Beta cell count instead of beta cell mass to assess and localize growth in beta cell population following pancreatic duct ligation in mice
Pancreatic-tail duct ligation (PDL) in adult rodents has been reported to induce beta cell generation and increase beta cell mass but increases in beta cell number have not been demonstrated. This study examines whether PDL increases beta cell number and whether this is caused by neogenesis of small...
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| Veröffentlicht in: | PloS one 2012-08, Vol.7 (8), p.e43959-e43959 |
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| creator | Chintinne, Marie Stangé, Geert Denys, Bart Ling, Zhidong In 't Veld, Peter Pipeleers, Daniel |
| description | Pancreatic-tail duct ligation (PDL) in adult rodents has been reported to induce beta cell generation and increase beta cell mass but increases in beta cell number have not been demonstrated. This study examines whether PDL increases beta cell number and whether this is caused by neogenesis of small clusters and/or their growth to larger aggregates.
Total beta cell number and its distribution over small (100 µm) clusters was determined in pancreatic tails of 10-week-old mice, 2 weeks after PDL or sham.
PDL increased total beta cell mass but not total beta cell number. It induced neogenesis of small beta cell clusters (2.2-fold higher number) which contained a higher percent proliferating beta cells (1.9% Ki67+cells) than sham tails ( |
| doi_str_mv | 10.1371/journal.pone.0043959 |
| format | Article |
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Total beta cell number and its distribution over small (<50 µm), medium, large (>100 µm) clusters was determined in pancreatic tails of 10-week-old mice, 2 weeks after PDL or sham.
PDL increased total beta cell mass but not total beta cell number. It induced neogenesis of small beta cell clusters (2.2-fold higher number) which contained a higher percent proliferating beta cells (1.9% Ki67+cells) than sham tails (<0.2%); their higher beta cell number represented <5% of total beta cell number and was associated with a similar increase in alpha cell number. It is unknown whether the regenerative process is causally related to the inflammatory infiltration in PDL-tails. Human pancreases with inflammatory infiltration also exhibited activation of proliferation in small beta cell clusters.
The PDL model illustrates the advantage of direct beta cell counts over beta cell mass measurements when assessing and localizing beta cell regeneration in the pancreas. It demonstrates the ability of the adult mouse pancreas for neogenesis of small beta cell clusters with activated beta cell proliferation. Further studies should investigate conditions under which neoformed small beta cell clusters grow to larger aggregates and hence to higher total beta cell numbers.</description><identifier>ISSN: 1932-6203</identifier><identifier>EISSN: 1932-6203</identifier><identifier>DOI: 10.1371/journal.pone.0043959</identifier><identifier>PMID: 22952825</identifier><language>eng</language><publisher>United States: Public Library of Science</publisher><subject>Aggregates ; Animals ; Beta cells ; Biology ; Cell Count ; Cell number ; Cell Proliferation ; Cell Size ; Clusters ; Diabetes ; Glucose ; Humans ; Infiltration ; Inflammation ; Insulin ; Insulin-Secreting Cells - cytology ; Laboratories ; Ligation ; Male ; Medicine ; Mice ; Pancreas ; Pancreatic Ducts - cytology ; Pancreatic Ducts - surgery ; Regeneration ; Rodents ; Tails</subject><ispartof>PloS one, 2012-08, Vol.7 (8), p.e43959-e43959</ispartof><rights>Chintinne et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License: https://creativecommons.org/licenses/by/4.0/ (the “License”), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><rights>2012 Chintinne et al 2012 Chintinne et al</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c592t-37ed52e03434a0dec8031a174c27838ac1c21cbbff6b18ca7dbfeaa62d32f1f03</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC3431350/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC3431350/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,727,780,784,864,885,2102,2928,23866,27924,27925,53791,53793,79600,79601</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/22952825$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><contributor>Lynn, Francis C.</contributor><creatorcontrib>Chintinne, Marie</creatorcontrib><creatorcontrib>Stangé, Geert</creatorcontrib><creatorcontrib>Denys, Bart</creatorcontrib><creatorcontrib>Ling, Zhidong</creatorcontrib><creatorcontrib>In 't Veld, Peter</creatorcontrib><creatorcontrib>Pipeleers, Daniel</creatorcontrib><title>Beta cell count instead of beta cell mass to assess and localize growth in beta cell population following pancreatic duct ligation in mice</title><title>PloS one</title><addtitle>PLoS One</addtitle><description>Pancreatic-tail duct ligation (PDL) in adult rodents has been reported to induce beta cell generation and increase beta cell mass but increases in beta cell number have not been demonstrated. This study examines whether PDL increases beta cell number and whether this is caused by neogenesis of small clusters and/or their growth to larger aggregates.
