HtrA3 as an early marker for preeclampsia: specific monoclonal antibodies and sensitive high-throughput assays for serum screening
Mammalian HtrA3 (high temperature requirement A3) is a serine protease of the HtrA family. It has two isoforms [long (HtrA3-L) and short (HtrA3-S)] and is important for placental development and cancer progression. Recently, HtrA3 was identified as a potential diagnostic marker for early detection o...
Gespeichert in:
| Veröffentlicht in: | PloS one 2012-09, Vol.7 (9), p.e45956 |
|---|---|
| Hauptverfasser: | , , , , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | |
|---|---|
| container_issue | 9 |
| container_start_page | e45956 |
| container_title | PloS one |
| container_volume | 7 |
| creator | Dynon, Kemperly Heng, Sophea Puryer, Michelle Li, Ying Walton, Kelly Endo, Yaeta Nie, Guiying |
| description | Mammalian HtrA3 (high temperature requirement A3) is a serine protease of the HtrA family. It has two isoforms [long (HtrA3-L) and short (HtrA3-S)] and is important for placental development and cancer progression. Recently, HtrA3 was identified as a potential diagnostic marker for early detection of preeclampsia, a life-threatening pregnancy-specific disorder. Currently there are no high-throughput assays available to detect HtrA3 in human serum. In this study we generated and fully tested a panel of five HtrA3 mouse monoclonal antibodies (mAbs). Three mAbs recognised both HtrA3-L and HtrA3-S and the other two detected HtrA3-L only. All five mAbs were highly specific to HtrA3 and applicable in western blotting and immunohistochemical analysis of endogenous HtrA3 proteins in the mouse and human tissues. Amplified luminescent proximity homogeneous assays-linked immunosorbent assays (AlphaLISAs), were developed to detect HtrA3 isoforms in picomolar levels in serum. The HtrA3 AlphaLISA detected significantly higher serum levels of HtrA3 in women at 13-14 weeks of gestation who subsequently developed preeclampsia compared to gestational-age matched controls. These HtrA3 mAbs are valuable for the development of immunoassays and characterisation of HtrA3 isoform-specific biology. The newly developed HtrA3 AlphaLISA assays are suitable for large scale screening of human serum. |
| doi_str_mv | 10.1371/journal.pone.0045956 |
| format | Article |
| fullrecord | <record><control><sourceid>gale_plos_</sourceid><recordid>TN_cdi_plos_journals_1326547528</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><galeid>A498251783</galeid><doaj_id>oai_doaj_org_article_d3ce7569d8e145918d12198d9d7aa80e</doaj_id><sourcerecordid>A498251783</sourcerecordid><originalsourceid>FETCH-LOGICAL-c758t-24f53a65c9d61f41b424ff91f52454f1b50bd5ac1f485ee036d52349375c865d3</originalsourceid><addsrcrecordid>eNqNkl2L1DAYhYso7of-A9GCIHgxY9IkbeOFMCzqDiws-HUbMsnbNmPb1CRdnNv95WZ2ussUFKQXKW-ec95wOEnyAqMlJgV-t7Wj62W7HGwPS4Qo4yx_lJxiTrJFniHy-Oj_JDnzfosQI2WeP01OMoIo5yg7TW4vg1uRVPpU9ilI1-7STrqf4NLKunRwAKqV3eCNfJ_6AZSpjEo721vV2rg9qoLZWG1gb6BTD703wdxA2pi6WYTG2bFuhjHEDV7u_J2rBzd2qVfRvDd9_Sx5UsnWw_PpPE--f_r47eJycXX9eX2xulqogpVhkdGKEZkzxXWOK4o3NE4qjiuWUUYrvGFoo5lU8a5kAIjkmmWEclIwVeZMk_Pk1cF3aK0XU3xeYJLljBYsKyOxPhDayq0YnIlR7ISVRtwNrKuFdMGoFoQmCgqWc10CjtHjUuMM81JzXUhZIoheH6Zt46YDraAPTrYz0_lNbxpR2xtBKCs4J9Hg9WTg7K8RfPjHkyeqlvFVpq9sNFOd8UqsKC8zhoty77X8CxU_DZ1RsUCVifOZ4O1MEJkAv0MtR-_F-uuX_2evf8zZN0dsA7INjbftGIzt_RykB1A5672D6iE5jMS-__dpiH3_xdT_KHt5nPqD6L7w5A_EngHy</addsrcrecordid><sourcetype>Open Website</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>1326547528</pqid></control><display><type>article</type><title>HtrA3 as an early marker for preeclampsia: specific monoclonal antibodies and sensitive high-throughput assays for serum screening</title><source>MEDLINE</source><source>DOAJ Directory of Open Access Journals</source><source>Public Library of Science (PLoS)</source><source>EZB-FREE-00999 freely available EZB journals</source><source>PubMed Central</source><source>Free Full-Text Journals in Chemistry</source><creator>Dynon, Kemperly ; Heng, Sophea ; Puryer, Michelle ; Li, Ying ; Walton, Kelly ; Endo, Yaeta ; Nie, Guiying</creator><contributor>Wang, Haibin</contributor><creatorcontrib>Dynon, Kemperly ; Heng, Sophea ; Puryer, Michelle ; Li, Ying ; Walton, Kelly ; Endo, Yaeta ; Nie, Guiying ; Wang, Haibin</creatorcontrib><description>Mammalian HtrA3 (high temperature requirement A3) is a serine protease of the HtrA family. It has two isoforms [long (HtrA3-L) and short (HtrA3-S)] and is important for placental development and cancer progression. Recently, HtrA3 was identified as a potential diagnostic marker for early detection of preeclampsia, a life-threatening pregnancy-specific disorder. Currently there are no high-throughput assays available to detect HtrA3 in human serum. In this study we generated and fully tested a panel of five HtrA3 mouse monoclonal antibodies (mAbs). Three mAbs recognised both HtrA3-L and HtrA3-S and the other two detected HtrA3-L only. All five mAbs were highly specific to HtrA3 and applicable in western blotting and immunohistochemical analysis of endogenous HtrA3 proteins in the mouse and human tissues. Amplified luminescent proximity homogeneous assays-linked immunosorbent assays (AlphaLISAs), were developed to detect HtrA3 isoforms in picomolar levels in serum. The HtrA3 AlphaLISA detected significantly higher serum levels of HtrA3 in women at 13-14 weeks of gestation who subsequently developed preeclampsia compared to gestational-age matched controls. These HtrA3 mAbs are valuable for the development of immunoassays and characterisation of HtrA3 isoform-specific biology. The newly developed HtrA3 AlphaLISA assays are suitable for large scale screening of human serum.</description><identifier>ISSN: 1932-6203</identifier><identifier>EISSN: 1932-6203</identifier><identifier>DOI: 10.1371/journal.pone.0045956</identifier><identifier>PMID: 23049902</identifier><language>eng</language><publisher>United States: Public Library of Science</publisher><subject>Adult ; Animals ; Antibodies, Monoclonal - chemistry ; Assaying ; Biological assay ; Biology ; Cancer ; Cardiovascular disease ; Diagnosis ; Diagnostic systems ; DNA methylation ; Endometrium - metabolism ; Epitope Mapping - methods ; Epitopes - chemistry ; Female ; Genetic aspects ; Gestation ; Gestational Age ; Health aspects ; High temperature ; Human tissues ; Humans ; Hypertension ; Immunoassay ; Immunoassay - methods ; Immunoassays ; Innovations ; Insulin ; Isoforms ; Mammals ; Medical research ; Medicine ; Mice ; Mice, Inbred C57BL ; Mice, Knockout ; Monoclonal antibodies ; Ovarian cancer ; Placenta ; Pre-eclampsia ; Pre-Eclampsia - diagnosis ; Pre-Eclampsia - metabolism ; Preeclampsia ; Pregnancy ; Pregnancy Trimester, Second ; Proteases ; Protein Isoforms ; Protein Structure, Tertiary ; Proteins ; Recombinant Proteins - chemistry ; Screening ; Serine ; Serine Endopeptidases - metabolism ; Serine proteinase ; Serum levels ; Stroke ; Systematic review ; Temperature requirements ; Tissue Distribution ; Tissues ; Trophoblasts - cytology ; Uterus - metabolism ; Western blotting ; Womens health</subject><ispartof>PloS one, 2012-09, Vol.7 (9), p.e45956</ispartof><rights>COPYRIGHT 2012 Public Library of Science</rights><rights>Dynon et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License: https://creativecommons.org/licenses/by/4.0/ (the “License”), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><rights>2012 Dynon et al 2012 Dynon et al</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c758t-24f53a65c9d61f41b424ff91f52454f1b50bd5ac1f485ee036d52349375c865d3</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC3457993/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC3457993/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,725,778,782,862,883,2098,2917,23849,27907,27908,53774,53776,79351,79352</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/23049902$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><contributor>Wang, Haibin</contributor><creatorcontrib>Dynon, Kemperly</creatorcontrib><creatorcontrib>Heng, Sophea</creatorcontrib><creatorcontrib>Puryer, Michelle</creatorcontrib><creatorcontrib>Li, Ying</creatorcontrib><creatorcontrib>Walton, Kelly</creatorcontrib><creatorcontrib>Endo, Yaeta</creatorcontrib><creatorcontrib>Nie, Guiying</creatorcontrib><title>HtrA3 