Altered ratio of IFN-γ/IL-10 in patients with drug resistant Mycobacterium tuberculosis and HIV- Tuberculosis Immune Reconstitution Inflammatory Syndrome
We have described a clinical relationship between HIV-Tuberculosis Immune Reconstitution Inflammatory Syndrome (TB-IRIS) and anti-tubercular drug resistance. Here we studied the immune response of TB-IRIS patients from whom a drug-resistant (n = 11) or drug-susceptible (n = 25) Mycobacterium tubercu...
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| Veröffentlicht in: | PloS one 2012-10, Vol.7 (10), p.e46481-e46481 |
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| creator | Skolimowska, Keira H Rangaka, Molebogeng X Meintjes, Graeme Pepper, Dominique J Seldon, Ronnett Matthews, Kerryn Wilkinson, Robert J Wilkinson, Katalin A |
| description | We have described a clinical relationship between HIV-Tuberculosis Immune Reconstitution Inflammatory Syndrome (TB-IRIS) and anti-tubercular drug resistance. Here we studied the immune response of TB-IRIS patients from whom a drug-resistant (n = 11) or drug-susceptible (n = 25) Mycobacterium tuberculosis (MTB) strain was isolated after presenting with TB-IRIS. ELISpot analysis and multiplex cytokine analysis of the supernatant collected from peripheral blood mononuclear cells stimulated overnight with the heat-killed H37Rv MTB laboratory strain was used. Although there was no statistical difference in IFN-gamma ELISpot responses between the two groups, the results point towards higher bacterial load in the drug-resistant patients, possibly due to failed therapy. The ratio between secreted IFN-gamma/IL-10 and IL-2/IL-10 was significantly lower in TB-IRIS patients in whom the cause of TB was a drug-resistant strain compared to those with a fully sensitive strain (p = 0.02). Since host immune responses are dependent on the bacterial load, we hypothesise that the impaired cytokine balance is likely to be caused by the poorly controlled bacterial growth in these patients. |
| doi_str_mv | 10.1371/journal.pone.0046481 |
| format | Article |
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Here we studied the immune response of TB-IRIS patients from whom a drug-resistant (n = 11) or drug-susceptible (n = 25) Mycobacterium tuberculosis (MTB) strain was isolated after presenting with TB-IRIS. ELISpot analysis and multiplex cytokine analysis of the supernatant collected from peripheral blood mononuclear cells stimulated overnight with the heat-killed H37Rv MTB laboratory strain was used. Although there was no statistical difference in IFN-gamma ELISpot responses between the two groups, the results point towards higher bacterial load in the drug-resistant patients, possibly due to failed therapy. The ratio between secreted IFN-gamma/IL-10 and IL-2/IL-10 was significantly lower in TB-IRIS patients in whom the cause of TB was a drug-resistant strain compared to those with a fully sensitive strain (p = 0.02). 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Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><rights>2012 Skolimowska et al 2012 Skolimowska et al</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c526t-265127533be9acf04747fc2d5a9b249b1963abfa91671eb5da413b3b11db4cce3</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC3468619/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC3468619/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,723,776,780,860,881,2096,2915,23845,27901,27902,53766,53768,79343,79344</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/23071578$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><contributor>Hoshino, Yoshihiko</contributor><creatorcontrib>Skolimowska, Keira H</creatorcontrib><creatorcontrib>Rangaka, Molebogeng X</creatorcontrib><creatorcontrib>Meintjes, Graeme</creatorcontrib><creatorcontrib>Pepper, Dominique J</creatorcontrib><creatorcontrib>Seldon, Ronnett</creatorcontrib><creatorcontrib>Matthews, Kerryn</creatorcontrib><creatorcontrib>Wilkinson, Robert J</creatorcontrib><creatorcontrib>Wilkinson, Katalin A</creatorcontrib><title>Altered ratio of IFN-γ/IL-10 in patients with drug resistant Mycobacterium tuberculosis and HIV- Tuberculosis Immune Reconstitution Inflammatory Syndrome</title><title>PloS one</title><addtitle>PLoS One</addtitle><description>We