</subject><subject>Angiogenesis</subject><subject>Animals</subject><subject>Animals, Newborn</subject><subject>Babies</subject><subject>Bias</subject><subject>Biology</subject><subject>Bronchopulmonary Dysplasia - etiology</subject><subject>Bronchopulmonary Dysplasia - metabolism</subject><subject>Collagen</subject><subject>Connective tissue growth factor</subject><subject>Data processing</subject><subject>Dysplasia</subject><subject>EGR-1 protein</subject><subject>Elastin</subject><subject>Fetuses</subject><subject>Gas flow</subject><subject>Gases</subject><subject>Gene expression</subject><subject>Genes</subject><subject>Genomics</subject><subject>Gestation</subject><subject>Hospitals</subject><subject>Humans</subject><subject>Infant, Newborn</subject><subject>Injuries</subject><subject>Injury analysis</subject><subject>Lamb</subject><subject>Lung diseases</subject><subject>Lungs</subject><subject>Mechanical stimuli</subject><subject>Mechanical ventilation</subject><subject>Medical research</subject><subject>Medicine</subject><subject>Natural gas</subject><subject>Neonates</subject><subject>Newborn babies</subject><subject>Physiological aspects</subject><subject>Premature birth</subject><subject>Premature infants</subject><subject>Respiration</subject><subject>Respiratory system</subject><subject>RNA</subject><subject>Shear stress</subject><subject>Sheep</subject><subject>Stress, Mechanical</subject><subject>Variance analysis</subject><subject>Ventilation</subject><subject>Ventilator-Induced Lung Injury - etiology</subject><subject>Ventilator-Induced Lung Injury - metabolism</subject><subject>Ventilators</subject><issn>1932-6203</issn><issn>1932-6203</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2012</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>BENPR</sourceid><sourceid>DOA</sourceid><recordid>eNqNkttu1DAQhiMEoqXwBggiISG42MWnxPENUlVBu1KlSpxurYkzyXrljRc7KfTtcdm02qBeYMvyId_89kz-LHtJyZJyST9s_Bh6cMud73FJiJBEiEfZMVWcLUpG-OOD9VH2LMYNIQWvyvJpdsQ4KcpCsuPs_MJ267y2EPMujdb5XzG3vQkIEXM39l3abcZwk6Z8WGN-jf1gHQzY5LuAA4Zt7mBbP8-etOAivpjmk-z750_fzi4Wl1fnq7PTy4UpFRsWlTKcy7pqgAqqWkCqJBdIKo5EMkVo3ZSFMcQoYSQhpqmVaJhkIBoAjoafZK_3ujvno55qEDXlrCyKSnCSiNWeaDxs9C7YLYQb7cHqvwc-dBrCYI1DzaAFRRpBKjCibhgoUddG8baSRErEpPVxum2st9iYlHsANxOdf-ntWnf-WnNRloyrJPBuEgj-54hx0FsbDToHPfoxvTu1quQVlwl98w_6cHYT1UFKwPatT_eaW1F9WgieQEVpopYPUKk3uLUmGaa16XwW8H4WkJgBfw8djDHq1dcv_89e_Zizbw_YNYIb1tG7cbC-j3NQ7EETfIwB2_siU6Jv_X5XDX3rdz35PYW9OvxB90F3Bud_AFkW-co</recordid><startdate>20121008</startdate><enddate>20121008</enddate><creator>Bach, Katinka P</creator><creator>Kuschel, Carl A</creator><creator>Hooper, Stuart B</creator><creator>Bertram, Jean</creator><creator>McKnight, Sue</creator><creator>Peachey, Shirley E</creator><creator>Zahra, Valerie A</creator><creator>Flecknoe, Sharon J</creator><creator>Oliver, Mark H</creator><creator>Wallace, Megan J</creator><creator>Bloomfield, Frank H</creator><general>Public Library of Science</general><general>Public Library of Science (PLoS)</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>IOV</scope><scope>ISR</scope><scope>3V.</scope><scope>7QG</scope><scope>7QL</scope><scope>7QO</scope><scope>7RV</scope><scope>7SN</scope><scope>7SS</scope><scope>7T5</scope><scope>7TG</scope><scope>7TM</scope><scope>7U9</scope><scope>7X2</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>8AO</scope><scope>8C1</scope><scope>8FD</scope><scope>8FE</scope><scope>8FG</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABJCF</scope><scope>ABUWG</scope><scope>AEUYN</scope><scope>AFKRA</scope><scope>ARAPS</scope><scope>ATCPS</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BGLVJ</scope><scope>BHPHI</scope><scope>C1K</scope><scope>CCPQU</scope><scope>D1I</scope><scope>DWQXO</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>H94</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>KB.