Tonoplast- and plasma membrane-localized aquaporin-family transporters in blue hydrangea sepals of aluminum hyperaccumulating plant
Hydrangea (Hydrangea macrophylla) is tolerant of acidic soils in which toxicity generally arises from the presence of the soluble aluminum (Al) ion. When hydrangea is cultivated in acidic soil, its resulting blue sepal color is caused by the Al complex formation of anthocyanin. The concentration of...
Gespeichert in:
| Veröffentlicht in: | PloS one 2012-08, Vol.7 (8), p.e43189 |
|---|---|
| Hauptverfasser: | , , , , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | |
|---|---|
| container_issue | 8 |
| container_start_page | e43189 |
| container_title | PloS one |
| container_volume | 7 |
| creator | Negishi, Takashi Oshima, Kenshiro Hattori, Masahira Kanai, Masatake Mano, Shoji Nishimura, Mikio Yoshida, Kumi |
| description | Hydrangea (Hydrangea macrophylla) is tolerant of acidic soils in which toxicity generally arises from the presence of the soluble aluminum (Al) ion. When hydrangea is cultivated in acidic soil, its resulting blue sepal color is caused by the Al complex formation of anthocyanin. The concentration of vacuolar Al in blue sepal cells can reach levels in excess of approximately 15 mM, suggesting the existence of an Al-transport and/or storage system. However, until now, no Al transporter has been identified in Al hyperaccumulating plants, animals or microorganisms. To identify the transporter being responsible for Al hyperaccumulation, we prepared a cDNA library from blue sepals according to the sepal maturation stage, and then selected candidate genes using a microarray analysis and an in silico study. Here, we identified the vacuolar and plasma membrane-localized Al transporters genes vacuolar Al transporter (VALT) and plasma membrane Al transporter 1 (PALT1), respectively, which are both members of the aquaporin family. The localization of each protein was confirmed by the transient co-expression of the genes. Reverse transcription-PCR and immunoblotting results indicated that VALT and PALT1 are highly expressed in sepal tissue. The overexpression of VALT and PALT1 in Arabidopsis thaliana conferred Al-tolerance and Al-sensitivity, respectively. |
| doi_str_mv | 10.1371/journal.pone.0043189 |
| format | Article |
| fullrecord | <record><control><sourceid>gale_plos_</sourceid><recordid>TN_cdi_plos_journals_1326450621</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><galeid>A498244339</galeid><doaj_id>oai_doaj_org_article_f1fbc6b6b00f42e6a271d647c1867881</doaj_id><sourcerecordid>A498244339</sourcerecordid><originalsourceid>FETCH-LOGICAL-c758t-3a03bd0360a9c76bf175d270a8f1bfa58e7cedd3a3a01de7affa9c04bd7f3c753</originalsourceid><addsrcrecordid>eNqNk12L1DAUhoso7rr6D0QLguBFx6Rp086NsCx-DCws6OptOM1HJ0uadJNUHG_942ac7jIFBclFwjnPec_hJSfLnmO0wqTBb2_c5C2Y1eisXCFUEdyuH2SneE3KgpaIPDx6n2RPQrhBqCYtpY-zk7Jc1yWtqtPs17WzbjQQYpGDFfn-OUA-yKHzYGVhHAejf0qRw-0Eo_PaFgoGbXZ5TEBIkSh9yLXNOzPJfLsTKdxLyIMcwYTcqRzMNGg7DSk5Sg-cT8NkIGrb79vZ-DR7pBIqn833Wfb1w_vri0_F5dXHzcX5ZcGbuo0FAUQ6gQhFsOYN7RRualE2CFqFOwV1KxsuhSCQQCxkA0olEFWdaBRJEuQse3nQHY0LbPYvMEySFTWiJU7E5kAIBzds9HoAv2MONPsTcL5n4KPmRjKFVcdpRzuEVFVKCmWDBa0ajlvatO1e693cbeoGKbi0yTCzEF1mrN6y3n1npCKIEpoEXs0C3t1OMsR_jDxTPaSptFUuifFBB87Oq3VbVhUh60St_kKlI-SgefpBSqf4ouDNoiAxUf6IPUwhsM2Xz__PXn1bsq-P2K0EE7fBmSlqZ8MSrA4g9y4EL9W9cxix_QLcucH2C8DmBUhlL45dvy-6-_HkN12DBOk</addsrcrecordid><sourcetype>Open Website</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>1326450621</pqid></control><display><type>article</type><title>Tonoplast- and plasma membrane-localized aquaporin-family transporters in blue hydrangea sepals of aluminum hyperaccumulating plant</title><source>MEDLINE</source><source>DOAJ Directory of Open Access Journals</source><source>Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals</source><source>Public Library of Science (PLoS)</source><source>PubMed Central</source><source>Free Full-Text Journals in