Genomic characterization of a novel virus of the family Tymoviridae isolated from mosquitoes
The family Tymoviridae comprises three plant virus genera, including Tymovirus, Marafivirus, and Maculavirus, which are found in most parts of the world and cause severe agricultural losses. We describe a putatively novel member of the family Tymoviridae, which is isolated from mosquitoes (Culex spp...
Gespeichert in:
| Veröffentlicht in: | PloS one 2012-07, Vol.7 (7), p.e39845-e39845 |
|---|---|
| Hauptverfasser: | , , , , , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | e39845 |
|---|---|
| container_issue | 7 |
| container_start_page | e39845 |
| container_title | PloS one |
| container_volume | 7 |
| creator | Wang, Lihua Lv, Xinjun Zhai, Yougang Fu, Shihong Wang, David Rayner, Simon Tang, Qing Liang, Guodong |
| description | The family Tymoviridae comprises three plant virus genera, including Tymovirus, Marafivirus, and Maculavirus, which are found in most parts of the world and cause severe agricultural losses. We describe a putatively novel member of the family Tymoviridae, which is isolated from mosquitoes (Culex spp.), referred to as CuTLV.
The CuTLV was isolated by cell culture, which replicates and causes cytopathic effects in Aedes albopictus C6/36 cells, but not in mammalian BHK-21 or Vero cells. The complete 6471 nucleotide sequence of CuTLV was determined. The genome of CuTLV is predicted to contain three open reading frames (ORFs). The largest ORF1 is 5307 nucleotides (nt) in length and encodes a putative polypeptide of 1769 amino acids (aa), which contains the conserved motifs for the methyltransferase (MTR), Tymovirus endopeptidase (PRO), helicase (HEL), and RNA-dependent RNA polymerase (RdRp) of the replication-associated proteins (RPs) of positive-stranded RNA viruses. In contrast, the ORF1 sequence does not contain the so-called "tymobox" or "marafibox", the conserved subgenomic RNA promoter present in all tymoviruses or marafiviruses, respectively. ORF2 and ORF3 putatively encode a 248-aa coat protein (CP) and a proline-rich 149-aa polypeptide. The whole genomic nucleotide identity of CuTLV with other members of family Tymoviridae ranged from 46.2% (ChiYMV) to 52.4% (GFkV). Phylogenetic analysis based on the putative RP and CP genes of CuTLV demonstrated that the virus is most closely related to viruses in the genus Maculavirus.
The CuTLV is a novel virus related to members of the family Tymoviridae, with molecular characters that are distinct from those of tymoviruses, marafiviruses, and other maculaviruses or macula-like viruses. This is the first report of the isolation of a Tymoviridae-like virus from mosquitoes. Further investigations are required to clarify the origin, replication strategy, and the public health or agricultural importance of the CuTLV. |
| doi_str_mv | 10.1371/journal.pone.0039845 |
| format | Article |
