Whole-genome synthesis and characterization of viable S13-like bacteriophages

Unprecedented progresses in high-throughput DNA sequencing and de novo gene synthesis technologies have allowed us to create living organisms in the absence of natural template. The sequence of wild-type S13 phage genome was downloaded from GenBank. Two synonymous mutations were introduced into wt-S...

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Veröffentlicht in:	PloS one 2012-07, Vol.7 (7), p.e41124-e41124
Hauptverfasser:	Liu, Yuchen, Han, Yonghua, Huang, Weiren, Duan, Yonggang, Mou, Lisha, Jiang, Zhimao, Fa, Pingping, Xie, Jun, Diao, Ruiying, Chen, Yuanbin, Ye, Yiwang, Yang, Ruilin, Chen, Jing, Sun, Xiaojuan, Li, Zesong, Tang, Aifa, Gui, Yaoting, Cai, Zhiming
Format:	Artikel
Sprache:	eng
Schlagworte:	Adsorption Bacteria Bacteriophages - genetics Biology Biotechnology Calcium Calcium - chemistry Calcium chloride Capsid protein Chemical synthesis Chimeras Chlamydia Deoxyribonucleic acid DNA DNA polymerase DNA sequencing E coli Electroporation Environmental conditions Escherichia coli - metabolism Escherichia coli - virology Evolution Gene sequencing Gene Transfer, Horizontal Genes Genetic aspects Genetic Engineering - methods Genetic Markers Genetically modified organisms Genetics Genome, Bacterial Genome, Viral Genomes Genomics Homologous recombination Homology Hospitals Hydrogen-Ion Concentration Laboratories Life cycle engineering Life cycles Medicine Models, Genetic Morphogenesis Mutation Nucleotide sequence Oligonucleotides Open Reading Frames Phages Phylogeny Plaques Proteins Reproductive health Sequence Analysis, DNA Synthesis Temperature Ultraviolet Rays Urology Viral proteins
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