A novel Escherichia coli O157:H7 clone causing a major hemolytic uremic syndrome outbreak in China
An Escherichia coli O157:H7 outbreak in China in 1999 caused 177 deaths due to hemolytic uremic syndrome. Sixteen outbreak associated isolates were found to belong to a new clone, sequence type 96 (ST96), based on multilocus sequence typing of 15 housekeeping genes. Whole genome sequencing of an out...
Gespeichert in:
| Veröffentlicht in: | PloS one 2012-04, Vol.7 (4), p.e36144-e36144 |
|---|---|
| Hauptverfasser: | , , , , , , , , , , , , , , , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | e36144 |
|---|---|
| container_issue | 4 |
| container_start_page | e36144 |
| container_title | PloS one |
| container_volume | 7 |
| creator | Xiong, Yanwen Wang, Ping Lan, Ruiting Ye, Changyun Wang, Hua Ren, Jun Jing, Huaiqi Wang, Yiting Zhou, Zhemin Bai, Xuemei Cui, Zhigang Luo, Xia Zhao, Ailan Wang, Yan Zhang, Shaomin Sun, Hui Wang, Lei Xu, Jianguo |
| description | An Escherichia coli O157:H7 outbreak in China in 1999 caused 177 deaths due to hemolytic uremic syndrome. Sixteen outbreak associated isolates were found to belong to a new clone, sequence type 96 (ST96), based on multilocus sequence typing of 15 housekeeping genes. Whole genome sequencing of an outbreak isolate, Xuzhou21, showed that the isolate is phylogenetically closely related to the Japan 1996 outbreak isolate Sakai, both of which share the most recent common ancestor with the US outbreak isolate EDL933. The levels of IL-6 and IL-8 of peripheral blood mononuclear cells induced by Xuzhou21 and Sakai were significantly higher than that induced by EDL933. Xuzhou21 also induced a significantly higher level of IL-8 than Sakai while both induced similar levels of IL-6. The expression level of Shiga toxin 2 in Xuzhou21 induced by mitomycin C was 68.6 times of that under non-inducing conditions, twice of that induced in Sakai (32.7 times) and 15 times higher than that induced in EDL933 (4.5 times). Our study shows that ST96 is a novel clone and provided significant new insights into the evolution of virulence of E. coli O157:H7. |
| doi_str_mv | 10.1371/journal.pone.0036144 |
| format | Article |
| fullrecord | <record><control><sourceid>gale_plos_</sourceid><recordid>TN_cdi_plos_journals_1324600174</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><galeid>A477034035</galeid><doaj_id>oai_doaj_org_article_9363875088b745fea927e4d69f572977</doaj_id><sourcerecordid>A477034035</sourcerecordid><originalsourceid>FETCH-LOGICAL-c758t-4f482bb4533d13793a524214878962f1fcb5ce3ebc3ad9668701a8dfc5b151e3</originalsourceid><addsrcrecordid>eNqNk9-L1DAQx4so3nn6H4gGBNGHXfOzaX0QluX0Fg4W9PA1pGm6zZome0l7uP-9qds7duUeJA8Jk898JzOTybLXCM4R4ejT1g_BSTvfeafnEJIcUfokO0clwbMcQ_L06HyWvYhxCyEjRZ4_z84wZqwgOTzPqgVw_k5bcBlVq4NRrZFAeWvAGjH--YoDZVMAoOQQjdsACTq59QG0uvN23xsFhqC7tMW9q4PvNPBDXwUtfwHjwLI1Tr7MnjXSRv1q2i-ym6-XN8ur2fX622q5uJ4pzop-Rhta4KqijJA6JVgSyTDFiBa8KHPcoEZVTGmiK0VkXeZ5wSGSRd0oViGGNLnI3h5kd9ZHMVUnCkQwzSFEnCZidSBqL7diF0wnw154acRfgw8bIUNKyWpRkpwUnMGiqDhljZYl5prWedkwjkvOk9aXKdpQdbpW2vVB2hPR0xtnWrHxd4IQUrCSJYEPk0Dwt4OOvehMVNpa6bQf0rshQmP_KE7ou3_Qx7ObqI1MCRjX-BRXjaJiQTmHhEIyhp0_QqVVj11MnW5Msp84fDxxSEyvf_eb9B-iWP34_v_s-ucp-_6IbbW0fRu9HXrjXTwF6QFUwccYdPNQZATFOAn31RDjJIhpEpLbm-MGPTjdf33yB2kmAH4</addsrcrecordid><sourcetype>Open Website</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>1324600174</pqid></control><display><type>article</type><title>A novel Escherichia coli O157:H7 clone causing a major hemolytic uremic syndrome outbreak in China</title><source>MEDLINE</source><source>DOAJ Directory of Open Access Journals</source><source>Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals</source><source>PubMed