| fullrecord | <record><control><sourceid>gale_plos_</sourceid><recordid>TN_cdi_plos_journals_1325464020</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><galeid>A498244902</galeid><doaj_id>oai_doaj_org_article_a342acde229a494399fd0dee1742859f</doaj_id><sourcerecordid>A498244902</sourcerecordid><originalsourceid>FETCH-LOGICAL-c758t-8cb96a48635a3d8c1335afcf7687f4c67e439a388551feff5b704f16156ba15a3</originalsourceid><addsrcrecordid>eNqNk1GL1DAQx4so3rn6DUQLgujDrkmTpumLcBx6Lhwc6OmTEGbTyW6OttlL0sX105t1e8eu3IPkoWH6-_8nmcxk2UtKZpRV9MONG3wP7WztepwRwmrJy0fZKa1ZMRUFYY8P9ifZsxBuCCmZFOJpdlIUkksm2Gn28wJ711md6xV40BG9_Q3Ruj53Joe8dxts8431Q9gF4gpzA51tt_n1tnMpbhvA3AbXQsQmN951eefC7WCjw_A8e2KgDfhi_E6y758_XZ9_mV5eXczPzy6nuiplnEq9qAVwKVgJrJGasrQx2lRCVoZrUSFnNTApy5IaNKZcVIQbKmgpFkCTZpK93vuuWxfUWJigKCtKLjhJBZhk8z3ROLhRa2878FvlwKq_AeeXCny0ukUFjBegGyyKGnidMtemIQ0irXghy9okr49jtmHRYaOxjx7aI9PjP71dqaXbKMZJVRCRDN6NBt7dDhii6mzQ2LbQoxvSuQkjJeGcyYS--Qd9-HYjtYR0Adsbl_Lqnak647UsOK9JkajZA1RaDaYGSF1kbIofCd4fCRIT8VdcwhCCmn_7-v_s1Y9j9u0Bu0Jo4yp10LBru3AM8j2ovQvBo7kvMiVqNwR31VC7IVDjECTZq8MHuhfddT37A6EJAe4</addsrcrecordid><sourcetype>Open Website</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>1325464020</pqid></control><display><type>article</type><title>Genomic characterization of a novel virus of the family Tymoviridae isolated from mosquitoes</title><source>MEDLINE</source><source>DOAJ Directory of Open Access Journals</source><source>Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals</source><source>PubMed Central</source><source>Free Full-Text Journals in Chemistry</source><source>Public Library of Science (PLoS)</source><creator>Wang, Lihua ; Lv, Xinjun ; Zhai, Yougang ; Fu, Shihong ; Wang, David ; Rayner, Simon ; Tang, Qing ; Liang, Guodong</creator><creatorcontrib>Wang, Lihua ; Lv, Xinjun ; Zhai, Yougang ; Fu, Shihong ; Wang, David ; Rayner, Simon ; Tang, Qing ; Liang, Guodong</creatorcontrib><description>The family Tymoviridae comprises three plant virus genera, including Tymovirus, Marafivirus, and Maculavirus, which are found in most parts of the world and cause severe agricultural losses. We describe a putatively novel member of the family Tymoviridae, which is isolated from mosquitoes (Culex spp.), referred to as CuTLV.
The CuTLV was isolated by cell culture, which replicates and causes cytopathic effects in Aedes albopictus C6/36 cells, but not in mammalian BHK-21 or Vero cells. The complete 6471 nucleotide sequence of CuTLV was determined. The genome of CuTLV is predicted to contain three open reading frames (ORFs). The largest ORF1 is 5307 nucleotides (nt) in length and encodes a putative polypeptide of 1769 amino acids (aa), which contains the conserved motifs for the methyltransferase (MTR), Tymovirus endopeptidase (PRO), helicase (HEL), and RNA-dependent RNA polymerase (RdRp) of the replication-associated proteins (RPs) of positive-stranded RNA viruses. In contrast, the ORF1 sequence does not contain the so-called "tymobox" or "marafibox", the conserved subgenomic RNA promoter present in all tymoviruses or marafiviruses, respectively. ORF2 and ORF3 putatively encode a 248-aa coat protein (CP) and a proline-rich 149-aa polypeptide. The whole genomic nucleotide identity of CuTLV with other members of family Tymoviridae ranged from 46.2% (ChiYMV) to 52.4% (GFkV). Phylogenetic analysis based on the putative RP and CP genes of CuTLV demonstrated that the virus is most closely related to viruses in the genus Maculavirus.