Chemistry</source><creator>Negishi, Takashi ; Oshima, Kenshiro ; Hattori, Masahira ; Kanai, Masatake ; Mano, Shoji ; Nishimura, Mikio ; Yoshida, Kumi</creator><contributor>Matsui, Minami</contributor><creatorcontrib>Negishi, Takashi ; Oshima, Kenshiro ; Hattori, Masahira ; Kanai, Masatake ; Mano, Shoji ; Nishimura, Mikio ; Yoshida, Kumi ; Matsui, Minami</creatorcontrib><description>Hydrangea (Hydrangea macrophylla) is tolerant of acidic soils in which toxicity generally arises from the presence of the soluble aluminum (Al) ion. When hydrangea is cultivated in acidic soil, its resulting blue sepal color is caused by the Al complex formation of anthocyanin. The concentration of vacuolar Al in blue sepal cells can reach levels in excess of approximately 15 mM, suggesting the existence of an Al-transport and/or storage system. However, until now, no Al transporter has been identified in Al hyperaccumulating plants, animals or microorganisms. To identify the transporter being responsible for Al hyperaccumulation, we prepared a cDNA library from blue sepals according to the sepal maturation stage, and then selected candidate genes using a microarray analysis and an in silico study. Here, we identified the vacuolar and plasma membrane-localized Al transporters genes vacuolar Al transporter (VALT) and plasma membrane Al transporter 1 (PALT1), respectively, which are both members of the aquaporin family. The localization of each protein was confirmed by the transient co-expression of the genes. Reverse transcription-PCR and immunoblotting results indicated that VALT and PALT1 are highly expressed in sepal tissue. The overexpression of VALT and PALT1 in Arabidopsis thaliana conferred Al-tolerance and Al-sensitivity, respectively.</description><identifier>ISSN: 1932-6203</identifier><identifier>EISSN: 1932-6203</identifier><identifier>DOI: 10.1371/journal.pone.0043189</identifier><identifier>PMID: 22952644</identifier><language>eng</language><publisher>United States: Public Library of Science</publisher><subject>Acidic soils ; Aluminum ; Aluminum - chemistry ; Aluminum - metabolism ; Amino Acid Sequence ; Aquaporins ; Aquaporins - metabolism ; Arabidopsis - genetics ; Arabidopsis Proteins - metabolism ; Biological Transport ; Biology ; Boron ; Cell Membrane - metabolism ; Chemistry ; Cloning ; Color ; Complementary DNA ; Complex formation ; DNA microarrays ; DNA, Complementary - metabolism ; Gene expression ; Gene Library ; Genes ; Genetic aspects ; Genomes ; Genomics ; Hydrangea - metabolism ; Hydrangea macrophylla ; Hydrangeas ; Immunoblotting ; Information science ; Ions ; Localization ; Membrane Transport Proteins - metabolism ; Microorganisms ; Models, Genetic ; Molecular Sequence Data ; Mutagenesis ; Phylogeny ; Physiological aspects ; Pigments ; Reverse Transcriptase Polymerase Chain Reaction - methods ; Reverse transcription ; Sepals ; Sequence Homology, Amino Acid ; Toxicity ; Transport proteins</subject><ispartof>PloS one, 2012-08, Vol.7 (8), p.e43189</ispartof><rights>COPYRIGHT 2012 Public Library of Science</rights><rights>Negishi et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License: https://creativecommons.org/licenses/by/4.0/ (the “License”), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><rights>2012 Negishi et al 2012 Negishi et al</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c758t-3a03bd0360a9c76bf175d270a8f1bfa58e7cedd3a3a01de7affa9c04bd7f3c753</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC3430636/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC3430636/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,724,777,781,861,882,2096,2915,23847,27905,27906,53772,53774,79349,79350</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/22952644$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><contributor>Matsui, Minami</contributor><creatorcontrib>Negishi, Takashi</creatorcontrib><creatorcontrib>Oshima, Kenshiro</creatorcontrib><creatorcontrib>Hattori, Masahira</creatorcontrib><creatorcontrib>Kanai, Masatake</creatorcontrib><creatorcontrib>Mano, Shoji</creatorcontrib><creatorcontrib>Nishimura, Mikio</creatorcontrib><creatorcontrib>Yoshida, Kumi</creatorcontrib><title>Tonoplast- and