Central</source><source>Free Full-Text Journals in Chemistry</source><source>Public Library of Science (PLoS)</source><creator>Xiong, Yanwen ; Wang, Ping ; Lan, Ruiting ; Ye, Changyun ; Wang, Hua ; Ren, Jun ; Jing, Huaiqi ; Wang, Yiting ; Zhou, Zhemin ; Bai, Xuemei ; Cui, Zhigang ; Luo, Xia ; Zhao, Ailan ; Wang, Yan ; Zhang, Shaomin ; Sun, Hui ; Wang, Lei ; Xu, Jianguo</creator><contributor>Ravel, Jacques</contributor><creatorcontrib>Xiong, Yanwen ; Wang, Ping ; Lan, Ruiting ; Ye, Changyun ; Wang, Hua ; Ren, Jun ; Jing, Huaiqi ; Wang, Yiting ; Zhou, Zhemin ; Bai, Xuemei ; Cui, Zhigang ; Luo, Xia ; Zhao, Ailan ; Wang, Yan ; Zhang, Shaomin ; Sun, Hui ; Wang, Lei ; Xu, Jianguo ; Ravel, Jacques</creatorcontrib><description>An Escherichia coli O157:H7 outbreak in China in 1999 caused 177 deaths due to hemolytic uremic syndrome. Sixteen outbreak associated isolates were found to belong to a new clone, sequence type 96 (ST96), based on multilocus sequence typing of 15 housekeeping genes. Whole genome sequencing of an outbreak isolate, Xuzhou21, showed that the isolate is phylogenetically closely related to the Japan 1996 outbreak isolate Sakai, both of which share the most recent common ancestor with the US outbreak isolate EDL933. The levels of IL-6 and IL-8 of peripheral blood mononuclear cells induced by Xuzhou21 and Sakai were significantly higher than that induced by EDL933. Xuzhou21 also induced a significantly higher level of IL-8 than Sakai while both induced similar levels of IL-6. The expression level of Shiga toxin 2 in Xuzhou21 induced by mitomycin C was 68.6 times of that under non-inducing conditions, twice of that induced in Sakai (32.7 times) and 15 times higher than that induced in EDL933 (4.5 times). Our study shows that ST96 is a novel clone and provided significant new insights into the evolution of virulence of E. coli O157:H7.</description><identifier>ISSN: 1932-6203</identifier><identifier>EISSN: 1932-6203</identifier><identifier>DOI: 10.1371/journal.pone.0036144</identifier><identifier>PMID: 22558360</identifier><language>eng</language><publisher>United States: Public Library of Science</publisher><subject>Adolescent ; Adult ; Age Distribution ; Biology ; Chemokines ; China - epidemiology ; Cholera ; Cloning ; Cytokines ; Cytokines - metabolism ; Diarrhea ; Disease control ; Disease Outbreaks - statistics & numerical data ; Disease prevention ; DNA sequencing ; E coli ; Escherichia coli ; Escherichia coli O157 - genetics ; Escherichia coli O157 - isolation & purification ; Escherichia coli O157 - physiology ; Female ; Gene sequencing ; Genes ; Genes, Bacterial - genetics ; Genomes ; Genomics ; Geography ; Hemolytic uremic syndrome ; Hemolytic-Uremic Syndrome - epidemiology ; Hemolytic-Uremic Syndrome - microbiology ; Humans ; Infectious diseases ; Interleukin 6 ; Interleukin 8 ; Japan - epidemiology ; Laboratories ; Leukocytes (mononuclear) ; Life sciences ; Male ; Medicine ; Middle Aged ; Mitomycin ; Mitomycin C ; Molecular Sequence Data ; Multilocus sequence typing ; Nucleotide sequence ; Outbreaks ; Peripheral blood mononuclear cells ; Phylogeny ; Sequence Analysis, DNA ; Shiga toxin ; Shiga toxin 2 ; Shiga Toxin 2 - genetics ; Shiga Toxin 2 - metabolism ; Toxins ; Virulence ; Young Adult</subject><ispartof>PloS one, 2012-04, Vol.7 (4), p.e36144-e36144</ispartof><rights>COPYRIGHT 2012 Public Library of Science</rights><rights>2012 Xiong et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License: https://creativecommons.org/licenses/by/4.0/ (the “License”), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><rights>Xiong et al. 