Combined micro-PET/micro-CT imaging of lung tumours in SPC-raf and SPC-myc transgenic mice
SPC-raf and SPC-myc transgenic mice develop disseminated and circumscribed lung adenocarcinoma respectively, allowing for assessment of carcinogenesis and treatment strategies. The purpose of this study was to investigate the technical feasibility, the correlation of initial findings to histology an...
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| Veröffentlicht in: | PloS one 2012-09, Vol.7 (9), p.e44427 |
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| description | SPC-raf and SPC-myc transgenic mice develop disseminated and circumscribed lung adenocarcinoma respectively, allowing for assessment of carcinogenesis and treatment strategies. The purpose of this study was to investigate the technical feasibility, the correlation of initial findings to histology and the administered radiation dose of combined micro-PET/micro-CT in these animal models.
14 C57BL/6 mice (4 nontransgenic, 4 SPC-raf transgenic, 6 SPC-myc transgenic) were examined using micro-CT and (18)F-Fluoro-deoxyglucose micro-PET in-vivo. Micro-PET data was corrected for random events and scatter prior to reconstruction with a 3D-FORE/2D-OSEM iterative algorithm. Rigid micro-PET/micro-CT registration was performed. Tumour-to-non-tumour ratios were calculated for different lung regions and focal lesions. Diffuse tumour growth was quantified using a semiautomated micro-CT segmentation routine reported earlier. Regional histologic tumour load was assessed using a 4-point rating scale. Gamma radiation dose was determined using thermoluminescence dosimeters.
Micro-CT allowed visualisation of diffuse and circumscribed tumours in SPC-raf and SPC-myc transgenic animals along with morphology, while micro-PET provided information on metabolism, but lacked morphologic detail. Mean tumour-to-non-tumour ratio was 2.47 for circumscribed lesions. No significant correlation could be shown between histological tumour load and tumour-to-nontumour ratio for diffuse tumours in SPC-raf transgenic animals. Calculation of the expected dose based on gamma dosimetry yielded approximately 140 mGy/micro-PET examination additional to approximately 200 mGy due to micro-CT.
Combined micro-PET/micro-CT imaging allows for in-vivo assessment of lung tumours in SPC-raf and SPC-myc transgenic mice. The technique has potential for the evaluation of carcinogenesis and treatment strategies in circumscribed lung tumours. |
| doi_str_mv | 10.1371/journal.pone.0044427 |
| format | Article |
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14 C57BL/6 mice (4 nontransgenic, 4 SPC-raf transgenic, 6 SPC-myc transgenic) were examined using micro-CT and (18)F-Fluoro-deoxyglucose micro-PET in-vivo. Micro-PET data was corrected for random events and scatter prior to reconstruction with a 3D-FORE/2D-OSEM iterative algorithm. Rigid micro-PET/micro-CT registration was performed. Tumour-to-non-tumour ratios were calculated for different lung regions and focal lesions. Diffuse tumour growth was quantified using a semiautomated micro-CT segmentation routine reported earlier. Regional histologic tumour load was assessed using a 4-point rating scale. Gamma radiation dose was determined using thermoluminescence dosimeters.
Micro-CT allowed visualisation of diffuse and circumscribed tumours in SPC-raf and SPC-myc transgenic animals along with morphology, while micro-PET provided information on metabolism, but lacked morphologic detail. Mean tumour-to-non-tumour ratio was 2.47 for circumscribed lesions. No significant correlation could be shown between histological tumour load and tumour-to-nontumour ratio for diffuse tumours in SPC-raf transgenic animals. Calculation of the expected dose based on gamma dosimetry yielded approximately 140 mGy/micro-PET examination additional to approximately 200 mGy due to micro-CT.