The CuTLV is a novel virus related to members of the family Tymoviridae, with molecular characters that are distinct from those of tymoviruses, marafiviruses, and other maculaviruses or macula-like viruses. This is the first report of the isolation of a Tymoviridae-like virus from mosquitoes. Further investigations are required to clarify the origin, replication strategy, and the public health or agricultural importance of the CuTLV.</description><identifier>ISSN: 1932-6203</identifier><identifier>EISSN: 1932-6203</identifier><identifier>DOI: 10.1371/journal.pone.0039845</identifier><identifier>PMID: 22848363</identifier><language>eng</language><publisher>United States: Public Library of Science</publisher><subject>Amino acids ; Animals ; Aquatic insects ; Biology ; Brassica rapa ; Cell culture ; Cell Line ; Cladistic analysis ; Coat protein ; Conserved sequence ; Cricetinae ; Culex ; Culex - virology ; Culicidae ; Disease control ; Disease prevention ; DNA helicase ; DNA-directed RNA polymerase ; Endopeptidase ; Genes ; Genome, Viral - physiology ; Genomes ; Genomics ; Infectious diseases ; Laboratories ; Methyltransferase ; Mosquitoes ; Nucleotide sequence ; Nucleotides ; Open reading frames ; Open Reading Frames - physiology ; Phylogeny ; Plant viruses ; Proline ; Proteins ; Public health ; Replication ; Replication origins ; Ribonucleic acid ; RNA ; RNA helicase ; RNA viruses ; RNA-directed RNA polymerase ; Tymoviridae - classification ; Tymoviridae - genetics ; Tymoviridae - isolation & purification ; Tymoviridae - metabolism ; Vero cells ; Viral Proteins - genetics ; Viral Proteins - metabolism ; Virology ; Viruses</subject><ispartof>PloS one, 2012-07, Vol.7 (7), p.e39845-e39845</ispartof><rights>COPYRIGHT 2012 Public Library of Science</rights><rights>Wang et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License: https://creativecommons.org/licenses/by/4.0/ (the “License”), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><rights>2012 Wang et al 2012 Wang et al</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c758t-8cb96a48635a3d8c1335afcf7687f4c67e439a388551feff5b704f16156ba15a3</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC3407206/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC3407206/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,723,776,780,860,881,2096,2915,23845,27901,27902,53766,53768,79342,79343</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/22848363$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Wang, Lihua</creatorcontrib><creatorcontrib>Lv, Xinjun</creatorcontrib><creatorcontrib>Zhai, Yougang</creatorcontrib><creatorcontrib>Fu, Shihong</creatorcontrib><creatorcontrib>Wang, David</creatorcontrib><creatorcontrib>Rayner, Simon</creatorcontrib><creatorcontrib>Tang, Qing</creatorcontrib><creatorcontrib>Liang, Guodong</creatorcontrib><title>Genomic characterization of a novel virus of the family Tymoviridae isolated from mosquitoes</title><title>PloS one</title><addtitle>PLoS One</addtitle><description>The family Tymoviridae comprises three plant virus genera, including Tymovirus, Marafivirus, and Maculavirus, which are found in most parts of the world and cause severe agricultural losses. We describe a putatively novel member of the family Tymoviridae, which is isolated from mosquitoes (Culex spp.), referred to as CuTLV.
The CuTLV was isolated by cell culture, which replicates and causes cytopathic effects in Aedes albopictus C6/36 cells, but not in mammalian BHK-21 or Vero cells. The complete 6471 nucleotide sequence of CuTLV was determined. The genome of CuTLV is predicted to contain three open reading frames (ORFs). The largest ORF1 is 5307 nucleotides (nt) in length and encodes a putative polypeptide of 1769 amino acids (aa), which contains the conserved motifs for the methyltransferase (MTR), Tymovirus endopeptidase (PRO), helicase (HEL), and RNA-dependent RNA polymerase (RdRp) of the replication-associated proteins (RPs) of positive-stranded RNA viruses. In contrast, the ORF1 sequence does not contain the so-called "tymobox" or "marafibox", the conserved subgenomic RNA promoter present in all tymoviruses or marafiviruses, respectively. ORF2 and ORF3 putatively encode a 248-aa coat protein (CP) and a proline-rich 149-aa polypeptide. The whole genomic nucleotide identity of CuTLV with other members of family Tymoviridae ranged from 46.2% (ChiYMV) to 52.4% (GFkV). Phylogenetic analysis based on the putative RP and CP genes of CuTLV demonstrated that the virus is most closely related to viruses in the genus Maculavirus.