2012</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c758t-4f482bb4533d13793a524214878962f1fcb5ce3ebc3ad9668701a8dfc5b151e3</citedby><cites>FETCH-LOGICAL-c758t-4f482bb4533d13793a524214878962f1fcb5ce3ebc3ad9668701a8dfc5b151e3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC3338595/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC3338595/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,723,776,780,860,881,2096,2915,23845,27901,27902,53766,53768,79343,79344</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/22558360$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><contributor>Ravel, Jacques</contributor><creatorcontrib>Xiong, Yanwen</creatorcontrib><creatorcontrib>Wang, Ping</creatorcontrib><creatorcontrib>Lan, Ruiting</creatorcontrib><creatorcontrib>Ye, Changyun</creatorcontrib><creatorcontrib>Wang, Hua</creatorcontrib><creatorcontrib>Ren, Jun</creatorcontrib><creatorcontrib>Jing, Huaiqi</creatorcontrib><creatorcontrib>Wang, Yiting</creatorcontrib><creatorcontrib>Zhou, Zhemin</creatorcontrib><creatorcontrib>Bai, Xuemei</creatorcontrib><creatorcontrib>Cui, Zhigang</creatorcontrib><creatorcontrib>Luo, Xia</creatorcontrib><creatorcontrib>Zhao, Ailan</creatorcontrib><creatorcontrib>Wang, Yan</creatorcontrib><creatorcontrib>Zhang, Shaomin</creatorcontrib><creatorcontrib>Sun, Hui</creatorcontrib><creatorcontrib>Wang, Lei</creatorcontrib><creatorcontrib>Xu, Jianguo</creatorcontrib><title>A novel Escherichia coli O157:H7 clone causing a major hemolytic uremic syndrome outbreak in China</title><title>PloS one</title><addtitle>PLoS One</addtitle><description>An Escherichia coli O157:H7 outbreak in China in 1999 caused 177 deaths due to hemolytic uremic syndrome. Sixteen outbreak associated isolates were found to belong to a new clone, sequence type 96 (ST96), based on multilocus sequence typing of 15 housekeeping genes. Whole genome sequencing of an outbreak isolate, Xuzhou21, showed that the isolate is phylogenetically closely related to the Japan 1996 outbreak isolate Sakai, both of which share the most recent common ancestor with the US outbreak isolate EDL933. The levels of IL-6 and IL-8 of peripheral blood mononuclear cells induced by Xuzhou21 and Sakai were significantly higher than that induced by EDL933. Xuzhou21 also induced a significantly higher level of IL-8 than Sakai while both induced similar levels of IL-6. The expression level of Shiga toxin 2 in Xuzhou21 induced by mitomycin C was 68.6 times of that under non-inducing conditions, twice of that induced in Sakai (32.7 times) and 15 times higher than that induced in EDL933 (4.5 times). Our study shows that ST96 is a novel clone and provided significant new insights into the evolution of virulence of E. coli O157:H7.</description><subject>Adolescent</subject><subject>Adult</subject><subject>Age Distribution</subject><subject>Biology</subject><subject>Chemokines</subject><subject>China - epidemiology</subject><subject>Cholera</subject><subject>Cloning</subject><subject>Cytokines</subject><subject>Cytokines - metabolism</subject><subject>Diarrhea</subject><subject>Disease control</subject><subject>Disease Outbreaks - statistics & numerical data</subject><subject>Disease prevention</subject><subject>DNA sequencing</subject><subject>E coli</subject><subject>Escherichia coli</subject><subject>Escherichia coli O157 - genetics</subject><subject>Escherichia coli O157 - isolation & purification</subject><subject>Escherichia coli O157 - physiology</subject><subject>Female</subject><subject>Gene sequencing</subject><subject>Genes</subject><subject>Genes, Bacterial - genetics</subject><subject>Genomes</subject><subject>Genomics</subject><subject>Geography</subject><subject>Hemolytic