Androgen concentrations in umbilical cord blood and their association with maternal, fetal and obstetric factors
The aim of this study was to measure umbilical blood androgen concentrations in a birth cohort using a highly specific liquid chromatography-tandem mass spectrometry (LC-MS/MS) assay and assesses the effects of sex, labor, and gestational age on fetal androgen levels at birth. We performed a prospec...
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| Veröffentlicht in: | PloS one 2012-08, Vol.7 (8), p.e42827-e42827 |
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| creator | Keelan, Jeffrey A Mattes, Eugen Tan, HaiWei Dinan, Andrew Newnham, John P Whitehouse, Andrew J O Jacoby, Peter Hickey, Martha |
| description | The aim of this study was to measure umbilical blood androgen concentrations in a birth cohort using a highly specific liquid chromatography-tandem mass spectrometry (LC-MS/MS) assay and assesses the effects of sex, labor, and gestational age on fetal androgen levels at birth. We performed a prospective cohort study of androgen concentrations in mixed arterial and venous umbilical cord serum from 803 unselected singleton pregnancies from a general obstetric population in Western Australia. Total testosterone (TT), Δ4-androstenedione, and dehydroepiandrosterone were extracted from archived cord serum samples and measured using LC-MS/MS. SHBG was measured by ELISA; free testosterone (FT) and bioavailable testosterone (BioT) values were also calculated. Median values for all three androgens were generally lower than previously published values. Levels of TT, FT, BioT, and SHBG were significantly higher in male verses female neonates (P |
| doi_str_mv | 10.1371/journal.pone.0042827 |
| format | Article |
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We performed a prospective cohort study of androgen concentrations in mixed arterial and venous umbilical cord serum from 803 unselected singleton pregnancies from a general obstetric population in Western Australia. Total testosterone (TT), Δ4-androstenedione, and dehydroepiandrosterone were extracted from archived cord serum samples and measured using LC-MS/MS. SHBG was measured by ELISA; free testosterone (FT) and bioavailable testosterone (BioT) values were also calculated. Median values for all three androgens were generally lower than previously published values. Levels of TT, FT, BioT, and SHBG were significantly higher in male verses female neonates (P<0.0001), while dehydroepiandrosterone levels were higher in females (P<0.0001). Labor was associated with a significant (∼15-26%) decrease in median cord blood TT and FT levels (both sexes combined), but a modest (∼16-31%) increase in SHBG, Δ4-androstenedione, and dehydroepiandrosterone concentrations. TT and FT were significantly negatively correlated with gestational age at delivery, while SHBG, Δ4-androstenedione, and dehydroepiandrosterone were positively correlated. Antenatal glucocorticoid administration also had a significant effect in the multiple regression models. This is the first study to report umbilical cord androgen levels in a large unselected population of neonates using LC-MS/MS. Our findings suggest that previous studies have over-estimated cord androgen levels, and that fetal, maternal, and obstetric factors influence cord androgen levels differentially. Caution should be exercised when interpreting previously-published data that have not taken all of these factors into account.</description><identifier>ISSN: 1932-6203</identifier><identifier>EISSN: 1932-6203</identifier><identifier>DOI: 10.1371/journal.pone.0042827</identifier><identifier>PMID: 22916165</identifier><language>eng</language><publisher>United States: Public Library of Science</publisher><subject>Accuracy ; Adult ; Age ; Androgens ; Androgens - blood ; Androstenedione ; Bioavailability ; Biomarkers ; Blood ; Cancer ; Childbirth & labor ; Childrens health ; Chromatography ; Chromatography, Liquid ; Cord blood ; Dehydroepiandrosterone ; Enzyme-Linked Immunosorbent Assay ; Ethnicity ; Family medical history ; Female ; Females ; Fetal Blood - metabolism ; Fetus - metabolism ; Fetuses ; Gestational