Combined micro-PET/micro-CT imaging allows for in-vivo assessment of lung tumours in SPC-raf and SPC-myc transgenic mice. The technique has potential for the evaluation of carcinogenesis and treatment strategies in circumscribed lung tumours.</description><identifier>ISSN: 1932-6203</identifier><identifier>EISSN: 1932-6203</identifier><identifier>DOI: 10.1371/journal.pone.0044427</identifier><identifier>PMID: 23028537</identifier><language>eng</language><publisher>United States: Public Library of Science</publisher><subject>Adenocarcinoma ; Animal models ; Animals ; Biology ; Cancer therapies ; Carcinogenesis ; Carcinogens ; Computed tomography ; Correlation ; Deoxyglucose ; Dosimeters ; Dosimetry ; Drug dosages ; Feasibility studies ; Gamma radiation ; Gamma rays ; Health physics ; Histology ; Humans ; Image processing ; Image segmentation ; Iterative algorithms ; Iterative methods ; Laboratory animals ; Lesions ; Lung cancer ; Lung Neoplasms - diagnostic imaging ; Lung Neoplasms - genetics ; Lung Neoplasms - metabolism ; Lung Neoplasms - pathology ; Lungs ; Medical imaging ; Medical schools ; Medicine ; Metabolism ; Mice ; Mice, Inbred C57BL ; Mice, Transgenic ; Morphology ; Multimodal Imaging ; Physics ; Positron emission ; Positron-Emission Tomography ; Pressure transducers ; Proto-Oncogene Proteins c-myc - genetics ; Proto-Oncogene Proteins c-myc - metabolism ; Pulmonary Surfactant-Associated Proteins ; Radiation ; Radiation dosage ; raf Kinases - genetics ; raf Kinases - metabolism ; Raf protein ; Regional analysis ; Rodents ; Segmentation ; Thermoluminescence ; Tomography ; Tomography, X-Ray Computed ; Transgenic animals ; Transgenic mice ; Tumors ; Veterinary medicine ; Veterinary Science ; γ Radiation</subject><ispartof>PloS one, 2012-09, Vol.7 (9), p.e44427</ispartof><rights>Rodt et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License: https://creativecommons.org/licenses/by/4.0/ (the “License”), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><rights>2012 Rodt et al 2012 Rodt et al</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c526t-131edb38b9106b61007b2980222ddceed83040ea10e7f56a375c8cd6857e71273</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC3448619/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC3448619/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,723,776,780,860,881,2096,2915,23845,27901,27902,53766,53768,79342,79343</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/23028537$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Rodt, Thomas</creatorcontrib><creatorcontrib>Luepke, Matthias</creatorcontrib><creatorcontrib>Boehm, Claudia</creatorcontrib><creatorcontrib>Hueper, Katja</creatorcontrib><creatorcontrib>Halter, Roman</creatorcontrib><creatorcontrib>Glage, Silke</creatorcontrib><creatorcontrib>Hoy, Ludwig</creatorcontrib><creatorcontrib>Wacker, Frank</creatorcontrib><creatorcontrib>Borlak, Juergen</creatorcontrib><creatorcontrib>von Falck, Christian</creatorcontrib><title>Combined micro-PET/micro-CT imaging of lung tumours in SPC-raf and SPC-myc transgenic mice</title><title>PloS one</title><addtitle>PLoS One</addtitle><description>SPC-raf and SPC-myc transgenic mice develop disseminated and circumscribed lung adenocarcinoma respectively, allowing for assessment of carcinogenesis and treatment strategies. The purpose of this study was to investigate the technical feasibility, the correlation of initial findings to histology and the administered radiation dose of combined micro-PET/micro-CT in these animal models.
14 C57BL/6 mice (4 nontransgenic, 4 SPC-raf transgenic, 6 SPC-myc transgenic) were examined using micro-CT and (18)F-Fluoro-deoxyglucose micro-PET in-vivo. Micro-PET data was corrected for random events and scatter prior to reconstruction with a 3D-FORE/2D-OSEM iterative algorithm. Rigid micro-PET/micro-CT registration was performed. Tumour-to-non-tumour ratios were calculated for different lung regions and focal lesions. Diffuse tumour growth was quantified using a semiautomated micro-CT segmentation routine reported earlier. Regional histologic tumour load was assessed using a 4-point rating scale. Gamma radiation dose was determined using thermoluminescence dosimeters.
Micro-CT allowed visualisation of diffuse and circumscribed tumours in SPC-raf and SPC-myc transgenic animals along with morphology, while micro-PET provided information on metabolism, but lacked morphologic detail. Mean tumour-to-non-tumour ratio was 2.47 for circumscribed lesions. No significant correlation could be shown between histological tumour load and tumour-to-nontumour ratio for diffuse tumours in SPC-raf transgenic animals. Calculation of the expected dose based on gamma dosimetry yielded approximately 140 mGy/micro-PET examination additional to approximately 200 mGy due to micro-CT.