The CuTLV is a novel virus related to members of the family Tymoviridae, with molecular characters that are distinct from those of tymoviruses, marafiviruses, and other maculaviruses or macula-like viruses. This is the first report of the isolation of a Tymoviridae-like virus from mosquitoes. Further investigations are required to clarify the origin, replication strategy, and the public health or agricultural importance of the CuTLV.</description><subject>Amino acids</subject><subject>Animals</subject><subject>Aquatic insects</subject><subject>Biology</subject><subject>Brassica rapa</subject><subject>Cell culture</subject><subject>Cell Line</subject><subject>Cladistic analysis</subject><subject>Coat protein</subject><subject>Conserved sequence</subject><subject>Cricetinae</subject><subject>Culex</subject><subject>Culex - virology</subject><subject>Culicidae</subject><subject>Disease control</subject><subject>Disease prevention</subject><subject>DNA helicase</subject><subject>DNA-directed RNA polymerase</subject><subject>Endopeptidase</subject><subject>Genes</subject><subject>Genome, Viral - physiology</subject><subject>Genomes</subject><subject>Genomics</subject><subject>Infectious diseases</subject><subject>Laboratories</subject><subject>Methyltransferase</subject><subject>Mosquitoes</subject><subject>Nucleotide sequence</subject><subject>Nucleotides</subject><subject>Open reading frames</subject><subject>Open Reading Frames - physiology</subject><subject>Phylogeny</subject><subject>Plant viruses</subject><subject>Proline</subject><subject>Proteins</subject><subject>Public health</subject><subject>Replication</subject><subject>Replication origins</subject><subject>Ribonucleic acid</subject><subject>RNA</subject><subject>RNA helicase</subject><subject>RNA viruses</subject><subject>RNA-directed RNA polymerase</subject><subject>Tymoviridae - classification</subject><subject>Tymoviridae - genetics</subject><subject>Tymoviridae - isolation & purification</subject><subject>Tymoviridae - metabolism</subject><subject>Vero cells</subject><subject>Viral Proteins - genetics</subject><subject>Viral Proteins - metabolism</subject><subject>Virology</subject><subject>Viruses</subject><issn>1932-6203</issn><issn>1932-6203</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2012</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>BENPR</sourceid><sourceid>DOA</sourceid><recordid>eNqNk1GL1DAQx4so3rn6DUQLgujDrkmTpumLcBx6Lhwc6OmTEGbTyW6OttlL0sX105t1e8eu3IPkoWH6-_8nmcxk2UtKZpRV9MONG3wP7WztepwRwmrJy0fZKa1ZMRUFYY8P9ifZsxBuCCmZFOJpdlIUkksm2Gn28wJ711md6xV40BG9_Q3Ruj53Joe8dxts8431Q9gF4gpzA51tt_n1tnMpbhvA3AbXQsQmN951eefC7WCjw_A8e2KgDfhi_E6y758_XZ9_mV5eXczPzy6nuiplnEq9qAVwKVgJrJGasrQx2lRCVoZrUSFnNTApy5IaNKZcVIQbKmgpFkCTZpK93vuuWxfUWJigKCtKLjhJBZhk8z3ROLhRa2878FvlwKq_AeeXCny0ukUFjBegGyyKGnidMtemIQ0irXghy9okr49jtmHRYaOxjx7aI9PjP71dqaXbKMZJVRCRDN6NBt7dDhii6mzQ2LbQoxvSuQkjJeGcyYS--Qd9-HYjtYR0Adsbl_Lqnak647UsOK9JkajZA1RaDaYGSF1kbIofCd4fCRIT8VdcwhCCmn_7-v_s1Y9j9u0Bu0Jo4yp10LBru3AM8j2ovQvBo7kvMiVqNwR31VC7IVDjECTZq8MHuhfddT37A6EJAe4</recordid><startdate>20120727</startdate><enddate>20120727</enddate><creator>Wang, Lihua</creator><creator>Lv, Xinjun</creator><creator>Zhai, Yougang</creator><creator>Fu, Shihong</creator><creator>Wang, David</creator><creator>Rayner, Simon</creator><creator>Tang, Qing</creator><creator>Liang, Guodong</creator><general>Public Library of Science</general><general>Public Library of Science (PLoS)</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>IOV</scope><scope>ISR</scope><scope>3V.