Thermostable DNA polymerase from a viral metagenome is a potent RT-PCR enzyme

Viral metagenomic libraries are a promising but previously untapped source of new reagent enzymes. Deep sequencing and functional screening of viral metagenomic DNA from a near-boiling thermal pool identified clones expressing thermostable DNA polymerase (Pol) activity. Among these, 3173 Pol demonst...

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Veröffentlicht in:	PloS one 2012-06, Vol.7 (6), p.e38371
Hauptverfasser:	Moser, Michael J, DiFrancesco, Robert A, Gowda, Krishne, Klingele, Audrey J, Sugar, Darby R, Stocki, Stacy, Mead, David A, Schoenfeld, Thomas W
Format:	Artikel
Sprache:	eng
Schlagworte:	Bacillus Bias Biochemistry Biology Boiling Cloning Comparative analysis Deoxyribonucleic acid DNA DNA polymerase DNA polymerases DNA sequencing DNA-directed DNA polymerase DNA-Directed DNA Polymerase - metabolism Enzyme Stability Enzymes Exonuclease Gene expression Gene Library Gene sequencing Hot Springs - virology Humans Influenza A Influenza A virus - genetics Influenza A virus - isolation & purification Kinases Levivirus - genetics Levivirus - isolation & purification Ligands Medicine Metagenome - genetics Polymerase chain reaction Proofreading Proteins Reverse Transcriptase Polymerase Chain Reaction - methods Reverse transcription Ribonucleic acid RNA RNA, Messenger - genetics RNA, Messenger - metabolism RNA-directed DNA polymerase Senescence Stem cells Temperature Thermal stability Viruses - genetics
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