Rapid and high-throughput detection of highly pathogenic bacteria by Ibis PLEX-ID technology
In this manuscript, we describe the identification of highly pathogenic bacteria using an assay coupling biothreat group-specific PCR with electrospray ionization mass spectrometry (PCR/ESI-MS) run on an Ibis PLEX-ID high-throughput platform. The biothreat cluster assay identifies most of the potent...
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| Veröffentlicht in: | PloS one 2012-06, Vol.7 (6), p.e39928-e39928 |
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| creator | Jacob, Daniela Sauer, Uschi Housley, Roberta Washington, Cicely Sannes-Lowery, Kristin Ecker, David J Sampath, Rangarajan Grunow, Roland |
| description | In this manuscript, we describe the identification of highly pathogenic bacteria using an assay coupling biothreat group-specific PCR with electrospray ionization mass spectrometry (PCR/ESI-MS) run on an Ibis PLEX-ID high-throughput platform. The biothreat cluster assay identifies most of the potential bioterrorism-relevant microorganisms including Bacillus anthracis, Francisella tularensis, Yersinia pestis, Burkholderia mallei and pseudomallei, Brucella species, and Coxiella burnetii. DNA from 45 different reference materials with different formulations and different concentrations were chosen and sent to a service screening laboratory that uses the PCR/ESI-MS platform to provide a microbial identification service. The standard reference materials were produced out of a repository built up in the framework of the EU funded project "Establishment of Quality Assurances for Detection of Highly Pathogenic Bacteria of Potential Bioterrorism Risk" (EQADeBa). All samples were correctly identified at least to the genus level. |
| doi_str_mv | 10.1371/journal.pone.0039928 |
| format | Article |
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All samples were correctly identified at least to the genus level.</description><subject>Automation</subject><subject>Bacillus anthracis</subject><subject>Bacteria</subject><subject>Bacteria - genetics</subject><subject>Bacteria - isolation & purification</subject><subject>Biological & chemical terrorism</subject><subject>Biology</subject><subject>Biosensors</subject><subject>Bioterrorism</subject><subject>Brucella</subject><subject>Burkholderia</subject><subject>Construction standards</subject><subject>Coupling (molecular)</subject><subject>Deoxyribonucleic acid</subject><subject>Disease control</subject><subject>DNA</subject><subject>DNA, Bacterial - genetics</subject><subject>Enzymes</subject><subject>Formulations</subject><subject>Francisella tularensis</subject><subject>Genetic testing</subject><subject>Genome, Bacterial - genetics</subject><subject>Health surveillance</subject><subject>High-Throughput Nucleotide Sequencing - methods</subject><subject>Identification</subject><subject>Infectious diseases</subject><subject>Influenza</subject><subject>Internal controls</subject><subject>Ionization</subject><subject>Ions</subject><subject>Mass spectrometry</subject><subject>Mass spectroscopy</subject><subject>Medicine</subject><subject>Microbial Viability - 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Academic</collection><collection>PubMed Central (Full Participant titles)</collection><collection>DOAJ Directory of Open Access Journals</collection><jtitle>PloS one</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Jacob, Daniela</au><au>Sauer, Uschi</au><au>Housley, Roberta</au><au>Washington, Cicely</au><au>Sannes-Lowery, Kristin</au><au>Ecker, David J</au><au>Sampath, Rangarajan</au><au>Grunow, Roland</au><au>Lin, Baochuan</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Rapid and high-throughput detection of highly pathogenic bacteria by Ibis PLEX-ID technology</atitle><jtitle>PloS one</jtitle><addtitle>PLoS One</addtitle><date>2012-06-29</date><risdate>2012</risdate><volume>7</volume><issue>6</issue><spage>e39928</spage><epage>e39928</epage><pages>e39928-e39928</pages><issn>1932-6203</issn><eissn>1932-6203</eissn><abstract>In this manuscript, we describe the identification of highly pathogenic bacteria using an assay coupling biothreat group-specific PCR with electrospray ionization mass spectrometry (PCR/ESI-MS) run on an Ibis PLEX-ID high-throughput platform. The biothreat cluster assay identifies most of the potential bioterrorism-relevant microorganisms including Bacillus anthracis, Francisella tularensis, Yersinia pestis, Burkholderia mallei and pseudomallei, Brucella species, and Coxiella burnetii. DNA from 45 different reference materials with different formulations and different concentrations were chosen and sent to a service screening laboratory that uses the PCR/ESI-MS platform to provide a microbial identification service. The standard reference materials were produced out of a repository built up in the framework of the EU funded project "Establishment of Quality Assurances for Detection of Highly Pathogenic Bacteria of Potential Bioterrorism Risk" (EQADeBa). All samples were correctly identified at least to the genus level.</abstract><cop>United States</cop><pub>Public Library of Science</pub><pmid>22768173</pmid><doi>10.1371/journal.pone.0039928</doi><tpages>e39928</tpages><oa>free_for_read</oa></addata></record> |
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| subjects | Automation Bacillus anthracis Bacteria Bacteria - genetics Bacteria - isolation & purification Biological & chemical terrorism Biology Biosensors Bioterrorism Brucella Burkholderia Construction standards Coupling (molecular) Deoxyribonucleic acid Disease control DNA DNA, Bacterial - genetics Enzymes Formulations Francisella tularensis Genetic testing Genome, Bacterial - genetics Health surveillance High-Throughput Nucleotide Sequencing - methods Identification Infectious diseases Influenza Internal controls Ionization Ions Mass spectrometry Mass spectroscopy Medicine Microbial Viability - genetics Microorganisms Pathogens Polymerase chain reaction Polymerase Chain Reaction - methods Public health Quality assurance Reference materials Scientific imaging Spectrometry, Mass, Electrospray Ionization - methods Standard reference materials Viruses Websites Yersinia Yersinia pestis Zoonoses |
| title | Rapid and high-throughput detection of highly pathogenic bacteria by Ibis PLEX-ID technology |
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