Pseudomonas viridiflava, a multi host plant pathogen with significant genetic variation at the molecular level
The pectinolytic species Pseudomonas viridiflava has a wide host range among plants, causing foliar and stem necrotic lesions and basal stem and root rots. However, little is known about the molecular evolution of this species. In this study we investigated the intraspecies genetic variation of P. v...
Gespeichert in:
| Veröffentlicht in: | PloS one 2012-04, Vol.7 (4), p.e36090-e36090 |
|---|---|
| Hauptverfasser: | , , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | e36090 |
|---|---|
| container_issue | 4 |
| container_start_page | e36090 |
| container_title | PloS one |
| container_volume | 7 |
| creator | Sarris, Panagiotis F Trantas, Emmanouil A Mpalantinaki, Evaggelia Ververidis, Filippos Goumas, Dimitrios E |
| description | The pectinolytic species Pseudomonas viridiflava has a wide host range among plants, causing foliar and stem necrotic lesions and basal stem and root rots. However, little is known about the molecular evolution of this species. In this study we investigated the intraspecies genetic variation of P. viridiflava amongst local (Cretan), as well as international isolates of the pathogen. The genetic and phenotypic variability were investigated by molecular fingerprinting (rep-PCR) and partial sequencing of three housekeeping genes (gyrB, rpoD and rpoB), and by biochemical and pathogenicity profiling. The biochemical tests and pathogenicity profiling did not reveal any variability among the isolates studied. However, the molecular fingerprinting patterns and housekeeping gene sequences clearly differentiated them. In a broader phylogenetic comparison of housekeeping gene sequences deposited in GenBank, significant genetic variability at the molecular level was found between isolates of P. viridiflava originated from different host species as well as among isolates from the same host. Our results provide a basis for more comprehensive understanding of the biology, sources and shifts in genetic diversity and evolution of P. viridiflava populations and should support the development of molecular identification tools and epidemiological studies in diseases caused by this species. |
| doi_str_mv | 10.1371/journal.pone.0036090 |
| format | Article |
| fullrecord | <record><control><sourceid>gale_plos_</sourceid><recordid>TN_cdi_plos_journals_1324600116</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><galeid>A477034100</galeid><doaj_id>oai_doaj_org_article_0d7fef1b9f6e422299ed00bf1a59d6e4</doaj_id><sourcerecordid>A477034100</sourcerecordid><originalsourceid>FETCH-LOGICAL-c692t-6fbf3abe9a28b5c32d5ff9da7e4af2b77579b4a22c3565e4bf3f19f660a39a7a3</originalsourceid><addsrcrecordid>eNqNk22L1DAQx4so3nn6DUQDgii4ax76sHkjHIcPCwcnPr0N03ayzZI2e0266rc3dXvHVu6FBJIw-c1_MpNMkjxldMlEwd5u3dB3YJc71-GSUpFTSe8lp0wKvsg5FfeP9ifJI--3lGZilecPkxPOs2wlUnGadJ89DrVrXQee7E1vaqMt7OENAdIONhjSOB_IzkIXZwiN22BHfprQEG82ndGmGk-iEYOpyB56A8G4jkAgoUHSOovVYKEnFvdoHycPNFiPT6b1LPn-4f23i0-Ly6uP64vzy0WVSx4WuS61gBIl8FWZVYLXmdayhgJT0LwsiqyQZQqcVyLLM0wjrZnUeU5BSChAnCXPD7o767yaSuUVEzzNKWUsj8T6QNQOtmrXmxb638qBUX8Nrt8o6GNKFhWtC42alTEAppxzKbGmtNQMMllHU9R6N0UbyhbrCrvQg52Jzk8606iN2ysh4oOkNAq8mgR6dz2gD6o1vkIbq45uiPeOV2YrmReriL74B707u4naQEzAdNrFuNUoqs7ToqAiZXQMu7yDiqPG1lTxX2kT7TOH1zOHyAT8FTYweK_WX7_8P3v1Y86-PGIbBBsa7-ww_iQ_B9MDWPXO-x71bZEZVWNb3FRDjW2hpraIbs-OH-jW6aYPxB_ungm4</addsrcrecordid><sourcetype>Open Website</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>1324600116</pqid></control><display><type>article</type><title>Pseudomonas viridiflava, a multi host plant pathogen with significant genetic variation at the molecular level</title><source>MEDLINE</source><source>DOAJ Directory of Open Access Journals</source><source>Public Library of Science (PLoS) Journals Open Access</source><source>EZB-FREE-00999 freely available EZB journals</source><source>PubMed Central</source><source>Free Full-Text Journals in Chemistry</source><creator>Sarris, Panagiotis F ; Trantas, Emmanouil A ; Mpalantinaki, Evaggelia ; Ververidis, Filippos ; Goumas, Dimitrios E</creator><contributor>Zhang, Zhengguang</contributor><creatorcontrib>Sarris, Panagiotis F ; Trantas, Emmanouil A ; Mpalantinaki, Evaggelia ; Ververidis, Filippos ; Goumas, Dimitrios E ; Zhang, Zhengguang</creatorcontrib><description>The pectinolytic species Pseudomonas viridiflava has a wide host range among plants, causing foliar and stem necrotic lesions and basal stem and root rots. However, little is known about the molecular evolution of this species. In this study we investigated the intraspecies genetic variation of P. viridiflava amongst local (Cretan), as well as international isolates of the pathogen. The genetic and phenotypic variability were investigated by molecular fingerprinting (rep-PCR) and partial sequencing of three housekeeping genes (gyrB, rpoD and rpoB), and by biochemical and pathogenicity profiling. The biochemical tests and pathogenicity profiling did not reveal any variability among the isolates studied. However, the molecular fingerprinting patterns and housekeeping gene sequences clearly differentiated them. In a broader phylogenetic comparison of housekeeping gene sequences deposited in GenBank, significant genetic variability at the molecular level was found between isolates of P. viridiflava originated from different host species as well as among isolates from the same host. Our results provide a basis for more comprehensive understanding of the biology, sources and shifts in genetic diversity and evolution of P. viridiflava populations and should support the development of molecular identification tools and epidemiological studies in diseases caused by this species.</description><identifier>ISSN: 1932-6203</identifier><identifier>EISSN: 1932-6203</identifier><identifier>DOI: 10.1371/journal.pone.0036090</identifier><identifier>PMID: 22558343</identifier><language>eng</language><publisher>United States: Public Library of Science</publisher><subject>Analysis ; Bacteria ; Base Sequence ; Biochemical tests ; Biodiversity ; Biological effects ; Biological evolution ; Biology ; Carbohydrates ; Chemical fingerprinting ; Deoxyribonucleic acid ; DNA ; DNA Fingerprinting ; DNA topoisomerase ; Electrophoresis, Agar Gel ; Epidemiology ; Evolution ; Evolutionary biology ; Fines & penalties ; Flowers & plants ; Gene sequencing ; Genes ; Genes, Bacterial - genetics ; Genetic aspects ; Genetic diversity ; Genetic variability ; Genetic Variation ; Greenhouses ; Host plants ; Host range ; Host Specificity - genetics ; Host-Pathogen Interactions - genetics ; Identification ; Lesions ; Metabolism ; Molecular evolution ; Pathogenicity ; Pathogens ; Phylogenetics ; Phylogeny ; Plant Diseases - microbiology ; Plant sciences ; Plants (botany) ; Plants - microbiology ; Polymerase Chain Reaction ; Profiling ; Pseudomonas ; Pseudomonas - genetics ; Pseudomonas - isolation & purification ; Pseudomonas syringae ; RpoB protein ; Solanum melongena ; Species ; Studies ; Taxonomy ; Variability</subject><ispartof>PloS one, 2012-04, Vol.7 (4), p.e36090-e36090</ispartof><rights>COPYRIGHT 2012 Public Library of Science</rights><rights>2012 Sarris et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License: https://creativecommons.org/licenses/by/4.0/ (the “License”), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><rights>Sarris et al. 2012</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c692t-6fbf3abe9a28b5c32d5ff9da7e4af2b77579b4a22c3565e4bf3f19f660a39a7a3</citedby><cites>FETCH-LOGICAL-c692t-6fbf3abe9a28b5c32d5ff9da7e4af2b77579b4a22c3565e4bf3f19f660a39a7a3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC3338640/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC3338640/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,727,780,784,864,885,2102,2928,23866,27924,27925,53791,53793</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/22558343$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><contributor>Zhang, Zhengguang</contributor><creatorcontrib>Sarris, Panagiotis F</creatorcontrib><creatorcontrib>Trantas, Emmanouil