DNA polymorphisms and biocontrol of Bacillus antagonistic to citrus bacterial canker with indication of the interference of phyllosphere biofilms
Citrus bacterial canker caused by Xanthomonas axonopodis pv. citri is a devastating disease resulting in significant crop losses in various citrus cultivars worldwide. A biocontrol agent has not been recommended for this disease. To explore the potential of bacilli native to Taiwan to control this d...
Gespeichert in:
| Veröffentlicht in: | PloS one 2012-07, Vol.7 (7), p.e42124-e42124 |
|---|---|
| Hauptverfasser: | , , , , , , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | e42124 |
|---|---|
| container_issue | 7 |
| container_start_page | e42124 |
| container_title | PloS one |
| container_volume | 7 |
| creator | Huang, Tzu-Pi Tzeng, Dean Der-Syh Wong, Amy C L Chen, Chun-Han Lu, Kuan-Min Lee, Ya-Huei Huang, Wen-Di Hwang, Bing-Fang Tzeng, Kuo-Ching |
| description | Citrus bacterial canker caused by Xanthomonas axonopodis pv. citri is a devastating disease resulting in significant crop losses in various citrus cultivars worldwide. A biocontrol agent has not been recommended for this disease. To explore the potential of bacilli native to Taiwan to control this disease, Bacillus species with a broad spectrum of antagonistic activity against various phytopathogens were isolated from plant potting mixes, organic compost and the rhizosphere soil. Seven strains TKS1-1, OF3-16, SP4-17, HSP1, WG6-14, TLB7-7, and WP8-12 showing superior antagonistic activity were chosen for biopesticide development. The genetic identity based on 16S rDNA sequences indicated that all seven native strains were close relatives of the B. subtilis group and appeared to be discrete from the B. cereus group. DNA polymorphisms in strains WG6-14, SP4-17, TKS1-1, and WP8-12, as revealed by repetitive sequence-based PCR with the BOXA1R primers were similar to each other, but different from those of the respective Bacillus type strains. However, molecular typing of the strains using either tDNA-intergenic spacer regions or 16S-23S intergenic transcribed spacer regions was unable to differentiate the strains at the species level. Strains TKS1-1 and WG6-14 attenuated symptom development of citrus bacterial canker, which was found to be correlated with a reduction in colonization and biofilm formation by X. axonopodis pv. citri on leaf surfaces. The application of a Bacillus strain TKS1-1 endospore formulation to the leaf surfaces of citrus reduced the incidence of citrus bacterial canker and could prevent development of the disease. |
| doi_str_mv | 10.1371/journal.pone.0042124 |
| format | Article |
| fullrecord | <record><control><sourceid>gale_plos_</sourceid><recordid>TN_cdi_plos_journals_1325527253</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><galeid>A498244922</galeid><doaj_id>oai_doaj_org_article_bdf6fbc328f9426086da8e0a73bc29d1</doaj_id><sourcerecordid>A498244922</sourcerecordid><originalsourceid>FETCH-LOGICAL-c692t-66b13d35e7572c06a011e1cc642456965f6f4c88df87a823aa7c6d1cc9b40aa03</originalsourceid><addsrcrecordid>eNqNk9uO0zAQhiMEYpeFN0AQCQnBRYtPcZIbpLKcKq1YidOtNXGcxsWJg-0AfQzeGIdmVy3aC-QLW-Nv_hmPZ5LkIUZLTHP8YmtH14NZDrZXS4QYwYTdSk5xScmCE0RvH5xPknvebxHKaMH53eSEkIIVOSlOk9-vP6zSwZpdZ93Qat_5FPo6rbSVtg_OmtQ26SuQ2phxugqwsb32Qcs02FTq4KK5AhmU02BSCf035dKfOrSp7mstIWjbTxqhVdESsUY51Us12YZ2Z4z1QxtNU8hGm87fT-40YLx6MO9nyZe3bz6fv19cXL5bn68uFpKXJCw4rzCtaabyLCcScUAYKywlZ4RlvORZwxsmi6JuihwKQgFyyesIlBVDAIieJY_3ukNMQczV9AJTkmUkJxmNxHpP1Ba2YnC6A7cTFrT4a7BuI8DFShglqjqGqyQlRVMywlHBaygUgpxWkpQ1jlov52hj1alaqlhcMEeixze9bsXG_hCUoRznU7rPZgFnv4_KB9FpL5Ux0Cs7xrwRRRliPCcRffIPevPrZmoD8QG6b2yMKydRsWJlQRgryaS1vIGKq1adji2i4p-pY4fnRw5TG6lfYQOj92L96eP_s5dfj9mnB2yrwITWWzNO_eWPQbYHpbPeO9VcFxkjMU3OVTXENDlinpzo9ujwg66drkaF_gEEKxZU</addsrcrecordid><sourcetype>Open