Simultaneous detection and identification of enteric viruses by PCR-mass assay
Simultaneous detection of enteric viruses that cause similar symptoms (e.g. hand, foot and mouth disease) is essential to the prevention of outbreaks and control of infections. In this study, a novel PCR-Mass assay combining multiplex polymerase chain reaction (PCR) with matrix-assisted laser desorp...
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| Veröffentlicht in: | PloS one 2012-08, Vol.7 (8), p.e42251-e42251 |
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| creator | Piao, Jingzi Jiang, Jun Xu, Bianli Wang, Xiaohong Guan, Yanfang Wu, Weili Liu, Licheng Zhang, Yuan Huang, Xueyong Wang, Pengzhi Zhao, Jinyin Kang, Xiaoping Jiang, Hua Cao, Yuanyin Zheng, Yuling Jiang, Yongqiang Li, Yan Yang, Yinhui Chen, Weijun |
| description | Simultaneous detection of enteric viruses that cause similar symptoms (e.g. hand, foot and mouth disease) is essential to the prevention of outbreaks and control of infections. In this study, a novel PCR-Mass assay combining multiplex polymerase chain reaction (PCR) with matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) was developed and used for simultaneous detection of eight distinct human enteric viruses. Enteric viral isolates and standard viral RNAs were examined to determine the sensitivity and specificity of the PCR-Mass assay. Clinical performance was evaluated with a total of 101 clinical specimens from patients suspected of having hand, foot and mouth disease (HFMD). The results were compared to those of previous analyses using real-time RT-PCR. The identification of specific viruses and clinical specimens shows that the PCR-Mass assay performed as well as or better than standard methods with respect to indicating the presence of multiplex pathogens in a single specimen. |
| doi_str_mv | 10.1371/journal.pone.0042251 |
| format | Article |
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In this study, a novel PCR-Mass assay combining multiplex polymerase chain reaction (PCR) with matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) was developed and used for simultaneous detection of eight distinct human enteric viruses. Enteric viral isolates and standard viral RNAs were examined to determine the sensitivity and specificity of the PCR-Mass assay. Clinical performance was evaluated with a total of 101 clinical specimens from patients suspected of having hand, foot and mouth disease (HFMD). The results were compared to those of previous analyses using real-time RT-PCR. The identification of specific viruses and clinical specimens shows that the PCR-Mass assay performed as well as or better than standard methods with respect to indicating the presence of multiplex pathogens in a single specimen.</description><identifier>ISSN: 1932-6203</identifier><identifier>EISSN: 1932-6203</identifier><identifier>DOI: 10.1371/journal.pone.0042251</identifier><identifier>PMID: 22870310</identifier><language>eng</language><publisher>United States: Public Library of Science</publisher><subject>Assaying ; Biology ; Coxsackievirus infections ; Desorption ; Disease control ; Echovirus ; Epidemics ; Female ; Foot and mouth disease ; Hand-foot-and-mouth disease ; Health aspects ; Human papillomavirus ; Humans ; Infection ; Infection control ; Ionization ; Ions ; Lasers ; Male ; Mass spectrometry ; Mass spectroscopy ; Medical research ; Medicine ; Multiplex Polymerase Chain Reaction - methods ; Multiplexing ; Outbreaks ; Polymerase chain reaction ; RNA Virus Infections - diagnosis ; RNA Virus Infections - genetics ; RNA Virus Infections - metabolism ; RNA Viruses - genetics ; RNA Viruses - metabolism ; RNA, Viral - genetics ; RNA, Viral - metabolism ; Sensitivity analysis ; Viral infections ; Viruses</subject><ispartof>PloS one, 2012-08, Vol.7 (8), p.e42251-e42251</ispartof><rights>COPYRIGHT 2012 Public Library of Science</rights><rights>Piao et al. 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Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><rights>2012 Piao et al 2012 Piao et al</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c692t-d4825a910ee9d808d59bf42dda3e4035ffc030b621ff3deee20c1b435e5815833</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC3411642/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC3411642/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,727,780,784,864,885,2102,2928,23866,27924,27925,53791,53793,79600,79601</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/22870310$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><contributor>Drews, Steven J.