A</creatorcontrib><creatorcontrib>Mpalantinaki, Evaggelia</creatorcontrib><creatorcontrib>Ververidis, Filippos</creatorcontrib><creatorcontrib>Goumas, Dimitrios E</creatorcontrib><title>Pseudomonas viridiflava, a multi host plant pathogen with significant genetic variation at the molecular level</title><title>PloS one</title><addtitle>PLoS One</addtitle><description>The pectinolytic species Pseudomonas viridiflava has a wide host range among plants, causing foliar and stem necrotic lesions and basal stem and root rots. However, little is known about the molecular evolution of this species. In this study we investigated the intraspecies genetic variation of P. viridiflava amongst local (Cretan), as well as international isolates of the pathogen. The genetic and phenotypic variability were investigated by molecular fingerprinting (rep-PCR) and partial sequencing of three housekeeping genes (gyrB, rpoD and rpoB), and by biochemical and pathogenicity profiling. The biochemical tests and pathogenicity profiling did not reveal any variability among the isolates studied. However, the molecular fingerprinting patterns and housekeeping gene sequences clearly differentiated them. In a broader phylogenetic comparison of housekeeping gene sequences deposited in GenBank, significant genetic variability at the molecular level was found between isolates of P. viridiflava originated from different host species as well as among isolates from the same host. Our results provide a basis for more comprehensive understanding of the biology, sources and shifts in genetic diversity and evolution of P. viridiflava populations and should support the development of molecular identification tools and epidemiological studies in diseases caused by this species.</description><subject>Analysis</subject><subject>Bacteria</subject><subject>Base Sequence</subject><subject>Biochemical tests</subject><subject>Biodiversity</subject><subject>Biological effects</subject><subject>Biological evolution</subject><subject>Biology</subject><subject>Carbohydrates</subject><subject>Chemical fingerprinting</subject><subject>Deoxyribonucleic acid</subject><subject>DNA</subject><subject>DNA Fingerprinting</subject><subject>DNA topoisomerase</subject><subject>Electrophoresis, Agar Gel</subject><subject>Epidemiology</subject><subject>Evolution</subject><subject>Evolutionary biology</subject><subject>Fines & penalties</subject><subject>Flowers & plants</subject><subject>Gene sequencing</subject><subject>Genes</subject><subject>Genes, Bacterial - genetics</subject><subject>Genetic aspects</subject><subject>Genetic diversity</subject><subject>Genetic variability</subject><subject>Genetic Variation</subject><subject>Greenhouses</subject><subject>Host plants</subject><subject>Host range</subject><subject>Host Specificity - genetics</subject><subject>Host-Pathogen Interactions - genetics</subject><subject>Identification</subject><subject>Lesions</subject><subject>Metabolism</subject><subject>Molecular evolution</subject><subject>Pathogenicity</subject><subject>Pathogens</subject><subject>Phylogenetics</subject><subject>Phylogeny</subject><subject>Plant Diseases - microbiology</subject><subject>Plant sciences</subject><subject>Plants (botany)</subject><subject>Plants - microbiology</subject><subject>Polymerase Chain Reaction</subject><subject>Profiling</subject><subject>Pseudomonas</subject><subject>Pseudomonas - genetics</subject><subject>Pseudomonas - isolation & purification</subject><subject>Pseudomonas syringae</subject><subject>RpoB protein</subject><subject>Solanum