A cytochrome p450 conserved in insects is involved in cuticle formation
The sequencing of numerous insect genomes has revealed dynamic changes in the number and identity of cytochrome P450 genes in different insects. In the evolutionary sense, the rapid birth and death of many P450 genes is observed, with only a small number of P450 genes showing orthology between insec...
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| Veröffentlicht in: | PloS one 2012-05, Vol.7 (5), p.e36544 |
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| creator | Sztal, Tamar Chung, Henry Berger, Silke Currie, Peter D Batterham, Philip Daborn, Phillip J |
| description | The sequencing of numerous insect genomes has revealed dynamic changes in the number and identity of cytochrome P450 genes in different insects. In the evolutionary sense, the rapid birth and death of many P450 genes is observed, with only a small number of P450 genes showing orthology between insects with sequenced genomes. It is likely that these conserved P450s function in conserved pathways. In this study, we demonstrate the P450 gene, Cyp301a1, present in all insect genomes sequenced to date, affects the formation of the adult cuticle in Drosophila melanogaster. A Cyp301a1 piggyBac insertion mutant and RNAi of Cyp301a1 both show a similar cuticle malformation phenotype, which can be reduced by 20-hydroxyecdysone, suggesting that Cyp301a1 is an important gene involved in the formation of the adult cuticle and may be involved in ecdysone regulation in this tissue. |
| doi_str_mv | 10.1371/journal.pone.0036544 |
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In the evolutionary sense, the rapid birth and death of many P450 genes is observed, with only a small number of P450 genes showing orthology between insects with sequenced genomes. It is likely that these conserved P450s function in conserved pathways. In this study, we demonstrate the P450 gene, Cyp301a1, present in all insect genomes sequenced to date, affects the formation of the adult cuticle in Drosophila melanogaster. A Cyp301a1 piggyBac insertion mutant and RNAi of Cyp301a1 both show a similar cuticle malformation phenotype, which can be reduced by 20-hydroxyecdysone, suggesting that Cyp301a1 is an important gene involved in the formation of the adult cuticle and may be involved in ecdysone regulation in this tissue.</description><identifier>ISSN: 1932-6203</identifier><identifier>EISSN: 1932-6203</identifier><identifier>DOI: 10.1371/journal.pone.0036544</identifier><identifier>PMID: 22574182</identifier><language>eng</language><publisher>United States: Public Library of Science</publisher><subject>Abdomen ; Amino Acid Sequence ; Animals ; Apoptosis ; Base Sequence ; Biochemistry ; Biological evolution ; Biology ; Biosynthesis ; Biotechnology ; Cloning ; Conserved Sequence ; Cuticles ; Cytochrome ; Cytochrome P-450 Enzyme System - chemistry ; Cytochrome P-450 Enzyme System - deficiency ; Cytochrome P-450 Enzyme System - genetics ; Cytochrome P-450 Enzyme System - metabolism ; Cytochrome P450 ; Drosophila ; Drosophila melanogaster ; Drosophila melanogaster - anatomy & histology ; Drosophila melanogaster - drug effects ; Drosophila melanogaster - enzymology ; Drosophila melanogaster - growth & development ; Drosophila Proteins - chemistry ; Drosophila Proteins - deficiency ; Drosophila Proteins - genetics ; Drosophila Proteins - metabolism ; Ecdysone ; Ecdysterone - pharmacology ; Enzymes ; Epidermis - abnormalities ; Epidermis - drug effects ; Epidermis - enzymology ; Epidermis - growth & development ; Evolution, Molecular ; Female ; Gene Expression Regulation, Developmental - drug effects ; Gene Knockdown Techniques ; Gene sequencing ; Genes ; Genome, Insect - genetics ; Genomes ; Genomics ; Halloween ; Insects ; Male ; Manduca sexta ; Metabolism ; Mutagenesis, Insertional ; Orthology ; Phenotype ; RNA Interference ; RNA-mediated interference ; Science ; Time Factors</subject><ispartof>PloS one, 2012-05, Vol.7 (5), p.e36544</ispartof><rights>COPYRIGHT 2012 Public Library of Science</rights><rights>2012 Sztal et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License: https://creativecommons.org/licenses/by/4.0/ (the “License”), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><rights>Sztal et al. 2012</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c692t-4e47922d3bfddac6817f48e5b91696203f95f9da2283f8dad1ff7d788086fe403</citedby><cites>FETCH-LOGICAL-c692t-4e47922d3bfddac6817f48e5b91696203f95f9da2283f8dad1ff7d788086fe403</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC3344891/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC3344891/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,723,776,780,860,881,2096,2915,23845,27901,27902,53766,53768,79343,79344</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/22574182$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><contributor>Dearden, Peter K.