Website</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>1325527253</pqid></control><display><type>article</type><title>DNA polymorphisms and biocontrol of Bacillus antagonistic to citrus bacterial canker with indication of the interference of phyllosphere biofilms</title><source>MEDLINE</source><source>DOAJ Directory of Open Access Journals</source><source>Public Library of Science (PLoS) Journals Open Access</source><source>EZB-FREE-00999 freely available EZB journals</source><source>PubMed Central</source><source>Free Full-Text Journals in Chemistry</source><creator>Huang, Tzu-Pi ; Tzeng, Dean Der-Syh ; Wong, Amy C L ; Chen, Chun-Han ; Lu, Kuan-Min ; Lee, Ya-Huei ; Huang, Wen-Di ; Hwang, Bing-Fang ; Tzeng, Kuo-Ching</creator><contributor>Driks, Adam</contributor><creatorcontrib>Huang, Tzu-Pi ; Tzeng, Dean Der-Syh ; Wong, Amy C L ; Chen, Chun-Han ; Lu, Kuan-Min ; Lee, Ya-Huei ; Huang, Wen-Di ; Hwang, Bing-Fang ; Tzeng, Kuo-Ching ; Driks, Adam</creatorcontrib><description>Citrus bacterial canker caused by Xanthomonas axonopodis pv. citri is a devastating disease resulting in significant crop losses in various citrus cultivars worldwide. A biocontrol agent has not been recommended for this disease. To explore the potential of bacilli native to Taiwan to control this disease, Bacillus species with a broad spectrum of antagonistic activity against various phytopathogens were isolated from plant potting mixes, organic compost and the rhizosphere soil. Seven strains TKS1-1, OF3-16, SP4-17, HSP1, WG6-14, TLB7-7, and WP8-12 showing superior antagonistic activity were chosen for biopesticide development. The genetic identity based on 16S rDNA sequences indicated that all seven native strains were close relatives of the B. subtilis group and appeared to be discrete from the B. cereus group. DNA polymorphisms in strains WG6-14, SP4-17, TKS1-1, and WP8-12, as revealed by repetitive sequence-based PCR with the BOXA1R primers were similar to each other, but different from those of the respective Bacillus type strains. However, molecular typing of the strains using either tDNA-intergenic spacer regions or 16S-23S intergenic transcribed spacer regions was unable to differentiate the strains at the species level. Strains TKS1-1 and WG6-14 attenuated symptom development of citrus bacterial canker, which was found to be correlated with a reduction in colonization and biofilm formation by X. axonopodis pv. citri on leaf surfaces. The application of a Bacillus strain TKS1-1 endospore formulation to the leaf surfaces of citrus reduced the incidence of citrus bacterial canker and could prevent development of the disease.</description><identifier>ISSN: 1932-6203</identifier><identifier>EISSN: 1932-6203</identifier><identifier>DOI: 10.1371/journal.pone.0042124</identifier><identifier>PMID: 22848728</identifier><language>eng</language><publisher>United States: Public Library of Science</publisher><subject>Agriculture ; Antibiotics ; Bacilli ; Bacillus - classification ; Bacillus - genetics ; Bacillus - physiology ; Bacillus cereus ; Bacillus subtilis ; Bacteria ; Biofilms ; Biofilms - growth & development ; Biological control ; Biological Control Agents ; Biological pest control ; Biology ; Canker ; Citrus - microbiology ; Citrus fruits ; Cloning ; Cluster Analysis ; Colonization ; Composts ; Cultivars ; Deoxyribonucleic acid ; Disease control ; DNA ; DNA, Bacterial - genetics ; DNA, Intergenic - genetics ; Gene sequencing ; Genetic aspects ; Genetic polymorphisms ; Laboratories ; Leaves ; Molecular Sequence Data ; Pathogens ; Phyllosphere ; Phylogeny ; Plant diseases ; Plant Diseases - microbiology ; Plant Leaves - microbiology ; Plant pathology ; Polymorphism, Genetic ; Pseudomonas syringae ; Repetitive Sequences, Nucleic Acid - genetics ; Rhizoctonia solani ; Rhizosphere ; RNA, Ribosomal, 16S - genetics ; rRNA 16S ; Sequence Analysis, RNA ; Soil Microbiology ; Strains (organisms) ; Xanthomonas axonopodis ; Xanthomonas axonopodis - physiology</subject><ispartof>PloS one, 2012-07, Vol.7 (7), p.e42124-e42124</ispartof><rights>COPYRIGHT 2012 Public Library of Science</rights><rights>Huang et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License: https://creativecommons.org/licenses/by/4.0/ (the “License”), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><rights>2012 Huang et al 2012 Huang et al</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c692t-66b13d35e7572c06a011e1cc642456965f6f4c88df87a823aa7c6d1cc9b40aa03</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC3407170/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC3407170/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,727,780,784,864,885,2102,2928,23866,27924,27925,53791,53793</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/22848728$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><contributor>Driks, Adam</contributor><creatorcontrib>Huang, Tzu-Pi</creatorcontrib><creatorcontrib>Tzeng, Dean Der-Syh</creatorcontrib><creatorcontrib>Wong, Amy C L</creatorcontrib><creatorcontrib>Chen, Chun-Han</creatorcontrib><creatorcontrib>Lu, Kuan-Min</creatorcontrib><creatorcontrib>Lee, Ya-Huei</creatorcontrib><creatorcontrib>Huang, Wen-Di</creatorcontrib><creatorcontrib>Hwang, Bing-Fang</creatorcontrib><creatorcontrib>Tzeng, Kuo-Ching</creatorcontrib><title>DNA polymorphisms and biocontrol of Bacillus antagonistic to citrus bacterial canker with indication of the interference of phyllosphere biofilms</title><title>PloS one</title><addtitle>PLoS One</addtitle><description>Citrus bacterial canker caused by Xanthomonas axonopodis pv. citri is a devastating disease resulting in significant crop losses in various citrus cultivars worldwide. A biocontrol agent has not been recommended for this disease. To explore the potential of bacilli native to Taiwan to control this disease, Bacillus species with a broad spectrum of antagonistic activity against various phytopathogens were isolated from plant potting mixes, organic compost and the rhizosphere soil. Seven strains TKS1-1, OF3-16, SP4-17, HSP1, WG6-14, TLB7-7, and WP8-12 showing superior antagonistic activity were chosen for biopesticide development. The genetic identity based on 16S rDNA sequences indicated that all seven native strains were close relatives of the B. subtilis group and appeared to be discrete from the B. cereus group. DNA polymorphisms in strains WG6-14, SP4-17, TKS1-1, and WP8-12, as revealed by repetitive sequence-based PCR with the BOXA1R primers were similar to each other, but different from those of the respective Bacillus type strains. However, molecular typing of the strains using either tDNA-intergenic spacer regions or 16S-23S intergenic transcribed spacer regions was unable to differentiate the strains at the species level. Strains TKS1-1 and WG6-14 attenuated symptom development of citrus bacterial canker, which was found to be correlated with a reduction in colonization and biofilm formation by X. axonopodis pv. citri on leaf surfaces. The application of a Bacillus strain TKS1-1 endospore formulation to the leaf surfaces of citrus reduced the incidence of citrus bacterial canker and could prevent development of the disease.