Poor prognosis of gastric adenocarcinoma with decreased expression of AHRR
The aryl hydrocarbon receptor (AHR) repressor (AHRR), a member of growing superfamily, is a basic-helix-loop-helix/Per-AHR nuclear translocator (ARNT)-Sim (bHLH-PAS) protein. Recently, AHRR has been proposed to function as a putative new tumor suppressor gene based on some relevant studies in multip...
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| creator | Li, Yuan-fang Wang, Dan-dan Zhao, Bai-wei Wang, Wei Yuan, Shu-qiang Huang, Chun-yu Chen, Yong-ming Zheng, Yan Keshari, Rajiv Prasad Xia, Jian-chuan Zhou, Zhi-wei |
| description | The aryl hydrocarbon receptor (AHR) repressor (AHRR), a member of growing superfamily, is a basic-helix-loop-helix/Per-AHR nuclear translocator (ARNT)-Sim (bHLH-PAS) protein. Recently, AHRR has been proposed to function as a putative new tumor suppressor gene based on some relevant studies in multiple types of human cancers. This current study aims to investigate AHHR expression and its prognostic significance in primary gastric adenocarcinoma.
The expression level of AHRR was analyzed using real-time quantitative PCR (RT-qPCR), western blotting, and immunohistochemical staining. It was clearly showed that the expression status of AHRR was reduced in tumor tissue samples compared with that in matched adjacent non-tumor tissue samples by RT-qPCR (P = 0.0423) and western blotting analysis (P = 0.004). Moreover, data revealed that AHRR without exon 8 (the active isoform) was the predominant form either in tumor tissues (66.7%, 8/12) or in matched adjacent non-tumor tissues (100.0%, 12/12), and the mRNA level of this isoform was significantly reduced in tumor tissues (P = 0.006). Immunohistochemistry analysis indicated that AHRR expression was significantly decreased in 175 of 410 (42.7%) gastric adenocarcinoma cases. Kaplan-Meier survival curves and Multivariate Cox analysis revealed that decreased expression of AHRR was significantly associated with poor prognosis in gastric adenocarcinoma patients.
Our data suggests that, in primary gastric adenocarcinoma, AHRR may play as a suppressor gene and its expression status has the potential to be an independent prognostic factor. |
| doi_str_mv | 10.1371/journal.pone.0043555 |
| format | Article |
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The expression level of AHRR was analyzed using real-time quantitative PCR (RT-qPCR), western blotting, and immunohistochemical staining. It was clearly showed that the expression status of AHRR was reduced in tumor tissue samples compared with that in matched adjacent non-tumor tissue samples by RT-qPCR (P = 0.0423) and western blotting analysis (P = 0.004). Moreover, data revealed that AHRR without exon 8 (the active isoform) was the predominant form either in tumor tissues (66.7%, 8/12) or in matched adjacent non-tumor tissues (100.0%, 12/12), and the mRNA level of this isoform was significantly reduced in tumor tissues (P = 0.006). Immunohistochemistry analysis indicated that AHRR expression was significantly decreased in 175 of 410 (42.7%) gastric adenocarcinoma cases. Kaplan-Meier survival curves and Multivariate Cox analysis revealed that decreased expression of AHRR was significantly associated with poor prognosis in gastric adenocarcinoma patients.
