High rates of detection of respiratory viruses in tonsillar tissues from children with chronic adenotonsillar disease
Chronic tonsillar diseases are an important health problem, leading to large numbers of surgical procedures worldwide. Little is known about pathogenesis of these diseases. In order to investigate the role of respiratory viruses in chronic adenotonsillar diseases, we developed a cross-sectional stud...
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| Veröffentlicht in: | PloS one 2012-08, Vol.7 (8), p.e42136-e42136 |
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| creator | Proenca-Modena, Jose Luiz Pereira Valera, Fabiana Cardoso Jacob, Marcos Gerhardinger Buzatto, Guilherme Pietrucci Saturno, Tamara Honorato Lopes, Lucia Souza, Jamila Mendonça Escremim Paula, Flavia Silva, Maria Lucia Carenzi, Lucas Rodrigues Tamashiro, Edwin Arruda, Eurico Anselmo-Lima, Wilma Terezinha |
| description | Chronic tonsillar diseases are an important health problem, leading to large numbers of surgical procedures worldwide. Little is known about pathogenesis of these diseases. In order to investigate the role of respiratory viruses in chronic adenotonsillar diseases, we developed a cross-sectional study to determine the rates of viral detections of common respiratory viruses detected by TaqMan real time PCR (qPCR) in nasopharyngeal secretions, tonsillar tissues and peripheral blood from 121 children with chronic tonsillar diseases, without symptoms of acute respiratory infections. At least one respiratory virus was detected in 97.5% of patients. The viral co-infection rate was 69.5%. The most frequently detected viruses were human adenovirus in 47.1%, human enterovirus in 40.5%, human rhinovirus in 38%, human bocavirus in 29.8%, human metapneumovirus in 17.4% and human respiratory syncytial virus in 15.7%. Results of qPCR varied widely between sample sites: human adenovirus, human bocavirus and human enterovirus were predominantly detected in tissues, while human rhinovirus was more frequently detected in secretions. Rates of virus detection were remarkably high in tonsil tissues: over 85% in adenoids and close to 70% in palatine tonsils. In addition, overall virus detection rates were higher in more hypertrophic than in smaller adenoids (p = 0.05), and in the particular case of human enteroviruses, they were detected more frequently (p = 0.05) in larger palatine tonsils than in smaller ones. While persistence/latency of DNA viruses in tonsillar tissues has been documented, such is not the case of RNA viruses. Respiratory viruses are highly prevalent in adenoids and palatine tonsils of patients with chronic tonsillar diseases, and persistence of these viruses in tonsils may stimulate chronic inflammation and play a role in the pathogenesis of these diseases. |
| doi_str_mv | 10.1371/journal.pone.0042136 |
| format | Article |
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Little is known about pathogenesis of these diseases. In order to investigate the role of respiratory viruses in chronic adenotonsillar diseases, we developed a cross-sectional study to determine the rates of viral detections of common respiratory viruses detected by TaqMan real time PCR (qPCR) in nasopharyngeal secretions, tonsillar tissues and peripheral blood from 121 children with chronic tonsillar diseases, without symptoms of acute respiratory infections. At least one respiratory virus was detected in 97.5% of patients. The viral co-infection rate was 69.5%. The most frequently detected viruses were human adenovirus in 47.1%, human enterovirus in 40.5%, human rhinovirus in 38%, human bocavirus in 29.8%, human metapneumovirus in 17.4% and human respiratory syncytial virus in 15.7%. Results of qPCR varied widely between sample sites: human adenovirus, human bocavirus and human enterovirus were predominantly detected in tissues, while human rhinovirus was more frequently detected in secretions. Rates of virus