age ; Glucocorticoids ; Hormones ; Humans ; Infant, Newborn ; Labor ; Liquid chromatography ; Male ; Mass spectrometry ; Mass spectroscopy ; Medical research ; Medicine ; Multiple regression models ; Neonates ; Obstetrics ; Placenta ; Pregnancy ; Prospective Studies ; Regression analysis ; Regression models ; Scientific imaging ; Studies ; Tandem Mass Spectrometry ; Testosterone ; Ultrasonic imaging ; Umbilical cord ; Womens health</subject><ispartof>PloS one, 2012-08, Vol.7 (8), p.e42827-e42827</ispartof><rights>COPYRIGHT 2012 Public Library of Science</rights><rights>Keelan et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License: https://creativecommons.org/licenses/by/4.0/ (the “License”), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><rights>2012 Keelan et al 2012 Keelan et al</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c692t-d5a7f4a6a9649482c67a10319f5dd5b366a59a1a8583bd73c87f83697daaef713</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC3423422/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC3423422/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,724,777,781,861,882,2096,2915,23847,27905,27906,53772,53774,79349,79350</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/22916165$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><contributor>Sun, Kang</contributor><creatorcontrib>Keelan, Jeffrey A</creatorcontrib><creatorcontrib>Mattes, Eugen</creatorcontrib><creatorcontrib>Tan, HaiWei</creatorcontrib><creatorcontrib>Dinan, Andrew</creatorcontrib><creatorcontrib>Newnham, John P</creatorcontrib><creatorcontrib>Whitehouse, Andrew J O</creatorcontrib><creatorcontrib>Jacoby, Peter</creatorcontrib><creatorcontrib>Hickey, Martha</creatorcontrib><title>Androgen concentrations in umbilical cord blood and their association with maternal, fetal and obstetric factors</title><title>PloS one</title><addtitle>PLoS One</addtitle><description>The aim of this study was to measure umbilical blood androgen concentrations in a birth cohort using a highly specific liquid chromatography-tandem mass spectrometry (LC-MS/MS) assay and assesses the effects of sex, labor, and gestational age on fetal androgen levels at birth. We performed a prospective cohort study of androgen concentrations in mixed arterial and venous umbilical cord serum from 803 unselected singleton pregnancies from a general obstetric population in Western Australia. Total testosterone (TT), Δ4-androstenedione, and dehydroepiandrosterone were extracted from archived cord serum samples and measured using LC-MS/MS. SHBG was measured by ELISA; free testosterone (FT) and bioavailable testosterone (BioT) values were also calculated. Median values for all three androgens were generally lower than previously published values. Levels of TT, FT, BioT, and SHBG were significantly higher in male verses female neonates (P<0.0001), while dehydroepiandrosterone levels were higher in females (P<0.0001). Labor was associated with a significant (∼15-26%) decrease in median cord blood TT and FT levels (both sexes combined), but a modest (∼16-31%) increase in SHBG, Δ4-androstenedione, and dehydroepiandrosterone concentrations. TT and FT were significantly negatively correlated with gestational age at delivery, while SHBG, Δ4-androstenedione, and dehydroepiandrosterone were positively correlated. Antenatal glucocorticoid administration also had a significant effect in the multiple regression models. This is the first study to report umbilical cord androgen levels in a large unselected population of neonates using LC-MS/MS. Our findings suggest that previous studies have over-estimated cord androgen levels, and that fetal, maternal, and obstetric factors influence cord androgen levels differentially. Caution should be exercised when interpreting previously-published data that have not taken all of these factors into account.