plasma membrane-localized aquaporin-family transporters in blue hydrangea sepals of aluminum hyperaccumulating plant</title><title>PloS one</title><addtitle>PLoS One</addtitle><description>Hydrangea (Hydrangea macrophylla) is tolerant of acidic soils in which toxicity generally arises from the presence of the soluble aluminum (Al) ion. When hydrangea is cultivated in acidic soil, its resulting blue sepal color is caused by the Al complex formation of anthocyanin. The concentration of vacuolar Al in blue sepal cells can reach levels in excess of approximately 15 mM, suggesting the existence of an Al-transport and/or storage system. However, until now, no Al transporter has been identified in Al hyperaccumulating plants, animals or microorganisms. To identify the transporter being responsible for Al hyperaccumulation, we prepared a cDNA library from blue sepals according to the sepal maturation stage, and then selected candidate genes using a microarray analysis and an in silico study. Here, we identified the vacuolar and plasma membrane-localized Al transporters genes vacuolar Al transporter (VALT) and plasma membrane Al transporter 1 (PALT1), respectively, which are both members of the aquaporin family. The localization of each protein was confirmed by the transient co-expression of the genes. Reverse transcription-PCR and immunoblotting results indicated that VALT and PALT1 are highly expressed in sepal tissue. The overexpression of VALT and PALT1 in Arabidopsis thaliana conferred Al-tolerance and Al-sensitivity, respectively.</description><subject>Acidic soils</subject><subject>Aluminum</subject><subject>Aluminum - chemistry</subject><subject>Aluminum - metabolism</subject><subject>Amino Acid Sequence</subject><subject>Aquaporins</subject><subject>Aquaporins - metabolism</subject><subject>Arabidopsis - genetics</subject><subject>Arabidopsis Proteins - metabolism</subject><subject>Biological Transport</subject><subject>Biology</subject><subject>Boron</subject><subject>Cell Membrane - metabolism</subject><subject>Chemistry</subject><subject>Cloning</subject><subject>Color</subject><subject>Complementary DNA</subject><subject>Complex formation</subject><subject>DNA microarrays</subject><subject>DNA, Complementary - metabolism</subject><subject>Gene expression</subject><subject>Gene Library</subject><subject>Genes</subject><subject>Genetic aspects</subject><subject>Genomes</subject><subject>Genomics</subject><subject>Hydrangea - metabolism</subject><subject>Hydrangea macrophylla</subject><subject>Hydrangeas</subject><subject>Immunoblotting</subject><subject>Information science</subject><subject>Ions</subject><subject>Localization</subject><subject>Membrane Transport Proteins - metabolism</subject><subject>Microorganisms</subject><subject>Models, Genetic</subject><subject>Molecular Sequence Data</subject><subject>Mutagenesis</subject><subject>Phylogeny</subject><subject>Physiological aspects</subject><subject>Pigments</subject><subject>Reverse Transcriptase Polymerase Chain Reaction - methods</subject><subject>Reverse transcription</subject><subject>Sepals</subject><subject>Sequence Homology, Amino Acid</subject><subject>Toxicity</subject><subject>Transport