as an early marker for preeclampsia: specific monoclonal antibodies and sensitive high-throughput assays for serum screening</title><title>PloS one</title><addtitle>PLoS One</addtitle><description>Mammalian HtrA3 (high temperature requirement A3) is a serine protease of the HtrA family. It has two isoforms [long (HtrA3-L) and short (HtrA3-S)] and is important for placental development and cancer progression. Recently, HtrA3 was identified as a potential diagnostic marker for early detection of preeclampsia, a life-threatening pregnancy-specific disorder. Currently there are no high-throughput assays available to detect HtrA3 in human serum. In this study we generated and fully tested a panel of five HtrA3 mouse monoclonal antibodies (mAbs). Three mAbs recognised both HtrA3-L and HtrA3-S and the other two detected HtrA3-L only. All five mAbs were highly specific to HtrA3 and applicable in western blotting and immunohistochemical analysis of endogenous HtrA3 proteins in the mouse and human tissues. Amplified luminescent proximity homogeneous assays-linked immunosorbent assays (AlphaLISAs), were developed to detect HtrA3 isoforms in picomolar levels in serum. The HtrA3 AlphaLISA detected significantly higher serum levels of HtrA3 in women at 13-14 weeks of gestation who subsequently developed preeclampsia compared to gestational-age matched controls. These HtrA3 mAbs are valuable for the development of immunoassays and characterisation of HtrA3 isoform-specific biology. The newly developed HtrA3 AlphaLISA assays are suitable for large scale screening of human serum.</description><subject>Adult</subject><subject>Animals</subject><subject>Antibodies, Monoclonal - chemistry</subject><subject>Assaying</subject><subject>Biological assay</subject><subject>Biology</subject><subject>Cancer</subject><subject>Cardiovascular disease</subject><subject>Diagnosis</subject><subject>Diagnostic systems</subject><subject>DNA methylation</subject><subject>Endometrium - metabolism</subject><subject>Epitope Mapping - methods</subject><subject>Epitopes - chemistry</subject><subject>Female</subject><subject>Genetic aspects</subject><subject>Gestation</subject><subject>Gestational Age</subject><subject>Health aspects</subject><subject>High temperature</subject><subject>Human tissues</subject><subject>Humans</subject><subject>Hypertension</subject><subject>Immunoassay</subject><subject>Immunoassay - methods</subject><subject>Immunoassays</subject><subject>Innovations</subject><subject>Insulin</subject><subject>Isoforms</subject><subject>Mammals</subject><subject>Medical research</subject><subject>Medicine</subject><subject>Mice</subject><subject>Mice, Inbred C57BL</subject><subject>Mice, Knockout</subject><subject>Monoclonal antibodies</subject><subject>Ovarian cancer</subject><subject>Placenta</subject><subject>Pre-eclampsia</subject><subject>Pre-Eclampsia - diagnosis</subject><subject>Pre-Eclampsia - metabolism</subject><subject>Preeclampsia</subject><subject>Pregnancy</subject><subject>Pregnancy Trimester, Second</subject><subject>Proteases</subject><subject>Protein Isoforms</subject><subject>Protein Structure, Tertiary</subject><subject>Proteins</subject><subject>Recombinant Proteins - chemistry</subject><subject>Screening</subject><subject>Serine</subject><subject>Serine Endopeptidases - metabolism</subject><subject>Serine proteinase</subject><subject>Serum levels</subject><subject>Stroke</subject><subject>Systematic review</subject><subject>Temperature requirements</subject><subject>Tissue Distribution</subject><subject>Tissues</subject><subject>Trophoblasts - cytology</subject><subject>Uterus - metabolism</subject><subject>Western blotting</subject><subject>Womens