</description><subject>Accuracy</subject><subject>Adult</subject><subject>Age</subject><subject>Androgens</subject><subject>Androgens - blood</subject><subject>Androstenedione</subject><subject>Bioavailability</subject><subject>Biomarkers</subject><subject>Blood</subject><subject>Cancer</subject><subject>Childbirth & labor</subject><subject>Childrens health</subject><subject>Chromatography</subject><subject>Chromatography, Liquid</subject><subject>Cord blood</subject><subject>Dehydroepiandrosterone</subject><subject>Enzyme-Linked Immunosorbent Assay</subject><subject>Ethnicity</subject><subject>Family medical history</subject><subject>Female</subject><subject>Females</subject><subject>Fetal Blood - metabolism</subject><subject>Fetus - metabolism</subject><subject>Fetuses</subject><subject>Gestational age</subject><subject>Glucocorticoids</subject><subject>Hormones</subject><subject>Humans</subject><subject>Infant, Newborn</subject><subject>Labor</subject><subject>Liquid chromatography</subject><subject>Male</subject><subject>Mass spectrometry</subject><subject>Mass spectroscopy</subject><subject>Medical research</subject><subject>Medicine</subject><subject>Multiple regression models</subject><subject>Neonates</subject><subject>Obstetrics</subject><subject>Placenta</subject><subject>Pregnancy</subject><subject>Prospective Studies</subject><subject>Regression analysis</subject><subject>Regression models</subject><subject>Scientific imaging</subject><subject>Studies</subject><subject>Tandem Mass Spectrometry</subject><subject>Testosterone</subject><subject>Ultrasonic imaging</subject><subject>Umbilical cord</subject><subject>Womens 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concentrations in umbilical cord blood and their association with maternal, fetal and obstetric factors</title><author>Keelan, Jeffrey A ; Mattes, Eugen ; Tan, HaiWei ; Dinan, Andrew ; Newnham, John P ; Whitehouse, Andrew J O ; Jacoby, Peter ; Hickey, Martha</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c692t-d5a7f4a6a9649482c67a10319f5dd5b366a59a1a8583bd73c87f83697daaef713</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2012</creationdate><topic>Accuracy</topic><topic>Adult</topic><topic>Age</topic><topic>Androgens</topic><topic>Androgens - blood</topic><topic>Androstenedione</topic><topic>Bioavailability</topic><topic>Biomarkers</topic><topic>Blood</topic><topic>Cancer</topic><topic>Childbirth & labor</topic><topic>Childrens health</topic><topic>Chromatography</topic><topic>Chromatography, Liquid</topic><topic>Cord blood</topic><topic>Dehydroepiandrosterone</topic><topic>Enzyme-Linked Immunosorbent Assay</topic><topic>Ethnicity</topic><topic>Family medical history</topic><topic>Female</topic><topic>Females</topic><topic>Fetal Blood - metabolism</topic><topic>Fetus - metabolism</topic><topic>Fetuses</topic><topic>Gestational age</topic><topic>Glucocorticoids</topic><topic>Hormones</topic><topic>Humans</topic><topic>Infant, Newborn</topic><topic>Labor</topic><topic>Liquid chromatography</topic><topic>Male</topic><topic>Mass spectrometry</topic><topic>Mass spectroscopy</topic><topic>Medical research</topic><topic>Medicine</topic><topic>Multiple regression models</topic><topic>Neonates</topic><topic>Obstetrics</topic><topic>Placenta</topic><topic>Pregnancy</topic><topic>Prospective Studies</topic><topic>Regression analysis</topic><topic>Regression models</topic><topic>Scientific imaging</topic><topic>Studies</topic><topic>Tandem Mass Spectrometry</topic><topic>Testosterone</topic><topic>Ultrasonic imaging</topic><topic>Umbilical cord</topic><topic>Womens 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Participant titles)</collection><collection>DOAJ Directory of Open Access Journals</collection><jtitle>PloS one</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Keelan, Jeffrey A</au><au>Mattes, Eugen</au><au>Tan, HaiWei</au><au>Dinan, Andrew</au><au>Newnham, John P</au><au>Whitehouse, Andrew J O</au><au>Jacoby, Peter</au><au>Hickey, Martha</au><au>Sun, Kang</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Androgen concentrations in umbilical cord blood and their association with maternal, fetal and obstetric factors</atitle><jtitle>PloS one</jtitle><addtitle>PLoS One</addtitle><date>2012-08-20</date><risdate>2012</risdate><volume>7</volume><issue>8</issue><spage>e42827</spage><epage>e42827</epage><pages>e42827-e42827</pages><issn>1932-6203</issn><eissn>1932-6203</eissn><abstract>The aim of this study was to measure