Standardization of the teratoma assay for analysis of pluripotency of human ES cells and biosafety of their differentiated progeny

Teratoma tumor formation is an essential criterion in determining the pluripotency of human pluripotent stem cells. However, currently there is no consistent protocol for assessment of teratoma forming ability. Here we present detailed characterization of a teratoma assay that is based on subcutaneo...

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Veröffentlicht in:	PloS one 2012-09, Vol.7 (9), p.e45532
Hauptverfasser:	Gropp, Michal, Shilo, Vitali, Vainer, Gilad, Gov, Miri, Gil, Yaniv, Khaner, Hanita, Matzrafi, Limor, Idelson, Maria, Kopolovic, Juri, Zak, Naomi B, Reubinoff, Benjamin E
Format:	Artikel
Sprache:	eng
Schlagworte:	Analysis Animals Assaying Biological Assay - standards Biology Biomarkers - metabolism Biosafety Cell Count Cell cycle Cell Differentiation Collagen - administration & dosage Diagnosis Drug Combinations Embryo cells Embryonic stem cells Embryonic Stem Cells - pathology Embryonic Stem Cells - transplantation Embryos Feeder Cells - cytology Feeder Cells - transplantation Fibroblasts - cytology Fibroblasts - transplantation Forming Gene therapy Gynecology Health aspects Humans Immunodeficiency Injections, Subcutaneous Karyotyping Laminin - administration & dosage Medical research Mice Mice, Inbred NOD Mice, SCID Neurosciences Pathology Physiological aspects Pluripotency Pluripotent Stem Cells - pathology Pluripotent Stem Cells - transplantation Progeny Proteoglycans - administration & dosage Sensitivity and Specificity Standardization Stem cell transplantation Stem cells Survival Rate Teratoma Teratoma - mortality Teratoma - pathology Transplantation Tumors
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