</scope><scope>7QG</scope><scope>7QL</scope><scope>7QO</scope><scope>7RV</scope><scope>7SN</scope><scope>7SS</scope><scope>7T5</scope><scope>7TG</scope><scope>7TM</scope><scope>7U9</scope><scope>7X2</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>8AO</scope><scope>8C1</scope><scope>8FD</scope><scope>8FE</scope><scope>8FG</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABJCF</scope><scope>ABUWG</scope><scope>AEUYN</scope><scope>AFKRA</scope><scope>ARAPS</scope><scope>ATCPS</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BGLVJ</scope><scope>BHPHI</scope><scope>C1K</scope><scope>CCPQU</scope><scope>D1I</scope><scope>DWQXO</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>H94</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>KB.</scope><scope>KB0</scope><scope>KL.</scope><scope>L6V</scope><scope>LK8</scope><scope>M0K</scope><scope>M0S</scope><scope>M1P</scope><scope>M7N</scope><scope>M7P</scope><scope>M7S</scope><scope>NAPCQ</scope><scope>P5Z</scope><scope>P62</scope><scope>P64</scope><scope>PATMY</scope><scope>PDBOC</scope><scope>PIMPY</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PTHSS</scope><scope>PYCSY</scope><scope>RC3</scope><scope>7X8</scope><scope>5PM</scope><scope>DOA</scope></search><sort><creationdate>20120727</creationdate><title>Genomic characterization of a novel virus of the family Tymoviridae isolated from mosquitoes</title><author>Wang, Lihua ; Lv, Xinjun ; Zhai, Yougang ; Fu, Shihong ; Wang, David ; Rayner, Simon ; Tang, Qing ; Liang, Guodong</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c758t-8cb96a48635a3d8c1335afcf7687f4c67e439a388551feff5b704f16156ba15a3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2012</creationdate><topic>Amino acids</topic><topic>Animals</topic><topic>Aquatic insects</topic><topic>Biology</topic><topic>Brassica rapa</topic><topic>Cell culture</topic><topic>Cell Line</topic><topic>Cladistic analysis</topic><topic>Coat protein</topic><topic>Conserved sequence</topic><topic>Cricetinae</topic><topic>Culex</topic><topic>Culex - virology</topic><topic>Culicidae</topic><topic>Disease control</topic><topic>Disease prevention</topic><topic>DNA helicase</topic><topic>DNA-directed RNA polymerase</topic><topic>Endopeptidase</topic><topic>Genes</topic><topic>Genome, Viral - physiology</topic><topic>Genomes</topic><topic>Genomics</topic><topic>Infectious diseases</topic><topic>Laboratories</topic><topic>Methyltransferase</topic><topic>Mosquitoes</topic><topic>Nucleotide sequence</topic><topic>Nucleotides</topic><topic>Open reading frames</topic><topic>Open Reading Frames - physiology</topic><topic>Phylogeny</topic><topic>Plant viruses</topic><topic>Proline</topic><topic>Proteins</topic><topic>Public health</topic><topic>Replication</topic><topic>Replication origins</topic><topic>Ribonucleic acid</topic><topic>RNA</topic><topic>RNA helicase</topic><topic>RNA viruses</topic><topic>RNA-directed RNA polymerase</topic><topic>Tymoviridae - classification</topic><topic>Tymoviridae - genetics</topic><topic>Tymoviridae - isolation & purification</topic><topic>Tymoviridae - metabolism</topic><topic>Vero cells</topic><topic>Viral Proteins - genetics</topic><topic>Viral Proteins - metabolism</topic><topic>Virology</topic><topic>Viruses</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Wang, Lihua</creatorcontrib><creatorcontrib>Lv, Xinjun</creatorcontrib><creatorcontrib>Zhai, Yougang</creatorcontrib><creatorcontrib>Fu, Shihong</creatorcontrib><creatorcontrib>Wang, David</creatorcontrib><creatorcontrib>Rayner, Simon</creatorcontrib><creatorcontrib>Tang, Qing</creatorcontrib><creatorcontrib>Liang, Guodong</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Gale In Context: Opposing Viewpoints</collection><collection>Gale In Context: Science</collection><collection>ProQuest Central (Corporate)</collection><collection>Animal Behavior