</description><subject>Agriculture</subject><subject>Antibiotics</subject><subject>Bacilli</subject><subject>Bacillus - classification</subject><subject>Bacillus - genetics</subject><subject>Bacillus - physiology</subject><subject>Bacillus cereus</subject><subject>Bacillus subtilis</subject><subject>Bacteria</subject><subject>Biofilms</subject><subject>Biofilms - growth & development</subject><subject>Biological control</subject><subject>Biological Control Agents</subject><subject>Biological pest control</subject><subject>Biology</subject><subject>Canker</subject><subject>Citrus - microbiology</subject><subject>Citrus fruits</subject><subject>Cloning</subject><subject>Cluster Analysis</subject><subject>Colonization</subject><subject>Composts</subject><subject>Cultivars</subject><subject>Deoxyribonucleic acid</subject><subject>Disease control</subject><subject>DNA</subject><subject>DNA, Bacterial - genetics</subject><subject>DNA, Intergenic - genetics</subject><subject>Gene sequencing</subject><subject>Genetic aspects</subject><subject>Genetic polymorphisms</subject><subject>Laboratories</subject><subject>Leaves</subject><subject>Molecular Sequence Data</subject><subject>Pathogens</subject><subject>Phyllosphere</subject><subject>Phylogeny</subject><subject>Plant diseases</subject><subject>Plant Diseases - microbiology</subject><subject>Plant Leaves - microbiology</subject><subject>Plant pathology</subject><subject>Polymorphism, Genetic</subject><subject>Pseudomonas syringae</subject><subject>Repetitive Sequences, Nucleic Acid - genetics</subject><subject>Rhizoctonia solani</subject><subject>Rhizosphere</subject><subject>RNA, Ribosomal, 16S - genetics</subject><subject>rRNA 16S</subject><subject>Sequence Analysis, RNA</subject><subject>Soil Microbiology</subject><subject>Strains (organisms)</subject><subject>Xanthomonas axonopodis</subject><subject>Xanthomonas axonopodis - physiology</subject><issn>1932-6203</issn><issn>1932-6203</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2012</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><sourceid>DOA</sourceid><recordid>eNqNk9uO0zAQhiMEYpeFN0AQCQnBRYtPcZIbpLKcKq1YidOtNXGcxsWJg-0AfQzeGIdmVy3aC-QLW-Nv_hmPZ5LkIUZLTHP8YmtH14NZDrZXS4QYwYTdSk5xScmCE0RvH5xPknvebxHKaMH53eSEkIIVOSlOk9-vP6zSwZpdZ93Qat_5FPo6rbSVtg_OmtQ26SuQ2phxugqwsb32Qcs02FTq4KK5AhmU02BSCf035dKfOrSp7mstIWjbTxqhVdESsUY51Us12YZ2Z4z1QxtNU8hGm87fT-40YLx6MO9nyZe3bz6fv19cXL5bn68uFpKXJCw4rzCtaabyLCcScUAYKywlZ4RlvORZwxsmi6JuihwKQgFyyesIlBVDAIieJY_3ukNMQczV9AJTkmUkJxmNxHpP1Ba2YnC6A7cTFrT4a7BuI8DFShglqjqGqyQlRVMywlHBaygUgpxWkpQ1jlov52hj1alaqlhcMEeixze9bsXG_hCUoRznU7rPZgFnv4_KB9FpL5Ux0Cs7xrwRRRliPCcRffIPevPrZmoD8QG6b2yMKydRsWJlQRgryaS1vIGKq1adji2i4p-pY4fnRw5TG6lfYQOj92L96eP_s5dfj9mnB2yrwITWWzNO_eWPQbYHpbPeO9VcFxkjMU3OVTXENDlinpzo9ujwg66drkaF_gEEKxZU</recordid><startdate>20120727</startdate><enddate>20120727</enddate><creator>Huang, Tzu-Pi</creator><creator>Tzeng, Dean Der-Syh</creator><creator>Wong, Amy C L</creator><creator>Chen, Chun-Han</creator><creator>Lu, Kuan-Min</creator><creator>Lee, Ya-Huei</creator><creator>Huang, Wen-Di</creator><creator>Hwang, Bing-Fang</creator><creator>Tzeng, Kuo-Ching</creator><general>Public Library of Science</general><general>Public Library of Science (PLoS)</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>IOV</scope><scope>ISR</scope><scope>3V.</scope><scope>7QG</scope><scope>7QL</scope><scope>7QO</scope><scope>7RV</scope><scope>7SN</scope><scope>7SS</scope><scope>7T5</scope><scope>7TG</scope><scope>7TM</scope><scope>7U9</scope><scope>7X2</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>8AO</scope><scope>8C1</scope><scope>8FD</scope><scope>8FE</scope><scope>8FG</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABJCF</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>ARAPS</scope><scope>ATCPS</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BGLVJ</scope><scope>BHPHI</scope><scope>C1K</scope><scope>CCPQU</scope><scope>D1I</scope><scope>DWQXO</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>H94</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>KB.