have described a clinical relationship between HIV-Tuberculosis Immune Reconstitution Inflammatory Syndrome (TB-IRIS) and anti-tubercular drug resistance. Here we studied the immune response of TB-IRIS patients from whom a drug-resistant (n = 11) or drug-susceptible (n = 25) Mycobacterium tuberculosis (MTB) strain was isolated after presenting with TB-IRIS. ELISpot analysis and multiplex cytokine analysis of the supernatant collected from peripheral blood mononuclear cells stimulated overnight with the heat-killed H37Rv MTB laboratory strain was used. Although there was no statistical difference in IFN-gamma ELISpot responses between the two groups, the results point towards higher bacterial load in the drug-resistant patients, possibly due to failed therapy. The ratio between secreted IFN-gamma/IL-10 and IL-2/IL-10 was significantly lower in TB-IRIS patients in whom the cause of TB was a drug-resistant strain compared to those with a fully sensitive strain (p = 0.02). Since host immune responses are dependent on the bacterial load, we hypothesise that the impaired cytokine balance is likely to be caused by the poorly controlled bacterial growth in these patients.</description><subject>Adult</subject><subject>Antigens</subject><subject>Antiretroviral drugs</subject><subject>Bacteria</subject><subject>Biology</subject><subject>Cytokines</subject><subject>Drug resistance</subject><subject>Enzyme-Linked Immunosorbent Assay</subject><subject>Epidemics</subject><subject>Female</subject><subject>Garra</subject><subject>HIV</subject><subject>HIV Infections - blood</subject><subject>Hospitals</subject><subject>Human immunodeficiency virus</subject><subject>Humans</subject><subject>Immune reconstitution</subject><subject>Immune Reconstitution Inflammatory Syndrome - blood</subject><subject>Immune response</subject><subject>Immune system</subject><subject>Immunology</subject><subject>Infectious diseases</subject><subject>Inflammation</subject><subject>Interferon</subject><subject>Interferon-gamma - 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Here we studied the immune response of TB-IRIS patients from whom a drug-resistant (n = 11) or drug-susceptible (n = 25) Mycobacterium tuberculosis (MTB) strain was isolated after presenting with TB-IRIS. ELISpot analysis and multiplex cytokine analysis of the supernatant collected from peripheral blood mononuclear cells stimulated overnight with the heat-killed H37Rv MTB laboratory strain was used. Although there was no statistical difference in IFN-gamma ELISpot responses between the two groups, the results point towards higher bacterial load in the drug-resistant patients, possibly due to failed therapy. The ratio between secreted IFN-gamma/IL-10 and IL-2/IL-10 was significantly lower in TB-IRIS patients in whom the cause of TB was a drug-resistant strain compared to those with a fully sensitive strain (p = 0.02). Since host immune responses are dependent on the bacterial load, we hypothesise that the impaired cytokine balance is likely to be caused by the poorly controlled bacterial growth in these patients.</abstract><cop>United States</cop><pub>Public Library of Science</pub><pmid>23071578</pmid><doi>10.1371/journal.pone.0046481</doi><oa>free_for_read</oa></addata></record> |
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| subjects | Adult Antigens Antiretroviral drugs Bacteria Biology Cytokines Drug resistance Enzyme-Linked Immunosorbent Assay Epidemics Female Garra HIV HIV Infections - blood Hospitals Human immunodeficiency virus Humans Immune reconstitution Immune Reconstitution Inflammatory Syndrome - blood Immune response Immune system Immunology Infectious diseases Inflammation Interferon Interferon-gamma - blood Interleukin 10 Interleukin 2 Interleukin-10 - blood Laboratories Leukocytes (mononuclear) Load resistance Lymphocytes Male Medical research Medicine Multidrug resistant organisms Multiplexing Mycobacterium tuberculosis Patients Peripheral blood mononuclear cells Tuberculosis Tuberculosis, Multidrug-Resistant - blood γ-Interferon |
| title | Altered ratio of IFN-γ/IL-10 in patients with drug resistant Mycobacterium tuberculosis and HIV- Tuberculosis Immune Reconstitution Inflammatory Syndrome |
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