melongena</subject><subject>Species</subject><subject>Studies</subject><subject>Taxonomy</subject><subject>Variability</subject><issn>1932-6203</issn><issn>1932-6203</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2012</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><sourceid>DOA</sourceid><recordid>eNqNk22L1DAQx4so3nn6DUQDgii4ax76sHkjHIcPCwcnPr0N03ayzZI2e0266rc3dXvHVu6FBJIw-c1_MpNMkjxldMlEwd5u3dB3YJc71-GSUpFTSe8lp0wKvsg5FfeP9ifJI--3lGZilecPkxPOs2wlUnGadJ89DrVrXQee7E1vaqMt7OENAdIONhjSOB_IzkIXZwiN22BHfprQEG82ndGmGk-iEYOpyB56A8G4jkAgoUHSOovVYKEnFvdoHycPNFiPT6b1LPn-4f23i0-Ly6uP64vzy0WVSx4WuS61gBIl8FWZVYLXmdayhgJT0LwsiqyQZQqcVyLLM0wjrZnUeU5BSChAnCXPD7o767yaSuUVEzzNKWUsj8T6QNQOtmrXmxb638qBUX8Nrt8o6GNKFhWtC42alTEAppxzKbGmtNQMMllHU9R6N0UbyhbrCrvQg52Jzk8606iN2ysh4oOkNAq8mgR6dz2gD6o1vkIbq45uiPeOV2YrmReriL74B707u4naQEzAdNrFuNUoqs7ToqAiZXQMu7yDiqPG1lTxX2kT7TOH1zOHyAT8FTYweK_WX7_8P3v1Y86-PGIbBBsa7-ww_iQ_B9MDWPXO-x71bZEZVWNb3FRDjW2hpraIbs-OH-jW6aYPxB_ungm4</recordid><startdate>20120427</startdate><enddate>20120427</enddate><creator>Sarris, Panagiotis F</creator><creator>Trantas, Emmanouil A</creator><creator>Mpalantinaki, Evaggelia</creator><creator>Ververidis, Filippos</creator><creator>Goumas, Dimitrios E</creator><general>Public Library of Science</general><general>Public Library of Science (PLoS)</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>IOV</scope><scope>ISR</scope><scope>3V.</scope><scope>7QG</scope><scope>7QL</scope><scope>7QO</scope><scope>7RV</scope><scope>7SN</scope><scope>7SS</scope><scope>7T5</scope><scope>7TG</scope><scope>7TM</scope><scope>7U9</scope><scope>7X2</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>8AO</scope><scope>8C1</scope><scope>8FD</scope><scope>8FE</scope><scope>8FG</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABJCF</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>ARAPS</scope><scope>ATCPS</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BGLVJ</scope><scope>BHPHI</scope><scope>C1K</scope><scope>CCPQU</scope><scope>D1I</scope><scope>DWQXO</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>H94</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>KB.</scope><scope>KB0</scope><scope>KL.</scope><scope>L6V</scope><scope>LK8</scope><scope>M0K</scope><scope>M0S</scope><scope>M1P</scope><scope>M7N</scope><scope>M7P</scope><scope>M7S</scope><scope>NAPCQ</scope><scope>P5Z</scope><scope>P62</scope><scope>P64</scope><scope>PATMY</scope><scope>PDBOC</scope><scope>PIMPY</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>PTHSS</scope><scope>PYCSY</scope><scope>RC3</scope><scope>7X8</scope><scope>5PM</scope><scope>DOA</scope></search><sort><creationdate>20120427</creationdate><title>Pseudomonas viridiflava, a multi host plant pathogen with significant genetic variation at the molecular level</title><author>Sarris, Panagiotis F ; Trantas, Emmanouil A ; Mpalantinaki, Evaggelia ; Ververidis, Filippos ; Goumas, Dimitrios E</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c692t-6fbf3abe9a28b5c32d5ff9da7e4af2b77579b4a22c3565e4bf3f19f660a39a7a3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2012</creationdate><topic>Analysis</topic><topic>Bacteria</topic><topic>Base Sequence</topic><topic>Biochemical tests</topic><topic>Biodiversity</topic><topic>Biological effects</topic><topic>Biological evolution</topic><topic>Biology</topic><topic>Carbohydrates</topic><topic>Chemical fingerprinting</topic><topic>Deoxyribonucleic acid</topic><topic>DNA</topic><topic>DNA Fingerprinting</topic><topic>DNA topoisomerase</topic><topic>Electrophoresis, Agar Gel</topic><topic>Epidemiology</topic><topic>Evolution</topic><topic>Evolutionary biology</topic><topic>Fines & penalties</topic><topic>Flowers & plants</topic><topic>Gene sequencing</topic><topic>Genes</topic><topic>Genes, Bacterial - genetics</topic><topic>Genetic aspects</topic><topic>Genetic diversity</topic><topic>Genetic variability</topic><topic>Genetic Variation</topic><topic>Greenhouses</topic><topic>Host plants</topic><topic>Host range</topic><topic>Host Specificity - genetics</topic><topic>Host-Pathogen Interactions - genetics</topic><topic>Identification</topic><topic>Lesions</topic><topic>Metabolism</topic><topic>Molecular evolution</topic><topic>Pathogenicity</topic><topic>Pathogens</topic><topic>Phylogenetics</topic><topic>Phylogeny</topic><topic>Plant Diseases - microbiology</topic><topic>Plant sciences</topic><topic>Plants (botany)</topic><topic>Plants - microbiology</topic><topic>Polymerase Chain