Total beta cell number and its distribution over small (<50 µm), medium, large (>100 µm) clusters was determined in pancreatic tails of 10-week-old mice, 2 weeks after PDL or sham.
PDL increased total beta cell mass but not total beta cell number. It induced neogenesis of small beta cell clusters (2.2-fold higher number) which contained a higher percent proliferating beta cells (1.9% Ki67+cells) than sham tails (<0.2%); their higher beta cell number represented <5% of total beta cell number and was associated with a similar increase in alpha cell number. It is unknown whether the regenerative process is causally related to the inflammatory infiltration in PDL-tails. Human pancreases with inflammatory infiltration also exhibited activation of proliferation in small beta cell clusters.
The PDL model illustrates the advantage of direct beta cell counts over beta cell mass measurements when assessing and localizing beta cell regeneration in the pancreas. It demonstrates the ability of the adult mouse pancreas for neogenesis of small beta cell clusters with activated beta cell proliferation. Further studies should investigate conditions under which neoformed small beta cell clusters grow to larger aggregates and hence to higher total beta cell numbers.</description><subject>Aggregates</subject><subject>Animals</subject><subject>Beta cells</subject><subject>Biology</subject><subject>Cell Count</subject><subject>Cell number</subject><subject>Cell Proliferation</subject><subject>Cell Size</subject><subject>Clusters</subject><subject>Diabetes</subject><subject>Glucose</subject><subject>Humans</subject><subject>Infiltration</subject><subject>Inflammation</subject><subject>Insulin</subject><subject>Insulin-Secreting Cells - cytology</subject><subject>Laboratories</subject><subject>Ligation</subject><subject>Male</subject><subject>Medicine</subject><subject>Mice</subject><subject>Pancreas</subject><subject>Pancreatic Ducts - cytology</subject><subject>Pancreatic Ducts - surgery</subject><subject>Regeneration</subject><subject>Rodents</subject><subject>Tails</subject><issn>1932-6203</issn><issn>1932-6203</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2012</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><sourceid>DOA</sourceid><recordid>eNptUsuO1DAQjBCIXRb-AIElLlxm8DOJL0iw4rHSSlzgbHWc9mxGHjvYCSv4BL4aD5MdzSJO3XJXlbtaVVXPGV0z0bA32zinAH49xoBrSqXQSj-ozpkWfFVzKh6e9GfVk5y3lCrR1vXj6oxzrXjL1Xn1-z1OQCx6T2ycw0SGkCeEnkRHuuNoBzmTKZJSsHQQeuKjBT_8QrJJ8Xa6KbwT_BjH2cM0xEBc9D7eDmFDRgg2YXm1pJ_tRPywOUAKdTdYfFo9cuAzPlvqRfXt44evl59X118-XV2-u15Zpfm0Eg32iiMVUkigPdqWCgaskZY3rWjBMsuZ7Trn6o61Fpq-cwhQ815wxxwVF9XLg-7oYzbLGbNhgtdKCiV1QVwdEH2ErRnTsIP000QYzN-HmDYGUvHh0TjJG-g77RqnZI2otUJhUaNyvZYaitbb5be522FvMUwJ_D3R-5Mw3JhN_GGKPybUft3Xi0CK32fMk9kNeX9lCBjnsjcVbTlMK2WBvvoH-n938oCyKeac0B2XYdTso3XHMvtomSVahfbi1MiRdJcl8QdwxdA9</recordid><startdate>20120830</startdate><enddate>20120830</enddate><creator>Chintinne, Marie</creator><creator>Stangé, Geert</creator><creator>Denys, Bart</creator><creator>Ling, Zhidong</creator><creator>In 't Veld, Peter</creator><creator>Pipeleers, Daniel</creator><general>Public Library of Science</general><general>Public Library of Science (PLoS)</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7QG</scope><scope>7QL</scope><scope>7QO</scope><scope>7RV</scope><scope>7SN</scope><scope>7SS</scope><scope>7T5</scope><scope>7TG</scope><scope>7TM</scope><scope>7U9</scope><scope>7X2</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>8AO</scope><scope>8C1</scope><scope>8FD</scope><scope>8FE</scope><scope>8FG</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABJCF</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>ARAPS</scope><scope>ATCPS</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BGLVJ</scope><scope>BHPHI</scope><scope>C1K</scope><scope>CCPQU</scope><scope>D1I</scope><scope>DWQXO</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>H94</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>KB.</scope><scope>KB0</scope><scope>KL.