detection were remarkably high in tonsil tissues: over 85% in adenoids and close to 70% in palatine tonsils. In addition, overall virus detection rates were higher in more hypertrophic than in smaller adenoids (p = 0.05), and in the particular case of human enteroviruses, they were detected more frequently (p = 0.05) in larger palatine tonsils than in smaller ones. While persistence/latency of DNA viruses in tonsillar tissues has been documented, such is not the case of RNA viruses. Respiratory viruses are highly prevalent in adenoids and palatine tonsils of patients with chronic tonsillar diseases, and persistence of these viruses in tonsils may stimulate chronic inflammation and play a role in the pathogenesis of these diseases.</description><identifier>ISSN: 1932-6203</identifier><identifier>EISSN: 1932-6203</identifier><identifier>DOI: 10.1371/journal.pone.0042136</identifier><identifier>PMID: 22870291</identifier><language>eng</language><publisher>United States: Public Library of Science</publisher><subject>Adenoids - pathology ; Adenoids - virology ; Adenoviruses ; Adenoviruses, Human - genetics ; Adolescent ; Age ; Apoptosis ; Biology ; Child ; Child, Preschool ; Children ; Chronic Disease ; Chronic illnesses ; Deoxyribonucleic acid ; Disease ; Diseases ; DNA ; DNA viruses ; DNA, Viral - genetics ; Enteroviruses ; Eutrophication ; Female ; Health aspects ; Human bocavirus - genetics ; Humans ; Infant ; Infections ; Latency ; Male ; Medical research ; Medicine ; Otolaryngology ; Palatine Tonsil - pathology ; Palatine Tonsil - virology ; Pathogenesis ; Pathogens ; Patients ; Peripheral blood ; Real-Time Polymerase Chain Reaction ; Respiratory diseases ; Respiratory syncytial virus ; Rhinovirus ; Ribonucleic acid ; RNA ; RNA viruses ; RNA Viruses - genetics ; RNA, Viral - genetics ; Secretions ; Surgery ; Throat surgery ; Tissues ; Tonsil ; Tonsillitis ; Tonsillitis - epidemiology ; Tonsillitis - genetics ; Tonsillitis - pathology ; Tonsillitis - virology ; Viral infections ; Virology ; Virus Diseases - epidemiology ; Virus Diseases - genetics ; Virus Diseases - pathology ; Virus Diseases - virology ; Viruses</subject><ispartof>PloS one, 2012-08, Vol.7 (8), p.e42136-e42136</ispartof><rights>COPYRIGHT 2012 Public Library of Science</rights><rights>Proenca-Modena et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License: https://creativecommons.org/licenses/by/4.0/ (the “License”), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><rights>2012 Proenca-Modena et al 2012 Proenca-Modena et al</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c692t-84a0f0ad5d97696582fe3c6e9a991fac18186ffdb04da0ffa635d7429acd8c8d3</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC3411673/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC3411673/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,723,776,780,860,881,2096,2915,23845,27901,27902,53766,53768,79343,79344</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/22870291$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Proenca-Modena, Jose Luiz</creatorcontrib><creatorcontrib>Pereira Valera, Fabiana Cardoso</creatorcontrib><creatorcontrib>Jacob, Marcos Gerhardinger</creatorcontrib><creatorcontrib>Buzatto, Guilherme Pietrucci</creatorcontrib><creatorcontrib>Saturno, Tamara Honorato</creatorcontrib><creatorcontrib>Lopes, Lucia</creatorcontrib><creatorcontrib>Souza, Jamila Mendonça</creatorcontrib><creatorcontrib>Escremim Paula, Flavia</creatorcontrib><creatorcontrib>Silva, Maria Lucia</creatorcontrib><creatorcontrib>Carenzi, Lucas Rodrigues</creatorcontrib><creatorcontrib>Tamashiro, Edwin</creatorcontrib><creatorcontrib>Arruda, Eurico</creatorcontrib><creatorcontrib>Anselmo-Lima, Wilma Terezinha</creatorcontrib><title>High rates of detection of respiratory viruses in tonsillar tissues from children with chronic adenotonsillar disease</title><title>PloS one</title><addtitle>PLoS One</addtitle><description>Chronic tonsillar diseases are an