Reaction</topic><topic>Profiling</topic><topic>Pseudomonas</topic><topic>Pseudomonas - genetics</topic><topic>Pseudomonas - isolation & purification</topic><topic>Pseudomonas syringae</topic><topic>RpoB protein</topic><topic>Solanum melongena</topic><topic>Species</topic><topic>Studies</topic><topic>Taxonomy</topic><topic>Variability</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Sarris, Panagiotis F</creatorcontrib><creatorcontrib>Trantas, Emmanouil A</creatorcontrib><creatorcontrib>Mpalantinaki, Evaggelia</creatorcontrib><creatorcontrib>Ververidis, Filippos</creatorcontrib><creatorcontrib>Goumas, Dimitrios E</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Gale In Context: Opposing Viewpoints</collection><collection>Gale In Context: Science</collection><collection>ProQuest Central (Corporate)</collection><collection>Animal Behavior Abstracts</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Biotechnology Research Abstracts</collection><collection>Nursing & Allied Health Database</collection><collection>Ecology Abstracts</collection><collection>Entomology Abstracts (Full archive)</collection><collection>Immunology Abstracts</collection><collection>Meteorological & Geoastrophysical Abstracts</collection><collection>Nucleic Acids Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>Agricultural Science Collection</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Medical Database (Alumni Edition)</collection><collection>ProQuest Pharma Collection</collection><collection>Public Health Database</collection><collection>Technology Research Database</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Technology Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>Materials Science & Engineering Collection</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest Central UK/Ireland</collection><collection>Advanced Technologies & Aerospace Collection</collection><collection>Agricultural & Environmental Science Collection</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Technology Collection</collection><collection>Natural Science Collection</collection><collection>Environmental Sciences and Pollution Management</collection><collection>ProQuest One Community College</collection><collection>ProQuest Materials Science Collection</collection><collection>ProQuest Central Korea</collection><collection>Engineering Research Database</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Materials Science Database</collection><collection>Nursing & Allied Health Database (Alumni Edition)</collection><collection>Meteorological & Geoastrophysical Abstracts - Academic</collection><collection>ProQuest Engineering Collection</collection><collection>ProQuest Biological Science Collection</collection><collection>Agricultural Science Database</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biological Science Database</collection><collection>Engineering Database</collection><collection>Nursing & Allied Health Premium</collection><collection>Advanced Technologies & Aerospace Database</collection><collection>ProQuest Advanced Technologies & Aerospace Collection</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Environmental Science Database</collection><collection>Materials Science Collection</collection><collection>Access via ProQuest (Open Access)</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><collection>Engineering Collection</collection><collection>Environmental Science Collection</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><collection>DOAJ Directory of Open Access Journals</collection><jtitle>PloS one</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Sarris, Panagiotis F</au><au>Trantas, Emmanouil A</au><au>Mpalantinaki, Evaggelia</au><au>Ververidis, Filippos</au><au>Goumas, Dimitrios E</au><au>Zhang, Zhengguang</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Pseudomonas viridiflava, a multi host plant pathogen with significant genetic variation at the molecular level</atitle><jtitle>PloS one</jtitle><addtitle>PLoS