Combined micro-PET/micro-CT imaging allows for in-vivo assessment of lung tumours in SPC-raf and SPC-myc transgenic mice. The technique has potential for the evaluation of carcinogenesis and treatment strategies in circumscribed lung tumours.</description><subject>Adenocarcinoma</subject><subject>Animal models</subject><subject>Animals</subject><subject>Biology</subject><subject>Cancer therapies</subject><subject>Carcinogenesis</subject><subject>Carcinogens</subject><subject>Computed tomography</subject><subject>Correlation</subject><subject>Deoxyglucose</subject><subject>Dosimeters</subject><subject>Dosimetry</subject><subject>Drug dosages</subject><subject>Feasibility studies</subject><subject>Gamma radiation</subject><subject>Gamma rays</subject><subject>Health physics</subject><subject>Histology</subject><subject>Humans</subject><subject>Image processing</subject><subject>Image segmentation</subject><subject>Iterative algorithms</subject><subject>Iterative methods</subject><subject>Laboratory animals</subject><subject>Lesions</subject><subject>Lung cancer</subject><subject>Lung Neoplasms - diagnostic imaging</subject><subject>Lung Neoplasms - genetics</subject><subject>Lung Neoplasms - metabolism</subject><subject>Lung Neoplasms - pathology</subject><subject>Lungs</subject><subject>Medical imaging</subject><subject>Medical schools</subject><subject>Medicine</subject><subject>Metabolism</subject><subject>Mice</subject><subject>Mice, Inbred C57BL</subject><subject>Mice, Transgenic</subject><subject>Morphology</subject><subject>Multimodal Imaging</subject><subject>Physics</subject><subject>Positron emission</subject><subject>Positron-Emission Tomography</subject><subject>Pressure transducers</subject><subject>Proto-Oncogene Proteins c-myc - genetics</subject><subject>Proto-Oncogene Proteins c-myc - metabolism</subject><subject>Pulmonary Surfactant-Associated Proteins</subject><subject>Radiation</subject><subject>Radiation dosage</subject><subject>raf Kinases - genetics</subject><subject>raf Kinases - metabolism</subject><subject>Raf protein</subject><subject>Regional analysis</subject><subject>Rodents</subject><subject>Segmentation</subject><subject>Thermoluminescence</subject><subject>Tomography</subject><subject>Tomography, X-Ray Computed</subject><subject>Transgenic animals</subject><subject>Transgenic mice</subject><subject>Tumors</subject><subject>Veterinary medicine</subject><subject>Veterinary Science</subject><subject>γ 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micro-PET/micro-CT imaging of lung tumours in SPC-raf and SPC-myc transgenic mice</title><author>Rodt, Thomas ; Luepke, Matthias ; Boehm, Claudia ; Hueper, Katja ; Halter, Roman ; Glage, Silke ; Hoy, Ludwig ; Wacker, Frank ; Borlak, Juergen ; von Falck, Christian</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c526t-131edb38b9106b61007b2980222ddceed83040ea10e7f56a375c8cd6857e71273</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2012</creationdate><topic>Adenocarcinoma</topic><topic>Animal models</topic><topic>Animals</topic><topic>Biology</topic><topic>Cancer therapies</topic><topic>Carcinogenesis</topic><topic>Carcinogens</topic><topic>Computed tomography</topic><topic>Correlation</topic><topic>Deoxyglucose</topic><topic>Dosimeters</topic><topic>Dosimetry</topic><topic>Drug dosages</topic><topic>Feasibility studies</topic><topic>Gamma radiation</topic><topic>Gamma rays</topic><topic>Health 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micro-PET/micro-CT imaging of lung tumours in SPC-raf and SPC-myc transgenic mice</atitle><jtitle>PloS one</jtitle><addtitle>PLoS One</addtitle><date>2012-09-21</date><risdate>2012</risdate><volume>7</volume><issue>9</issue><spage>e44427</spage><pages>e44427-</pages><issn>1932-6203</issn><eissn>1932-6203</eissn><abstract>SPC-raf and SPC-myc transgenic mice develop disseminated and circumscribed lung adenocarcinoma respectively, allowing for assessment of carcinogenesis and treatment strategies. The purpose of this study was to investigate the technical feasibility, the correlation of initial findings to histology and the administered radiation dose of combined micro-PET/micro-CT in these animal models.