</contributor><creatorcontrib>Sztal, Tamar</creatorcontrib><creatorcontrib>Chung, Henry</creatorcontrib><creatorcontrib>Berger, Silke</creatorcontrib><creatorcontrib>Currie, Peter D</creatorcontrib><creatorcontrib>Batterham, Philip</creatorcontrib><creatorcontrib>Daborn, Phillip J</creatorcontrib><title>A cytochrome p450 conserved in insects is involved in cuticle formation</title><title>PloS one</title><addtitle>PLoS One</addtitle><description>The sequencing of numerous insect genomes has revealed dynamic changes in the number and identity of cytochrome P450 genes in different insects. In the evolutionary sense, the rapid birth and death of many P450 genes is observed, with only a small number of P450 genes showing orthology between insects with sequenced genomes. It is likely that these conserved P450s function in conserved pathways. In this study, we demonstrate the P450 gene, Cyp301a1, present in all insect genomes sequenced to date, affects the formation of the adult cuticle in Drosophila melanogaster. A Cyp301a1 piggyBac insertion mutant and RNAi of Cyp301a1 both show a similar cuticle malformation phenotype, which can be reduced by 20-hydroxyecdysone, suggesting that Cyp301a1 is an important gene involved in the formation of the adult cuticle and may be involved in ecdysone regulation in this tissue.</description><subject>Abdomen</subject><subject>Amino Acid Sequence</subject><subject>Animals</subject><subject>Apoptosis</subject><subject>Base Sequence</subject><subject>Biochemistry</subject><subject>Biological evolution</subject><subject>Biology</subject><subject>Biosynthesis</subject><subject>Biotechnology</subject><subject>Cloning</subject><subject>Conserved Sequence</subject><subject>Cuticles</subject><subject>Cytochrome</subject><subject>Cytochrome P-450 Enzyme System - chemistry</subject><subject>Cytochrome P-450 Enzyme System - deficiency</subject><subject>Cytochrome P-450 Enzyme System - genetics</subject><subject>Cytochrome P-450 Enzyme System - 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cytochrome p450 conserved in insects is involved in cuticle formation</title><author>Sztal, Tamar ; Chung, Henry ; Berger, Silke ; Currie, Peter D ; Batterham, Philip ; Daborn, Phillip J</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c692t-4e47922d3bfddac6817f48e5b91696203f95f9da2283f8dad1ff7d788086fe403</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2012</creationdate><topic>Abdomen</topic><topic>Amino Acid Sequence</topic><topic>Animals</topic><topic>Apoptosis</topic><topic>Base Sequence</topic><topic>Biochemistry</topic><topic>Biological evolution</topic><topic>Biology</topic><topic>Biosynthesis</topic><topic>Biotechnology</topic><topic>Cloning</topic><topic>Conserved Sequence</topic><topic>Cuticles</topic><topic>Cytochrome</topic><topic>Cytochrome P-450 Enzyme System - chemistry</topic><topic>Cytochrome P-450 Enzyme System - deficiency</topic><topic>Cytochrome P-450 Enzyme System - genetics</topic><topic>Cytochrome P-450 Enzyme System - metabolism</topic><topic>Cytochrome P450</topic><topic>Drosophila</topic><topic>Drosophila melanogaster</topic><topic>Drosophila melanogaster - anatomy & histology</topic><topic>Drosophila melanogaster - drug effects</topic><topic>Drosophila melanogaster - enzymology</topic><topic>Drosophila melanogaster - growth & development</topic><topic>Drosophila Proteins - chemistry</topic><topic>Drosophila Proteins - deficiency</topic><topic>Drosophila Proteins - genetics</topic><topic>Drosophila Proteins - metabolism</topic><topic>Ecdysone</topic><topic>Ecdysterone - pharmacology</topic><topic>Enzymes</topic><topic>Epidermis - abnormalities</topic><topic>Epidermis - drug effects</topic><topic>Epidermis - enzymology</topic><topic>Epidermis - growth & development</topic><topic>Evolution, Molecular</topic><topic>Female</topic><topic>Gene Expression Regulation, Developmental - drug effects</topic><topic>Gene Knockdown Techniques</topic><topic>Gene sequencing</topic><topic>Genes</topic><topic>Genome, Insect - genetics</topic><topic>Genomes</topic><topic>Genomics</topic><topic>Halloween</topic><topic>Insects</topic><topic>Male</topic><topic>Manduca sexta</topic><topic>Metabolism</topic><topic>Mutagenesis, Insertional</topic><topic>Orthology</topic><topic>Phenotype</topic><topic>RNA Interference</topic><topic>RNA-mediated interference</topic><topic>Science</topic><topic>Time Factors</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Sztal, Tamar</creatorcontrib><creatorcontrib>Chung, Henry</creatorcontrib><creatorcontrib>Berger, Silke</creatorcontrib><creatorcontrib>Currie, Peter D</creatorcontrib><creatorcontrib>Batterham, Philip</creatorcontrib><creatorcontrib>Daborn, Phillip J</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE 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| subjects | Abdomen Amino Acid Sequence Animals Apoptosis Base Sequence Biochemistry Biological evolution Biology Biosynthesis Biotechnology Cloning Conserved Sequence Cuticles Cytochrome Cytochrome P-450 Enzyme System - chemistry Cytochrome P-450 Enzyme System - deficiency Cytochrome P-450 Enzyme System - genetics Cytochrome P-450 Enzyme System - metabolism Cytochrome P450 Drosophila Drosophila melanogaster Drosophila melanogaster - anatomy & histology Drosophila melanogaster - drug effects Drosophila melanogaster - enzymology Drosophila melanogaster - growth & development Drosophila Proteins - chemistry Drosophila Proteins - deficiency Drosophila Proteins - genetics Drosophila Proteins - metabolism Ecdysone Ecdysterone - pharmacology Enzymes Epidermis - abnormalities Epidermis - drug effects Epidermis - enzymology Epidermis - growth & development Evolution, Molecular Female Gene Expression Regulation, Developmental - drug effects Gene Knockdown Techniques Gene sequencing Genes Genome, Insect - genetics Genomes Genomics Halloween Insects Male Manduca sexta Metabolism Mutagenesis, Insertional Orthology Phenotype RNA Interference RNA-mediated interference Science Time Factors |
| title | A cytochrome p450 conserved in insects is involved in cuticle formation |
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