Abstracts</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Biotechnology Research Abstracts</collection><collection>Nursing & Allied Health Database</collection><collection>Ecology Abstracts</collection><collection>Entomology Abstracts (Full archive)</collection><collection>Immunology Abstracts</collection><collection>Meteorological & Geoastrophysical Abstracts</collection><collection>Nucleic Acids Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>Agricultural Science Collection</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Medical Database (Alumni Edition)</collection><collection>ProQuest Pharma Collection</collection><collection>Public Health Database</collection><collection>Technology Research Database</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Technology Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>Materials Science & Engineering Collection</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest One Sustainability</collection><collection>ProQuest Central UK/Ireland</collection><collection>Advanced Technologies & Aerospace Collection</collection><collection>Agricultural & Environmental Science Collection</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Technology Collection</collection><collection>Natural Science Collection</collection><collection>Environmental Sciences and Pollution Management</collection><collection>ProQuest One Community College</collection><collection>ProQuest Materials Science Collection</collection><collection>ProQuest Central Korea</collection><collection>Engineering Research Database</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Materials Science Database</collection><collection>Nursing & Allied Health Database (Alumni Edition)</collection><collection>Meteorological & Geoastrophysical Abstracts - Academic</collection><collection>ProQuest Engineering Collection</collection><collection>ProQuest Biological Science Collection</collection><collection>Agricultural Science Database</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biological Science Database</collection><collection>Engineering Database</collection><collection>Nursing & Allied Health Premium</collection><collection>Advanced Technologies & Aerospace Database</collection><collection>ProQuest Advanced Technologies & Aerospace Collection</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Environmental Science Database</collection><collection>Materials Science Collection</collection><collection>Publicly Available Content Database</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>Engineering Collection</collection><collection>Environmental Science Collection</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><collection>DOAJ Directory of Open Access Journals</collection><jtitle>PloS one</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Wang, Lihua</au><au>Lv, Xinjun</au><au>Zhai, Yougang</au><au>Fu, Shihong</au><au>Wang, David</au><au>Rayner, Simon</au><au>Tang, Qing</au><au>Liang, Guodong</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Genomic characterization of a novel virus of the family Tymoviridae isolated from mosquitoes</atitle><jtitle>PloS one</jtitle><addtitle>PLoS One</addtitle><date>2012-07-27</date><risdate>2012</risdate><volume>7</volume><issue>7</issue><spage>e39845</spage><epage>e39845</epage><pages>e39845-e39845</pages><issn>1932-6203</issn><eissn>1932-6203</eissn><abstract>The family Tymoviridae comprises three plant virus genera, including Tymovirus, Marafivirus, and Maculavirus, which are found in most parts of the world and cause severe agricultural losses. We describe a putatively novel member of the family Tymoviridae, which is isolated from mosquitoes (Culex spp.), referred to as CuTLV.