proteins</subject><issn>1932-6203</issn><issn>1932-6203</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2012</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><sourceid>DOA</sourceid><recordid>eNqNk12L1DAUhoso7rr6D0QLguBFx6Rp086NsCx-DCws6OptOM1HJ0uadJNUHG_942ac7jIFBclFwjnPec_hJSfLnmO0wqTBb2_c5C2Y1eisXCFUEdyuH2SneE3KgpaIPDx6n2RPQrhBqCYtpY-zk7Jc1yWtqtPs17WzbjQQYpGDFfn-OUA-yKHzYGVhHAejf0qRw-0Eo_PaFgoGbXZ5TEBIkSh9yLXNOzPJfLsTKdxLyIMcwYTcqRzMNGg7DSk5Sg-cT8NkIGrb79vZ-DR7pBIqn833Wfb1w_vri0_F5dXHzcX5ZcGbuo0FAUQ6gQhFsOYN7RRualE2CFqFOwV1KxsuhSCQQCxkA0olEFWdaBRJEuQse3nQHY0LbPYvMEySFTWiJU7E5kAIBzds9HoAv2MONPsTcL5n4KPmRjKFVcdpRzuEVFVKCmWDBa0ajlvatO1e693cbeoGKbi0yTCzEF1mrN6y3n1npCKIEpoEXs0C3t1OMsR_jDxTPaSptFUuifFBB87Oq3VbVhUh60St_kKlI-SgefpBSqf4ouDNoiAxUf6IPUwhsM2Xz__PXn1bsq-P2K0EE7fBmSlqZ8MSrA4g9y4EL9W9cxix_QLcucH2C8DmBUhlL45dvy-6-_HkN12DBOk</recordid><startdate>20120829</startdate><enddate>20120829</enddate><creator>Negishi, Takashi</creator><creator>Oshima, Kenshiro</creator><creator>Hattori, Masahira</creator><creator>Kanai, Masatake</creator><creator>Mano, Shoji</creator><creator>Nishimura, Mikio</creator><creator>Yoshida, Kumi</creator><general>Public Library of Science</general><general>Public Library of Science (PLoS)</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>IOV</scope><scope>ISR</scope><scope>3V.</scope><scope>7QG</scope><scope>7QL</scope><scope>7QO</scope><scope>7RV</scope><scope>7SN</scope><scope>7SS</scope><scope>7T5</scope><scope>7TG</scope><scope>7TM</scope><scope>7U9</scope><scope>7X2</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>8AO</scope><scope>8C1</scope><scope>8FD</scope><scope>8FE</scope><scope>8FG</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABJCF</scope><scope>ABUWG</scope><scope>AEUYN</scope><scope>AFKRA</scope><scope>ARAPS</scope><scope>ATCPS</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BGLVJ</scope><scope>BHPHI</scope><scope>C1K</scope><scope>CCPQU</scope><scope>D1I</scope><scope>DWQXO</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>H94</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>KB.</scope><scope>KB0</scope><scope>KL.</scope><scope>L6V</scope><scope>LK8</scope><scope>M0K</scope><scope>M0S</scope><scope>M1P</scope><scope>M7N</scope><scope>M7P</scope><scope>M7S</scope><scope>NAPCQ</scope><scope>P5Z</scope><scope>P62</scope><scope>P64</scope><scope>PATMY</scope><scope>PDBOC</scope><scope>PIMPY</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>PTHSS</scope><scope>PYCSY</scope><scope>RC3</scope><scope>5PM</scope><scope>DOA</scope></search><sort><creationdate>20120829</creationdate><title>Tonoplast- and plasma membrane-localized aquaporin-family transporters in blue hydrangea sepals of aluminum hyperaccumulating plant</title><author>Negishi, Takashi ; Oshima, Kenshiro ; Hattori, Masahira ; Kanai, Masatake ; Mano, Shoji ; Nishimura, Mikio ; Yoshida, Kumi</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c758t-3a03bd0360a9c76bf175d270a8f1bfa58e7cedd3a3a01de7affa9c04bd7f3c753</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2012</creationdate><topic>Acidic soils</topic><topic>Aluminum</topic><topic>Aluminum - chemistry</topic><topic>Aluminum - metabolism</topic><topic>Amino Acid Sequence</topic><topic>Aquaporins</topic><topic>Aquaporins - metabolism</topic><topic>Arabidopsis - genetics</topic><topic>Arabidopsis Proteins - metabolism</topic><topic>Biological Transport</topic><topic>Biology</topic><topic>Boron</topic><topic>Cell Membrane - metabolism</topic><topic>Chemistry</topic><topic>Cloning</topic><topic>Color</topic><topic>Complementary DNA</topic><topic>Complex formation</topic><topic>DNA microarrays</topic><topic>DNA, Complementary - metabolism</topic><topic>Gene expression</topic><topic>Gene Library</topic><topic>Genes</topic><topic>Genetic aspects</topic><topic>Genomes</topic><topic>Genomics</topic><topic>Hydrangea - metabolism</topic><topic>Hydrangea macrophylla</topic><topic>Hydrangeas</topic><topic>Immunoblotting</topic><topic>Information science</topic><topic>Ions</topic><topic>Localization</topic><topic>Membrane Transport Proteins - metabolism</topic><topic>Microorganisms</topic><topic>Models, Genetic</topic><topic>Molecular Sequence Data</topic><topic>Mutagenesis</topic><topic>Phylogeny</topic><topic>Physiological aspects</topic><topic>Pigments</topic><topic>Reverse Transcriptase Polymerase Chain Reaction - methods</topic><topic>Reverse transcription</topic><topic>Sepals</topic><topic>Sequence