uremic syndrome</subject><subject>Hemolytic-Uremic Syndrome - epidemiology</subject><subject>Hemolytic-Uremic Syndrome - microbiology</subject><subject>Humans</subject><subject>Infectious diseases</subject><subject>Interleukin 6</subject><subject>Interleukin 8</subject><subject>Japan - epidemiology</subject><subject>Laboratories</subject><subject>Leukocytes (mononuclear)</subject><subject>Life sciences</subject><subject>Male</subject><subject>Medicine</subject><subject>Middle Aged</subject><subject>Mitomycin</subject><subject>Mitomycin C</subject><subject>Molecular Sequence Data</subject><subject>Multilocus sequence typing</subject><subject>Nucleotide sequence</subject><subject>Outbreaks</subject><subject>Peripheral blood mononuclear cells</subject><subject>Phylogeny</subject><subject>Sequence Analysis, DNA</subject><subject>Shiga toxin</subject><subject>Shiga toxin 2</subject><subject>Shiga Toxin 2 - genetics</subject><subject>Shiga Toxin 2 - metabolism</subject><subject>Toxins</subject><subject>Virulence</subject><subject>Young Adult</subject><issn>1932-6203</issn><issn>1932-6203</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2012</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>BENPR</sourceid><sourceid>DOA</sourceid><recordid>eNqNk9-L1DAQx4so3nn6H4gGBNGHXfOzaX0QluX0Fg4W9PA1pGm6zZome0l7uP-9qds7duUeJA8Jk898JzOTybLXCM4R4ejT1g_BSTvfeafnEJIcUfokO0clwbMcQ_L06HyWvYhxCyEjRZ4_z84wZqwgOTzPqgVw_k5bcBlVq4NRrZFAeWvAGjH--YoDZVMAoOQQjdsACTq59QG0uvN23xsFhqC7tMW9q4PvNPBDXwUtfwHjwLI1Tr7MnjXSRv1q2i-ym6-XN8ur2fX622q5uJ4pzop-Rhta4KqijJA6JVgSyTDFiBa8KHPcoEZVTGmiK0VkXeZ5wSGSRd0oViGGNLnI3h5kd9ZHMVUnCkQwzSFEnCZidSBqL7diF0wnw154acRfgw8bIUNKyWpRkpwUnMGiqDhljZYl5prWedkwjkvOk9aXKdpQdbpW2vVB2hPR0xtnWrHxd4IQUrCSJYEPk0Dwt4OOvehMVNpa6bQf0rshQmP_KE7ou3_Qx7ObqI1MCRjX-BRXjaJiQTmHhEIyhp0_QqVVj11MnW5Msp84fDxxSEyvf_eb9B-iWP34_v_s-ucp-_6IbbW0fRu9HXrjXTwF6QFUwccYdPNQZATFOAn31RDjJIhpEpLbm-MGPTjdf33yB2kmAH4</recordid><startdate>20120427</startdate><enddate>20120427</enddate><creator>Xiong, Yanwen</creator><creator>Wang, Ping</creator><creator>Lan, Ruiting</creator><creator>Ye, Changyun</creator><creator>Wang, Hua</creator><creator>Ren, Jun</creator><creator>Jing, Huaiqi</creator><creator>Wang, Yiting</creator><creator>Zhou, Zhemin</creator><creator>Bai, Xuemei</creator><creator>Cui, Zhigang</creator><creator>Luo, Xia</creator><creator>Zhao, Ailan</creator><creator>Wang, Yan</creator><creator>Zhang, Shaomin</creator><creator>Sun, Hui</creator><creator>Wang, Lei</creator><creator>Xu, Jianguo</creator><general>Public Library of Science</general><general>Public Library of Science (PLoS)</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>IOV</scope><scope>ISR</scope><scope>3V.</scope><scope>7QG</scope><scope>7QL</scope><scope>7QO</scope><scope>7RV</scope><scope>7SN</scope><scope>7SS</scope><scope>7T5</scope><scope>7TG</scope><scope>7TM</scope><scope>7U9</scope><scope>7X2</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>8AO</scope><scope>8C1</scope><scope>8FD</scope><scope>8FE</scope><scope>8FG</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABJCF</scope><scope>ABUWG</scope><scope>AEUYN</scope><scope>AFKRA</scope><scope>ARAPS</scope><scope>ATCPS</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BGLVJ</scope><scope>BHPHI</scope><scope>C1K</scope><scope>CCPQU</scope><scope>D1I</scope><scope>DWQXO</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>H94</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>KB.</scope><scope>KB0</scope><scope>KL.