A LOV protein modulates the physiological attributes of Xanthomonas axonopodis pv. citri relevant for host plant colonization
Recent studies have demonstrated that an appropriate light environment is required for the establishment of efficient vegetal resistance responses in several plant-pathogen interactions. The photoreceptors implicated in such responses are mainly those belonging to the phytochrome family. Data obtain...
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| Veröffentlicht in: | PloS one 2012-06, Vol.7 (6), p.e38226 |
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| description | Recent studies have demonstrated that an appropriate light environment is required for the establishment of efficient vegetal resistance responses in several plant-pathogen interactions. The photoreceptors implicated in such responses are mainly those belonging to the phytochrome family. Data obtained from bacterial genome sequences revealed the presence of photosensory proteins of the BLUF (Blue Light sensing Using FAD), LOV (Light, Oxygen, Voltage) and phytochrome families with no known functions. Xanthomonas axonopodis pv. citri is a Gram-negative bacterium responsible for citrus canker. The in silico analysis of the X. axonopodis pv. citri genome sequence revealed the presence of a gene encoding a putative LOV photoreceptor, in addition to two genes encoding BLUF proteins. This suggests that blue light sensing could play a role in X. axonopodis pv. citri physiology. We obtained the recombinant Xac-LOV protein by expression in Escherichia coli and performed a spectroscopic analysis of the purified protein, which demonstrated that it has a canonical LOV photochemistry. We also constructed a mutant strain of X. axonopodis pv. citri lacking the LOV protein and found that the loss of this protein altered bacterial motility, exopolysaccharide production and biofilm formation. Moreover, we observed that the adhesion of the mutant strain to abiotic and biotic surfaces was significantly diminished compared to the wild-type. Finally, inoculation of orange (Citrus sinensis) leaves with the mutant strain of X. axonopodis pv. citri resulted in marked differences in the development of symptoms in plant tissues relative to the wild-type, suggesting a role for the Xac-LOV protein in the pathogenic process. Altogether, these results suggest the novel involvement of a photosensory system in the regulation of physiological attributes of a phytopathogenic bacterium. A functional blue light receptor in Xanthomonas spp. has been described for the first time, showing an important role in virulence during citrus canker disease. |
| doi_str_mv | 10.1371/journal.pone.0038226 |
| format | Article |
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The photoreceptors implicated in such responses are mainly those belonging to the phytochrome family. Data obtained from bacterial genome sequences revealed the presence of photosensory proteins of the BLUF (Blue Light sensing Using FAD), LOV (Light, Oxygen, Voltage) and phytochrome families with no known functions. Xanthomonas axonopodis pv. citri is a Gram-negative bacterium responsible for citrus canker. The in silico analysis of the X. axonopodis pv. citri genome sequence revealed the presence of a gene encoding a putative LOV photoreceptor, in addition to two genes encoding BLUF proteins. This suggests that blue light sensing could play a role in X. axonopodis pv. citri physiology. We obtained the recombinant Xac-LOV protein by expression in Escherichia coli and performed a spectroscopic analysis of the purified protein, which demonstrated that it has a canonical LOV photochemistry. We also constructed a mutant strain of X. axonopodis pv. citri lacking the LOV protein and found that the loss of this protein altered bacterial motility, exopolysaccharide production and biofilm formation. Moreover, we observed that the adhesion of the mutant strain to abiotic and biotic surfaces was significantly diminished compared to the wild-type. Finally, inoculation of orange (Citrus sinensis) leaves with the mutant strain of X. axonopodis pv. citri resulted in marked differences in the development of symptoms in plant tissues relative to the wild-type, suggesting a role for the Xac-LOV protein in the pathogenic process. Altogether, these results suggest the novel involvement of a photosensory system in the regulation of physiological attributes of a phytopathogenic bacterium. A functional blue light receptor in Xanthomonas spp. has been described for the first time, showing an important role in virulence during citrus canker disease.