umbilical blood androgen concentrations in a birth cohort using a highly specific liquid chromatography-tandem mass spectrometry (LC-MS/MS) assay and assesses the effects of sex, labor, and gestational age on fetal androgen levels at birth. We performed a prospective cohort study of androgen concentrations in mixed arterial and venous umbilical cord serum from 803 unselected singleton pregnancies from a general obstetric population in Western Australia. Total testosterone (TT), Δ4-androstenedione, and dehydroepiandrosterone were extracted from archived cord serum samples and measured using LC-MS/MS. SHBG was measured by ELISA; free testosterone (FT) and bioavailable testosterone (BioT) values were also calculated. Median values for all three androgens were generally lower than previously published values. Levels of TT, FT, BioT, and SHBG were significantly higher in male verses female neonates (P<0.0001), while dehydroepiandrosterone levels were higher in females (P<0.0001). Labor was associated with a significant (∼15-26%) decrease in median cord blood TT and FT levels (both sexes combined), but a modest (∼16-31%) increase in SHBG, Δ4-androstenedione, and dehydroepiandrosterone concentrations. TT and FT were significantly negatively correlated with gestational age at delivery, while SHBG, Δ4-androstenedione, and dehydroepiandrosterone were positively correlated. Antenatal glucocorticoid administration also had a significant effect in the multiple regression models. This is the first study to report umbilical cord androgen levels in a large unselected population of neonates using LC-MS/MS. Our findings suggest that previous studies have over-estimated cord androgen levels, and that fetal, maternal, and obstetric factors influence cord androgen levels differentially. Caution should be exercised when interpreting previously-published data that have not taken all of these factors into account.</abstract><cop>United States</cop><pub>Public Library of Science</pub><pmid>22916165</pmid><doi>10.1371/journal.pone.0042827</doi><tpages>e42827</tpages><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 1932-6203 |
| ispartof | PloS one, 2012-08, Vol.7 (8), p.e42827-e42827 |
| issn | 1932-6203 1932-6203 |
| language | eng |
| recordid | cdi_plos_journals_1326267605 |
| source | MEDLINE; DOAJ Directory of Open Access Journals; Public Library of Science (PLoS); EZB-FREE-00999 freely available EZB journals; PubMed Central; Free Full-Text Journals in Chemistry |
| subjects | Accuracy Adult Age Androgens Androgens - blood Androstenedione Bioavailability Biomarkers Blood Cancer Childbirth & labor Childrens health Chromatography Chromatography, Liquid Cord blood Dehydroepiandrosterone Enzyme-Linked Immunosorbent Assay Ethnicity Family medical history Female Females Fetal Blood - metabolism Fetus - metabolism Fetuses Gestational age Glucocorticoids Hormones Humans Infant, Newborn Labor Liquid chromatography Male Mass spectrometry Mass spectroscopy Medical research Medicine Multiple regression models Neonates Obstetrics Placenta Pregnancy Prospective Studies Regression analysis Regression models Scientific imaging Studies Tandem Mass Spectrometry Testosterone Ultrasonic imaging Umbilical cord Womens health |
| title | Androgen concentrations in umbilical cord blood and their association with maternal, fetal and obstetric factors |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-21T06%3A03%3A08IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-gale_plos_&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Androgen%20concentrations%20in%20umbilical%20cord%20blood%20and%20their%20association%20with%20maternal,%20fetal%20and%20obstetric%20factors&rft.jtitle=PloS%20one&rft.au=Keelan,%20Jeffrey%20A&rft.date=2012-08-20&rft.volume=7&rft.issue=8&rft.spage=e42827&rft.epage=e42827&rft.pages=e42827-e42827&rft.issn=1932-6203&rft.eissn=1932-6203&rft_id=info:doi/10.1371/journal.pone.0042827&rft_dat=%3Cgale_plos_%3EA498261997%3C/gale_plos_%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=1326267605&rft_id=info:pmid/22916165&rft_galeid=A498261997&rft_doaj_id=oai_doaj_org_article_ea8a0bc054154b5d84afd256aae08713&rfr_iscdi=true |