</scope><scope>L6V</scope><scope>LK8</scope><scope>M0K</scope><scope>M0S</scope><scope>M1P</scope><scope>M7N</scope><scope>M7P</scope><scope>M7S</scope><scope>NAPCQ</scope><scope>P5Z</scope><scope>P62</scope><scope>P64</scope><scope>PATMY</scope><scope>PDBOC</scope><scope>PIMPY</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>PTHSS</scope><scope>PYCSY</scope><scope>RC3</scope><scope>7X8</scope><scope>5PM</scope><scope>DOA</scope></search><sort><creationdate>20120427</creationdate><title>A novel Escherichia coli O157:H7 clone causing a major hemolytic uremic syndrome outbreak in China</title><author>Xiong, Yanwen ; Wang, Ping ; Lan, Ruiting ; Ye, Changyun ; Wang, Hua ; Ren, Jun ; Jing, Huaiqi ; Wang, Yiting ; Zhou, Zhemin ; Bai, Xuemei ; Cui, Zhigang ; Luo, Xia ; Zhao, Ailan ; Wang, Yan ; Zhang, Shaomin ; Sun, Hui ; Wang, Lei ; Xu, Jianguo</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c758t-4f482bb4533d13793a524214878962f1fcb5ce3ebc3ad9668701a8dfc5b151e3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2012</creationdate><topic>Adolescent</topic><topic>Adult</topic><topic>Age Distribution</topic><topic>Biology</topic><topic>Chemokines</topic><topic>China - epidemiology</topic><topic>Cholera</topic><topic>Cloning</topic><topic>Cytokines</topic><topic>Cytokines - metabolism</topic><topic>Diarrhea</topic><topic>Disease control</topic><topic>Disease Outbreaks - statistics & numerical data</topic><topic>Disease prevention</topic><topic>DNA sequencing</topic><topic>E coli</topic><topic>Escherichia coli</topic><topic>Escherichia coli O157 - genetics</topic><topic>Escherichia coli O157 - isolation & purification</topic><topic>Escherichia coli O157 - physiology</topic><topic>Female</topic><topic>Gene sequencing</topic><topic>Genes</topic><topic>Genes, Bacterial - genetics</topic><topic>Genomes</topic><topic>Genomics</topic><topic>Geography</topic><topic>Hemolytic uremic syndrome</topic><topic>Hemolytic-Uremic Syndrome - epidemiology</topic><topic>Hemolytic-Uremic Syndrome - microbiology</topic><topic>Humans</topic><topic>Infectious diseases</topic><topic>Interleukin 6</topic><topic>Interleukin 8</topic><topic>Japan - epidemiology</topic><topic>Laboratories</topic><topic>Leukocytes (mononuclear)</topic><topic>Life sciences</topic><topic>Male</topic><topic>Medicine</topic><topic>Middle Aged</topic><topic>Mitomycin</topic><topic>Mitomycin C</topic><topic>Molecular Sequence Data</topic><topic>Multilocus sequence typing</topic><topic>Nucleotide sequence</topic><topic>Outbreaks</topic><topic>Peripheral blood mononuclear cells</topic><topic>Phylogeny</topic><topic>Sequence Analysis, DNA</topic><topic>Shiga toxin</topic><topic>Shiga toxin 2</topic><topic>Shiga Toxin 2 - genetics</topic><topic>Shiga Toxin 2 - metabolism</topic><topic>Toxins</topic><topic>Virulence</topic><topic>Young Adult</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Xiong, Yanwen</creatorcontrib><creatorcontrib>Wang, Ping</creatorcontrib><creatorcontrib>Lan, Ruiting</creatorcontrib><creatorcontrib>Ye, Changyun</creatorcontrib><creatorcontrib>Wang, Hua</creatorcontrib><creatorcontrib>Ren, Jun</creatorcontrib><creatorcontrib>Jing, Huaiqi</creatorcontrib><creatorcontrib>Wang, Yiting</creatorcontrib><creatorcontrib>Zhou, Zhemin</creatorcontrib><creatorcontrib>Bai, Xuemei</creatorcontrib><creatorcontrib>Cui, Zhigang</creatorcontrib><creatorcontrib>Luo, Xia</creatorcontrib><creatorcontrib>Zhao, Ailan</creatorcontrib><creatorcontrib>Wang, Yan</creatorcontrib><creatorcontrib>Zhang, Shaomin</creatorcontrib><creatorcontrib>Sun, Hui</creatorcontrib><creatorcontrib>Wang, Lei</creatorcontrib><creatorcontrib>Xu, Jianguo</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Gale In Context: Opposing Viewpoints</collection><collection>Gale In Context: Science</collection><collection>ProQuest