Transformation with oligonucleotides creating clustered changes in the yeast genome

We have studied single-strand oligonucleotide (oligo) transformation of yeast by using 40-nt long oligos that create multiple base changes to the yeast genome spread throughout the length of the oligos, making it possible to measure the portions of an oligo that are incorporated during transformatio...

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Veröffentlicht in:	PloS one 2012-08, Vol.7 (8), p.e42905-e42905
Hauptverfasser:	Rodriguez, Gina P, Song, Joseph B, Crouse, Gray F
Format:	Artikel
Sprache:	eng
Schlagworte:	Baking yeast Base Pair Mismatch Biology Deoxyribonucleic acid DNA DNA biosynthesis DNA polymerase DNA Repair DNA replication E coli Experiments Genome, Fungal Genomes Genomics Mismatch repair Mutation Oligonucleotides Oligonucleotides - genetics Phosphorothioate Polymerase Chain Reaction Saccharomyces cerevisiae Transformation Transformation, Genetic Yeast Yeasts Yeasts - genetics
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