</scope><scope>L6V</scope><scope>LK8</scope><scope>M0K</scope><scope>M0S</scope><scope>M1P</scope><scope>M7N</scope><scope>M7P</scope><scope>M7S</scope><scope>NAPCQ</scope><scope>P5Z</scope><scope>P62</scope><scope>P64</scope><scope>PATMY</scope><scope>PDBOC</scope><scope>PIMPY</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PTHSS</scope><scope>PYCSY</scope><scope>RC3</scope><scope>7X8</scope><scope>5PM</scope><scope>DOA</scope></search><sort><creationdate>20120830</creationdate><title>Beta cell count instead of beta cell mass to assess and localize growth in beta cell population following pancreatic duct ligation in mice</title><author>Chintinne, Marie ; Stangé, Geert ; Denys, Bart ; Ling, Zhidong ; In 't Veld, Peter ; Pipeleers, Daniel</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c592t-37ed52e03434a0dec8031a174c27838ac1c21cbbff6b18ca7dbfeaa62d32f1f03</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2012</creationdate><topic>Aggregates</topic><topic>Animals</topic><topic>Beta cells</topic><topic>Biology</topic><topic>Cell Count</topic><topic>Cell number</topic><topic>Cell Proliferation</topic><topic>Cell Size</topic><topic>Clusters</topic><topic>Diabetes</topic><topic>Glucose</topic><topic>Humans</topic><topic>Infiltration</topic><topic>Inflammation</topic><topic>Insulin</topic><topic>Insulin-Secreting Cells - 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Academic</collection><collection>PubMed Central (Full Participant titles)</collection><collection>DOAJ Directory of Open Access Journals</collection><jtitle>PloS one</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Chintinne, Marie</au><au>Stangé, Geert</au><au>Denys, Bart</au><au>Ling, Zhidong</au><au>In 't Veld, Peter</au><au>Pipeleers, Daniel</au><au>Lynn, Francis C.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Beta cell count instead of beta cell mass to assess and localize growth in beta cell population following pancreatic duct ligation in mice</atitle><jtitle>PloS one</jtitle><addtitle>PLoS One</addtitle><date>2012-08-30</date><risdate>2012</risdate><volume>7</volume><issue>8</issue><spage>e43959</spage><epage>e43959</epage><pages>e43959-e43959</pages><issn>1932-6203</issn><eissn>1932-6203</eissn><abstract>Pancreatic-tail duct ligation (PDL) in adult rodents has been reported to induce beta cell generation and increase beta cell mass but increases in beta cell number have not been demonstrated. This study examines whether PDL increases beta cell number and whether this is caused by neogenesis of small clusters and/or their growth to larger aggregates.
Total beta cell number and its distribution over small (<50 µm), medium, large (>100 µm) clusters was determined in pancreatic tails of 10-week-old mice, 2 weeks after PDL or sham.
PDL increased total beta cell mass but not total beta cell number. It induced neogenesis of small beta cell clusters (2.2-fold higher number) which contained a higher percent proliferating beta cells (1.9% Ki67+cells) than sham tails (<0.2%); their higher beta cell number represented <5% of total beta cell number and was associated with a similar increase in alpha cell number. It is unknown whether the regenerative process is causally related to the inflammatory infiltration in PDL-tails. Human pancreases with inflammatory infiltration also exhibited activation of proliferation in small beta cell clusters.
The PDL model illustrates the advantage of direct beta cell counts over beta cell mass measurements when assessing and localizing beta cell regeneration in the pancreas. It demonstrates the ability of the adult mouse pancreas for neogenesis of small beta cell clusters with activated beta cell proliferation. Further studies should investigate conditions under which neoformed small beta cell clusters grow to larger aggregates and hence to higher total beta cell numbers.</abstract><cop>United States</cop><pub>Public Library of Science</pub><pmid>22952825</pmid><doi>10.1371/journal.pone.0043959</doi><oa>free_for_read</oa></addata></record> |
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| subjects | Aggregates Animals Beta cells Biology Cell Count Cell number Cell Proliferation Cell Size Clusters Diabetes Glucose Humans Infiltration Inflammation Insulin Insulin-Secreting Cells - cytology Laboratories Ligation Male Medicine Mice Pancreas Pancreatic Ducts - cytology Pancreatic Ducts - surgery Regeneration Rodents Tails |
| title | Beta cell count instead of beta cell mass to assess and localize growth in beta cell population following pancreatic duct ligation in mice |
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