Screening of 71 P. multocida proteins for protective efficacy in a fowl cholera infection model and characterization of the protective antigen PlpE
There is a strong need for a recombinant subunit vaccine against fowl cholera. We used a reverse vaccinology approach to identify putative secreted or cell surface associated P. multocida proteins that may represent potential vaccine candidate antigens. A high-throughput cloning and expression proto...
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| Veröffentlicht in: | PloS one 2012-07, Vol.7 (7), p.e39973-e39973 |
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| creator | Hatfaludi, Tamás Al-Hasani, Keith Gong, Lan Boyce, John D Ford, Mark Wilkie, Ian W Quinsey, Noelene Dunstone, Michelle A Hoke, David E Adler, Ben |
| description | There is a strong need for a recombinant subunit vaccine against fowl cholera. We used a reverse vaccinology approach to identify putative secreted or cell surface associated P. multocida proteins that may represent potential vaccine candidate antigens.
A high-throughput cloning and expression protocol was used to express and purify 71 recombinant proteins for vaccine trials. Of the 71 proteins tested, only one, PlpE in denatured insoluble form, protected chickens against fowl cholera challenge. PlpE also elicited comparable levels of protection in mice. PlpE was localized by immunofluorescence to the bacterial cell surface, consistent with its ability to elicit a protective immune response. To explore the role of PlpE during infection and immunity, a plpE mutant was generated. The plpE mutant strain retained full virulence for mice.
These studies show that PlpE is a surface exposed protein and was the only protein of 71 tested that was able to elicit a protective immune response. However, PlpE is not an essential virulence factor. This is the first report of a denatured recombinant protein stimulating protection against fowl cholera. |
| doi_str_mv | 10.1371/journal.pone.0039973 |
| format | Article |
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A high-throughput cloning and expression protocol was used to express and purify 71 recombinant proteins for vaccine trials. Of the 71 proteins tested, only one, PlpE in denatured insoluble form, protected chickens against fowl cholera challenge. PlpE also elicited comparable levels of protection in mice. PlpE was localized by immunofluorescence to the bacterial cell surface, consistent with its ability to elicit a protective immune response. To explore the role of PlpE during infection and immunity, a plpE mutant was generated. The plpE mutant strain retained full virulence for mice.
These studies show that PlpE is a surface exposed protein and was the only protein of 71 tested that was able to elicit a protective immune response. However, PlpE is not an essential virulence factor. This is the first report of a denatured recombinant protein stimulating protection against fowl cholera.</description><identifier>ISSN: 1932-6203</identifier><identifier>EISSN: 1932-6203</identifier><identifier>DOI: 10.1371/journal.pone.0039973</identifier><identifier>PMID: 22792202</identifier><language>eng</language><publisher>United States: Public Library of Science</publisher><subject><![CDATA[Agriculture ; Animals ; Antigens ; Antigens, Bacterial - genetics ; Antigens, Bacterial - immunology ; Antigens, Bacterial - isolation & purification ; Bacterial Outer Membrane Proteins - genetics ; Bacterial Outer Membrane Proteins - immunology ; Bacterial Outer Membrane Proteins - isolation & purification ; Bacterial Vaccines - immunology ; Biochemistry ; Bioinformatics ; Biology ; Cell surface ; Chickens ; Chickens - immunology ; Chickens - microbiology ; Cholera ; Cloning ; Councils ; Disease Models, Animal ; E coli ; Ehrlichia chaffeensis ; Female ; Fowl cholera ; Gene Expression ; Genomes ; Genomics ; Identification ; Immune response ; Immune system ; Immunity ; Immunofluorescence ; Infections ; Laboratories ; Medical screening ; Medicine ; Mice ; Molecular biology ; Mutant Proteins - genetics ; Mutant Proteins - immunology ; Mutant Proteins - isolation & purification ; Mutation ; Pasteurella ; Pasteurella Infections - prevention & control ; Pasteurella Infections - veterinary ; Pasteurella multocida ; Pasteurella multocida - genetics ; Pasteurella multocida - immunology ; Pasteurella multocida - pathogenicity ; Plasmids ; Poultry ; Poultry Diseases - prevention & control ; Protective antigen ; Proteins ; Public health ; Recombinant proteins ; Recombinant Proteins - genetics ; Recombinant Proteins - immunology ; Recombinant Proteins - isolation & purification ; Sepsis ; Vaccines ; Vaccines, Synthetic - immunology ; Veterinary Science ; Vibrio cholerae ; Virulence ; Virulence (Microbiology) ; Virulence factors ; Virulence Factors - genetics ; Virulence Factors - immunology ; Virulence Factors - isolation & purification ; Waterborne diseases]]></subject><ispartof>PloS one, 2012-07, Vol.7 (7), p.e39973-e39973</ispartof><rights>COPYRIGHT 2012 Public Library of Science</rights><rights>2012 Hatfaludi et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License: https://creativecommons.org/licenses/by/4.0/ (the “License”), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><rights>Hatfaludi et al. 2012</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c692t-4f24b2cf01f941505df98cd9166897bbdf07720a9ceee8f1088d35722f9967a23</citedby><cites>FETCH-LOGICAL-c692t-4f24b2cf01f941505df98cd9166897bbdf07720a9ceee8f1088d35722f9967a23</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC3390355/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC3390355/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,723,776,780,860,881,2096,2915,23845,27901,27902,53766,53768,79343,79344</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/22792202$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Hatfaludi, Tamás</creatorcontrib><creatorcontrib>Al-Hasani, Keith</creatorcontrib><creatorcontrib>Gong, Lan</creatorcontrib><creatorcontrib>Boyce, John