The CuTLV was isolated by cell culture, which replicates and causes cytopathic effects in Aedes albopictus C6/36 cells, but not in mammalian BHK-21 or Vero cells. The complete 6471 nucleotide sequence of CuTLV was determined. The genome of CuTLV is predicted to contain three open reading frames (ORFs). The largest ORF1 is 5307 nucleotides (nt) in length and encodes a putative polypeptide of 1769 amino acids (aa), which contains the conserved motifs for the methyltransferase (MTR), Tymovirus endopeptidase (PRO), helicase (HEL), and RNA-dependent RNA polymerase (RdRp) of the replication-associated proteins (RPs) of positive-stranded RNA viruses. In contrast, the ORF1 sequence does not contain the so-called "tymobox" or "marafibox", the conserved subgenomic RNA promoter present in all tymoviruses or marafiviruses, respectively. ORF2 and ORF3 putatively encode a 248-aa coat protein (CP) and a proline-rich 149-aa polypeptide. The whole genomic nucleotide identity of CuTLV with other members of family Tymoviridae ranged from 46.2% (ChiYMV) to 52.4% (GFkV). Phylogenetic analysis based on the putative RP and CP genes of CuTLV demonstrated that the virus is most closely related to viruses in the genus Maculavirus.
The CuTLV is a novel virus related to members of the family Tymoviridae, with molecular characters that are distinct from those of tymoviruses, marafiviruses, and other maculaviruses or macula-like viruses. This is the first report of the isolation of a Tymoviridae-like virus from mosquitoes. Further investigations are required to clarify the origin, replication strategy, and the public health or agricultural importance of the CuTLV.</abstract><cop>United States</cop><pub>Public Library of Science</pub><pmid>22848363</pmid><doi>10.1371/journal.pone.0039845</doi><tpages>e39845</tpages><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 1932-6203 |
| ispartof | PloS one, 2012-07, Vol.7 (7), p.e39845-e39845 |
| issn | 1932-6203 1932-6203 |
| language | eng |
| recordid | cdi_plos_journals_1325464020 |
| source | MEDLINE; DOAJ Directory of Open Access Journals; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; PubMed Central; Free Full-Text Journals in Chemistry; Public Library of Science (PLoS) |
| subjects | Amino acids Animals Aquatic insects Biology Brassica rapa Cell culture Cell Line Cladistic analysis Coat protein Conserved sequence Cricetinae Culex Culex - virology Culicidae Disease control Disease prevention DNA helicase DNA-directed RNA polymerase Endopeptidase Genes Genome, Viral - physiology Genomes Genomics Infectious diseases Laboratories Methyltransferase Mosquitoes Nucleotide sequence Nucleotides Open reading frames Open Reading Frames - physiology Phylogeny Plant viruses Proline Proteins Public health Replication Replication origins Ribonucleic acid RNA RNA helicase RNA viruses RNA-directed RNA polymerase Tymoviridae - classification Tymoviridae - genetics Tymoviridae - isolation & purification Tymoviridae - metabolism Vero cells Viral Proteins - genetics Viral Proteins - metabolism Virology Viruses |
| title | Genomic characterization of a novel virus of the family Tymoviridae isolated from mosquitoes |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-02-05T09%3A04%3A05IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-gale_plos_&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Genomic%20characterization%20of%20a%20novel%20virus%20of%20the%20family%20Tymoviridae%20isolated%20from%20mosquitoes&rft.jtitle=PloS%20one&rft.au=Wang,%20Lihua&rft.date=2012-07-27&rft.volume=7&rft.issue=7&rft.spage=e39845&rft.epage=e39845&rft.pages=e39845-e39845&rft.issn=1932-6203&rft.eissn=1932-6203&rft_id=info:doi/10.1371/journal.pone.0039845&rft_dat=%3Cgale_plos_%3EA498244902%3C/gale_plos_%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=1325464020&rft_id=info:pmid/22848363&rft_galeid=A498244902&rft_doaj_id=oai_doaj_org_article_a342acde229a494399fd0dee1742859f&rfr_iscdi=true |