health</subject><issn>1932-6203</issn><issn>1932-6203</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2012</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><sourceid>DOA</sourceid><recordid>eNqNkl2L1DAYhYso7of-A9GCIHgxY9IkbeOFMCzqDiws-HUbMsnbNmPb1CRdnNv95WZ2ussUFKQXKW-ec95wOEnyAqMlJgV-t7Wj62W7HGwPS4Qo4yx_lJxiTrJFniHy-Oj_JDnzfosQI2WeP01OMoIo5yg7TW4vg1uRVPpU9ilI1-7STrqf4NLKunRwAKqV3eCNfJ_6AZSpjEo721vV2rg9qoLZWG1gb6BTD703wdxA2pi6WYTG2bFuhjHEDV7u_J2rBzd2qVfRvDd9_Sx5UsnWw_PpPE--f_r47eJycXX9eX2xulqogpVhkdGKEZkzxXWOK4o3NE4qjiuWUUYrvGFoo5lU8a5kAIjkmmWEclIwVeZMk_Pk1cF3aK0XU3xeYJLljBYsKyOxPhDayq0YnIlR7ISVRtwNrKuFdMGoFoQmCgqWc10CjtHjUuMM81JzXUhZIoheH6Zt46YDraAPTrYz0_lNbxpR2xtBKCs4J9Hg9WTg7K8RfPjHkyeqlvFVpq9sNFOd8UqsKC8zhoty77X8CxU_DZ1RsUCVifOZ4O1MEJkAv0MtR-_F-uuX_2evf8zZN0dsA7INjbftGIzt_RykB1A5672D6iE5jMS-__dpiH3_xdT_KHt5nPqD6L7w5A_EngHy</recordid><startdate>20120925</startdate><enddate>20120925</enddate><creator>Dynon, Kemperly</creator><creator>Heng, Sophea</creator><creator>Puryer, Michelle</creator><creator>Li, Ying</creator><creator>Walton, Kelly</creator><creator>Endo, Yaeta</creator><creator>Nie, Guiying</creator><general>Public Library of Science</general><general>Public Library of Science (PLoS)</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>IOV</scope><scope>ISR</scope><scope>3V.</scope><scope>7QG</scope><scope>7QL</scope><scope>7QO</scope><scope>7RV</scope><scope>7SN</scope><scope>7SS</scope><scope>7T5</scope><scope>7TG</scope><scope>7TM</scope><scope>7U9</scope><scope>7X2</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>8AO</scope><scope>8C1</scope><scope>8FD</scope><scope>8FE</scope><scope>8FG</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABJCF</scope><scope>ABUWG</scope><scope>AEUYN</scope><scope>AFKRA</scope><scope>ARAPS</scope><scope>ATCPS</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BGLVJ</scope><scope>BHPHI</scope><scope>C1K</scope><scope>CCPQU</scope><scope>D1I</scope><scope>DWQXO</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>H94</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>KB.</scope><scope>KB0</scope><scope>KL.</scope><scope>L6V</scope><scope>LK8</scope><scope>M0K</scope><scope>M0S</scope><scope>M1P</scope><scope>M7N</scope><scope>M7P</scope><scope>M7S</scope><scope>NAPCQ</scope><scope>P5Z</scope><scope>P62</scope><scope>P64</scope><scope>PATMY</scope><scope>PDBOC</scope><scope>PIMPY</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>PTHSS</scope><scope>PYCSY</scope><scope>RC3</scope><scope>5PM</scope><scope>DOA</scope></search><sort><creationdate>20120925</creationdate><title>HtrA3 as an early marker for preeclampsia: specific monoclonal antibodies and sensitive high-throughput assays for serum screening</title><author>Dynon, Kemperly ; Heng, Sophea ; Puryer, Michelle ; Li, Ying ; Walton, Kelly ; Endo, Yaeta ; Nie, Guiying</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c758t-24f53a65c9d61f41b424ff91f52454f1b50bd5ac1f485ee036d52349375c865d3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2012</creationdate><topic>Adult</topic><topic>Animals</topic><topic>Antibodies, Monoclonal - chemistry</topic><topic>Assaying</topic><topic>Biological assay</topic><topic>Biology</topic><topic>Cancer</topic><topic>Cardiovascular disease</topic><topic>Diagnosis</topic><topic>Diagnostic systems</topic><topic>DNA methylation</topic><topic>Endometrium - metabolism</topic><topic>Epitope Mapping - methods</topic><topic>Epitopes - chemistry</topic><topic>Female</topic><topic>Genetic aspects</topic><topic>Gestation</topic><topic>Gestational Age</topic><topic>Health aspects</topic><topic>High temperature</topic><topic>Human tissues</topic><topic>Humans</topic><topic>Hypertension</topic><topic>Immunoassay</topic><topic>Immunoassay - methods</topic><topic>Immunoassays</topic><topic>Innovations</topic><topic>Insulin</topic><topic>Isoforms</topic><topic>Mammals</topic><topic>Medical research</topic><topic>Medicine</topic><topic>Mice</topic><topic>Mice, Inbred C57BL</topic><topic>Mice, Knockout</topic><topic>Monoclonal antibodies</topic><topic>Ovarian cancer</topic><topic>Placenta</topic><topic>Pre-eclampsia</topic><topic>Pre-Eclampsia - diagnosis</topic><topic>Pre-Eclampsia - metabolism</topic><topic>Preeclampsia</topic><topic>Pregnancy</topic><topic>Pregnancy Trimester, Second</topic><topic>Proteases</topic><topic>Protein Isoforms</topic><topic>Protein Structure, Tertiary</topic><topic>Proteins</topic><topic>Recombinant Proteins - chemistry</topic><topic>Screening</topic><topic>Serine</topic><topic>Serine Endopeptidases - metabolism</topic><topic>Serine