</description><identifier>ISSN: 1932-6203</identifier><identifier>EISSN: 1932-6203</identifier><identifier>DOI: 10.1371/journal.pone.0038226</identifier><identifier>PMID: 22675525</identifier><language>eng</language><publisher>United States: Public Library of Science</publisher><subject>Amino Acid Sequence ; Amino acids ; Analysis ; Antibiotics ; Bacillus subtilis ; Bacteria ; Bacterial Adhesion ; Bacterial Proteins - chemistry ; Bacterial Proteins - metabolism ; Biofilms ; Biology ; Canker ; Chromophores ; Citrus ; Citrus sinensis ; Citrus sinensis - microbiology ; Cloning ; Colonization ; Colony Count, Microbial ; Computational Biology ; E coli ; Gene Deletion ; Gene sequencing ; Genes, Bacterial - genetics ; Genetic engineering ; Genomes ; Genomics ; Gram-negative bacteria ; Histidine Kinase ; Host plants ; Host-Pathogen Interactions - physiology ; Inoculation ; Laboratories ; Leaves ; Light ; LOV protein ; Microbial drug resistance ; Microorganisms ; Molecular biology ; Molecular Sequence Data ; Motility ; Movement - physiology ; Mutagenesis ; Nucleotide sequence ; Oxygen ; Pathogens ; Photochemical Processes ; Photochemistry ; Photoreceptors ; Physiological aspects ; Physiology ; Phytochrome ; Plant Diseases - microbiology ; Plant Leaves - microbiology ; Plant tissues ; Plasmids ; Polysaccharides, Bacterial - biosynthesis ; Protein Kinases - metabolism ; Proteins ; Recombinant Proteins - metabolism ; Spectroscopic analysis ; Vectors (Biology) ; Virulence ; Virulence (Microbiology) ; Xanthomonas axonopodis - enzymology ; Xanthomonas axonopodis - genetics ; Xanthomonas axonopodis - growth & development ; Xanthomonas axonopodis - physiology ; Xanthomonas campestris</subject><ispartof>PloS one, 2012-06, Vol.7 (6), p.e38226</ispartof><rights>COPYRIGHT 2012 Public Library of Science</rights><rights>2012 Kraiselburd et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License: https://creativecommons.org/licenses/by/4.0/ (the “License”), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><rights>Kraiselburd et al. 2012</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c692t-600c74260ba5a44bd492e72ee71092da0684de766471be33953028a5057b22623</citedby><cites>FETCH-LOGICAL-c692t-600c74260ba5a44bd492e72ee71092da0684de766471be33953028a5057b22623</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC3366940/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC3366940/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,723,776,780,860,881,2096,2915,23845,27901,27902,53766,53768,79342,79343</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/22675525$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Kraiselburd, Ivana</creatorcontrib><creatorcontrib>Alet, Analía I</creatorcontrib><creatorcontrib>Tondo, María Laura</creatorcontrib><creatorcontrib>Petrocelli, Silvana</creatorcontrib><creatorcontrib>Daurelio, Lucas D</creatorcontrib><creatorcontrib>Monzón, Jesica</creatorcontrib><creatorcontrib>Ruiz, Oscar A</creatorcontrib><creatorcontrib>Losi, Aba</creatorcontrib><creatorcontrib>Orellano, Elena G</creatorcontrib><title>A LOV protein modulates the physiological attributes of Xanthomonas axonopodis pv. citri relevant for host plant colonization</title><title>PloS one</title><addtitle>PLoS One</addtitle><description>Recent studies have demonstrated that an appropriate light environment is required for the establishment of efficient vegetal resistance responses in several plant-pathogen interactions. The photoreceptors implicated in such responses are mainly those belonging to the phytochrome family. Data obtained from bacterial genome sequences revealed the presence of photosensory proteins of the BLUF (Blue Light sensing Using FAD), LOV (Light, Oxygen, Voltage) and phytochrome families with no known functions. Xanthomonas axonopodis pv. citri is a Gram-negative bacterium responsible for citrus canker. The in silico analysis of the X. axonopodis pv. citri genome sequence revealed the presence of a gene encoding a putative LOV photoreceptor, in addition to two genes encoding BLUF proteins. This