important health problem, leading to large numbers of surgical procedures worldwide. Little is known about pathogenesis of these diseases. In order to investigate the role of respiratory viruses in chronic adenotonsillar diseases, we developed a cross-sectional study to determine the rates of viral detections of common respiratory viruses detected by TaqMan real time PCR (qPCR) in nasopharyngeal secretions, tonsillar tissues and peripheral blood from 121 children with chronic tonsillar diseases, without symptoms of acute respiratory infections. At least one respiratory virus was detected in 97.5% of patients. The viral co-infection rate was 69.5%. The most frequently detected viruses were human adenovirus in 47.1%, human enterovirus in 40.5%, human rhinovirus in 38%, human bocavirus in 29.8%, human metapneumovirus in 17.4% and human respiratory syncytial virus in 15.7%. Results of qPCR varied widely between sample sites: human adenovirus, human bocavirus and human enterovirus were predominantly detected in tissues, while human rhinovirus was more frequently detected in secretions. Rates of virus detection were remarkably high in tonsil tissues: over 85% in adenoids and close to 70% in palatine tonsils. In addition, overall virus detection rates were higher in more hypertrophic than in smaller adenoids (p = 0.05), and in the particular case of human enteroviruses, they were detected more frequently (p = 0.05) in larger palatine tonsils than in smaller ones. While persistence/latency of DNA viruses in tonsillar tissues has been documented, such is not the case of RNA viruses. Respiratory viruses are highly prevalent in adenoids and palatine tonsils of patients with chronic tonsillar diseases, and persistence of these viruses in tonsils may stimulate chronic inflammation and play a role in the pathogenesis of these diseases.</description><subject>Adenoids - pathology</subject><subject>Adenoids - virology</subject><subject>Adenoviruses</subject><subject>Adenoviruses, Human - genetics</subject><subject>Adolescent</subject><subject>Age</subject><subject>Apoptosis</subject><subject>Biology</subject><subject>Child</subject><subject>Child, Preschool</subject><subject>Children</subject><subject>Chronic Disease</subject><subject>Chronic illnesses</subject><subject>Deoxyribonucleic acid</subject><subject>Disease</subject><subject>Diseases</subject><subject>DNA</subject><subject>DNA viruses</subject><subject>DNA, Viral - genetics</subject><subject>Enteroviruses</subject><subject>Eutrophication</subject><subject>Female</subject><subject>Health aspects</subject><subject>Human bocavirus - genetics</subject><subject>Humans</subject><subject>Infant</subject><subject>Infections</subject><subject>Latency</subject><subject>Male</subject><subject>Medical research</subject><subject>Medicine</subject><subject>Otolaryngology</subject><subject>Palatine Tonsil - pathology</subject><subject>Palatine Tonsil - virology</subject><subject>Pathogenesis</subject><subject>Pathogens</subject><subject>Patients</subject><subject>Peripheral blood</subject><subject>Real-Time Polymerase Chain Reaction</subject><subject>Respiratory diseases</subject><subject>Respiratory syncytial virus</subject><subject>Rhinovirus</subject><subject>Ribonucleic acid</subject><subject>RNA</subject><subject>RNA viruses</subject><subject>RNA Viruses - genetics</subject><subject>RNA, Viral - genetics</subject><subject>Secretions</subject><subject>Surgery</subject><subject>Throat surgery</subject><subject>Tissues</subject><subject>Tonsil</subject><subject>Tonsillitis</subject><subject>Tonsillitis - epidemiology</subject><subject>Tonsillitis - genetics</subject><subject>Tonsillitis - pathology</subject><subject>Tonsillitis - virology</subject><subject>Viral infections</subject><subject>Virology</subject><subject>Virus Diseases - epidemiology</subject><subject>Virus Diseases - genetics</subject><subject>Virus Diseases - pathology</subject><subject>Virus Diseases - virology</subject><subject>Viruses</subject><issn>1932-6203</issn><issn>1932-6203</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2012</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>BENPR</sourceid><sourceid>DOA</sourceid><recordid>eNqNk0tv1DAUhSMEoqXwDxBEQkKwmMGvOPEGqaqAjlSpEq-tdcePGVcZe7CdQv89HiYtE9QFyiLx9XeO7RPfqnqO0RzTFr-7CkP00M-3wZs5Qoxgyh9Ux1hQMuME0YcH30fVk5SuEGpox_nj6oiQrkVE4ONqOHerdR0hm1QHW2uTjcou-N0gmrR1ZSrEm_raxSEVxvk6B59c30Oss0tpKEUbw6ZWa9fraHz90-V1GcXgnapBGx_-KrRLBpJ5Wj2y0CfzbHyfVN8-fvh6dj67uPy0ODu9mCkuSJ51DJBFoBstWi540xFrqOJGgBDYgsId7ri1eomYLqQFThvdMiJA6U51mp5UL_e-2z4kOSaWJKaEE0J40xZisSd0gCu5jW4D8UYGcPJPIcSVhJid6o2kBW-tMFQ3wEjHBLR4ybDVCtpWKFa83o-rDcuN0cr4HKGfmE5nvFvLVbiWlGHMW1oM3owGMfwowWa5cUmZkpw3YSj7RpS0lBOKCvrqH_T-043UCsoBnLehrKt2pvK0YbT4MM4LNb-HKo82G6fK9bKu1CeCtxNBYbL5lVcwpCQXXz7_P3v5fcq-PmDXBvq8TqEfdhcyTUG2B1UMKUVj70LGSO664zYNuesOOXZHkb04_EF3ott2oL8BghEMZA</recordid><startdate>20120803</startdate><enddate>20120803</enddate><creator>Proenca-Modena, Jose Luiz</creator><creator>Pereira Valera, Fabiana Cardoso</creator><creator>Jacob, Marcos Gerhardinger</creator><creator>Buzatto, Guilherme Pietrucci</creator><creator>Saturno, Tamara Honorato</creator><creator>Lopes, Lucia</creator><creator>Souza, Jamila Mendonça</creator><creator>Escremim Paula, Flavia</creator><creator>Silva, Maria Lucia</creator><creator>Carenzi, Lucas Rodrigues</creator><creator>Tamashiro, Edwin</creator><creator>Arruda, Eurico</creator><creator>Anselmo-Lima, Wilma Terezinha</creator><general>Public Library of Science</general><general>Public Library of Science (PLoS)</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>IOV</scope><scope>ISR</scope><scope>3V.</scope><scope>7QG</scope><scope>7QL</scope><scope>7QO</scope><scope>7RV</scope><scope>7SN</scope><scope>7SS</scope><scope>7T5</scope><scope>7TG</scope><scope>7TM</scope><scope>7U9</scope><scope>7X2</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>8AO</scope><scope>8C1</scope><scope>8FD</scope><scope>8FE</scope><scope>8FG</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABJCF</scope><scope>ABUWG</scope><scope>AEUYN</scope><scope>AFKRA</scope><scope>ARAPS</scope><scope>ATCPS</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BGLVJ</scope><scope>BHPHI</scope><scope>C1K</scope><scope>CCPQU</scope><scope>COVID</scope><scope>D1I</scope><scope>DWQXO</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>H94</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>KB.</scope><scope>KB0</scope><scope>KL.</scope><scope>L6V</scope><scope>LK8</scope><scope>M0K</scope><scope>M0S</scope><scope>M1P</scope><scope>M7N</scope><scope>M7P</scope><scope>M7S</scope><scope>NAPCQ</scope><scope>P5Z</scope><scope>P62</scope><scope>P64</scope><scope>PATMY</scope><scope>PDBOC</scope><scope>PIMPY</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>PTHSS</scope><scope>PYCSY</scope><scope>RC3</scope><scope>7X8</scope><scope>5PM</scope><scope>DOA</scope></search><sort><creationdate>20120803</creationdate><title>High rates of detection of respiratory viruses in tonsillar tissues from children with chronic adenotonsillar disease</title><author>Proenca-Modena, Jose Luiz ; Pereira Valera, Fabiana Cardoso ; Jacob, Marcos Gerhardinger ; Buzatto, Guilherme Pietrucci ; Saturno, Tamara Honorato ; Lopes, Lucia ; Souza, Jamila Mendonça ; Escremim Paula, Flavia ; Silva, Maria Lucia ; Carenzi, Lucas Rodrigues ; Tamashiro, Edwin ; Arruda, Eurico ; Anselmo-Lima, Wilma Terezinha</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c692t-84a0f0ad5d97696582fe3c6e9a991fac18186ffdb04da0ffa635d7429acd8c8d3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2012</creationdate><topic>Adenoids - 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genetics</topic><topic>RNA, Viral - genetics</topic><topic>Secretions</topic><topic>Surgery</topic><topic>Throat surgery</topic><topic>Tissues</topic><topic>Tonsil</topic><topic>Tonsillitis</topic><topic>Tonsillitis - epidemiology</topic><topic>Tonsillitis - genetics</topic><topic>Tonsillitis - pathology</topic><topic>Tonsillitis - virology</topic><topic>Viral infections</topic><topic>Virology</topic><topic>Virus Diseases - epidemiology</topic><topic>Virus Diseases - genetics</topic><topic>Virus Diseases - pathology</topic><topic>Virus Diseases - virology</topic><topic>Viruses</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Proenca-Modena, Jose Luiz</creatorcontrib><creatorcontrib>Pereira Valera, Fabiana Cardoso</creatorcontrib><creatorcontrib>Jacob, Marcos Gerhardinger</creatorcontrib><creatorcontrib>Buzatto, Guilherme Pietrucci</creatorcontrib><creatorcontrib>Saturno, Tamara Honorato</creatorcontrib><creatorcontrib>Lopes, Lucia</creatorcontrib><creatorcontrib>Souza, Jamila Mendonça</creatorcontrib><creatorcontrib>Escremim Paula, Flavia</creatorcontrib><creatorcontrib>Silva, Maria Lucia</creatorcontrib><creatorcontrib>Carenzi, Lucas Rodrigues</creatorcontrib><creatorcontrib>Tamashiro, Edwin</creatorcontrib><creatorcontrib>Arruda, Eurico</creatorcontrib><creatorcontrib>Anselmo-Lima, Wilma Terezinha</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Gale In Context: Opposing Viewpoints</collection><collection>Gale In Context: Science</collection><collection>ProQuest Central (Corporate)</collection><collection>Animal Behavior Abstracts</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Biotechnology Research Abstracts</collection><collection>Nursing & Allied Health Database</collection><collection>Ecology Abstracts</collection><collection>Entomology Abstracts (Full archive)</collection><collection>Immunology Abstracts</collection><collection>Meteorological & Geoastrophysical Abstracts</collection><collection>Nucleic Acids Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>Agricultural Science Collection</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Medical Database (Alumni Edition)</collection><collection>ProQuest Pharma Collection</collection><collection>Public Health Database</collection><collection>Technology Research Database</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Technology Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>Materials Science & Engineering Collection</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest One Sustainability</collection><collection>ProQuest Central UK/Ireland</collection><collection>Advanced Technologies & Aerospace Collection</collection><collection>Agricultural & Environmental Science Collection</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Technology Collection</collection><collection>Natural Science Collection</collection><collection>Environmental Sciences and Pollution Management</collection><collection>ProQuest One Community College</collection><collection>Coronavirus Research Database</collection><collection>ProQuest Materials Science Collection</collection><collection>ProQuest Central Korea</collection><collection>Engineering Research Database</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Materials Science Database</collection><collection>Nursing & Allied Health Database (Alumni Edition)</collection><collection>Meteorological & Geoastrophysical Abstracts - 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Academic</collection><collection>PubMed Central (Full Participant titles)</collection><collection>DOAJ Directory of Open Access Journals</collection><jtitle>PloS one</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Proenca-Modena, Jose Luiz</au><au>Pereira Valera, Fabiana Cardoso</au><au>Jacob, Marcos Gerhardinger</au><au>Buzatto, Guilherme Pietrucci</au><au>Saturno, Tamara Honorato</au><au>Lopes, Lucia</au><au>Souza, Jamila Mendonça</au><au>Escremim Paula, Flavia</au><au>Silva, Maria Lucia</au><au>Carenzi, Lucas Rodrigues</au><au>Tamashiro, Edwin</au><au>Arruda, Eurico</au><au>Anselmo-Lima, Wilma Terezinha</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>High rates of detection of respiratory viruses in tonsillar tissues from