14 C57BL/6 mice (4 nontransgenic, 4 SPC-raf transgenic, 6 SPC-myc transgenic) were examined using micro-CT and (18)F-Fluoro-deoxyglucose micro-PET in-vivo. Micro-PET data was corrected for random events and scatter prior to reconstruction with a 3D-FORE/2D-OSEM iterative algorithm. Rigid micro-PET/micro-CT registration was performed. Tumour-to-non-tumour ratios were calculated for different lung regions and focal lesions. Diffuse tumour growth was quantified using a semiautomated micro-CT segmentation routine reported earlier. Regional histologic tumour load was assessed using a 4-point rating scale. Gamma radiation dose was determined using thermoluminescence dosimeters.
Micro-CT allowed visualisation of diffuse and circumscribed tumours in SPC-raf and SPC-myc transgenic animals along with morphology, while micro-PET provided information on metabolism, but lacked morphologic detail. Mean tumour-to-non-tumour ratio was 2.47 for circumscribed lesions. No significant correlation could be shown between histological tumour load and tumour-to-nontumour ratio for diffuse tumours in SPC-raf transgenic animals. Calculation of the expected dose based on gamma dosimetry yielded approximately 140 mGy/micro-PET examination additional to approximately 200 mGy due to micro-CT.
Combined micro-PET/micro-CT imaging allows for in-vivo assessment of lung tumours in SPC-raf and SPC-myc transgenic mice. The technique has potential for the evaluation of carcinogenesis and treatment strategies in circumscribed lung tumours.</abstract><cop>United States</cop><pub>Public Library of Science</pub><pmid>23028537</pmid><doi>10.1371/journal.pone.0044427</doi><oa>free_for_read</oa></addata></record> |
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| recordid | cdi_plos_journals_1326551048 |
| source | MEDLINE; DOAJ Directory of Open Access Journals; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; PubMed Central; Free Full-Text Journals in Chemistry; Public Library of Science (PLoS) |
| subjects | Adenocarcinoma Animal models Animals Biology Cancer therapies Carcinogenesis Carcinogens Computed tomography Correlation Deoxyglucose Dosimeters Dosimetry Drug dosages Feasibility studies Gamma radiation Gamma rays Health physics Histology Humans Image processing Image segmentation Iterative algorithms Iterative methods Laboratory animals Lesions Lung cancer Lung Neoplasms - diagnostic imaging Lung Neoplasms - genetics Lung Neoplasms - metabolism Lung Neoplasms - pathology Lungs Medical imaging Medical schools Medicine Metabolism Mice Mice, Inbred C57BL Mice, Transgenic Morphology Multimodal Imaging Physics Positron emission Positron-Emission Tomography Pressure transducers Proto-Oncogene Proteins c-myc - genetics Proto-Oncogene Proteins c-myc - metabolism Pulmonary Surfactant-Associated Proteins Radiation Radiation dosage raf Kinases - genetics raf Kinases - metabolism Raf protein Regional analysis Rodents Segmentation Thermoluminescence Tomography Tomography, X-Ray Computed Transgenic animals Transgenic mice Tumors Veterinary medicine Veterinary Science γ Radiation |
| title | Combined micro-PET/micro-CT imaging of lung tumours in SPC-raf and SPC-myc transgenic mice |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-02-05T09%3A09%3A32IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_plos_&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Combined%20micro-PET/micro-CT%20imaging%20of%20lung%20tumours%20in%20SPC-raf%20and%20SPC-myc%20transgenic%20mice&rft.jtitle=PloS%20one&rft.au=Rodt,%20Thomas&rft.date=2012-09-21&rft.volume=7&rft.issue=9&rft.spage=e44427&rft.pages=e44427-&rft.issn=1932-6203&rft.eissn=1932-6203&rft_id=info:doi/10.1371/journal.pone.0044427&rft_dat=%3Cproquest_plos_%3E2943665271%3C/proquest_plos_%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=1326551048&rft_id=info:pmid/23028537&rft_doaj_id=oai_doaj_org_article_65974e84be154825b266f2b846bafad6&rfr_iscdi=true |