</scope><scope>KB0</scope><scope>KL.</scope><scope>L6V</scope><scope>LK8</scope><scope>M0K</scope><scope>M0S</scope><scope>M1P</scope><scope>M7N</scope><scope>M7P</scope><scope>M7S</scope><scope>NAPCQ</scope><scope>P5Z</scope><scope>P62</scope><scope>P64</scope><scope>PATMY</scope><scope>PDBOC</scope><scope>PIMPY</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PTHSS</scope><scope>PYCSY</scope><scope>RC3</scope><scope>7X8</scope><scope>5PM</scope><scope>DOA</scope></search><sort><creationdate>20120727</creationdate><title>DNA polymorphisms and biocontrol of Bacillus antagonistic to citrus bacterial canker with indication of the interference of phyllosphere biofilms</title><author>Huang, Tzu-Pi ; Tzeng, Dean Der-Syh ; Wong, Amy C L ; Chen, Chun-Han ; Lu, Kuan-Min ; Lee, Ya-Huei ; Huang, Wen-Di ; Hwang, Bing-Fang ; Tzeng, Kuo-Ching</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c692t-66b13d35e7572c06a011e1cc642456965f6f4c88df87a823aa7c6d1cc9b40aa03</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2012</creationdate><topic>Agriculture</topic><topic>Antibiotics</topic><topic>Bacilli</topic><topic>Bacillus - classification</topic><topic>Bacillus - genetics</topic><topic>Bacillus - physiology</topic><topic>Bacillus cereus</topic><topic>Bacillus subtilis</topic><topic>Bacteria</topic><topic>Biofilms</topic><topic>Biofilms - growth & development</topic><topic>Biological control</topic><topic>Biological Control Agents</topic><topic>Biological pest control</topic><topic>Biology</topic><topic>Canker</topic><topic>Citrus - microbiology</topic><topic>Citrus fruits</topic><topic>Cloning</topic><topic>Cluster Analysis</topic><topic>Colonization</topic><topic>Composts</topic><topic>Cultivars</topic><topic>Deoxyribonucleic acid</topic><topic>Disease control</topic><topic>DNA</topic><topic>DNA, Bacterial - genetics</topic><topic>DNA, Intergenic - genetics</topic><topic>Gene sequencing</topic><topic>Genetic aspects</topic><topic>Genetic polymorphisms</topic><topic>Laboratories</topic><topic>Leaves</topic><topic>Molecular Sequence Data</topic><topic>Pathogens</topic><topic>Phyllosphere</topic><topic>Phylogeny</topic><topic>Plant diseases</topic><topic>Plant Diseases - microbiology</topic><topic>Plant Leaves - microbiology</topic><topic>Plant pathology</topic><topic>Polymorphism, Genetic</topic><topic>Pseudomonas syringae</topic><topic>Repetitive Sequences, Nucleic Acid - genetics</topic><topic>Rhizoctonia solani</topic><topic>Rhizosphere</topic><topic>RNA, Ribosomal, 16S - genetics</topic><topic>rRNA 16S</topic><topic>Sequence Analysis, RNA</topic><topic>Soil Microbiology</topic><topic>Strains (organisms)</topic><topic>Xanthomonas axonopodis</topic><topic>Xanthomonas axonopodis - physiology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Huang, Tzu-Pi</creatorcontrib><creatorcontrib>Tzeng, Dean Der-Syh</creatorcontrib><creatorcontrib>Wong, Amy C L</creatorcontrib><creatorcontrib>Chen, Chun-Han</creatorcontrib><creatorcontrib>Lu, Kuan-Min</creatorcontrib><creatorcontrib>Lee, Ya-Huei</creatorcontrib><creatorcontrib>Huang, Wen-Di</creatorcontrib><creatorcontrib>Hwang, Bing-Fang</creatorcontrib><creatorcontrib>Tzeng, Kuo-Ching</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Gale In Context: Opposing Viewpoints</collection><collection>Gale In Context: Science</collection><collection>ProQuest Central (Corporate)</collection><collection>Animal Behavior Abstracts</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Biotechnology Research Abstracts</collection><collection>Nursing & Allied Health Database</collection><collection>Ecology Abstracts</collection><collection>Entomology