suggests that blue light sensing could play a role in X. axonopodis pv. citri physiology. We obtained the recombinant Xac-LOV protein by expression in Escherichia coli and performed a spectroscopic analysis of the purified protein, which demonstrated that it has a canonical LOV photochemistry. We also constructed a mutant strain of X. axonopodis pv. citri lacking the LOV protein and found that the loss of this protein altered bacterial motility, exopolysaccharide production and biofilm formation. Moreover, we observed that the adhesion of the mutant strain to abiotic and biotic surfaces was significantly diminished compared to the wild-type. Finally, inoculation of orange (Citrus sinensis) leaves with the mutant strain of X. axonopodis pv. citri resulted in marked differences in the development of symptoms in plant tissues relative to the wild-type, suggesting a role for the Xac-LOV protein in the pathogenic process. Altogether, these results suggest the novel involvement of a photosensory system in the regulation of physiological attributes of a phytopathogenic bacterium. A functional blue light receptor in Xanthomonas spp. has been described for the first time, showing an important role in virulence during citrus canker disease.</description><subject>Amino Acid Sequence</subject><subject>Amino acids</subject><subject>Analysis</subject><subject>Antibiotics</subject><subject>Bacillus subtilis</subject><subject>Bacteria</subject><subject>Bacterial Adhesion</subject><subject>Bacterial Proteins - chemistry</subject><subject>Bacterial Proteins - metabolism</subject><subject>Biofilms</subject><subject>Biology</subject><subject>Canker</subject><subject>Chromophores</subject><subject>Citrus</subject><subject>Citrus sinensis</subject><subject>Citrus sinensis - microbiology</subject><subject>Cloning</subject><subject>Colonization</subject><subject>Colony Count, Microbial</subject><subject>Computational Biology</subject><subject>E coli</subject><subject>Gene Deletion</subject><subject>Gene sequencing</subject><subject>Genes, Bacterial - genetics</subject><subject>Genetic engineering</subject><subject>Genomes</subject><subject>Genomics</subject><subject>Gram-negative bacteria</subject><subject>Histidine Kinase</subject><subject>Host plants</subject><subject>Host-Pathogen Interactions - physiology</subject><subject>Inoculation</subject><subject>Laboratories</subject><subject>Leaves</subject><subject>Light</subject><subject>LOV protein</subject><subject>Microbial drug resistance</subject><subject>Microorganisms</subject><subject>Molecular biology</subject><subject>Molecular Sequence Data</subject><subject>Motility</subject><subject>Movement - physiology</subject><subject>Mutagenesis</subject><subject>Nucleotide sequence</subject><subject>Oxygen</subject><subject>Pathogens</subject><subject>Photochemical Processes</subject><subject>Photochemistry</subject><subject>Photoreceptors</subject><subject>Physiological aspects</subject><subject>Physiology</subject><subject>Phytochrome</subject><subject>Plant Diseases - microbiology</subject><subject>Plant Leaves - microbiology</subject><subject>Plant tissues</subject><subject>Plasmids</subject><subject>Polysaccharides, Bacterial - biosynthesis</subject><subject>Protein Kinases - metabolism</subject><subject>Proteins</subject><subject>Recombinant Proteins - metabolism</subject><subject>Spectroscopic analysis</subject><subject>Vectors (Biology)</subject><subject>Virulence</subject><subject>Virulence (Microbiology)</subject><subject>Xanthomonas axonopodis - enzymology</subject><subject>Xanthomonas axonopodis - genetics</subject><subject>Xanthomonas axonopodis - growth & development</subject><subject>Xanthomonas axonopodis - physiology</subject><subject>Xanthomonas campestris</subject><issn>1932-6203</issn><issn>1932-6203</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2012</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>BENPR</sourceid><sourceid>DOA</sourceid><recordid>eNqNk12L1DAUhoso7rr6D0QDgujFjPlo0-mNMCx-DCws-LF4F9L0dJol01OTdNkV_O9mnO4yI3shuWiaPOc9OW9ysuw5o3MmSvbuEkffazcfsIc5pWLBuXyQHbNK8JnkVDzcmx9lT0K4pLQQCykfZ0cJLYuCF8fZ7yU5O78gg8cIticbbEanIwQSOyBDdxMsOlxbox3RMXpbj9tNbMkP3ccON9jrQPQ19jhgYwMZrubE2AQSDw6uEkRa9KTDEMngtr8mCfb2l44W-6fZo1a7AM-m70n2_eOHb6efZ2fnn1any7OZkRWPM0mpKXMuaa0Lned1k1ccSg5QMlrxRlO5yBsopcxLVoMQVSEoX-iCFmWdSuXiJHu50x0cBjU5FxQTvEjOMZknYrUjGtSXavB2o_2NQm3V3wX0a6V9tMaBakG2pmKVMQLytuGalyBqVmtRLVg6RNJ6P2Ub6w00BvrotTsQPdzpbafWeKWEkLLKaRJ4Mwl4_DlCiGpjgwGX_AMc07kpq9K18rJK6Kt_0Purm6i1TgXYvsWU12xF1TIvS8ZkSpyo-T1UGg1srEnPrLVp_SDg7UFAYiJcx7UeQ1Crr1_-nz2_OGRf77EdaBe7gG7cPplwCOY70HgMwUN7ZzKjatslt26obZeoqUtS2Iv9C7oLum0L8Qdn3Q2k</recordid><startdate>20120604</startdate><enddate>20120604</enddate><creator>Kraiselburd, Ivana</creator><creator>Alet, Analía