Homology, Amino Acid</topic><topic>Toxicity</topic><topic>Transport proteins</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Negishi, Takashi</creatorcontrib><creatorcontrib>Oshima, Kenshiro</creatorcontrib><creatorcontrib>Hattori, Masahira</creatorcontrib><creatorcontrib>Kanai, Masatake</creatorcontrib><creatorcontrib>Mano, Shoji</creatorcontrib><creatorcontrib>Nishimura, Mikio</creatorcontrib><creatorcontrib>Yoshida, Kumi</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Gale In Context: Opposing Viewpoints</collection><collection>Gale In Context: Science</collection><collection>ProQuest Central (Corporate)</collection><collection>Animal Behavior Abstracts</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Biotechnology Research Abstracts</collection><collection>Nursing & Allied Health Database</collection><collection>Ecology Abstracts</collection><collection>Entomology Abstracts (Full archive)</collection><collection>Immunology Abstracts</collection><collection>Meteorological & Geoastrophysical Abstracts</collection><collection>Nucleic Acids Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>Agricultural Science Collection</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Medical Database (Alumni Edition)</collection><collection>ProQuest Pharma Collection</collection><collection>Public Health Database</collection><collection>Technology Research Database</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Technology Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>Materials Science & Engineering Collection</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest One Sustainability</collection><collection>ProQuest Central UK/Ireland</collection><collection>Advanced Technologies & Aerospace Collection</collection><collection>Agricultural & Environmental Science Collection</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Technology Collection</collection><collection>Natural Science Collection</collection><collection>Environmental Sciences and Pollution Management</collection><collection>ProQuest One Community College</collection><collection>ProQuest Materials Science Collection</collection><collection>ProQuest Central Korea</collection><collection>Engineering Research Database</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Materials Science Database</collection><collection>Nursing & Allied Health Database (Alumni Edition)</collection><collection>Meteorological & Geoastrophysical Abstracts - Academic</collection><collection>ProQuest Engineering Collection</collection><collection>ProQuest Biological Science Collection</collection><collection>Agricultural Science Database</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biological Science Database</collection><collection>Engineering Database</collection><collection>Nursing & Allied Health Premium</collection><collection>Advanced Technologies & Aerospace Database</collection><collection>ProQuest Advanced Technologies & Aerospace Collection</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Environmental Science Database</collection><collection>Materials Science Collection</collection><collection>Publicly Available Content Database</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><collection>Engineering Collection</collection><collection>Environmental Science Collection</collection><collection>Genetics Abstracts</collection><collection>PubMed Central (Full Participant titles)</collection><collection>DOAJ Directory of Open Access Journals</collection><jtitle>PloS one</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Negishi, Takashi</au><au>Oshima, Kenshiro</au><au>Hattori, Masahira</au><au>Kanai, Masatake</au><au>Mano, Shoji</au><au>Nishimura, Mikio</au><au>Yoshida, Kumi</au><au>Matsui, Minami</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Tonoplast- and plasma membrane-localized aquaporin-family transporters in blue hydrangea sepals of aluminum hyperaccumulating