Our data suggests that, in primary gastric adenocarcinoma, AHRR may play as a suppressor gene and its expression status has the potential to be an independent prognostic factor.</description><identifier>ISSN: 1932-6203</identifier><identifier>EISSN: 1932-6203</identifier><identifier>DOI: 10.1371/journal.pone.0043555</identifier><identifier>PMID: 22952704</identifier><language>eng</language><publisher>United States: Public Library of Science</publisher><subject>Active Transport, Cell Nucleus ; Adenocarcinoma ; Adenocarcinoma - diagnosis ; Aromatic compounds ; Basic Helix-Loop-Helix Transcription Factors - biosynthesis ; Breast cancer ; Cancer therapies ; Exons ; Gastric cancer ; Gene expression ; Gene Expression Profiling ; Gene Expression Regulation, Neoplastic ; Genes ; Genetic aspects ; Helix-loop-helix proteins ; Helix-loop-helix proteins (basic) ; Humans ; Hydrocarbons ; Hypoxia ; Immunohistochemistry ; Immunohistochemistry - methods ; Laboratories ; Liver cancer ; Medical prognosis ; Medicine ; mRNA ; Multivariate Analysis ; Oncology ; Physiological aspects ; Polymerase Chain Reaction ; Prognosis ; Prostate cancer ; Repressor Proteins - biosynthesis ; Retrospective Studies ; RNA, Messenger - metabolism ; Stomach cancer ; Stomach Neoplasms - diagnosis ; Surgery ; Tissues ; Transcription factors ; Tumor suppressor genes ; Tumors ; Western blotting ; Zebrafish</subject><ispartof>PloS one, 2012-08, Vol.7 (8), p.e43555-e43555</ispartof><rights>COPYRIGHT 2012 Public Library of Science</rights><rights>Li et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License: https://creativecommons.org/licenses/by/4.0/ (the “License”), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><rights>2012 Li et al 2012 Li et al</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c692t-cb749010dab316e25bd2c318276a02e1872cf2b12883ccbf0e056772e683e8163</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC3428367/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC3428367/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,727,780,784,864,885,2102,2928,23866,27924,27925,53791,53793,79600,79601</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/22952704$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><contributor>Katoh, Masaru</contributor><creatorcontrib>Li, Yuan-fang</creatorcontrib><creatorcontrib>Wang, Dan-dan</creatorcontrib><creatorcontrib>Zhao, Bai-wei</creatorcontrib><creatorcontrib>Wang, Wei</creatorcontrib><creatorcontrib>Yuan, Shu-qiang</creatorcontrib><creatorcontrib>Huang, Chun-yu</creatorcontrib><creatorcontrib>Chen, Yong-ming</creatorcontrib><creatorcontrib>Zheng, Yan</creatorcontrib><creatorcontrib>Keshari, Rajiv Prasad</creatorcontrib><creatorcontrib>Xia, Jian-chuan</creatorcontrib><creatorcontrib>Zhou, Zhi-wei</creatorcontrib><title>Poor prognosis of gastric adenocarcinoma with decreased expression of AHRR</title><title>PloS one</title><addtitle>PLoS One</addtitle><description>The aryl hydrocarbon receptor (AHR) repressor (AHRR), a member of growing superfamily, is a basic-helix-loop-helix/Per-AHR nuclear translocator (ARNT)-Sim (bHLH-PAS) protein. Recently, AHRR has been proposed to function as a putative new tumor suppressor gene based on some relevant studies in multiple types of human cancers. This current study aims to investigate AHHR expression and its prognostic significance in primary gastric adenocarcinoma.
The expression level of AHRR was analyzed using real-time quantitative PCR (RT-qPCR), western blotting, and immunohistochemical staining. It was clearly showed that the expression status of AHRR was reduced in tumor tissue samples compared with that in matched adjacent non-tumor tissue samples by RT-qPCR (P = 0.0423) and western blotting analysis (P = 0.004). Moreover, data revealed that AHRR without exon 8 (the active isoform) was the predominant form either in tumor tissues (66.7%, 8/12) or in matched adjacent non-tumor tissues (100.0%, 12/12), and the mRNA level of this isoform was significantly reduced in tumor tissues (P = 0.006). Immunohistochemistry analysis indicated that AHRR expression was significantly decreased in 175 of 410 (42.7%) gastric adenocarcinoma cases. Kaplan-Meier survival curves and Multivariate Cox analysis revealed that decreased expression of AHRR was significantly associated with poor prognosis in gastric adenocarcinoma patients.