I</creator><creator>Tondo, María Laura</creator><creator>Petrocelli, Silvana</creator><creator>Daurelio, Lucas D</creator><creator>Monzón, Jesica</creator><creator>Ruiz, Oscar A</creator><creator>Losi, Aba</creator><creator>Orellano, Elena G</creator><general>Public Library of Science</general><general>Public Library of Science (PLoS)</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>IOV</scope><scope>ISR</scope><scope>3V.</scope><scope>7QG</scope><scope>7QL</scope><scope>7QO</scope><scope>7RV</scope><scope>7SN</scope><scope>7SS</scope><scope>7T5</scope><scope>7TG</scope><scope>7TM</scope><scope>7U9</scope><scope>7X2</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>8AO</scope><scope>8C1</scope><scope>8FD</scope><scope>8FE</scope><scope>8FG</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABJCF</scope><scope>ABUWG</scope><scope>AEUYN</scope><scope>AFKRA</scope><scope>ARAPS</scope><scope>ATCPS</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BGLVJ</scope><scope>BHPHI</scope><scope>C1K</scope><scope>CCPQU</scope><scope>D1I</scope><scope>DWQXO</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>H94</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>KB.</scope><scope>KB0</scope><scope>KL.</scope><scope>L6V</scope><scope>LK8</scope><scope>M0K</scope><scope>M0S</scope><scope>M1P</scope><scope>M7N</scope><scope>M7P</scope><scope>M7S</scope><scope>NAPCQ</scope><scope>P5Z</scope><scope>P62</scope><scope>P64</scope><scope>PATMY</scope><scope>PDBOC</scope><scope>PIMPY</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>PTHSS</scope><scope>PYCSY</scope><scope>RC3</scope><scope>7X8</scope><scope>5PM</scope><scope>DOA</scope></search><sort><creationdate>20120604</creationdate><title>A LOV protein modulates the physiological attributes of Xanthomonas axonopodis pv. citri relevant for host plant colonization</title><author>Kraiselburd, Ivana ; Alet, Analía I ; Tondo, María Laura ; Petrocelli, Silvana ; Daurelio, Lucas D ; Monzón, Jesica ; Ruiz, Oscar A ; Losi, Aba ; Orellano, Elena G</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c692t-600c74260ba5a44bd492e72ee71092da0684de766471be33953028a5057b22623</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2012</creationdate><topic>Amino Acid Sequence</topic><topic>Amino acids</topic><topic>Analysis</topic><topic>Antibiotics</topic><topic>Bacillus subtilis</topic><topic>Bacteria</topic><topic>Bacterial Adhesion</topic><topic>Bacterial Proteins - 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Academic</collection><collection>ProQuest Engineering Collection</collection><collection>ProQuest Biological Science Collection</collection><collection>Agricultural Science Database</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biological Science Database</collection><collection>Engineering Database</collection><collection>Nursing & Allied Health Premium</collection><collection>Advanced Technologies & Aerospace Database</collection><collection>ProQuest Advanced Technologies & Aerospace Collection</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Environmental Science Database</collection><collection>Materials Science Collection</collection><collection>Publicly Available Content Database</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><collection>Engineering Collection</collection><collection>Environmental Science Collection</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><collection>DOAJ Directory of Open Access Journals</collection><jtitle>PloS one</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Kraiselburd, Ivana</au><au>Alet, Analía I</au><au>Tondo, María Laura</au><au>Petrocelli, Silvana</au><au>Daurelio, Lucas D</au><au>Monzón, Jesica</au><au>Ruiz, Oscar A</au><au>Losi, Aba</au><au>Orellano, Elena G</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>A LOV protein modulates the physiological attributes of Xanthomonas axonopodis pv. citri relevant for host