</scope><scope>KB0</scope><scope>KL.</scope><scope>L6V</scope><scope>LK8</scope><scope>M0K</scope><scope>M0S</scope><scope>M1P</scope><scope>M7N</scope><scope>M7P</scope><scope>M7S</scope><scope>NAPCQ</scope><scope>P5Z</scope><scope>P62</scope><scope>P64</scope><scope>PATMY</scope><scope>PDBOC</scope><scope>PIMPY</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>PTHSS</scope><scope>PYCSY</scope><scope>RC3</scope><scope>7X8</scope><scope>5PM</scope><scope>DOA</scope></search><sort><creationdate>20121008</creationdate><title>High bias gas flows increase lung injury in the ventilated preterm lamb</title><author>Bach, Katinka P ; Kuschel, Carl A ; Hooper, Stuart B ; Bertram, Jean ; McKnight, Sue ; Peachey, Shirley E ; Zahra, Valerie A ; Flecknoe, Sharon J ; Oliver, Mark H ; Wallace, Megan J ; Bloomfield, Frank H</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c692t-89c337b8da1419fae19734e083e072901bd65cc0c94c700cdb94d272a4daa3ec3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2012</creationdate><topic>Analysis</topic><topic>Angiogenesis</topic><topic>Animals</topic><topic>Animals, Newborn</topic><topic>Babies</topic><topic>Bias</topic><topic>Biology</topic><topic>Bronchopulmonary Dysplasia - etiology</topic><topic>Bronchopulmonary Dysplasia - metabolism</topic><topic>Collagen</topic><topic>Connective tissue growth factor</topic><topic>Data processing</topic><topic>Dysplasia</topic><topic>EGR-1 protein</topic><topic>Elastin</topic><topic>Fetuses</topic><topic>Gas flow</topic><topic>Gases</topic><topic>Gene expression</topic><topic>Genes</topic><topic>Genomics</topic><topic>Gestation</topic><topic>Hospitals</topic><topic>Humans</topic><topic>Infant, Newborn</topic><topic>Injuries</topic><topic>Injury analysis</topic><topic>Lamb</topic><topic>Lung diseases</topic><topic>Lungs</topic><topic>Mechanical stimuli</topic><topic>Mechanical ventilation</topic><topic>Medical research</topic><topic>Medicine</topic><topic>Natural gas</topic><topic>Neonates</topic><topic>Newborn babies</topic><topic>Physiological aspects</topic><topic>Premature birth</topic><topic>Premature infants</topic><topic>Respiration</topic><topic>Respiratory system</topic><topic>RNA</topic><topic>Shear stress</topic><topic>Sheep</topic><topic>Stress, Mechanical</topic><topic>Variance analysis</topic><topic>Ventilation</topic><topic>Ventilator-Induced Lung Injury - 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Academic</collection><collection>PubMed Central (Full Participant titles)</collection><collection>DOAJ Directory of Open Access Journals</collection><jtitle>PloS one</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Bach, Katinka P</au><au>Kuschel, Carl A</au><au>Hooper, Stuart B</au><au>Bertram, Jean</au><au>McKnight, Sue</au><au>Peachey, Shirley E</au><au>Zahra, Valerie A</au><au>Flecknoe, Sharon J</au><au>Oliver, Mark H</au><au>Wallace, Megan J</au><au>Bloomfield, Frank H</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>High bias gas flows increase lung injury in the ventilated preterm lamb</atitle><jtitle>PloS one</jtitle><addtitle>PLoS One</addtitle><date>2012-10-08</date><risdate>2012</risdate><volume>7</volume><issue>10</issue><spage>e47044</spage><pages>e47044-</pages><issn>1932-6203</issn><eissn>1932-6203</eissn><abstract>Mechanical ventilation of preterm babies increases survival but can also cause ventilator-induced lung injury (VILI), leading to the development of bronchopulmonary dysplasia (BPD). It is not known whether shear stress injury from gases flowing into the preterm lung during ventilation contributes to VILI.