children with chronic adenotonsillar disease</atitle><jtitle>PloS one</jtitle><addtitle>PLoS One</addtitle><date>2012-08-03</date><risdate>2012</risdate><volume>7</volume><issue>8</issue><spage>e42136</spage><epage>e42136</epage><pages>e42136-e42136</pages><issn>1932-6203</issn><eissn>1932-6203</eissn><abstract>Chronic tonsillar diseases are an important health problem, leading to large numbers of surgical procedures worldwide. Little is known about pathogenesis of these diseases. In order to investigate the role of respiratory viruses in chronic adenotonsillar diseases, we developed a cross-sectional study to determine the rates of viral detections of common respiratory viruses detected by TaqMan real time PCR (qPCR) in nasopharyngeal secretions, tonsillar tissues and peripheral blood from 121 children with chronic tonsillar diseases, without symptoms of acute respiratory infections. At least one respiratory virus was detected in 97.5% of patients. The viral co-infection rate was 69.5%. The most frequently detected viruses were human adenovirus in 47.1%, human enterovirus in 40.5%, human rhinovirus in 38%, human bocavirus in 29.8%, human metapneumovirus in 17.4% and human respiratory syncytial virus in 15.7%. Results of qPCR varied widely between sample sites: human adenovirus, human bocavirus and human enterovirus were predominantly detected in tissues, while human rhinovirus was more frequently detected in secretions. Rates of virus detection were remarkably high in tonsil tissues: over 85% in adenoids and close to 70% in palatine tonsils. In addition, overall virus detection rates were higher in more hypertrophic than in smaller adenoids (p = 0.05), and in the particular case of human enteroviruses, they were detected more frequently (p = 0.05) in larger palatine tonsils than in smaller ones. While persistence/latency of DNA viruses in tonsillar tissues has been documented, such is not the case of RNA viruses. Respiratory viruses are highly prevalent in adenoids and palatine tonsils of patients with chronic tonsillar diseases, and persistence of these viruses in tonsils may stimulate chronic inflammation and play a role in the pathogenesis of these diseases.</abstract><cop>United States</cop><pub>Public Library of Science</pub><pmid>22870291</pmid><doi>10.1371/journal.pone.0042136</doi><tpages>e42136</tpages><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 1932-6203 |
| ispartof | PloS one, 2012-08, Vol.7 (8), p.e42136-e42136 |
| issn | 1932-6203 1932-6203 |
| language | eng |
| recordid | cdi_plos_journals_1326222657 |
| source | MEDLINE; DOAJ Directory of Open Access Journals; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; PubMed Central; Free Full-Text Journals in Chemistry; Public Library of Science (PLoS) |
| subjects | Adenoids - pathology Adenoids - virology Adenoviruses Adenoviruses, Human - genetics Adolescent Age Apoptosis Biology Child Child, Preschool Children Chronic Disease Chronic illnesses Deoxyribonucleic acid Disease Diseases DNA DNA viruses DNA, Viral - genetics Enteroviruses Eutrophication Female Health aspects Human bocavirus - genetics Humans Infant Infections Latency Male Medical research Medicine Otolaryngology Palatine Tonsil - pathology Palatine Tonsil - virology Pathogenesis Pathogens Patients Peripheral blood Real-Time Polymerase Chain Reaction Respiratory diseases Respiratory syncytial virus Rhinovirus Ribonucleic acid RNA RNA viruses RNA Viruses - genetics RNA, Viral - genetics Secretions Surgery Throat surgery Tissues Tonsil Tonsillitis Tonsillitis - epidemiology Tonsillitis - genetics Tonsillitis - pathology Tonsillitis - virology Viral infections Virology Virus Diseases - epidemiology Virus Diseases - genetics Virus Diseases - pathology Virus Diseases - virology Viruses |
| title | High rates of detection of respiratory viruses in tonsillar tissues from children with chronic adenotonsillar disease |
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