Our data suggests that, in primary gastric adenocarcinoma, AHRR may play as a suppressor gene and its expression status has the potential to be an independent prognostic factor.</description><subject>Active Transport, Cell Nucleus</subject><subject>Adenocarcinoma</subject><subject>Adenocarcinoma - diagnosis</subject><subject>Aromatic compounds</subject><subject>Basic Helix-Loop-Helix Transcription Factors - biosynthesis</subject><subject>Breast cancer</subject><subject>Cancer therapies</subject><subject>Exons</subject><subject>Gastric cancer</subject><subject>Gene expression</subject><subject>Gene Expression Profiling</subject><subject>Gene Expression Regulation, Neoplastic</subject><subject>Genes</subject><subject>Genetic aspects</subject><subject>Helix-loop-helix proteins</subject><subject>Helix-loop-helix proteins (basic)</subject><subject>Humans</subject><subject>Hydrocarbons</subject><subject>Hypoxia</subject><subject>Immunohistochemistry</subject><subject>Immunohistochemistry - methods</subject><subject>Laboratories</subject><subject>Liver cancer</subject><subject>Medical prognosis</subject><subject>Medicine</subject><subject>mRNA</subject><subject>Multivariate Analysis</subject><subject>Oncology</subject><subject>Physiological aspects</subject><subject>Polymerase Chain Reaction</subject><subject>Prognosis</subject><subject>Prostate cancer</subject><subject>Repressor Proteins - biosynthesis</subject><subject>Retrospective Studies</subject><subject>RNA, Messenger - metabolism</subject><subject>Stomach cancer</subject><subject>Stomach Neoplasms - diagnosis</subject><subject>Surgery</subject><subject>Tissues</subject><subject>Transcription factors</subject><subject>Tumor suppressor genes</subject><subject>Tumors</subject><subject>Western blotting</subject><subject>Zebrafish</subject><issn>1932-6203</issn><issn>1932-6203</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2012</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><sourceid>DOA</sourceid><recordid>eNqNkl2L1DAUhoso7rr6D0QLgujFjPlo0vRGGBZ1RxZWxo_bkKSnnQydppu0uv57U6e7TGUvJBcJyfO-Jzl5k-Q5RktMc_xu5wbfqmbZuRaWCGWUMfYgOcUFJQtOEH14tD5JnoSwQ4hRwfnj5ISQgpEcZafJ5y_O-bTzrm5dsCF1VVqr0HtrUlVC64zyxrZur9Jftt-mJRgPKkCZwk3nIQTr2lGzuthsniaPKtUEeDbNZ8n3jx--nV8sLq8-rc9XlwvDC9IvjM6zAmFUKk0xB8J0SQzFguRcIQJY5MRURGMiBDVGVwgQ43lOgAsKAnN6lrw8-HaNC3JqQ5CYEp4xxBiNxPpAlE7tZOftXvnf0ikr_244X0vle2sakJUpVBFLi0KLjAHRWiFUYM0roJqXOHq9n6oNeg-lgbb3qpmZzk9au5W1-ylpRgTleTR4Mxl4dz1A6OXeBgNNo1pwQ7w3ooIVEWURffUPev_rJqpW8QG2rVysa0ZTucoKQSKWj_de3kPFUcLempiZysb9meDtTBCZHm76Wg0hyPXXzf-zVz_m7Osjdguq6bfBNUMfoxPmYHYAjXcheKjumoyRHCN_2w05Rl5OkY-yF8cfdCe6zTj9A24c-jc</recordid><startdate>20120827</startdate><enddate>20120827</enddate><creator>Li, Yuan-fang</creator><creator>Wang, Dan-dan</creator><creator>Zhao, Bai-wei</creator><creator>Wang, Wei</creator><creator>Yuan, Shu-qiang</creator><creator>Huang, Chun-yu</creator><creator>Chen, Yong-ming</creator><creator>Zheng, Yan</creator><creator>Keshari, Rajiv Prasad</creator><creator>Xia, Jian-chuan</creator><creator>Zhou, Zhi-wei</creator><general>Public Library of Science</general><general>Public Library of Science (PLoS)</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>IOV</scope><scope>ISR</scope><scope>3V.</scope><scope>7QG</scope><scope>7QL</scope><scope>7QO</scope><scope>7RV</scope><scope>7SN</scope><scope>7SS</scope><scope>7T5</scope><scope>7TG</scope><scope>7TM</scope><scope>7U9</scope><scope>7X2</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>8AO</scope><scope>8C1</scope><scope>8FD</scope><scope>8FE</scope><scope>8FG</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABJCF</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>ARAPS</scope><scope>ATCPS</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BGLVJ</scope><scope>BHPHI</scope><scope>C1K</scope><scope>CCPQU</scope><scope>D1I</scope><scope>DWQXO</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>H94</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>KB.