Abstracts (Full archive)</collection><collection>Immunology Abstracts</collection><collection>Meteorological & Geoastrophysical Abstracts</collection><collection>Nucleic Acids Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>Agricultural Science Collection</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Medical Database (Alumni Edition)</collection><collection>ProQuest Pharma Collection</collection><collection>Public Health Database</collection><collection>Technology Research Database</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Technology Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>Materials Science & Engineering Collection</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest Central UK/Ireland</collection><collection>Advanced Technologies & Aerospace Collection</collection><collection>Agricultural & Environmental Science Collection</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Technology Collection</collection><collection>Natural Science Collection</collection><collection>Environmental Sciences and Pollution Management</collection><collection>ProQuest One Community College</collection><collection>ProQuest Materials Science Collection</collection><collection>ProQuest Central Korea</collection><collection>Engineering Research Database</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Materials Science Database</collection><collection>Nursing & Allied Health Database (Alumni Edition)</collection><collection>Meteorological & Geoastrophysical Abstracts - Academic</collection><collection>ProQuest Engineering Collection</collection><collection>ProQuest Biological Science Collection</collection><collection>Agricultural Science Database</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biological Science Database</collection><collection>Engineering Database</collection><collection>Nursing & Allied Health Premium</collection><collection>Advanced Technologies & Aerospace Database</collection><collection>ProQuest Advanced Technologies & Aerospace Collection</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Environmental Science Database</collection><collection>Materials Science Collection</collection><collection>Access via ProQuest (Open Access)</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>Engineering Collection</collection><collection>Environmental Science Collection</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><collection>DOAJ Directory of Open Access Journals</collection><jtitle>PloS one</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Huang, Tzu-Pi</au><au>Tzeng, Dean Der-Syh</au><au>Wong, Amy C L</au><au>Chen, Chun-Han</au><au>Lu, Kuan-Min</au><au>Lee, Ya-Huei</au><au>Huang, Wen-Di</au><au>Hwang, Bing-Fang</au><au>Tzeng, Kuo-Ching</au><au>Driks, Adam</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>DNA polymorphisms and biocontrol of Bacillus antagonistic to citrus bacterial canker with indication of the interference of phyllosphere biofilms</atitle><jtitle>PloS one</jtitle><addtitle>PLoS One</addtitle><date>2012-07-27</date><risdate>2012</risdate><volume>7</volume><issue>7</issue><spage>e42124</spage><epage>e42124</epage><pages>e42124-e42124</pages><issn>1932-6203</issn><eissn>1932-6203</eissn><abstract>Citrus bacterial canker caused by Xanthomonas axonopodis pv. citri is a devastating disease resulting in significant crop losses in various citrus cultivars worldwide. A biocontrol agent has not been recommended for this disease. To explore the potential of bacilli native to Taiwan to control this disease, Bacillus species with a broad spectrum of antagonistic activity against various phytopathogens were isolated from plant potting mixes, organic compost and the rhizosphere soil. Seven