Ecto-5'-nucleotidase: a candidate virulence factor in Streptococcus sanguinis experimental endocarditis

Streptococcus sanguinis is the most common cause of infective endocarditis (IE). Since the molecular basis of virulence of this oral commensal bacterium remains unclear, we searched the genome of S. sanguinis for previously unidentified virulence factors. We identified a cell surface ecto-5'-nu...

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Veröffentlicht in:	PloS one 2012-06, Vol.7 (6), p.e38059-e38059
Hauptverfasser:	Fan, Jingyuan, Zhang, Yongshu, Chuang-Smith, Olivia N, Frank, Kristi L, Guenther, Brian D, Kern, Marissa, Schlievert, Patrick M, Herzberg, Mark C
Format:	Artikel
Sprache:	eng
Schlagworte:	5'-Nucleotidase - genetics 5'-Nucleotidase - metabolism Adenosine Adenosine - metabolism Adenosine triphosphate Adenosine Triphosphate - metabolism Agglomeration Animals Antibiotics Antigens ATP ATP (Adenosine triphosphate) Bacteria Bacterial Adhesion - genetics Bacterial infections Bacterial Proteins - genetics Bacterial Proteins - metabolism Biofilms Biology Cell surface Cell Wall - enzymology Clonal deletion Cloning Colorimetry Cytokines Deletion mutant Dentistry Electrophoresis, Polyacrylamide Gel Endocarditis Endocarditis, Bacterial - etiology Enzymes Escherichia Genomes Gram-positive bacteria Helicobacter pylori Hydrolysis Hydroxyapatite Identification Immunosuppression Infections Inflammation Kinetics Lag time Macrophages Mass Spectrometry Medicine Microbial Viability - genetics Microbicides Molecular Sequence Data Monocytes Muscle proteins Mutation Nucleotidase Nucleotidases Phagocytes Phosphatase Phosphates Platelet Adhesiveness Platelet Aggregation Proteins Rabbits Response time Staphylococcus aureus Streptococcal Infections - complications Streptococcal Infections - microbiology Streptococcus Streptococcus gordonii Streptococcus infections Streptococcus mitis Streptococcus oralis Streptococcus sanguis Streptococcus sanguis - enzymology Streptococcus sanguis - genetics Streptococcus sanguis - pathogenicity Vectors (Biology) Virulence Virulence - genetics Virulence factors Virulence Factors - genetics Virulence Factors - metabolism
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Zusammenfassung:	Streptococcus sanguinis is the most common cause of infective endocarditis (IE). Since the molecular basis of virulence of this oral commensal bacterium remains unclear, we searched the genome of S. sanguinis for previously unidentified virulence factors. We identified a cell surface ecto-5'-nucleotidase (Nt5e), as a candidate virulence factor. By colorimetric phosphate assay, we showed that S. sanguinis Nt5e can hydrolyze extracellular adenosine triphosphate to generate adenosine. Moreover, a nt5e deletion mutant showed significantly shorter lag time (P
ISSN:	1932-6203 1932-6203
DOI:	10.1371/journal.pone.0038059