Preterm lambs of 131 days' gestation (term = 147 d) were ventilated for 2 hours with a bias gas flow of 8 L/min (n = 13), 18 L/min (n = 12) or 28 L/min (n = 14). Physiological parameters were measured continuously and lung injury was assessed by measuring mRNA expression of early injury response genes and by histological analysis. Control lung tissue was collected from unventilated age-matched fetuses. Data were analysed by ANOVA with a Tukey post-hoc test when appropriate.
High bias gas flows resulted in higher ventilator pressures, shorter inflation times and decreased ventilator efficiency. The rate of rise of inspiratory gas flow was greatest, and pulmonary mRNA levels of the injury markers, EGR1 and CTGF, were highest in lambs ventilated with bias gas flows of 18 L/min. High bias gas flows resulted in increased cellular proliferation and abnormal deposition of elastin, collagen and myofibroblasts in the lung.
High ventilator bias gas flows resulted in increased lung injury, with up-regulation of acute early response genes and increased histological lung injury. Bias gas flows may, therefore, contribute to VILI and BPD.</abstract><cop>United States</cop><pub>Public Library of Science</pub><pmid>23056572</pmid><doi>10.1371/journal.pone.0047044</doi><tpages>e47044</tpages><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 1932-6203 |
| ispartof | PloS one, 2012-10, Vol.7 (10), p.e47044 |
| issn | 1932-6203 1932-6203 |
| language | eng |
| recordid | cdi_plos_journals_1326558430 |
| source | MEDLINE; DOAJ Directory of Open Access Journals; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; PubMed Central; Free Full-Text Journals in Chemistry; Public Library of Science (PLoS) |
| subjects | Analysis Angiogenesis Animals Animals, Newborn Babies Bias Biology Bronchopulmonary Dysplasia - etiology Bronchopulmonary Dysplasia - metabolism Collagen Connective tissue growth factor Data processing Dysplasia EGR-1 protein Elastin Fetuses Gas flow Gases Gene expression Genes Genomics Gestation Hospitals Humans Infant, Newborn Injuries Injury analysis Lamb Lung diseases Lungs Mechanical stimuli Mechanical ventilation Medical research Medicine Natural gas Neonates Newborn babies Physiological aspects Premature birth Premature infants Respiration Respiratory system RNA Shear stress Sheep Stress, Mechanical Variance analysis Ventilation Ventilator-Induced Lung Injury - etiology Ventilator-Induced Lung Injury - metabolism Ventilators |
| title | High bias gas flows increase lung injury in the ventilated preterm lamb |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-02-05T23%3A59%3A16IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-gale_plos_&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=High%20bias%20gas%20flows%20increase%20lung%20injury%20in%20the%20ventilated%20preterm%20lamb&rft.jtitle=PloS%20one&rft.au=Bach,%20Katinka%20P&rft.date=2012-10-08&rft.volume=7&rft.issue=10&rft.spage=e47044&rft.pages=e47044-&rft.issn=1932-6203&rft.eissn=1932-6203&rft_id=info:doi/10.1371/journal.pone.0047044&rft_dat=%3Cgale_plos_%3EA543326911%3C/gale_plos_%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=1326558430&rft_id=info:pmid/23056572&rft_galeid=A543326911&rft_doaj_id=oai_doaj_org_article_2afa90d408ac4bd2a94bbc93f87077ee&rfr_iscdi=true |