A versatile overexpression strategy in the pathogenic yeast Candida albicans: identification of regulators of morphogenesis and fitness
Candida albicans is the most frequently encountered human fungal pathogen, causing both superficial infections and life-threatening systemic diseases. Functional genomic studies performed in this organism have mainly used knock-out mutants and extensive collections of overexpression mutants are stil...
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| Veröffentlicht in: | PloS one 2012-09, Vol.7 (9), p.e45912-e45912 |
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| creator | Chauvel, Murielle Nesseir, Audrey Cabral, Vitor Znaidi, Sadri Goyard, Sophie Bachellier-Bassi, Sophie Firon, Arnaud Legrand, Mélanie Diogo, Dorothée Naulleau, Claire Rossignol, Tristan d'Enfert, Christophe |
| description | Candida albicans is the most frequently encountered human fungal pathogen, causing both superficial infections and life-threatening systemic diseases. Functional genomic studies performed in this organism have mainly used knock-out mutants and extensive collections of overexpression mutants are still lacking. Here, we report the development of a first generation C. albicans ORFeome, the improvement of overexpression systems and the construction of two new libraries of C. albicans strains overexpressing genes for components of signaling networks, in particular protein kinases, protein phosphatases and transcription factors. As a proof of concept, we screened these collections for genes whose overexpression impacts morphogenesis or growth rates in C. albicans. Our screens identified genes previously described for their role in these biological processes, demonstrating the functionality of our strategy, as well as genes that have not been previously associated to these processes. This article emphasizes the potential of systematic overexpression strategies to improve our knowledge of regulatory networks in C. albicans. The C. albicans plasmid and strain collections described here are available at the Fungal Genetics Stock Center. Their extension to a genome-wide scale will represent important resources for the C. albicans community. |
| doi_str_mv | 10.1371/journal.pone.0045912 |
| format | Article |
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Functional genomic studies performed in this organism have mainly used knock-out mutants and extensive collections of overexpression mutants are still lacking. Here, we report the development of a first generation C. albicans ORFeome, the improvement of overexpression systems and the construction of two new libraries of C. albicans strains overexpressing genes for components of signaling networks, in particular protein kinases, protein phosphatases and transcription factors. As a proof of concept, we screened these collections for genes whose overexpression impacts morphogenesis or growth rates in C. albicans. Our screens identified genes previously described for their role in these biological processes, demonstrating the functionality of our strategy, as well as genes that have not been previously associated to these processes. This article emphasizes the potential of systematic overexpression strategies to improve our knowledge of regulatory networks in C. albicans. The C. albicans plasmid and strain collections described here are available at the Fungal Genetics Stock Center. 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This is an open-access article distributed under the terms of the Creative Commons Attribution License: https://creativecommons.org/licenses/by/4.0/ (the “License”), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. 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| recordid | cdi_plos_journals_1326547513 |
| source | MEDLINE; Public Library of Science (PLoS) Journals Open Access; DOAJ Directory of Open Access Journals; EZB-FREE-00999 freely available EZB journals; PubMed Central; Free Full-Text Journals in Chemistry |
| subjects | Baking yeast Biofilms Biological activity Biology Candida Candida albicans Candida albicans - metabolism Doxycycline Doxycycline - pharmacology E coli Fitness Fungal Proteins Fungal Proteins - metabolism Fungi Gene expression Gene Expression Regulation, Fungal Gene Library Genes Genes, Fungal Genetic aspects Genetics Genome, Fungal Genomes Genomics Green Fluorescent Proteins Green Fluorescent Proteins - metabolism Hypothesis testing Image Processing, Computer-Assisted Kinases Kinetics Knowledge management Life Sciences Microbiology and Parasitology Models, Genetic Morphogenesis Mutants Mycology Open Reading Frames Physiological aspects Polymerase Chain Reaction Polymerase Chain Reaction - methods Promoter Regions, Genetic Protein kinase Proteins Regulators Reproductive fitness Saccharomyces cerevisiae Screens Signal Transduction Signaling Studies Tetracycline Tetracycline - pharmacology Transcription factors Yeast |
| title | A versatile overexpression strategy in the pathogenic yeast Candida albicans: identification of regulators of morphogenesis and fitness |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2024-12-28T04%3A41%3A12IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-gale_plos_&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=A%20versatile%20overexpression%20strategy%20in%20the%20pathogenic%20yeast%20Candida%20albicans:%20identification%20of%20regulators%20of%20morphogenesis%20and%20fitness&rft.jtitle=PloS%20one&rft.au=Chauvel,%20Murielle&rft.date=2012-09-25&rft.volume=7&rft.issue=9&rft.spage=e45912&rft.epage=e45912&rft.pages=e45912-e45912&rft.issn=1932-6203&rft.eissn=1932-6203&rft_id=info:doi/10.1371/journal.pone.0045912&rft_dat=%3Cgale_plos_%3EA498251710%3C/gale_plos_%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=1326547513&rft_id=info:pmid/23049891&rft_galeid=A498251710&rft_doaj_id=oai_doaj_org_article_66700dc5d0144f9b9f516db41f8c8b36&rfr_iscdi=true |