D</creatorcontrib><creatorcontrib>Ford, Mark</creatorcontrib><creatorcontrib>Wilkie, Ian W</creatorcontrib><creatorcontrib>Quinsey, Noelene</creatorcontrib><creatorcontrib>Dunstone, Michelle A</creatorcontrib><creatorcontrib>Hoke, David E</creatorcontrib><creatorcontrib>Adler, Ben</creatorcontrib><title>Screening of 71 P. multocida proteins for protective efficacy in a fowl cholera infection model and characterization of the protective antigen PlpE</title><title>PloS one</title><addtitle>PLoS One</addtitle><description>There is a strong need for a recombinant subunit vaccine against fowl cholera. We used a reverse vaccinology approach to identify putative secreted or cell surface associated P. multocida proteins that may represent potential vaccine candidate antigens.
A high-throughput cloning and expression protocol was used to express and purify 71 recombinant proteins for vaccine trials. Of the 71 proteins tested, only one, PlpE in denatured insoluble form, protected chickens against fowl cholera challenge. PlpE also elicited comparable levels of protection in mice. PlpE was localized by immunofluorescence to the bacterial cell surface, consistent with its ability to elicit a protective immune response. To explore the role of PlpE during infection and immunity, a plpE mutant was generated. The plpE mutant strain retained full virulence for mice.
These studies show that PlpE is a surface exposed protein and was the only protein of 71 tested that was able to elicit a protective immune response. However, PlpE is not an essential virulence factor. This is the first report of a denatured recombinant protein stimulating protection against fowl cholera.</description><subject>Agriculture</subject><subject>Animals</subject><subject>Antigens</subject><subject>Antigens, Bacterial - genetics</subject><subject>Antigens, Bacterial - immunology</subject><subject>Antigens, Bacterial - isolation & purification</subject><subject>Bacterial Outer Membrane Proteins - genetics</subject><subject>Bacterial Outer Membrane Proteins - immunology</subject><subject>Bacterial Outer Membrane Proteins - isolation & purification</subject><subject>Bacterial Vaccines - immunology</subject><subject>Biochemistry</subject><subject>Bioinformatics</subject><subject>Biology</subject><subject>Cell surface</subject><subject>Chickens</subject><subject>Chickens - immunology</subject><subject>Chickens - microbiology</subject><subject>Cholera</subject><subject>Cloning</subject><subject>Councils</subject><subject>Disease Models, Animal</subject><subject>E coli</subject><subject>Ehrlichia chaffeensis</subject><subject>Female</subject><subject>Fowl cholera</subject><subject>Gene Expression</subject><subject>Genomes</subject><subject>Genomics</subject><subject>Identification</subject><subject>Immune response</subject><subject>Immune system</subject><subject>Immunity</subject><subject>Immunofluorescence</subject><subject>Infections</subject><subject>Laboratories</subject><subject>Medical screening</subject><subject>Medicine</subject><subject>Mice</subject><subject>Molecular biology</subject><subject>Mutant Proteins - genetics</subject><subject>Mutant Proteins - immunology</subject><subject>Mutant Proteins - isolation & purification</subject><subject>Mutation</subject><subject>Pasteurella</subject><subject>Pasteurella Infections - prevention & control</subject><subject>Pasteurella Infections - veterinary</subject><subject>Pasteurella multocida</subject><subject>Pasteurella multocida - genetics</subject><subject>Pasteurella multocida - immunology</subject><subject>Pasteurella multocida - pathogenicity</subject><subject>Plasmids</subject><subject>Poultry</subject><subject>Poultry Diseases - prevention & control</subject><subject>Protective antigen</subject><subject>Proteins</subject><subject>Public health</subject><subject>Recombinant proteins</subject><subject>Recombinant Proteins - genetics</subject><subject>Recombinant Proteins - immunology</subject><subject>Recombinant Proteins - isolation & purification</subject><subject>Sepsis</subject><subject>Vaccines</subject><subject>Vaccines, Synthetic - immunology</subject><subject>Veterinary Science</subject><subject>Vibrio cholerae</subject><subject>Virulence</subject><subject>Virulence (Microbiology)</subject><subject>Virulence factors</subject><subject>Virulence Factors - 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Academic</collection><collection>PubMed Central (Full Participant titles)</collection><collection>DOAJ Directory of Open Access Journals</collection><jtitle>PloS one</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Hatfaludi, Tamás</au><au>Al-Hasani, Keith</au><au>Gong, Lan</au><au>Boyce, John D</au><au>Ford, Mark</au><au>Wilkie, Ian W</au><au>Quinsey, Noelene</au><au>Dunstone, Michelle A</au><au>Hoke, David E</au><au>Adler, Ben</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Screening of 71 P. multocida proteins for protective efficacy in a fowl cholera infection model and characterization of the protective antigen PlpE</atitle><jtitle>PloS one</jtitle><addtitle>PLoS One</addtitle><date>2012-07-05</date><risdate>2012</risdate><volume>7</volume><issue>7</issue><spage>e39973</spage><epage>e39973</epage><pages>e39973-e39973</pages><issn>1932-6203</issn><eissn>1932-6203</eissn><abstract>There is a strong need for a recombinant subunit vaccine against fowl cholera. We used a reverse vaccinology approach to identify putative secreted or cell surface associated P. multocida proteins that may represent potential vaccine candidate antigens.