plant</atitle><jtitle>PloS one</jtitle><addtitle>PLoS One</addtitle><date>2012-08-29</date><risdate>2012</risdate><volume>7</volume><issue>8</issue><spage>e43189</spage><pages>e43189-</pages><issn>1932-6203</issn><eissn>1932-6203</eissn><abstract>Hydrangea (Hydrangea macrophylla) is tolerant of acidic soils in which toxicity generally arises from the presence of the soluble aluminum (Al) ion. When hydrangea is cultivated in acidic soil, its resulting blue sepal color is caused by the Al complex formation of anthocyanin. The concentration of vacuolar Al in blue sepal cells can reach levels in excess of approximately 15 mM, suggesting the existence of an Al-transport and/or storage system. However, until now, no Al transporter has been identified in Al hyperaccumulating plants, animals or microorganisms. To identify the transporter being responsible for Al hyperaccumulation, we prepared a cDNA library from blue sepals according to the sepal maturation stage, and then selected candidate genes using a microarray analysis and an in silico study. Here, we identified the vacuolar and plasma membrane-localized Al transporters genes vacuolar Al transporter (VALT) and plasma membrane Al transporter 1 (PALT1), respectively, which are both members of the aquaporin family. The localization of each protein was confirmed by the transient co-expression of the genes. Reverse transcription-PCR and immunoblotting results indicated that VALT and PALT1 are highly expressed in sepal tissue. The overexpression of VALT and PALT1 in Arabidopsis thaliana conferred Al-tolerance and Al-sensitivity, respectively.</abstract><cop>United States</cop><pub>Public Library of Science</pub><pmid>22952644</pmid><doi>10.1371/journal.pone.0043189</doi><tpages>e43189</tpages><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 1932-6203 |
| ispartof | PloS one, 2012-08, Vol.7 (8), p.e43189 |
| issn | 1932-6203 1932-6203 |
| language | eng |
| recordid | cdi_plos_journals_1326450621 |
| source | MEDLINE; DOAJ Directory of Open Access Journals; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; Public Library of Science (PLoS); PubMed Central; Free Full-Text Journals in Chemistry |
| subjects | Acidic soils Aluminum Aluminum - chemistry Aluminum - metabolism Amino Acid Sequence Aquaporins Aquaporins - metabolism Arabidopsis - genetics Arabidopsis Proteins - metabolism Biological Transport Biology Boron Cell Membrane - metabolism Chemistry Cloning Color Complementary DNA Complex formation DNA microarrays DNA, Complementary - metabolism Gene expression Gene Library Genes Genetic aspects Genomes Genomics Hydrangea - metabolism Hydrangea macrophylla Hydrangeas Immunoblotting Information science Ions Localization Membrane Transport Proteins - metabolism Microorganisms Models, Genetic Molecular Sequence Data Mutagenesis Phylogeny Physiological aspects Pigments Reverse Transcriptase Polymerase Chain Reaction - methods Reverse transcription Sepals Sequence Homology, Amino Acid Toxicity Transport proteins |
| title | Tonoplast- and plasma membrane-localized aquaporin-family transporters in blue hydrangea sepals of aluminum hyperaccumulating plant |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-19T19%3A59%3A29IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-gale_plos_&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Tonoplast-%20and%20plasma%20membrane-localized%20aquaporin-family%20transporters%20in%20blue%20hydrangea%20sepals%20of%20aluminum%20hyperaccumulating%20plant&rft.jtitle=PloS%20one&rft.au=Negishi,%20Takashi&rft.date=2012-08-29&rft.volume=7&rft.issue=8&rft.spage=e43189&rft.pages=e43189-&rft.issn=1932-6203&rft.eissn=1932-6203&rft_id=info:doi/10.1371/journal.pone.0043189&rft_dat=%3Cgale_plos_%3EA498244339%3C/gale_plos_%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=1326450621&rft_id=info:pmid/22952644&rft_galeid=A498244339&rft_doaj_id=oai_doaj_org_article_f1fbc6b6b00f42e6a271d647c1867881&rfr_iscdi=true |