One</addtitle><date>2012-04-27</date><risdate>2012</risdate><volume>7</volume><issue>4</issue><spage>e36090</spage><epage>e36090</epage><pages>e36090-e36090</pages><issn>1932-6203</issn><eissn>1932-6203</eissn><abstract>The pectinolytic species Pseudomonas viridiflava has a wide host range among plants, causing foliar and stem necrotic lesions and basal stem and root rots. However, little is known about the molecular evolution of this species. In this study we investigated the intraspecies genetic variation of P. viridiflava amongst local (Cretan), as well as international isolates of the pathogen. The genetic and phenotypic variability were investigated by molecular fingerprinting (rep-PCR) and partial sequencing of three housekeeping genes (gyrB, rpoD and rpoB), and by biochemical and pathogenicity profiling. The biochemical tests and pathogenicity profiling did not reveal any variability among the isolates studied. However, the molecular fingerprinting patterns and housekeeping gene sequences clearly differentiated them. In a broader phylogenetic comparison of housekeeping gene sequences deposited in GenBank, significant genetic variability at the molecular level was found between isolates of P. viridiflava originated from different host species as well as among isolates from the same host. Our results provide a basis for more comprehensive understanding of the biology, sources and shifts in genetic diversity and evolution of P. viridiflava populations and should support the development of molecular identification tools and epidemiological studies in diseases caused by this species.</abstract><cop>United States</cop><pub>Public Library of Science</pub><pmid>22558343</pmid><doi>10.1371/journal.pone.0036090</doi><tpages>e36090</tpages><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 1932-6203 |
| ispartof | PloS one, 2012-04, Vol.7 (4), p.e36090-e36090 |
| issn | 1932-6203 1932-6203 |
| language | eng |
| recordid | cdi_plos_journals_1324600116 |
| source | MEDLINE; DOAJ Directory of Open Access Journals; Public Library of Science (PLoS) Journals Open Access; EZB-FREE-00999 freely available EZB journals; PubMed Central; Free Full-Text Journals in Chemistry |
| subjects | Analysis Bacteria Base Sequence Biochemical tests Biodiversity Biological effects Biological evolution Biology Carbohydrates Chemical fingerprinting Deoxyribonucleic acid DNA DNA Fingerprinting DNA topoisomerase Electrophoresis, Agar Gel Epidemiology Evolution Evolutionary biology Fines & penalties Flowers & plants Gene sequencing Genes Genes, Bacterial - genetics Genetic aspects Genetic diversity Genetic variability Genetic Variation Greenhouses Host plants Host range Host Specificity - genetics Host-Pathogen Interactions - genetics Identification Lesions Metabolism Molecular evolution Pathogenicity Pathogens Phylogenetics Phylogeny Plant Diseases - microbiology Plant sciences Plants (botany) Plants - microbiology Polymerase Chain Reaction Profiling Pseudomonas Pseudomonas - genetics Pseudomonas - isolation & purification Pseudomonas syringae RpoB protein Solanum melongena Species Studies Taxonomy Variability |
| title | Pseudomonas viridiflava, a multi host plant pathogen with significant genetic variation at the molecular level |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2024-12-23T18%3A49%3A32IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-gale_plos_&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Pseudomonas%20viridiflava,%20a%20multi%20host%20plant%20pathogen%20with%20significant%20genetic%20variation%20at%20the%20molecular%20level&rft.jtitle=PloS%20one&rft.au=Sarris,%20Panagiotis%20F&rft.date=2012-04-27&rft.volume=7&rft.issue=4&rft.spage=e36090&rft.epage=e36090&rft.pages=e36090-e36090&rft.issn=1932-6203&rft.eissn=1932-6203&rft_id=info:doi/10.1371/journal.pone.0036090&rft_dat=%3Cgale_plos_%3EA477034100%3C/gale_plos_%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=1324600116&rft_id=info:pmid/22558343&rft_galeid=A477034100&rft_doaj_id=oai_doaj_org_article_0d7fef1b9f6e422299ed00bf1a59d6e4&rfr_iscdi=true |