</scope><scope>KB0</scope><scope>KL.</scope><scope>L6V</scope><scope>LK8</scope><scope>M0K</scope><scope>M0S</scope><scope>M1P</scope><scope>M7N</scope><scope>M7P</scope><scope>M7S</scope><scope>NAPCQ</scope><scope>P5Z</scope><scope>P62</scope><scope>P64</scope><scope>PATMY</scope><scope>PDBOC</scope><scope>PIMPY</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PTHSS</scope><scope>PYCSY</scope><scope>RC3</scope><scope>7X8</scope><scope>5PM</scope><scope>DOA</scope></search><sort><creationdate>20120827</creationdate><title>Poor prognosis of gastric adenocarcinoma with decreased expression of AHRR</title><author>Li, Yuan-fang ; Wang, Dan-dan ; Zhao, Bai-wei ; Wang, Wei ; Yuan, Shu-qiang ; Huang, Chun-yu ; Chen, Yong-ming ; Zheng, Yan ; Keshari, Rajiv Prasad ; Xia, Jian-chuan ; Zhou, Zhi-wei</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c692t-cb749010dab316e25bd2c318276a02e1872cf2b12883ccbf0e056772e683e8163</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2012</creationdate><topic>Active Transport, Cell Nucleus</topic><topic>Adenocarcinoma</topic><topic>Adenocarcinoma - diagnosis</topic><topic>Aromatic compounds</topic><topic>Basic Helix-Loop-Helix Transcription Factors - biosynthesis</topic><topic>Breast cancer</topic><topic>Cancer therapies</topic><topic>Exons</topic><topic>Gastric cancer</topic><topic>Gene expression</topic><topic>Gene Expression Profiling</topic><topic>Gene Expression Regulation, Neoplastic</topic><topic>Genes</topic><topic>Genetic aspects</topic><topic>Helix-loop-helix proteins</topic><topic>Helix-loop-helix proteins (basic)</topic><topic>Humans</topic><topic>Hydrocarbons</topic><topic>Hypoxia</topic><topic>Immunohistochemistry</topic><topic>Immunohistochemistry - methods</topic><topic>Laboratories</topic><topic>Liver cancer</topic><topic>Medical prognosis</topic><topic>Medicine</topic><topic>mRNA</topic><topic>Multivariate Analysis</topic><topic>Oncology</topic><topic>Physiological aspects</topic><topic>Polymerase Chain Reaction</topic><topic>Prognosis</topic><topic>Prostate cancer</topic><topic>Repressor Proteins - biosynthesis</topic><topic>Retrospective Studies</topic><topic>RNA, Messenger - 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Academic</collection><collection>PubMed Central (Full Participant titles)</collection><collection>DOAJ Directory of Open Access Journals</collection><jtitle>PloS one</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Li, Yuan-fang</au><au>Wang, Dan-dan</au><au>Zhao, Bai-wei</au><au>Wang, Wei</au><au>Yuan, Shu-qiang</au><au>Huang, Chun-yu</au><au>Chen, Yong-ming</au><au>Zheng, Yan</au><au>Keshari, Rajiv Prasad</au><au>Xia, Jian-chuan</au><au>Zhou, Zhi-wei</au><au>Katoh, Masaru</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Poor prognosis of gastric adenocarcinoma with decreased expression of AHRR</atitle><jtitle>PloS one</jtitle><addtitle>PLoS One</addtitle><date>2012-08-27</date><risdate>2012</risdate><volume>7</volume><issue>8</issue><spage>e43555</spage><epage>e43555</epage><pages>e43555-e43555</pages><issn>1932-6203</issn><eissn>1932-6203</eissn><abstract>The aryl hydrocarbon receptor (AHR) repressor (AHRR), a member of growing superfamily, is a basic-helix-loop-helix/Per-AHR nuclear translocator (ARNT)-Sim (bHLH-PAS) protein. Recently, AHRR has been proposed to function as a putative new tumor suppressor gene based on some relevant studies in multiple types of human cancers. This current study aims to investigate AHHR expression and its prognostic significance in primary gastric adenocarcinoma.