Central (Corporate)</collection><collection>Animal Behavior Abstracts</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Biotechnology Research Abstracts</collection><collection>Nursing & Allied Health Database</collection><collection>Ecology Abstracts</collection><collection>Entomology Abstracts (Full archive)</collection><collection>Immunology Abstracts</collection><collection>Meteorological & Geoastrophysical Abstracts</collection><collection>Nucleic Acids Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>Agricultural Science Collection</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Medical Database (Alumni Edition)</collection><collection>ProQuest Pharma Collection</collection><collection>Public Health Database</collection><collection>Technology Research Database</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Technology Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>Materials Science & Engineering Collection</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest One Sustainability</collection><collection>ProQuest Central UK/Ireland</collection><collection>Advanced Technologies & Aerospace Collection</collection><collection>Agricultural & Environmental Science Collection</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Technology Collection</collection><collection>Natural Science Collection</collection><collection>Environmental Sciences and Pollution Management</collection><collection>ProQuest One Community College</collection><collection>ProQuest Materials Science Collection</collection><collection>ProQuest Central Korea</collection><collection>Engineering Research Database</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Materials Science Database</collection><collection>Nursing & Allied Health Database (Alumni Edition)</collection><collection>Meteorological & Geoastrophysical Abstracts - Academic</collection><collection>ProQuest Engineering Collection</collection><collection>ProQuest Biological Science Collection</collection><collection>Agricultural Science Database</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biological Science Database</collection><collection>Engineering Database</collection><collection>Nursing & Allied Health Premium</collection><collection>Advanced Technologies & Aerospace Database</collection><collection>ProQuest Advanced Technologies & Aerospace Collection</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Environmental Science Database</collection><collection>Materials Science Collection</collection><collection>Publicly Available Content Database</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><collection>Engineering Collection</collection><collection>Environmental Science Collection</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><collection>DOAJ Directory of Open Access Journals</collection><jtitle>PloS one</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Xiong, Yanwen</au><au>Wang, Ping</au><au>Lan, Ruiting</au><au>Ye, Changyun</au><au>Wang, Hua</au><au>Ren, Jun</au><au>Jing, Huaiqi</au><au>Wang, Yiting</au><au>Zhou, Zhemin</au><au>Bai, Xuemei</au><au>Cui, Zhigang</au><au>Luo, Xia</au><au>Zhao, Ailan</au><au>Wang, Yan</au><au>Zhang, Shaomin</au><au>Sun, Hui</au><au>Wang, Lei</au><au>Xu, Jianguo</au><au>Ravel, Jacques</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>A novel Escherichia coli O157:H7 clone causing a major hemolytic uremic syndrome outbreak in China</atitle><jtitle>PloS one</jtitle><addtitle>PLoS One</addtitle><date>2012-04-27</date><risdate>2012</risdate><volume>7</volume><issue>4</issue><spage>e36144</spage><epage>e36144</epage><pages>e36144-e36144</pages><issn>1932-6203</issn><eissn>1932-6203</eissn><abstract>An