proteinase</topic><topic>Serum levels</topic><topic>Stroke</topic><topic>Systematic review</topic><topic>Temperature requirements</topic><topic>Tissue Distribution</topic><topic>Tissues</topic><topic>Trophoblasts - cytology</topic><topic>Uterus - metabolism</topic><topic>Western blotting</topic><topic>Womens health</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Dynon, Kemperly</creatorcontrib><creatorcontrib>Heng, Sophea</creatorcontrib><creatorcontrib>Puryer, Michelle</creatorcontrib><creatorcontrib>Li, Ying</creatorcontrib><creatorcontrib>Walton, Kelly</creatorcontrib><creatorcontrib>Endo, Yaeta</creatorcontrib><creatorcontrib>Nie, Guiying</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Gale In Context: Opposing Viewpoints</collection><collection>Gale In Context: Science</collection><collection>ProQuest Central (Corporate)</collection><collection>Animal Behavior Abstracts</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Biotechnology Research Abstracts</collection><collection>Nursing & Allied Health Database</collection><collection>Ecology Abstracts</collection><collection>Entomology Abstracts (Full archive)</collection><collection>Immunology Abstracts</collection><collection>Meteorological & Geoastrophysical Abstracts</collection><collection>Nucleic Acids Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>Agricultural Science Collection</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Medical Database (Alumni Edition)</collection><collection>ProQuest Pharma Collection</collection><collection>Public Health Database</collection><collection>Technology Research Database</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Technology Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>Materials Science & Engineering Collection</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest One Sustainability</collection><collection>ProQuest Central UK/Ireland</collection><collection>Advanced Technologies & Aerospace Collection</collection><collection>Agricultural & Environmental Science Collection</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Technology Collection</collection><collection>Natural Science Collection</collection><collection>Environmental Sciences and Pollution Management</collection><collection>ProQuest One Community College</collection><collection>ProQuest Materials Science Collection</collection><collection>ProQuest Central Korea</collection><collection>Engineering Research Database</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Materials Science Database</collection><collection>Nursing & Allied Health Database (Alumni Edition)</collection><collection>Meteorological & Geoastrophysical Abstracts - Academic</collection><collection>ProQuest Engineering Collection</collection><collection>ProQuest Biological Science Collection</collection><collection>Agricultural Science Database</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biological Science Database</collection><collection>Engineering Database</collection><collection>Nursing & Allied Health Premium</collection><collection>Advanced Technologies & Aerospace Database</collection><collection>ProQuest Advanced Technologies & Aerospace Collection</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Environmental Science Database</collection><collection>Materials Science Collection</collection><collection>Publicly Available Content Database</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><collection>Engineering Collection</collection><collection>Environmental Science Collection</collection><collection>Genetics Abstracts</collection><collection>PubMed Central (Full Participant titles)</collection><collection>DOAJ Directory of Open Access Journals</collection><jtitle>PloS one</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Dynon, Kemperly</au><au>Heng, Sophea</au><au>Puryer, Michelle</au><au>Li, Ying</au><au>Walton, Kelly</au><au>Endo, Yaeta</au><au>Nie, Guiying</au><au>Wang, Haibin</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>HtrA3 as an early marker for preeclampsia: specific monoclonal antibodies and sensitive high-throughput assays for serum