A high-throughput cloning and expression protocol was used to express and purify 71 recombinant proteins for vaccine trials. Of the 71 proteins tested, only one, PlpE in denatured insoluble form, protected chickens against fowl cholera challenge. PlpE also elicited comparable levels of protection in mice. PlpE was localized by immunofluorescence to the bacterial cell surface, consistent with its ability to elicit a protective immune response. To explore the role of PlpE during infection and immunity, a plpE mutant was generated. The plpE mutant strain retained full virulence for mice.
These studies show that PlpE is a surface exposed protein and was the only protein of 71 tested that was able to elicit a protective immune response. However, PlpE is not an essential virulence factor. This is the first report of a denatured recombinant protein stimulating protection against fowl cholera.</abstract><cop>United States</cop><pub>Public Library of Science</pub><pmid>22792202</pmid><doi>10.1371/journal.pone.0039973</doi><tpages>e39973</tpages><oa>free_for_read</oa></addata></record> |
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| recordid | cdi_plos_journals_1325395387 |
| source | MEDLINE; DOAJ Directory of Open Access Journals; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; PubMed Central; Free Full-Text Journals in Chemistry; Public Library of Science (PLoS) |
| subjects | Agriculture Animals Antigens Antigens, Bacterial - genetics Antigens, Bacterial - immunology Antigens, Bacterial - isolation & purification Bacterial Outer Membrane Proteins - genetics Bacterial Outer Membrane Proteins - immunology Bacterial Outer Membrane Proteins - isolation & purification Bacterial Vaccines - immunology Biochemistry Bioinformatics Biology Cell surface Chickens Chickens - immunology Chickens - microbiology Cholera Cloning Councils Disease Models, Animal E coli Ehrlichia chaffeensis Female Fowl cholera Gene Expression Genomes Genomics Identification Immune response Immune system Immunity Immunofluorescence Infections Laboratories Medical screening Medicine Mice Molecular biology Mutant Proteins - genetics Mutant Proteins - immunology Mutant Proteins - isolation & purification Mutation Pasteurella Pasteurella Infections - prevention & control Pasteurella Infections - veterinary Pasteurella multocida Pasteurella multocida - genetics Pasteurella multocida - immunology Pasteurella multocida - pathogenicity Plasmids Poultry Poultry Diseases - prevention & control Protective antigen Proteins Public health Recombinant proteins Recombinant Proteins - genetics Recombinant Proteins - immunology Recombinant Proteins - isolation & purification Sepsis Vaccines Vaccines, Synthetic - immunology Veterinary Science Vibrio cholerae Virulence Virulence (Microbiology) Virulence factors Virulence Factors - genetics Virulence Factors - immunology Virulence Factors - isolation & purification Waterborne diseases |
| title | Screening of 71 P. multocida proteins for protective efficacy in a fowl cholera infection model and characterization of the protective antigen PlpE |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-02-03T14%3A23%3A25IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-gale_plos_&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Screening%20of%2071%20P.%20multocida%20proteins%20for%20protective%20efficacy%20in%20a%20fowl%20cholera%20infection%20model%20and%20characterization%20of%20the%20protective%20antigen%20PlpE&rft.jtitle=PloS%20one&rft.au=Hatfaludi,%20Tam%C3%A1s&rft.date=2012-07-05&rft.volume=7&rft.issue=7&rft.spage=e39973&rft.epage=e39973&rft.pages=e39973-e39973&rft.issn=1932-6203&rft.eissn=1932-6203&rft_id=info:doi/10.1371/journal.pone.0039973&rft_dat=%3Cgale_plos_%3EA477114797%3C/gale_plos_%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=1325395387&rft_id=info:pmid/22792202&rft_galeid=A477114797&rft_doaj_id=oai_doaj_org_article_484699b934ed4af39dccbfe03cc58423&rfr_iscdi=true |