The expression level of AHRR was analyzed using real-time quantitative PCR (RT-qPCR), western blotting, and immunohistochemical staining. It was clearly showed that the expression status of AHRR was reduced in tumor tissue samples compared with that in matched adjacent non-tumor tissue samples by RT-qPCR (P = 0.0423) and western blotting analysis (P = 0.004). Moreover, data revealed that AHRR without exon 8 (the active isoform) was the predominant form either in tumor tissues (66.7%, 8/12) or in matched adjacent non-tumor tissues (100.0%, 12/12), and the mRNA level of this isoform was significantly reduced in tumor tissues (P = 0.006). Immunohistochemistry analysis indicated that AHRR expression was significantly decreased in 175 of 410 (42.7%) gastric adenocarcinoma cases. Kaplan-Meier survival curves and Multivariate Cox analysis revealed that decreased expression of AHRR was significantly associated with poor prognosis in gastric adenocarcinoma patients.
Our data suggests that, in primary gastric adenocarcinoma, AHRR may play as a suppressor gene and its expression status has the potential to be an independent prognostic factor.</abstract><cop>United States</cop><pub>Public Library of Science</pub><pmid>22952704</pmid><doi>10.1371/journal.pone.0043555</doi><tpages>e43555</tpages><oa>free_for_read</oa></addata></record> |
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| identifier | ISSN: 1932-6203 |
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| issn | 1932-6203 1932-6203 |
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| source | MEDLINE; DOAJ Directory of Open Access Journals; Public Library of Science (PLoS) Journals Open Access; EZB-FREE-00999 freely available EZB journals; PubMed Central; Free Full-Text Journals in Chemistry |
| subjects | Active Transport, Cell Nucleus Adenocarcinoma Adenocarcinoma - diagnosis Aromatic compounds Basic Helix-Loop-Helix Transcription Factors - biosynthesis Breast cancer Cancer therapies Exons Gastric cancer Gene expression Gene Expression Profiling Gene Expression Regulation, Neoplastic Genes Genetic aspects Helix-loop-helix proteins Helix-loop-helix proteins (basic) Humans Hydrocarbons Hypoxia Immunohistochemistry Immunohistochemistry - methods Laboratories Liver cancer Medical prognosis Medicine mRNA Multivariate Analysis Oncology Physiological aspects Polymerase Chain Reaction Prognosis Prostate cancer Repressor Proteins - biosynthesis Retrospective Studies RNA, Messenger - metabolism Stomach cancer Stomach Neoplasms - diagnosis Surgery Tissues Transcription factors Tumor suppressor genes Tumors Western blotting Zebrafish |
| title | Poor prognosis of gastric adenocarcinoma with decreased expression of AHRR |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2024-12-29T00%3A45%3A27IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-gale_plos_&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Poor%20prognosis%20of%20gastric%20adenocarcinoma%20with%20decreased%20expression%20of%20AHRR&rft.jtitle=PloS%20one&rft.au=Li,%20Yuan-fang&rft.date=2012-08-27&rft.volume=7&rft.issue=8&rft.spage=e43555&rft.epage=e43555&rft.pages=e43555-e43555&rft.issn=1932-6203&rft.eissn=1932-6203&rft_id=info:doi/10.1371/journal.pone.0043555&rft_dat=%3Cgale_plos_%3EA498245071%3C/gale_plos_%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=1326450553&rft_id=info:pmid/22952704&rft_galeid=A498245071&rft_doaj_id=oai_doaj_org_article_fc9a927689b845e2bba0091b6fe3b6d1&rfr_iscdi=true |