screening</atitle><jtitle>PloS one</jtitle><addtitle>PLoS One</addtitle><date>2012-09-25</date><risdate>2012</risdate><volume>7</volume><issue>9</issue><spage>e45956</spage><pages>e45956-</pages><issn>1932-6203</issn><eissn>1932-6203</eissn><abstract>Mammalian HtrA3 (high temperature requirement A3) is a serine protease of the HtrA family. It has two isoforms [long (HtrA3-L) and short (HtrA3-S)] and is important for placental development and cancer progression. Recently, HtrA3 was identified as a potential diagnostic marker for early detection of preeclampsia, a life-threatening pregnancy-specific disorder. Currently there are no high-throughput assays available to detect HtrA3 in human serum. In this study we generated and fully tested a panel of five HtrA3 mouse monoclonal antibodies (mAbs). Three mAbs recognised both HtrA3-L and HtrA3-S and the other two detected HtrA3-L only. All five mAbs were highly specific to HtrA3 and applicable in western blotting and immunohistochemical analysis of endogenous HtrA3 proteins in the mouse and human tissues. Amplified luminescent proximity homogeneous assays-linked immunosorbent assays (AlphaLISAs), were developed to detect HtrA3 isoforms in picomolar levels in serum. The HtrA3 AlphaLISA detected significantly higher serum levels of HtrA3 in women at 13-14 weeks of gestation who subsequently developed preeclampsia compared to gestational-age matched controls. These HtrA3 mAbs are valuable for the development of immunoassays and characterisation of HtrA3 isoform-specific biology. The newly developed HtrA3 AlphaLISA assays are suitable for large scale screening of human serum.</abstract><cop>United States</cop><pub>Public Library of Science</pub><pmid>23049902</pmid><doi>10.1371/journal.pone.0045956</doi><tpages>e45956</tpages><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 1932-6203 |
| ispartof | PloS one, 2012-09, Vol.7 (9), p.e45956 |
| issn | 1932-6203 1932-6203 |
| language | eng |
| recordid | cdi_plos_journals_1326547528 |
| source | MEDLINE; DOAJ Directory of Open Access Journals; Public Library of Science (PLoS); EZB-FREE-00999 freely available EZB journals; PubMed Central; Free Full-Text Journals in Chemistry |
| subjects | Adult Animals Antibodies, Monoclonal - chemistry Assaying Biological assay Biology Cancer Cardiovascular disease Diagnosis Diagnostic systems DNA methylation Endometrium - metabolism Epitope Mapping - methods Epitopes - chemistry Female Genetic aspects Gestation Gestational Age Health aspects High temperature Human tissues Humans Hypertension Immunoassay Immunoassay - methods Immunoassays Innovations Insulin Isoforms Mammals Medical research Medicine Mice Mice, Inbred C57BL Mice, Knockout Monoclonal antibodies Ovarian cancer Placenta Pre-eclampsia Pre-Eclampsia - diagnosis Pre-Eclampsia - metabolism Preeclampsia Pregnancy Pregnancy Trimester, Second Proteases Protein Isoforms Protein Structure, Tertiary Proteins Recombinant Proteins - chemistry Screening Serine Serine Endopeptidases - metabolism Serine proteinase Serum levels Stroke Systematic review Temperature requirements Tissue Distribution Tissues Trophoblasts - cytology Uterus - metabolism Western blotting Womens health |
| title | HtrA3 as an early marker for preeclampsia: specific monoclonal antibodies and sensitive high-throughput assays for serum screening |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-16T22%3A25%3A13IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-gale_plos_&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=HtrA3%20as%20an%20early%20marker%20for%20preeclampsia:%20specific%20monoclonal%20antibodies%20and%20sensitive%20high-throughput%20assays%20for%20serum%20screening&rft.jtitle=PloS%20one&rft.au=Dynon,%20Kemperly&rft.date=2012-09-25&rft.volume=7&rft.issue=9&rft.spage=e45956&rft.pages=e45956-&rft.issn=1932-6203&rft.eissn=1932-6203&rft_id=info:doi/10.1371/journal.pone.0045956&rft_dat=%3Cgale_plos_%3EA498251783%3C/gale_plos_%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=1326547528&rft_id=info:pmid/23049902&rft_galeid=A498251783&rft_doaj_id=oai_doaj_org_article_d3ce7569d8e145918d12198d9d7aa80e&rfr_iscdi=true |