strains TKS1-1, OF3-16, SP4-17, HSP1, WG6-14, TLB7-7, and WP8-12 showing superior antagonistic activity were chosen for biopesticide development. The genetic identity based on 16S rDNA sequences indicated that all seven native strains were close relatives of the B. subtilis group and appeared to be discrete from the B. cereus group. DNA polymorphisms in strains WG6-14, SP4-17, TKS1-1, and WP8-12, as revealed by repetitive sequence-based PCR with the BOXA1R primers were similar to each other, but different from those of the respective Bacillus type strains. However, molecular typing of the strains using either tDNA-intergenic spacer regions or 16S-23S intergenic transcribed spacer regions was unable to differentiate the strains at the species level. Strains TKS1-1 and WG6-14 attenuated symptom development of citrus bacterial canker, which was found to be correlated with a reduction in colonization and biofilm formation by X. axonopodis pv. citri on leaf surfaces. The application of a Bacillus strain TKS1-1 endospore formulation to the leaf surfaces of citrus reduced the incidence of citrus bacterial canker and could prevent development of the disease.</abstract><cop>United States</cop><pub>Public Library of Science</pub><pmid>22848728</pmid><doi>10.1371/journal.pone.0042124</doi><tpages>e42124</tpages><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 1932-6203 |
| ispartof | PloS one, 2012-07, Vol.7 (7), p.e42124-e42124 |
| issn | 1932-6203 1932-6203 |
| language | eng |
| recordid | cdi_plos_journals_1325527253 |
| source | MEDLINE; DOAJ Directory of Open Access Journals; Public Library of Science (PLoS) Journals Open Access; EZB-FREE-00999 freely available EZB journals; PubMed Central; Free Full-Text Journals in Chemistry |
| subjects | Agriculture Antibiotics Bacilli Bacillus - classification Bacillus - genetics Bacillus - physiology Bacillus cereus Bacillus subtilis Bacteria Biofilms Biofilms - growth & development Biological control Biological Control Agents Biological pest control Biology Canker Citrus - microbiology Citrus fruits Cloning Cluster Analysis Colonization Composts Cultivars Deoxyribonucleic acid Disease control DNA DNA, Bacterial - genetics DNA, Intergenic - genetics Gene sequencing Genetic aspects Genetic polymorphisms Laboratories Leaves Molecular Sequence Data Pathogens Phyllosphere Phylogeny Plant diseases Plant Diseases - microbiology Plant Leaves - microbiology Plant pathology Polymorphism, Genetic Pseudomonas syringae Repetitive Sequences, Nucleic Acid - genetics Rhizoctonia solani Rhizosphere RNA, Ribosomal, 16S - genetics rRNA 16S Sequence Analysis, RNA Soil Microbiology Strains (organisms) Xanthomonas axonopodis Xanthomonas axonopodis - physiology |
| title | DNA polymorphisms and biocontrol of Bacillus antagonistic to citrus bacterial canker with indication of the interference of phyllosphere biofilms |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2024-12-20T05%3A41%3A56IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-gale_plos_&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=DNA%20polymorphisms%20and%20biocontrol%20of%20Bacillus%20antagonistic%20to%20citrus%20bacterial%20canker%20with%20indication%20of%20the%20interference%20of%20phyllosphere%20biofilms&rft.jtitle=PloS%20one&rft.au=Huang,%20Tzu-Pi&rft.date=2012-07-27&rft.volume=7&rft.issue=7&rft.spage=e42124&rft.epage=e42124&rft.pages=e42124-e42124&rft.issn=1932-6203&rft.eissn=1932-6203&rft_id=info:doi/10.1371/journal.pone.0042124&rft_dat=%3Cgale_plos_%3EA498244922%3C/gale_plos_%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=1325527253&rft_id=info:pmid/22848728&rft_galeid=A498244922&rft_doaj_id=oai_doaj_org_article_bdf6fbc328f9426086da8e0a73bc29d1&rfr_iscdi=true |