</contributor><creatorcontrib>Piao, Jingzi</creatorcontrib><creatorcontrib>Jiang, Jun</creatorcontrib><creatorcontrib>Xu, Bianli</creatorcontrib><creatorcontrib>Wang, Xiaohong</creatorcontrib><creatorcontrib>Guan, Yanfang</creatorcontrib><creatorcontrib>Wu, Weili</creatorcontrib><creatorcontrib>Liu, Licheng</creatorcontrib><creatorcontrib>Zhang, Yuan</creatorcontrib><creatorcontrib>Huang, Xueyong</creatorcontrib><creatorcontrib>Wang, Pengzhi</creatorcontrib><creatorcontrib>Zhao, Jinyin</creatorcontrib><creatorcontrib>Kang, Xiaoping</creatorcontrib><creatorcontrib>Jiang, Hua</creatorcontrib><creatorcontrib>Cao, Yuanyin</creatorcontrib><creatorcontrib>Zheng, Yuling</creatorcontrib><creatorcontrib>Jiang, Yongqiang</creatorcontrib><creatorcontrib>Li, Yan</creatorcontrib><creatorcontrib>Yang, Yinhui</creatorcontrib><creatorcontrib>Chen, Weijun</creatorcontrib><title>Simultaneous detection and identification of enteric viruses by PCR-mass assay</title><title>PloS one</title><addtitle>PLoS One</addtitle><description>Simultaneous detection of enteric viruses that cause similar symptoms (e.g. hand, foot and mouth disease) is essential to the prevention of outbreaks and control of infections. 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In this study, a novel PCR-Mass assay combining multiplex polymerase chain reaction (PCR) with matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) was developed and used for simultaneous detection of eight distinct human enteric viruses. Enteric viral isolates and standard viral RNAs were examined to determine the sensitivity and specificity of the PCR-Mass assay. Clinical performance was evaluated with a total of 101 clinical specimens from patients suspected of having hand, foot and mouth disease (HFMD). The results were compared to those of previous analyses using real-time RT-PCR. The identification of specific viruses and clinical specimens shows that the PCR-Mass assay performed as well as or better than standard methods with respect to indicating the presence of multiplex pathogens in a single specimen.</abstract><cop>United States</cop><pub>Public Library of Science</pub><pmid>22870310</pmid><doi>10.1371/journal.pone.0042251</doi><tpages>e42251</tpages><oa>free_for_read</oa></addata></record> |
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| identifier | ISSN: 1932-6203 |
| ispartof | PloS one, 2012-08, Vol.7 (8), p.e42251-e42251 |
| issn | 1932-6203 1932-6203 |
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| source | Public Library of Science (PLoS) Journals Open Access; MEDLINE; PubMed Central(OpenAccess); Free E-Journal (出版社公開部分のみ）; DOAJ Directory of Open Access Journals; Free Full-Text Journals in Chemistry |
| subjects | Assaying Biology Coxsackievirus infections Desorption Disease control Echovirus Epidemics Female Foot and mouth disease Hand-foot-and-mouth disease Health aspects Human papillomavirus Humans Infection Infection control Ionization Ions Lasers Male Mass spectrometry Mass spectroscopy Medical research Medicine Multiplex Polymerase Chain Reaction - methods Multiplexing Outbreaks Polymerase chain reaction RNA Virus Infections - diagnosis RNA Virus Infections - genetics RNA Virus Infections - metabolism RNA Viruses - genetics RNA Viruses - metabolism RNA, Viral - genetics RNA, Viral - metabolism Sensitivity analysis Viral infections Viruses |
| title | Simultaneous detection and identification of enteric viruses by PCR-mass assay |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2024-12-25T06%3A32%3A35IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-gale_plos_&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Simultaneous%20detection%20and%20identification%20of%20enteric%20viruses%20by%20PCR-mass%20assay&rft.jtitle=PloS%20one&rft.au=Piao,%20Jingzi&rft.date=2012-08-01&rft.volume=7&rft.issue=8&rft.spage=e42251&rft.epage=e42251&rft.pages=e42251-e42251&rft.issn=1932-6203&rft.eissn=1932-6203&rft_id=info:doi/10.1371/journal.pone.0042251&rft_dat=%3Cgale_plos_%3EA543297030%3C/gale_plos_%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=1326222946&rft_id=info:pmid/22870310&rft_galeid=A543297030&rft_doaj_id=oai_doaj_org_article_e3f91faf28164b56ba8100714f3a2f73&rfr_iscdi=true |