Escherichia coli O157:H7 outbreak in China in 1999 caused 177 deaths due to hemolytic uremic syndrome. Sixteen outbreak associated isolates were found to belong to a new clone, sequence type 96 (ST96), based on multilocus sequence typing of 15 housekeeping genes. Whole genome sequencing of an outbreak isolate, Xuzhou21, showed that the isolate is phylogenetically closely related to the Japan 1996 outbreak isolate Sakai, both of which share the most recent common ancestor with the US outbreak isolate EDL933. The levels of IL-6 and IL-8 of peripheral blood mononuclear cells induced by Xuzhou21 and Sakai were significantly higher than that induced by EDL933. Xuzhou21 also induced a significantly higher level of IL-8 than Sakai while both induced similar levels of IL-6. The expression level of Shiga toxin 2 in Xuzhou21 induced by mitomycin C was 68.6 times of that under non-inducing conditions, twice of that induced in Sakai (32.7 times) and 15 times higher than that induced in EDL933 (4.5 times). Our study shows that ST96 is a novel clone and provided significant new insights into the evolution of virulence of E. coli O157:H7.</abstract><cop>United States</cop><pub>Public Library of Science</pub><pmid>22558360</pmid><doi>10.1371/journal.pone.0036144</doi><tpages>e36144</tpages><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 1932-6203 |
| ispartof | PloS one, 2012-04, Vol.7 (4), p.e36144-e36144 |
| issn | 1932-6203 1932-6203 |
| language | eng |
| recordid | cdi_plos_journals_1324600174 |
| source | MEDLINE; DOAJ Directory of Open Access Journals; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; PubMed Central; Free Full-Text Journals in Chemistry; Public Library of Science (PLoS) |
| subjects | Adolescent Adult Age Distribution Biology Chemokines China - epidemiology Cholera Cloning Cytokines Cytokines - metabolism Diarrhea Disease control Disease Outbreaks - statistics & numerical data Disease prevention DNA sequencing E coli Escherichia coli Escherichia coli O157 - genetics Escherichia coli O157 - isolation & purification Escherichia coli O157 - physiology Female Gene sequencing Genes Genes, Bacterial - genetics Genomes Genomics Geography Hemolytic uremic syndrome Hemolytic-Uremic Syndrome - epidemiology Hemolytic-Uremic Syndrome - microbiology Humans Infectious diseases Interleukin 6 Interleukin 8 Japan - epidemiology Laboratories Leukocytes (mononuclear) Life sciences Male Medicine Middle Aged Mitomycin Mitomycin C Molecular Sequence Data Multilocus sequence typing Nucleotide sequence Outbreaks Peripheral blood mononuclear cells Phylogeny Sequence Analysis, DNA Shiga toxin Shiga toxin 2 Shiga Toxin 2 - genetics Shiga Toxin 2 - metabolism Toxins Virulence Young Adult |
| title | A novel Escherichia coli O157:H7 clone causing a major hemolytic uremic syndrome outbreak in China |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-31T06%3A34%3A18IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-gale_plos_&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=A%20novel%20Escherichia%20coli%20O157:H7%20clone%20causing%20a%20major%20hemolytic%20uremic%20syndrome%20outbreak%20in%20China&rft.jtitle=PloS%20one&rft.au=Xiong,%20Yanwen&rft.date=2012-04-27&rft.volume=7&rft.issue=4&rft.spage=e36144&rft.epage=e36144&rft.pages=e36144-e36144&rft.issn=1932-6203&rft.eissn=1932-6203&rft_id=info:doi/10.1371/journal.pone.0036144&rft_dat=%3Cgale_plos_%3EA477034035%3C/gale_plos_%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=1324600174&rft_id=info:pmid/22558360&rft_galeid=A477034035&rft_doaj_id=oai_doaj_org_article_9363875088b745fea927e4d69f572977&rfr_iscdi=true |