plant colonization</atitle><jtitle>PloS one</jtitle><addtitle>PLoS One</addtitle><date>2012-06-04</date><risdate>2012</risdate><volume>7</volume><issue>6</issue><spage>e38226</spage><pages>e38226-</pages><issn>1932-6203</issn><eissn>1932-6203</eissn><abstract>Recent studies have demonstrated that an appropriate light environment is required for the establishment of efficient vegetal resistance responses in several plant-pathogen interactions. The photoreceptors implicated in such responses are mainly those belonging to the phytochrome family. Data obtained from bacterial genome sequences revealed the presence of photosensory proteins of the BLUF (Blue Light sensing Using FAD), LOV (Light, Oxygen, Voltage) and phytochrome families with no known functions. Xanthomonas axonopodis pv. citri is a Gram-negative bacterium responsible for citrus canker. The in silico analysis of the X. axonopodis pv. citri genome sequence revealed the presence of a gene encoding a putative LOV photoreceptor, in addition to two genes encoding BLUF proteins. This suggests that blue light sensing could play a role in X. axonopodis pv. citri physiology. We obtained the recombinant Xac-LOV protein by expression in Escherichia coli and performed a spectroscopic analysis of the purified protein, which demonstrated that it has a canonical LOV photochemistry. We also constructed a mutant strain of X. axonopodis pv. citri lacking the LOV protein and found that the loss of this protein altered bacterial motility, exopolysaccharide production and biofilm formation. Moreover, we observed that the adhesion of the mutant strain to abiotic and biotic surfaces was significantly diminished compared to the wild-type. Finally, inoculation of orange (Citrus sinensis) leaves with the mutant strain of X. axonopodis pv. citri resulted in marked differences in the development of symptoms in plant tissues relative to the wild-type, suggesting a role for the Xac-LOV protein in the pathogenic process. Altogether, these results suggest the novel involvement of a photosensory system in the regulation of physiological attributes of a phytopathogenic bacterium. A functional blue light receptor in Xanthomonas spp. has been described for the first time, showing an important role in virulence during citrus canker disease.</abstract><cop>United States</cop><pub>Public Library of Science</pub><pmid>22675525</pmid><doi>10.1371/journal.pone.0038226</doi><tpages>e38226</tpages><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 1932-6203 |
| ispartof | PloS one, 2012-06, Vol.7 (6), p.e38226 |
| issn | 1932-6203 1932-6203 |
| language | eng |
| recordid | cdi_plos_journals_1325003164 |
| source | MEDLINE; DOAJ Directory of Open Access Journals; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; PubMed Central; Free Full-Text Journals in Chemistry; Public Library of Science (PLoS) |
| subjects | Amino Acid Sequence Amino acids Analysis Antibiotics Bacillus subtilis Bacteria Bacterial Adhesion Bacterial Proteins - chemistry Bacterial Proteins - metabolism Biofilms Biology Canker Chromophores Citrus Citrus sinensis Citrus sinensis - microbiology Cloning Colonization Colony Count, Microbial Computational Biology E coli Gene Deletion Gene sequencing Genes, Bacterial - genetics Genetic engineering Genomes Genomics Gram-negative bacteria Histidine Kinase Host plants Host-Pathogen Interactions - physiology Inoculation Laboratories Leaves Light LOV protein Microbial drug resistance Microorganisms Molecular biology Molecular Sequence Data Motility Movement - physiology Mutagenesis Nucleotide sequence Oxygen Pathogens Photochemical Processes Photochemistry Photoreceptors Physiological aspects Physiology Phytochrome Plant Diseases - microbiology Plant Leaves - microbiology Plant tissues Plasmids Polysaccharides, Bacterial - biosynthesis Protein Kinases - metabolism Proteins Recombinant Proteins - metabolism Spectroscopic analysis Vectors (Biology) Virulence Virulence (Microbiology) Xanthomonas axonopodis - enzymology Xanthomonas axonopodis - genetics Xanthomonas axonopodis - growth & development Xanthomonas axonopodis - physiology Xanthomonas campestris |
| title | A LOV protein modulates the physiological attributes of Xanthomonas axonopodis pv. citri relevant for host plant colonization |
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